Bioinformatics: Lab Exercise#6

BLAST EXERCISE: Identification of unknown protein and nucleotide

1. Given a protein sequence, find out which protein it is and from which organism it
belongs using BLAST Program.
MERGVRRGAALVAAWRSLWERGGLALFRPQCRTGCGACRVQGTRPFSLSAAASAVLG
LGSWGGDSGKQKLTLQDVAELIRKKECRRVVVMAGAGISTPSGIPDFRSPGSGLYSNLE
QYNIPYPEAIFELAYFFINPKPFFTLAKELYPGNYRPNYAHYFLRLLHDKGLLLRLYTQNI
DGLERVAGIPPDRLVEAHGTFATATCTVCRRKFPGEDFRGDVMADKVPHCRVCTGIVK
PDIVFFGEELPQRFFLHMTDFPMADLLFVIGTSLEVEPFASLAGAVRNSVPRVLINR
DLVGPFAWQQRYNDIAQLGDVVTGVEKMVELLDWNEEMQTLIQKEKEKLDAKDK
From the given details, find
 Name the protein of that sequence.

 What is the length of the query sequence ?

 What is the number of sequences in the database searched ?

The E-value (Expect Value) is a statistical measure used in sequence alignment (such as
BLAST) to indicate the number of matches (or alignments) you might expect to see by chance
when searching a database.
 A low E-value (close to 0) indicates a highly significant match, meaning the alignment
is unlikely to have occurred by chance.
 A high E-value suggests the match could be due to random chance and is less significant.
In summary, the E-value helps assess the quality and reliability of the alignment result:
 E-value ≈ 0: Strong, significant match.
 E-value > 0: Weaker, less significant match.
The lower the E-value, the more reliable the match between the sequences.

2. Given a nucleotide sequence, find the details regarding the sequence like which
organism, name, accession id etc. using BLAST

Nucleotide Sequence

AGTGCCGCGCGTCGAGCGGAGCAGAGGAGGCGAGGGCGGAGGGCCAGAGAGGCAGTTGGAAGATGGCGGAC
GAGGTGGCGCTCGCCCTTCAGGCCGCCGGCTCCCCTTCCGCGGCGGCCGCCATGGAGGCCGCGTCGCAGCCGGC
GGACGAGCCGCTCCGCAAGAGGCCCCGCCGAGACGGGCCTGGCCTCGGGCGCAGCCCGGGCGAGCCGAGCGCA
GCAGTGGCGCCGGCGGCCGCGGGGTGTGAGGCGGCGAGCGCCGCGGCCCCGGCGGCGCTGTGGCGGGAGGCG
GCAGGGGCGGCGGCGAGCGCGGAGCGGGAGGCCCCGGCGACGGCCGTGGCCGGGGACGGAGACAATGGGTCC
GGCCTGCGGCGGGAGCCGAGGGCGGCTGACGACTTCGACGACGACGAGGGCGAGGAGGAGGACGAGGCGGCG
GCGGCAGCGGCGGCGGCAGCGATCGGCTACCGAGACAACCTCCTGTTGACCGATGGACTCCTCACTAATGGCTTT
CATTCCTGTGAAAGTGATGACGATGACAGAACGTCACACGCCAGCTCTAGTGACTGGACTCCGCGGCCGCGGATA
GGTCCATATACTTTTGTTCAGCAACATCTCATGATTGGCACCGATCCTCGAACAATTCTTAAAGATTTATTACCAGA
AACAATTCCTCCACCTGAGCTGGATGATATGACGCTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAGC
GGAAAAAAAGAAAAGATATCAATACAATTGAAGATGCTGTGAAGTTACTGCAGGAGTGTAAAAAGATAATAGTTC
TGACTGGAGCTGGGGTTTCTGTCTCCTGTGGGATTCCTGACTTCAGATCAAGAGACGGTATCTATGCTCGCCTTGC
GGTGGACTTCCCAGACCTCCCAGACCCTCAAGCCATGTTTGATATTGAGTATTTTAGAAAAGACCCAAGACCATTCT
TCAAGTTTGCAAAGGAAATATATCCCGGACAGTTCCAGCCGTCTCTGTGTCACAAATTCATAGCTTTGTCAGATAA
GGAAGGAAAACTACTTCGAAATTATACTCAAAATATAGATACCTTGGAGCAGGTTGCAGGAATCCAAAGGATCCT
TCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTTGTAAATACAAAGTTGATTGTGAAGCTGTTCGTGGAG
ACATTTTTAATCAGGTAGTTCCTCGGTGCCCTAGGTGCCCAGCTGATGAGCCACTTGCCATCATGAAGCCAGAGAT
TGTCTTCTTTGGTGAAAACTTACCAGAACAGTTTCATAGAGCCATGAAGTATGACAAAGATGAAGTTGACCTCCTC
ATTGTTATTGGATCTTCTCTGAAAGTGAGACCAGTAGCACTAATTCCAAGTTCTATACCCCATGAAGTGCCTCAAAT
ATTAATAAATAGGGAACCTTTGCCTCATCTACATTTTGATGTAGAGCTCCTTGGAGACTGCGATGTTATAATTAATG
AGTTGTGTCATAGGCTAGGTGGTGAATATGCCAAACTTTGTTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAA
ACCTCCACGCCCACAAAAGGAATTGGTTCATTTATCAGAGTTGCCACCAACACCTCTTCATATTTCGGAAGACTCAA
GTTCACCTGAAAGAACTGTACCACAAGACTCTTCTGTGATTGCTACACTTGTAGACCAAGCAACAAACAACAATGT
TAATGATTTAGAAGTATCTGAATCAAGTTGTGTGGAAGAAAAACCACAAGAAGTACAGACTAGTAGGAATGTTGA
GAACATTAATGTGGAAAATCCAGATTTTAAGGCTGTTGGTTCCAGTACTGCAGACAAAAATGAAAGAACTTCAGTT
GCAGAAACAGTGAGAAAATGCTGGCCTAATAGACTTGCAAAGGAGCAGATTAGTAAGCGGCTTGAGGGTAATCA
ATACCTGTTTGTACCACCAAATCGTTACATATTCCACGGTGCTGAGGTATACTCAGACTCTGAAGATGACGTCTTGT
CCTCTAGTTCCTGTGGCAGTAACAGTGACAGTGGCACATGCCAGAGTCCAAGTTTAGAAGAACCCTTGGAAGATG
AAAGTGAAATTGAAGAATTCTACAATGGCTTGGAAGATGATACGGAGAGGCCCGAATGTGCTGGAGGATCTGGA
TTTGGAGCTGATGGAGGGGATCAAGAGGTTGTTAATGAAGCTATAGCTACAAGACAGGAATTGACAGATGTAAA
CTATCCATCAGACAAATCATAACACTATTGAAGCTGTCCGGATTCAGGAATTGCTCCACCAGCATTGGGAACTTTA
GCATGTCAAAAAATGAATGTTTACTTGTGAACTTGAACAAGGAAATCTGAAAGATGTATTATTTATAGACTGGAAA
ATAGATTGTCTTCTTGGATAATTTCTAAAGTTCCATCATTTCTGTTTGTACTTGTACATTCAACACTGTTGGTTGACTT
CATCTTCCTTTCAAGGTTCATTTGTATGATACATTCGTATGTATGTATAATTTTGTTTTTTGCCTAATGAGTTTCAACC
TTTTAAAGTTTTCAAAAGCCATTGGAATGTTAATGTAAAGGGAACAGCTTATCTAGACCAAAGAATGGTATTTCAC
ACTTTTTTGTTTGTAACATTGAATAGTTTAAAGCCCTCAATTTCTGTTCTGCTGAACTTTTATTTTTAGGACAGTTAAC
TTTTTAAACACTGGCATTTTCCAAAACTTGTGGCAGCTAACTTTTTAAAATCACAGATGACTTGTAATGTGAGGAGT
CAGCACCGTGTCTGGAGCACTCAAAACTTGGTGCTCAGTGTGTGAAGCGTACTTACTGCATCGTTTTTGTACTTGCT
GCAGACGTGGTAATGTCCAAACAGGCCCCTGAGACTAATCTGATAAATGATTTGGAAATGTGTTTCAGTTGTTCTA
GAAACAATAGTGCCTGTCTATATAGGTCCCCTTAGTTTGAATATTTGCCATTGTTTAATTAAATACCTATCACTGTGG
TAGAGCCTGCATAGATCTTCACCACAAATACTGCCAAGATGTGAATATGCAAAGCCTTTCTGAATCTAATAATGGT
ACTTCTACTGGGGAGAGTGTAATATTTTGGACTGCTGTTTTTCCATTAATGAGGAAAGCAATAGGCCTCTTAATTAA
AGTCCCAAAGTCATAAGATAAATTGTAGCTCAACCAGAAAGTACACTGTTGCCTGTTGAGGATTTGGTGTAATGTA
TCCCAAGGTGTTAGCCTTGTATTATGGAGATGAATACAGATCCAATAGTCAAATGAAACTAGTTCTTAGTTATTTAA
AAGCTTAGCTTGCCTTAAAACTAGGGATCAATTTTCTCAACTGCAGAAACTTTTAGCCTTTCAAACAGTTCACACCT
CAGAAAGTCAGTATTTATTTTACAGACTTCTTTGGAACATTGCCCCCAAATTTAAATATTCATGTGGGTTTAGTATTT
ATTACAAAAAAATGATTTGAAATATAGCTGTTCTTTATGCATAAAATACCCAGTTAGGACCATTACTGCCAGAGGA
GAAAAGTATTAAGTAGCTCATTTCCCTACCTAAAAGATAACTGAATTTATTTGGCTACACTAAAGAATGCAGTATAT
TTAGTTTTCCATTTGCATGATGTGTTTGTGCTATAGACAATATTTTAAATTGAAAAATTTGTTTTAAATTATTTTTACA
GTGAAGACTGTTTTCAGCTCTTTTTATATTGTACATAGACTTTTATGTAATCTGGCATATGTTTTGTAGACCGTTTAA
TGACTGGATTATCTTCCTCCAACTTTTGAAATACAAAAACAGTGTTTTATACTTGTATCTTGTTTTAAAGTCTTATATT
AAAATTGTCATTTGACTTTTTTCCCGTTAAAAAAAAAAAAAAA

http://vlab.amrita.edu/?sub=3&brch=274&sim=1434&cnt=5

3. find the details regarding the sequence like which