Third party distribution of this cell line is discouraged. Storage at -70degC will result in loss of viability. ATCC highly recommends that protective gloves and clothing always be used.
Third party distribution of this cell line is discouraged. Storage at -70degC will result in loss of viability. ATCC highly recommends that protective gloves and clothing always be used.
Third party distribution of this cell line is discouraged. Storage at -70degC will result in loss of viability. ATCC highly recommends that protective gloves and clothing always be used.
Third party distribution of this cell line is discouraged. Storage at -70degC will result in loss of viability. ATCC highly recommends that protective gloves and clothing always be used.
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Product Information Sheet for CRL-1682
Cell Line Designation: AsPC-1 ATCC Catalog No. CRL-1682
Table of Contents: Cell Line Description Biosafety Level Use Restrictions Handling Procedure for Frozen Cells Handling Procedure for Flask Cultures Subculturing Procedure Medium Renewal Complete Growth Medium Cryoprotectant Medium References Replacement Policy Specific Batch Information an agreement. Third party distribution of this cell line is discouraged, since this practice has resulted in the unintentional spreading of cell lines contaminated with inappropriate animal cells or microbes.
Handling Procedure for Frozen Cells
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at 70C. Storage at 70C will result in loss of viability. SAFETY PRECAUTION: ATCC highly recommends that protective gloves and clothing always be used and a full face mask always be worn when handling frozen vials. It is important to note that some vials leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris. 1. Thaw the vial by gentle agitation in a 37C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9.0 ml complete growth medium and spin at approximately 125 xg for 5 to 7 minutes. Resuspend cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). Incubate the culture at 37C in a suitable incubator. A 5% CO 2 in air atmosphere is recommended if using the medium described on this product sheet.
Cell Line Description
Organism: Homo sapiens (human) Tissue: adenocarcinoma; pancreas; derived from metastatic site: ascites Age: 62 years Gender: female Ethnicity: Caucasian Morphology: epithelial Products: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin Growth Properties: adherent DNA Profile (STR analysis): Amelogenin: X CSF1PO: 10,13 D13S317: 9,12 D16S539: 11 D5S818: 12 D7S820: 12,13 TH01: 7,9.3 TPOX: 8,10 vWA: 17 Depositors: M.H. Tan Comments: The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas. Biosafety Level: 1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 4th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 1999. The entire text is available online at www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm
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Handling Procedure For Flask Cultures
The flask was seeded with cells (see specific batch information) grown and completely filled with medium at ATCC to prevent loss of cells during shipping.
Use Restrictions These cells are distributed for research purposes only. ATCC recommends that individuals contemplating commercial use of any cell line first contact the originating investigator to negotiate American Type Culture Collection P.O. Box 1549 Manassas, VA 20108 USA www.atcc.org
800-638-6597 (U.S., Canada, and Puerto Rico) 703-365-2700 Fax: 703-365-2750 E-mail: [email protected]
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Product Information Sheet for CRL-1682
1. Upon receipt visually examine the culture for macroscopic evidence of any microbial contamination. Using an inverted microscope (preferably equipped with phase-contrast optics), carefully check for any evidence of microbial contamination. Also check to determine if the majority of cells are still attached to the bottom of the flask; during shipping the cultures are sometimes handled roughly and many of the cells often detach and become suspended in the culture medium (but are still viable). If the cells are still attached, aseptically remove all but 5 to 10 ml of the shipping medium. The shipping medium can be saved for reuse. Incubate the cells at 37C in a 5% CO 2 in air atmosphere until they are ready to be subcultured. If the cells are not attached, aseptically remove the entire contents of the flask and centrifuge at 125 xg for 5 to 10 minutes. Remove shipping medium and save. Resuspend the pelleted cells in 10 ml of this medium and add to 25 cm2 flask. Incubate at 37C in a 5% CO 2 in air atmosphere until cells are ready to be subcultured. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO 2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
Cryoprotectant Medium Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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Additional Information Additional product and technical information can be obtained from the catalog references and the ATCC Web site at www.atcc.org, or by e-mail at [email protected].
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References (additional references are available in the catalog at www.atcc.org) Chen WH et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982 PubMed: 83211801 Tan MH et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981 PubMed: 82011428 Loor R et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567578, 1982 PubMed: 83051943 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Subculturing Procedure Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. Subcultivation Ratio: 1:3 to 1:6. Incubate cultures at 37C.
ATCC Warranty The viability of ATCC products is warranted for 30 days from the date of shipment. If you feel there is a problem with this product, contact Technical Services by phone at 800-638-6597 (U.S., Canada, and Puerto Rico) or 703-365-2700 (elsewhere) or by email at [email protected].
Disclaimers This product is intended for laboratory research purposes only. It is not intended for use in humans. While ATCC uses reasonable efforts to include accurate and up-todate information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, and use. ATCC is not liable for any damages or injuries arising from receipt and/or use of this product.
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Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Medium Renewal 2 to 3 times weekly.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI1640 Medium, Catalog No. 30-2001. American Type Culture Collection P.O. Box 1549 Manassas, VA 20108 USA www.atcc.org
800-638-6597 (U.S., Canada, and Puerto Rico) 703-365-2700 Fax: 703-365-2750 E-mail: [email protected]
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Product Information Sheet for CRL-1682
While reasonable effort is made to insure authenticity and reliability of strains on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this product. The MTA is also available on our Web site at www.atcc.org. ATCC 2007. All rights reserved. ATCC is a registered trademark of the American Type Culture Collection. 07/07
American Type Culture Collection P.O. Box 1549 Manassas, VA 20108 USA www.atcc.org
800-638-6597 (U.S., Canada, and Puerto Rico) 703-365-2700 Fax: 703-365-2750 E-mail: [email protected]
