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    KROMATOGRAFI

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    Ana Yustica
    Chromatography separates the components of a mixture based on their interaction with a mobile and stationary phase. There are several types of chromatography including adsorption, partition, ion exchange, molecular exclusion, and affinity. Chromatography techniques are classified based on the mobile phase used, such as liquid chromatography which uses a liquid mobile phase, gas chromatography which uses a gas mobile phase, and supercritical fluid chromatography which uses a supercritical fluid mobile phase. Key terms in chromatography include retention time, retention volume, distribution constant, retention factor, selectivity factor, column efficiency, and resolution.

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    KROMATOGRAFI

    Uploaded by

    Ana Yustica
    170 views18 pages
    Chromatography separates the components of a mixture based on their interaction with a mobile and stationary phase. There are several types of chromatography including adsorption, partition, ion exchange, molecular exclusion, and affinity. Chromatography techniques are classified based on the mobile phase used, such as liquid chromatography which uses a liquid mobile phase, gas chromatography which uses a gas mobile phase, and supercritical fluid chromatography which uses a supercritical fluid mobile phase. Key terms in chromatography include retention time, retention volume, distribution constant, retention factor, selectivity factor, column efficiency, and resolution.

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    Chromatography separates the components of a mixture based on their interaction with a mobile and stationary phase. There are several types of chromatography including adsorption, partition, ion exchange, molecular exclusion, and affinity. Chromatography techniques are classified based on the mobile phase used, such as liquid chromatography which uses a liquid mobile phase, gas chromatography which uses a gas mobile phase, and supercritical fluid chromatography which uses a supercritical fluid mobile phase. Key terms in chromatography include retention time, retention volume, distribution constant, retention factor, selectivity factor, column efficiency, and resolution.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    170 views18 pages

    KROMATOGRAFI

    Uploaded by

    Ana Yustica
    Chromatography separates the components of a mixture based on their interaction with a mobile and stationary phase. There are several types of chromatography including adsorption, partition, ion exchange, molecular exclusion, and affinity. Chromatography techniques are classified based on the mobile phase used, such as liquid chromatography which uses a liquid mobile phase, gas chromatography which uses a gas mobile phase, and supercritical fluid chromatography which uses a supercritical fluid mobile phase. Key terms in chromatography include retention time, retention volume, distribution constant, retention factor, selectivity factor, column efficiency, and resolution.

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    Download as pptx, pdf, or txt
    of 18

    KROMATOGRAFI

    Definition of Chromatography
    Simplified Definition:

    Chromatography separates the components of a mixture by their distinctive attraction to the mobile phase and the stationary phase.
    Explanation:

    Mobile phase passes through the stationary phase Mobile phase solubilizes the components Mobile phase carries the individual components a certain distance through the stationary phase, depending on their attraction to both of the phases

    Separation mechanism (interaction between solute and stationary phase) in chromatography:

    1. Adsorption
    Using solid stationary phase and liquid or gas mobile phase. Solute was adsorbed in solid particle surface.

    the relative polarities of solute and solid stationary phase determine the rate of movement of that solute through a column or across a surface

    2. Partition
    Stationary phase : liquid (sorbed solvent held on the surface, or within the grains or fibres of an inert solid supporting matrix)

    3. Ion exchange
    The stationary phase is a permeable polymeric solid containing fixed charged groups and mobile counter-ions which can exchange with the ions of a solute as the mobile phase carries them through the structure. Anion like SO3- (as cation exchange), or cation like N(CH3)3+ (as anion exchange) hold covalently in solid stationary phase , commonly: resin (polymer). Solute ion that have opposite charge will bonded in stationary phase by electrostatic force.

    4. molecular exclusion
    called gel filtration atau gel permeation chrom. This technique separate solute based on the size, the smaller solute will more retain.

    5. Affinity
    Involve specific interaction between one kind of solute molecule and the second molecule that bonded covalently (immobilized) by stationary phase. For example, the immobilized molecule may be an antibody to some specific protein. When solute containing a mixture of proteins are passed by this molecule, only the specific protein is reacted to this antibody, binding it to the stationary phase. This protein is later extracted by changing the ionic strength or pH.

    Klasifikasi metode kromatografi kolom


    Klasifikasi Metode/ mekanisme Liquid Chromatography Liquid-liquid or (LC) partition mobile phase: liquid Liquid-bonded phase Liquid-solid or adsorption Ion exchange Size exclusion Gas chromatography (GC) Mobile phase: gas Gas-liquid Gas-bonded phase Gas-solid Supercritical fluid chromatography (SFC) Mobile phase: supercritical fluid Fs diam Liquid adsorbed on a solid Organic species bonded to a solid surface Solid Ion-exchange solid Liquid in intersticies of a polymeric solid Liquid adsorbed on a solid Organic species bonded to a solid surface Solid Organic species bonded to a solid surface Jenis kesetimbangan Partition between immiscible liquids Partition between liquid & bonded surface Adsorption Ion exchange Partition/ sieving Partition between gas & liquid Partition between gas & bonded surface Adsorption Partition between supercritical fluid & bonded surface

    kromatogram

    Konstanta distribusi
    waktu retensi, tR tM ; tR Volum retensi Laju rata-rata solut dlm kolom Laju rata-rata fase gerak dalam kolom

    Hubungan waktu retensi & konstanta distribusi

    dengan kA: faktor retensi atau kapasitas faktor

    Faktor retensi

    Faktor selektivitas

    efisiensi kolom
    Teori lempeng (plate theory) N=L/H N: jumlah lempeng teoritik L: panjang kolom H: height equivalent of theoretical plates (HETP), tinggi ekivalen lempeng teoritik, tebal lempeng teoritik

    Teori kinetik (kinetics theory)


    N = L/H Tinggi plat dengan Banyaknya plat

    Resolusi kolom (Rs) menunjukkan perhitungan secara kuantitatif kemampuan suatu kolom untuk memisahkan 2 analit

    Pengaruh retensi & factor selektivitas terhadap resolusi Dengan asumsi


    maka

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