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BIOLOGY
PAPER 3
(PRACTICAL)

1.(a)You are provided with the following:

 Specimen Y
Hydrogen peroxide
 2 test tubes in a test tube rack.
 2 labels
 10ml measuring cylinder.
 A scalpel.
 2 wooden splints.
 100ml beaker.

Procedure

(i) Label two test tubes A and B.

(ii) Measure 2cm3 of hydrogen peroxide and put in test tube A. Repeat the same procedure for
test tube B.

(iii) Cut a small piece of specimen Y to two smaller pieces using a scalpel. Place one of the
pieces in test tube A and retain the other piece for the subsequent procedure for test tube B.

(iv) Immediately, introduce a glowing splint into the mouth of the test tube. Record your
observations in the table below.

(v) Put the other piece of specimen Y in an empty 100ml beaker then add 50ml boiling water
from a hot water bath maintained at 800C. Leave the set up for 5 minutes

(vi) Remove specimen Y from the boiling water using a pair of forceps and place in test tube B.
Immediately, introduce a glowing splint at the mouth of the test tube. Record your observations
in the table below.

(a) Record your observations in this table

Test Observations
tube
On placing specimen Y On introducing a glowing
splint
A More
effervescence/frothing/bubbles Rekindles/relights√
produced√
(1mark)
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B No/less/littleeffervescence/
frothing/bubble produced√ Does not rekindles√

(1mark)

(b) Explain your observations :.

(i) On placing specimen Y on test tube A (2marks)

 Enzyme (catalase) present in the specimen Y breaksdown

hydrogen peroxide into water and oxygen gas;√ hence bubbles
produced;√

(ii) On introducing the glowing splint on test B (2marks)

 Boiling specimen Y denatured the enzyme (catalase);√ hence

fail to break down hydrogen peroxide into water and oxygen
gas√

(c) State the role of experimental set up in test tube B. (1mark)

 Control experiment;√

(d) Specimen Y is an organ in animals. State its one other function apart from the one being

investigated above. (1mark)

 Blood sugar regulation;√

Mark first one
 Osmoregulation

 Regulation of metabolism

 Deamination

(e) You are provided specimen X. Make a longitudinal section through one of the specimen X

using the scalpel to obtain two halves.

(i) Carefully observe one of the halves and make a drawing. on the diagram label the position of

the plumule and radicle. (3marks)

(ii) State one internal factor necessary for the germination of specimen X. (1 mark)

 Hormones;√
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 Enzymes

 viability

(iii) Using a mortar and pestle provided, crush the remaining pieces of X while adding water
to form a solution. Transfer the solution into a 50ml beaker provided and label as solution X.
Using the reagents provided, test for the food substance present in solution X. (3marks)

FOOD PROCEDURE OBSERVATION CONCLUTION

SUBSTANCE

PROTEIN Put 2cm3 of solution Puple/violet Protein

X in a clean test tube observed;√ present;√
.add copper(II)
sulphate drop by
drop by shaking after
each drop ;√

(iv) Under what circumstance is the food present in solution X oxidized in the human body
(1mark)
 Extreme starvation;√
2.Below is a plant organ used in the study of biology. Study it and answer the questions that

follow.

(a) Identify the organ above (1mark)

 Flower;√

(b)State the function of the part labeled H (1mark)

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 Protects the flower during bud stage;√

(c) State the term used to describe the petals. (1mark)

 Polypetalous;√

(d) State with a reason the class into which the organ belongs.

Class (1mark)

Dichotyledonae;√

Reason (1mark)

(e)(i) Using observable feature only, name the argent of pollination (1mark)

 Insects;√

(ii) Give a reason for your answer in (e)(i) above. (1mark)

 Brightly coloured petal to attract the insect;√

(f) State the importance of the organ to a plant (1mark)

Pollination;√

(g) Measure the length of the petal from point A to B. (1mark)

 3.5 cm;√

(f) If the actual length between A and B is 5cm, calculate the magnification of the photograph
above. (2marks)

 Magnification=length of image
Object length

=3.5;√
5 =X 0.7;√
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3.(a) Below are photographs of Venus flytrap (an insectivorous plant). Study them and answer

the questions that follow.

(i)

Name one major nutrient that is deficient in the soil where the above plant grows.(1mark)

 Nitrogen ;√

(ii) Name the type of response shown by plate C (1mark)

 Haptonasty;√

(iii) Describe how the above plant traps the insect (3marks)

 When the sensitive hairs on the leaves are touched by the

landing insect;√the mid rib lose water rapidly by

osmosis;√causing the trap to spring hence closing the leaves

with the spines interlocking;√

(b) Below is a mammalian skeleton. Study it carefully an answer the questions that follow.

(i) Name bone W (1mark)

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 Femur;√

(ii) Name the type joint formed by bone W at the distal end. (1mark)

 Hinge;√

(c) The part labeled V has one major adaptation:

(i) Identify the adaptation (1mark)

 Elastic cartilage;√

(ii) Explain the importance of the adaptation in (c)(i) above to females (1mark)

 Expand during birth in females to allow for the foetus;√

(d) State the function of the part labeled U (1mark)

 Allow for the passage of nerves,blood vessels and muscles;√

(e) Distinguish between pitching and rolling as used in bony fish (1mark)

 Rolling-rocking from side to side

 Pitching-upword and downward movement of the fish;√