Course Documentation Outline

School of Business, Biosciences and Justice Studies

SECTION I

1. Program (s): Biofood, Biotechnology, Chemical, Environmental

2. Course Name: Instrumentation 1 Lab

3. Course Code: CHEM 2006

4. Credit Value: 3 Course Hours: 45

Class Lab Field Other Total

45 45

5. Prerequisites/Co-requisites/Equivalent Courses

PR/CO/EQ Course Code Title

PR CHEM1003 General Chemistry 2
CO CHEM 2001 Instrumentation 1 Theory

6. Faculty: Elinor Brunet Date: Sept 17, 2008 Effective Date: Sept 2, 2008

7. Dean Approval: Dan Holland Date: August 2008

9. Revision Number: Date: Effective Date:

10: Notes

1
Section II

11. Calendar Description:

The labs performed in this course support the theory studied in CHEM2001 Chemical
Instrumentation I Theory.
.

12. Provincial Context:

This course meets the following Ministry of Education and Training requirements:

a). Prior Learning Assessment (PLA)

Students may apply to receive credit by demonstrating achievement of the course
learning outcomes through previous life and work experiences.

This course is eligible for challenge through the following method(s) indicated by *

Challenge Exam Portfolio Interview Other Not Eligible

* * *

PLAR Contact:

13. Employability Skills emphasized in this course

communication - written communication - visual communication - oral

* analytical creative thinking * decision making
* interpersonal * numeracy * organizational
* problem solving * technological other (specify)

14. Required Texts, Materials, Resources or Technical Materials Required:

Lab manual produced at the college, lab coat and safety eyewear (CSA approved) with colourless lenses,
as well as a scientific calculator capable of linear regression. A formal textbook is not required for this
course.

15. Evaluation Plan

Students will demonstrate learning in the following ways:

Assignment Description Evaluation Methodology Due Date

Lab Reports 90% On going
Personal Assessment 10% On going
16. Other

• All labs must be performed and the data recorded and initialled before leaving the lab (penalty -10%).
• A lab report must be submitted for each lab, one week after the lab is completed.
• Students will work with a partner to perform the labs but must each submit their own personalized,
individual lab report (the penalty for duplicate reports is –50%)
• All labs will be returned in the final week of the semester (before the final test).
• There will be prearranged make up periods during the semester: students may perform only one
make up lab.
• There may be a final evaluation test that may take the form of a practical exam at the end of the
semester.
• The 10% assessment mark represents an evaluation of how well the students learn the lab
techniques demonstrated, how safely the students work, the cleanliness of their workstation.

Contact Information for Elinor Brunet:

Office: 2L25 a
Work Phone #: 613-969-1913, ext 2290
Home Phone #: 613-968-8695
E-mail: [email protected]

3
Section III

17. Curriculum Delivery, Learning Plan and Learning Outcomes:

Course Related Learning Outcomes Learning

Components/Content Activities/Resources
Spectroscopy The student will be able to: Lab:
- prepare a schematic Operation and
Spec 20 Response of Spec
- find, name and explain the purpose of
the main parts of a Spec 20
- recognize that the detector and lamp
respond differently at varying wavelengths
of energy and use this to determine the
wavelength at which this instrument is
most responsive
- relate the colour of light to its wavelength
Spectroscopy - use a double beam spectrophotometer Lab:
to scan the wavelengths to determine λmax Visible Spectrometry
Spec 21 and Perkin Elmer and then relate that to the observed
Hitachi 200 colour of the solution
- use a single beam spectrometer to
determine λmax of a solution
- to set up and operate both a single
beam and double beam instrument

Perkin-Elmer Hitachi 200 and - use a double beam spectrophotometer

Genesys 10UV Thermo to scan the wavelengths to determine λmax Lab:
Spectronic
- scan a series of standards that increase Quantitative Spec.
in concentration to observe the increase in
peak height at λmax then convert that peak
height to absorbance
- measure the absorbance of the same
series of standards on the single beam
instrument
- perform linear regression on both sets of
absorbance vs. concentration data
- the concentration of an unknown is
determined from the data
Atomic Absorption - be able to start up, calibrate and obtain Lab:
absorbance readings of metal standards Furnace and Flame AA
Varian Flame AA on both instruments
Varian Furnace AA
Polarimeter and - prepare dextrose standards by % w/w Lab:
Refractometer concentration
Polarimeter and
- operate the polarimeter to measure the refractometer
observed rotation by the dextrose
standards
- calculate the specific rotation of dextrose
- measure the rotation of an unknown
dextrose solution and calculate the
concentration (% w/w) of dextrose in the
unknown
- measure the % w/v sugar present in the
standards and maple syrup standards on
a refractometer
Chromatography - set up the developing tank Lab:
Thin Layer and Preparative - prepare a TLC plate (apply mixture) Thin Layer 1
Column
- develop and visualize the TLC plate Thin Layer 2
-calculate the Rf value for the Column
components, the mobile and stationary Chromatography
phases affect of the Rf value and the
resolution of the components
-separate a mixture of compounds on a
silica gel column, collect the fractions as
they come off with a fraction collector, and
prepare a TLC plate to identify the
compound(s) present in each fraction by
comparing their Rf value to the results that
they obtained in the TLC 2 lab
Gas Chromatography - point out the parts and describe their Lab: GC 1
purpose
Shimadzu GC8A - start up, run chromatograms and shut
down the GC
- introduce reproducible injections
- predict, knowing the boiling points, the
order of elution of a mixture of compounds
- be able to describe the effects of
changing column temperature, and
attenuation on retention time and peak
height
- be able to calculate retention time from
the recorder output
- be able to measure the gas flow rate and
install a column

5
Fluoride Determination -accurately prepare standards that contain Lab: Fluoride
fluoride by performing a serial dilution
from a fluoride stock that was supplied
- set up a potentiometer to which a
combination fluoride electrode has been
connected
- obtain a millivolt reading for each of the
fluoride standards and an unknown
-plot the potential readings for the
standards on semi-log graph paper and
interpolate the reading obtained for the
unknown so that the concentration of the
unknown can be stated
Liquid Chromatography - be able to identify the parts and explain Lab: HPLC 1
their purpose
Shimadzu LC - be able to start up, introduce samples,
obtain chromatograms and shut down the
instrument
- appreciate the difference in
reproducibility between student controlled
injections and injections controlled by the
sample loop
- describe the effect on peak height of
changing the absorbance setting
- describe the relationship between pump
pressure and mobile phase flow rate

- understand the effect of recorder speed

on peak width and height
Upon successful completion of this course, the student will be able to:
a) Prepare standards and samples accurately by dilution or dissolution
b) Prepare, develop, visualize, and interpret thin layer chromatography plates of various polarity
samples in various solvents and calculate Rf values for the components of the samples.
c) Perform the following tasks on these instruments: GC (gas chromatography), HPLC (high
performance liquid chromatography), Flame and Furnace Atomic Absorption (AA), polarimeter and
refractometer, potentiometer set up for a fluoride determination and spectrophotometer
- Identify the parts of the instrument and state their purpose
- Trace the path of the light or mobile phase
- Set-up and calibration
- Introduction of standards and samples
- Manipulation and evaluation of the qualitative and quantitative data obtained by using graphing
and linear regression
- Expression of results of all calculations to the appropriate number of significant figures or decimal
places with the appropriate units
d) Practise relevant safety procedures while performing lab exercises
e) Use appropriate reference materials (Merck Index, CRC Handbook) to obtain required information
f) Install and use the regulator on a gas cylinder safely