ENVSC Lab Part 1
ENVSC Lab Part 1
6. How to sampling?
Wear gloves and eye protection when collecting samples. Rinse the bottle and cap three times with sample
water and fill the bottle to within one to two inches from the top. Place the sample into a cooler with ice for
immediate delivery or shipment to the laboratory. Sterile 125 or 150 mL plastic bottles must be used
7. How to make a solution at lab?
8. How to measure dose amount in labs?
9. Basic apparatus of lab?
10.How to measure noise ?
11.How to prepare solution
12.What is pH
• Solutions with a pH value varying from 0 to <7 on the pH scale are called acidic solutions.
• Solution with a pH value ranging from >7 to 14 is known as basic solutions.
• On a pH scale, solutions with a potential of hydrogen or a pH value equal to 7 are known
as neutral solutions.
Solutions with a pH value of 0 are considered to be extremely acidic. Additionally, the acidity
decreases as the pH value increase from 0 to 7, while solutions with a pH value equal to 14 are
known to be strongly basic solutions. The acid and base intensity depends on the number of H+ and
OH– ions produced. Acids furnishing more H+ ions are known to be strong acids, and vice versa.
Importance of pH
• A living organism can withstand only a limited range of pH changes, and any more pH
adjustments will make life difficult. For example: in the case of acid rain, the pH of the
water is less than 7. It decreases the pH of river water as it flows into a river which hinders
the survival of marine life.
• The human stomach lining secretes hydrochloric acid, which helps digest food by activating
certain enzymes. Antacids are used to neutralise the acid produced in the stomach in case
of acid reflux.
• Often the bacteria present in our mouth lower the pH of our mouth by generating acids
through food particle degradation. This results in the demineralisation of teeth and tooth
decay. Therefore, we are told to clean our teeth and mouths since mouthwash neutralises
the acid and helps in remineralisation. Fluoride toothpaste aids in remineralisation and
prevents decay.
• In the case of a bee sting, we feel a lot of pain when the bee injects formic acid (methanoic
acid) through its sting. Therefore, we are usually recommended to apply baking soda or
other mild bases to the surface because it helps to neutralise the acid and maintain the pH.
pH, quantitative measure of the acidity or basicity of aqueous or other liquid solutions. The term,
widely used in chemistry, biology, and agronomy, translates the values of the concentration of
the hydrogen ion—which ordinarily ranges between about 1 and 10−14 gram-equivalents per
litre—into numbers between 0 and 14.
In pure water, which is neutral (neither acidic nor alkaline), the concentration of the hydrogen ion
is 10−7 gram-equivalents per litre, which corresponds to a pH of 7. A solution with a pH less than
7 is considered acidic; a solution with a pH greater than 7 is considered basic, or alkaline.
The measurement was originally used by the Danish biochemist S.P.L. Sørensen to represent the
hydrogen ion concentration, expressed in equivalents per litre, of an aqueous solution: pH =
−log[H+] (in expressions of this kind, enclosure of a chemical symbol within
square brackets denotes that the concentration of the symbolized species is the quantity being
considered).