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01 - DNA Extraction

The document provides a step-by-step guide on how to extract DNA from strawberries using common household materials. It explains the importance of DNA as the molecule that controls cellular activities and outlines the necessary ingredients and procedures for the extraction process. The final step involves observing the formation of DNA as a white cloudy substance after adding cold rubbing alcohol to the strawberry extract.

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Md. Abdul Mukit
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6 views10 pages

01 - DNA Extraction

The document provides a step-by-step guide on how to extract DNA from strawberries using common household materials. It explains the importance of DNA as the molecule that controls cellular activities and outlines the necessary ingredients and procedures for the extraction process. The final step involves observing the formation of DNA as a white cloudy substance after adding cold rubbing alcohol to the strawberry extract.

Uploaded by

Md. Abdul Mukit
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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• Cells are the basic unit of

life and make up all plants,


animals and bacteria.
Deoxyribonucleic acid, or
How to Extract DNA from a DNA, is the molecule that
controls everything that
Strawberry happens in the cell. DNA
contains instructions that
direct the activities of cells
and, ultimately, the body.
This activity will
demonstrate how DNA can
be isolated from a
strawberry using common
household materials.
What you will need:

1 re-sealable plastic bag , 2 Strawberries(fresh or frozen), 2tsp Dish detergent , 1/2cup water, 2 Plastic cups
1 Coffee filter, ½ cup COLD Rubbing Alcohol, 1 coffee Stirrer
1 pull off any green leaves on the strawberry that
have not been removed yet.
2 put the strawberries into the plastic bag, seal it
and gently smash it for about two minutes.
Completely crush the strawberries. This starts to
break open the cells and release the DNA
3 In a plastic cup, make your DNA extraction
liquid: mix together 2 teaspoons of detergent, 1
teaspoon of salt and ½ cup of water
4 Add 2 teaspoons of the DNA extraction liquid
into the bag with the strawberries. This will
further break open the cells.
5 Reseal the bag and gently smash for another
minute(avoid making too many soap bubbles).
6 and 7
place the coffee filter inside the other plastic cup.
Open the bag and pour the strawberry liquid into filter. You can
twist the filter just above the liquid and gently squeeze the
remaining liquid into the cup.
8 Next, pour down the side of the an equal amount of
cold rubbing alcohol as there is strawberry liquid. Do
not mix or stir. You have just isolated the DNA from the
rest of the material contained in the cells of the
strawberry.
9 within a few seconds, watch for the development of a
white cloudy substance(DNA) in the top layer above the
strawberry extract layer.
10 Tilt the cup and pick up the DNA using a plastic
coffee stirrer or wooden stick.

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