Nucleic Acid Extraction System

BNP48
User Manual

BIOBASE GROUP

Version 2020.09
 Preface

Welcome to buy BIOBASE products, thank you!

I sincerely hope that our products can bring the greatest help to your work.
● Please read this manual carefully when using this machine for the first time!
● This product can only be operated by trained and authorized personnel.
● The repair of the equipment can only be done by BIOBASE or BIOBASE authorized dealers.
● If the operator encounters a problem not mentioned in this manual, please contact BIOBASE or
BIOBASE authorized dealers to inquire about the correct handling method.
● The machine must be inspected and maintained within the specified time.
After reading the manual, please keep this manual in a convenient place for easy reference at any
time.

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 Attention
When operating, maintaining, repairing and transporting this instrument, the following basic safety
measures must be strictly observed. If you do not follow these measures or the warnings pointed out
elsewhere in this manual, it may affect the safety protection provided by this instrument. At the same
time, this will also undermine the safety standards of design and manufacturing and the expected use
range of the instrument.

A. Symbol
Attention ： The prompt message requires the operator to confirm this step before
proceeding to the next step. Otherwise, the instrument function will be affected.

Note: The project contains particularly important information, please read it carefully. If you do
not follow the prompts, the instrument may not work properly.。

Warning: A warning message requires you to be especially careful about a certain operation step
or method. If you do not follow the requirements correctly, it may make the instrument unable to
work normally or cause injury to the operator.

Prohibition: The operator is forbidden to perform this operation, otherwise it will cause
damage to the instrument, failure to work normally, or injury to the operator.

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B. Important safety operation information
Before using the nucleic acid extraction instrument, the user must have a complete understanding of
the use of the instrument. Before operating the system, please read this manual carefully.
Note: If the user does not follow the instructions on the manual for all consequences caused
by the operation, our company will not bear any responsibility.

Note: When using this instrument for the first time, please remove the Pearl cotton fixed on
the stirring sleeve according to the instructions attached to the handle of the safety door.
Otherwise, our company will not take any responsibility for the damage to the instrument.

Note: After unpacking the instrument, check the contents of the packing box according to the
packing list. If the item is damaged or missing, please contact our company or supplier. After
passing the acceptance, please fill in the relevant content on the installation information
feedback form and feed it back to our company for documentation and warranty. After
unpacking the instrument, please properly save the packaging materials for future use. Our
company will not bear any responsibility for the damage of the instrument due to improper
packaging while being sent to the maintenance department.

Note: Under the following conditions, immediately unplug the power plug of the instrument from the
power socket, and contact the supplier or ask our professional maintenance personnel to deal with it:
 Theinstrumenthas beenexposedtorainor water;
 Abnormalsoundorsmelloccurswhen theinstrumentis working;
 Theinstrumentisdroppedorthecasingisdamaged;
 Theinstrumentfunctionhaschangedobviously.

Warning:
1. Do not open the door during operation;
2. During operation, the surface of the heating strip may be hot, please do not touch it to avoid
occurrence scald.
Warning: The operator is not allowed to open the instrument, replace the components or debug the
instrument without authorization from our company. If you need to open the instrument, it must be
completed by a professional with the consent of our company, otherwise it will not be guaranteed.

Warning: This instrument has voltage that is harmful to the human body. Please cut off the power of the
instrument at any time before opening the case.

Prohibition:
1. It is forbidden to clean the instrument while the instrument is working;
2. It is forbidden to use organic solvents such as concentrated alcohol to clean the surface of the
instrument.
Prohibition: It is strictly forbidden to use the handle on the safety door of the experiment cabin to drag the
instrument when moving the instrument

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 Content
Preface.................................................................................................................................................... 1
Attention.................................................................................................................................................2
1. Overview............................................................................................................................................ 6
1.1 Product Description...................................................................................................................6
1.2 Experiment Cabin..................................................................................................................... 7
2. Working principle...............................................................................................................................8
3. Application......................................................................................................................................... 9
4. Working environment.........................................................................................................................9
5. Technical parameter........................................................................................................................... 9
6. Performance characteristics..............................................................................................................10
7. The main structure............................................................................................................................10
8. Installation and debugging............................................................................................................... 10
9. Preparation for use............................................................................................................................11
10. Operation........................................................................................................................................ 12
10.1 Boot.......................................................................................................................................12
10.2 Program Edit......................................................................................................................... 13
10.2.1 New program.............................................................................................................. 13
10.2.2 Program content editing..............................................................................................14
10.3 Program Running.................................................................................................................. 16
10.3.1 Program selection....................................................................................................... 16
10.3.2 Program running......................................................................................................... 16
10.4 UV settings............................................................................................................................17
11. Troubleshooting..............................................................................................................................19
12. Maintenance................................................................................................................................... 20
13. Label description............................................................................................................................ 21
14. Contraindications............................................................................................................................22
15. Store................................................................................................................................................22
16. Packing List....................................................................................................................................22

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17. Warranty commitment....................................................................................................................23
Appendix: Experimental Process......................................................................................................... 24

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 1. Overview

1.1 Product Description

Display

Safety door

Figure 1-1 The front of the nucleic acid extractor

USB connector

Power
connecto
r
RS232
Power
switch

Figure 1-2 The back of the nucleic acid extractor

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1.2 Experiment Cabin

Figure 1-3 Experiment Cabin

As shown in Figure 1-3, the contents of the experiment cabin are as follows:
(1) Magnetic rod holder and magnetic rod
(2) Mixing frame
(3) Guide strips at both ends of the 96-well deep-well plate
(4) Heating strip
(5) Middle guide strip of 96-well deep well plate

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 2. Working principle
This instrument is a high-tech product that uses the universal magnetic bead method to extract
nucleic acids. It has the advantages of high automation, fast extraction speed, stable results, and easy
operation. Using a dedicated 96-well deep well plate, 1-48 samples can be operated simultaneously.
Use the magnetic rod on the magnetic rod holder of the experiment chamber to move the magnetic
beads with nucleic acid to different reagent wells, and then use the stirring sleeve sheathed on the
outer layer of the magnetic rod to stir the liquid repeatedly and quickly to make the liquid and the
magnetic beads uniform After cell lysis, nucleic acid adsorption, washing and elution, high purity
nucleic acid is finally obtained, as shown in Figure 2-1.

Figure 2-1 Schematic diagram of the principle of nucleic acid extraction

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 3. Application
It is widely used in routine scientific research, genomics, disease control system, food safety,
forensic and other fields. To use this instrument, you only need to add the sample and the automatic
nucleic acid extraction reagent with magnetic beads as the carrier to the 96-well deep-well plate,
select or edit the appropriate program and execute it. With different types of magnetic bead nucleic
acid extraction reagents, it can quickly extract DNA and RNA in samples of animal and plant tissues,
blood, body fluids, and criminal materials.

4. Working environment
a) Ambient temperature: 10 ℃～30 ℃;
b) Relative humidity: ≤70%;
c) Atmospheric pressure: 86 kPa～106 kPa;
d) There is no strong vibration and corrosive gas around;
e) Avoid direct sunlight or other heat and cold sources;
f) Keep away from strong electromagnetic field interference;
g) Power supply voltage: 110-220 V, 50 Hz、60Hz;

5. Technical parameter

Model BNP48
Sample number 48
Magnetic rod (fixed) 6*8
Sample volume 60~1000ul
Collection efficiency of magnetic beads >98%
Magnetic beads size 0.2~1.0um

Size（mm） 560*385*490（L*W*H）

Service life 8 years

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 6. Performance characteristics
1) Sample protection: The instrument has functions such as power-on self-check and power-off
protection to minimize the possibility of sample loss during the use of the instrument;
2) Modular design: The modular structure is adopted, and the core components are all independently
designed, which has higher efficiency and lower failure rate, ensuring better stability during the
operation of the instrument;
3) Humanized interface: program visualization, precise control, simple operation, easy to use;
4) Independent programming control: According to user needs, the program can be freely edited;
5) Open platform: suitable for a variety of nucleic acid methods based on bio-nanomagnetic beads.

7. The main structure

The nucleic acid extractor is mainly composed of heating module components, magnetic separation
module components, industrial control module components, base frame module components, and
housing components.

8. Installation and debugging

1) Place the instrument
This instrument is only suitable for indoor installation with good indoor ventilation, no corrosive gas,
and no strong magnetic field interference. Do not place the instrument in a humid or dusty place; do
not place the instrument on a soft cushion, so as to prevent the base from sinking into the soft
cushion and block the vent below; the openings on the instrument are for ventilation and heat
dissipation. Leaning against walls or stacking other objects, do not block or cover these ventilation
holes. When the instrument is running, the distance between the ventilation holes of the instrument
and the nearest object should not be less than 25cm, so as not to affect heat dissipation; do not use
the instrument in direct sunlight. Keep away from heaters, stoves and all other heat sources.
2) Ground the instrument
To avoid electric shock, the input power cord of the instrument must be grounded reliably. This
instrument uses a three-pin plug, which can only be used with this type of power socket. This is a
safety device.
3) Power supply
a. Power requirements
Before connecting the AC power supply, make sure that the voltage of the power supply is consistent
with the voltage required by the instrument (a deviation of ±10% is allowed). And make sure that the
rated load of the power socket is not less than the requirement of the instrument.
b. Pay attention to the power cord
This instrument should usually use the power cord that comes with it. If the power cord is damaged,
it must be replaced and not repaired. The power cord of the same type and specification must be used
instead. When the instrument is in use, do not put anything on the power cord, and do not place the
power cord where people move.
c. Plug and unplug the power cord
Always hold the plug when plugging or unplugging the power cord. When inserting the plug, make
sure that the plug is fully inserted into the socket. Do not pull the power cord when unplugging the
plug.

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 9. Preparation for use
After the instrument is installed, you must first remove the Phillips screw fixed on the stirring sleeve
according to the prompt information of the front safety door, then turn on the power, close the front
door, and then power on.
Warning: When the instrument is used for the first time, the cross screws fixed on the stirring sleeve
must be removed.
Note: If the experiment or reagent requires high temperature, please start the program with
heating (heating temperature is set to 60℃), preheat for 30 minutes, and then perform the
experiment.

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 10.Operation
10.1 Boot
Turn on the nucleic acid extraction instrument switch, the buzzer emits two short beeps, and the
instrument is starting. After the instrument enters the system, it starts to initialize the equipment,
reset and self-check of the motion device. This process takes about 1 to 2 minutes. The system
initialization interface is as follows As shown in Figure 3-1.

Note: When the instrument is turned on for the first time, please follow the instructions attached
to the handle of the safety door and remove the two Phillips screws that are fixed on the stirring
sleeve. Otherwise, the instrument will be damaged when it is powered on and the instrument will not
work properly .

Figure 10-1 Instrument initialization interface

After the instrument is powered on, the equipment is initialized, the motion device is reset, and the
self-check is over, the system enters the main operation interface, as shown in Figure 10-2.
The main operation interface includes shortcuts for instrument functions and program files. The
upper one is the system function keys, including "Program running", "Program edit", "Setup", and
"UV disinfection".
Click the experiment name or the icon above the name to enter the running interface of the program
file.

Note: When powering on, please close the front safety door so that the instrument can initialize,
reset and self-check normally, and enter the main operation interface.

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 Figure 10-2 Main operation interface
10.2 Program Edit
Click "Program Edit" in the main operation interface to enter the program editing interface directly.
As shown in Figure 10-3.

Figure 10-3 Program edit interface

10.2.1 New program
After selecting one of the rows and the color overall turns green, touch "Edit", a new program
naming window will pop up, as shown in Figure 10-4.

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 Figure 10-4 New program naming window
Touch the box below the experiment name, enter the name of the program, and touch Enter to
confirm. To cancel the creation, touch the Delete key to clear the information, and then touch the
Enter key to return.
10.2.2 Program content editing
Select the newly created program or the program that has been edited in the previous stage, touch
"Edit" in the program editing interface to enter the program editing interface, as shown in Figure
10-5.

Figure 10-5 Program edit window

Program editing needs to start from the first row, select the first row, and after the color turns red as a
whole, touch "Edit" to enter the single program editing interface, as shown in Figure 10-6.

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 Figure 10-6 Single program edit window
Position: the hole position on the 96-well deep-well plate corresponding to this step.
Names: Lysis (lysis), Bead (magnetic bead recovery), Bind (magnetic bead preparation), Wash
(washing), Elution (elution).
Waiting time: the waiting time before the magnet bar enters the liquid.
Mixing time: the mixing time of this step.
Magnetic suction time: the time for magnetic bead adsorption in this step.
Mixing speed: fast, medium and slow
Volume: The volume of solution added in the hole.
Temperature setting: click on temperature to enter the temperature setting interface, as shown in
Figure 10-7.

Figure 10-7 Single program edit window

Lysis temperature: the appropriate temperature required for cracking.
Elution temperature: the appropriate temperature required for elution.

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10.3 Program Running
The program running includes all the experimental program files stored in the system. The user can
select the appropriate experimental program file and run it according to his own requirements for the
experiment.
10.3.1 Program selection
Touch "Program Running" on the main operation interface to enter the interface for selecting a
program to run, as shown in the figure 10-8.

Figure 10-8 Program selection Interface

Enter the next page of the program, if the last page is displayed, it will not response if you
touch this button.

Enter the next page of the program, if the first page is displayed, it will not response if you
touch this button.
Return to the previous operation interface.

Enter the interface of the selected program. Before touching this button, you need to select
the program to run. If the program is not selected, touching this button will have no response.
10.3.2 Program running
Select the program and touch "OK" to enter the program running interface, as shown in Figure 10-9.

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 Figure 10-9 Program running interface
10.4 UV settings
Touch " UV disinfection " in the main operation interface, and the UV lamp running time setting will
pop up, as shown in Figure 10-10.

Figure 10-10 UV lamp setting interface

Set the corresponding UV lamp running time as required. Click "No" on the interface of Figure 10-9
to exit this interface and the UV lamp is not turned on. Click "Yes", the UV lamp is turned on, and
the UV lamp running time will pop up, as shown in Figure 10-11.

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 Figure 10-11 UV lamp running interface
Touch "Close" to turn off the UV lamp.

Note:
1. During the disinfection process of the UV lamp, please do not open the front safety door. If the
experimenter hits the safety door at this time, the UV lamp will automatically turn off and the time
will stop counting down. After closing the safety door, the UV lamp will be turned on again and the
time will continue to count down until the end of the timer.
2. When the disinfection time is up, the system will automatically turn off the UV lamp, or the
experimenter will turn off the UV lamp according to the actual situation;
3. The plexiglass of the front safety door has the function of filtering ultraviolet light. To avoid
accidents, please do not look directly at the ultraviolet light;
4. During the UV lamp disinfection process, except for the UV lamp icon, other icons continue to be
available, but the program cannot be run, and the program can only be run when the UV lamp is
turned off.

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 11.Troubleshooting
No. Question Reason Solution

Power cable connection is notReconnect the power cable

well
The screen does not
display after turningThe fuse is damage Replace the fuse
1
on the power
Whether the switch indicatorContact our company or
light is on supplier
Others

Whether the screw fixing theRemove the fixed screws

mixing sleeve rack is removed

2 The machine makes Whether the mixing sleeve isReinsert the mixing sleeve
an abnormal sound fully inserted

Whether the 96-well deep wellReplace the deep-well plate

plate is placed correctly

Check the temperature control Open the temperature

control

3 The temperature hasother Contact our company or

not risen supplier

4 Uv lamp is not The UV lamp is not connectionReinstall the UV lamp

working well
UV lamp is damaged Replace the UV lamp
No movement Pause the program, touch
the "Reset" button to reset

5 Abnormal magneticOthers Contact our company or

bar action supplier

6 Magnetic rod falls Contact our company or

off supplier

Improper use or operation error Turn off the power and

restart the instrument
Others Contact our company or
7 System freezes or
supplier
loses control

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 12.Maintenance
In order to enable the nucleic acid extractor to work long-term, safely and effectively, and to extend
its trouble-free working time, the machine should be regularly maintained and inspected. If you have
any questions or problems, please notify the management and the company's maintenance
department immediately.
1) Please check the manual carefully before using the instrument;
2) After the experiment is over, turn off the power supply of the instrument and use 75% ethanol to
clean the experiment chamber. When using it, do not pour ethanol into the experiment chamber.
Please wipe with absorbent cotton. After the ethanol is dry, turn on the UV lamp. Irradiate for more
than 30 minutes for disinfection;
3) Clean the surface of the instrument and the experimental chamber regularly, and avoid using
strong alkali, concentrated alcohol and organic solvent solutions;
4) Keep the environment in the experiment cabin relatively dry and free of water stains and other
things;
5) Do not use the instrument in a dusty environment;
6) When using the instrument, please ensure ventilation around the instrument;
7) Please do not use the instrument when the voltage is unstable, too high or too low;
8) When the instrument is not in use for a long time, please unplug the plug and cover the instrument
with a soft cloth or plastic bag to prevent dust from entering.
9) When the instrument is out of use, in order to ensure the stable performance of the instrument, it is
recommended to start the instrument to run without sample once every 30 days.

Prohibition: It is forbidden to clean the experiment chamber or the surface of the

instrument while the instrument is working.

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 13.Label description
1）Logo

2）Biological hazard

3）Pay attention to high temperature

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 14.Contraindications
Nucleic acid extractor currently has no known contraindications.

15.Store
The packed nucleic acid extractor should be stored at 10℃～30℃, relative humidity not exceeding
70%, no corrosive gas and well ventilated environment.
The storage period of the nucleic acid extractor should not exceed one year, and the nucleic acid
extractor that exceeds one year should be checked out of the box, and the nucleic acid extractor that
passes the open box inspection can enter the circulation field.

16. Packing List

BNP48 Packing List
Name Amount
BNP48 main instrument 1
Power cable 1
BNP48 User manual 1
Test report 1
Certification 1
Warranty card 1
Product acceptance form and installation report 1

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 17.Warranty commitment
Dear customer: Any product has the possibility of failure. Please monitor the operating status of the
equipment in real time during use. If there is any abnormality, please refer to the manual to deal with
it. If it still cannot be solved, you should notify the company's service center in time to avoid in order
to avoid your losses..
After-sales service matters
1. The whole machine is guaranteed for one year free of charge from the date of equipment sale, and
technical support is provided.
2. Warranty certificate: When you need normal consultation or maintenance, please contact our
company's local after-sales service center with the warranty certificate and purchase invoice and
keep the warranty certificate properly.

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 Appendix: Experimental Process

1.The main content

The purpose of this manual is to enable users to combine the operation of the BNP48 nucleic acid
extractor with the reagents used, so as to master the operation process of this instrument as soon as
possible.
2.Operation
Before performing nucleic acid extraction, please select the magnetic bead method nucleic acid extraction reagents
that are compatible with the BNP48 nucleic acid extractor. The general nucleic acid extraction reagents are divided
into: lysis solution, magnetic bead solution, washing solution I, washing solution II and eluent, etc. ingredient.

The role of each component:

Component Role

Lysis solution Lyse the sample and release the nucleic acid

Magnetic bead
Adsorb the nucleic acid released from the sample
solution

Washing solution Wash away other substances other than nucleic acids such
I/ II as protein

Eluent Elution of nucleic acid adsorbed on magnetic beads

The composition and concentration of each component vary according to the classification of the
sample to be extracted. Please strictly select the appropriate nucleic acid extraction reagent according
to the classification of the sample.

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I. Take BIOBASE Nucleic acid extraction kit 4 (magnetic bead method) as an
example to introduce the DNA/RNA extraction operation:
1.1. Material:
Tips and centrifuge tubes without nuclease contamination
1.2. Preparation before use:
a) Take out the pre-packaged reagent plate from the kit and invert several times to resuspend the
magnetic beads evenly.
b) The pre-dispensing plate should be shaken lightly or quickly centrifuged before use to avoid
reagent staining. Carefully tear off the aluminum foil sealing film to prevent liquid from spilling
1.3. Sample type:
Blood, animal tissue, environmental samples, saliva, nasal fluid, swabs, etc.
1.4. The composition of the kit (32T):

Composition Specification （32T）

Pre-packaged reagent plate 3*16 tests

Lysis solution 2 700ul
Magnetic rod sleeve 3*2

1.5. Operation Steps：

a) Take 200ul of the processed sample and 20ul of Lysis Solution 2 and add them to wells A1~H1 or A7~H7 of
the pre-packing plate;
b) Put the 96-well deep-well plate into the BNP48 nucleic acid extractor, and confirm that the gap of the 96-well
deep-well plate is facing inward.
c) Insert the Magnetic rod sleeve into the stirring frame.
d) Close the safety door of the experimental cabin of the BNP48nucleic acid extractor.
Lysis Elution
Waiting Mixing Magnetic Mixing Volume
No. Position Name temp. temp.
time（s) time（s) Suction time（s）speed （ul)
(℃） （℃）
1 4 Bind 0 0：30 0：30 F 200 0 0
2 1 Lysis 0 4：00 0：30 F 800 80 0
3 2 Wash 0 0：30 0：30 F 800 0 0
4 3 Wash 0 1：00 0：30 F 800 0 0
5 5 Elution 0 3：00 0：30 S 100 0 80
6 3 Bead 0 0：30 0：0 F 800 0 0

e) Prepare the instrument operating program according to the following operating steps

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f) Turn on the nucleic acid extractor, enter the program editing interface, fill in the program
parameters listed in the table in e) one by one, and then touch Save.
g) Touch "Program Running" on the main interface to enter the program running interface. After selecting the
program you want to run, touch OK to enter the selected program interface, as shown in Figure 1. Tap "Run"
to run this program (for details, refer to the 10.2 "Program Editing" section of the manual).

Figure 1 Program running interface

h) After the program runs, the extraction results are stored in the 5/11hole position, and the liquid
in the 5/11hole position is sucked out and placed in an EP tube and stored at -20°

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II. Experimental process and Attention

1. When using the instrument for the first time, be sure to open the door first, remove the two cross
fixing screws on both sides of the stirring frame in the experiment chamber, and then power on for
the test.
2. Check whether the instrument and power supply are intact, and then turn on the power after
confirming that they are intact;
3. Place samples and reagents in the corresponding wells of the 96-well deep-well plate according to
experimental requirements;
4. Put the 96-well deep-well plate into the experiment chamber, push the stirring sleeve into place,
and carefully check whether it is in place. If it is not checked, it may cause instrument abnormalities
and affect the experimental results;
Tip: 96-well deep-well plate notch facing inward and placed in place
Tip: The mixing sleeve has been pushed into place
5. Close the safety door of the experiment cabin, edit the settings or directly select the program file to
be run, and press start;
6. During the running of the program, if there are no abnormalities or experimental needs, the
experimenter should not open the actual
If the inspection safety door needs to be opened, please suspend the operation procedure;
7. After the normal operation of a program file is completed, the buzzer emits intermittent long beeps
to indicate the end of the experiment.

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BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: [email protected]
Complaints: [email protected]
After-sales service: [email protected]; [email protected]
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com