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LAB - Temperature Effect On Enzymes - SBA AI

The lab experiment investigates the effect of temperature on enzyme activity using potato catalase and hydrogen peroxide. The procedure involves measuring the height of foam produced at various temperatures after adding potato pieces to hydrogen peroxide. Results are analyzed to understand the relationship between temperature and enzyme reaction rates, with discussions on methodology, limitations, and potential improvements.

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23 views3 pages

LAB - Temperature Effect On Enzymes - SBA AI

The lab experiment investigates the effect of temperature on enzyme activity using potato catalase and hydrogen peroxide. The procedure involves measuring the height of foam produced at various temperatures after adding potato pieces to hydrogen peroxide. Results are analyzed to understand the relationship between temperature and enzyme reaction rates, with discussions on methodology, limitations, and potential improvements.

Uploaded by

Kk
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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LAB ON ENZYMES – TEMPERATURE EFFECT SKILL: AI (SBA)

SYLLABUS OBJECTIVE: Module 1 Obj. 4.3 – To explain the effects of pH, temperature,
enzyme concentration and substrate concentration on enzyme action.

LAB INFORMATION

DATE:

TITLE:

AIM:

APPARATUS: Stopwatch, test tubes, water baths (0oC, 20oC, 40oC, 60oC, 80oC and
100oC), cork borer, knife, white tile, ruler, soap detergent, 1.0M Hydrogen
Peroxide, potato

METHOD:
1. Use the cork borer to obtain potato cylinders. Cut potato pieces 2mm in length.
2. Incubate six 10 mL test tubes containing 10 mls hydrogen peroxide at different
temperatures: 0oC, 20oC, 40oC, 60oC, 80oC and 100oC for five minutes.
3. Add one drop of soap detergent to each tube.
4. Add 3 cubes of potato to the six test tubes.
5. Measure the height of foam after 1 minute.
6. Carry out the procedure out three times at each temperature and calculate the average.
7. Tabulate the results and record what is observed as temperature is increased.
8. A graph of Height of Foam (mm) against Temperature (oC) was drawn.
RESULTS: Table showing the Height of Foam obtained at different temperatures

Temperature Height of Foam (mm) Average Height


(oC) of foam (mm)
1 2 3
0
20
40
60
80
100

Graph of Average Height of Foam (mm) against Temperature

Discussion: Guiding questions


1. Explain the theory of the reaction between occurring between the potato and the hydrogen
peroxide.
2. Explain the concept of the effect of temperature on the rate of enzyme reaction.
3. Describe the trends observed in the results obtained.
4. Explain the results using the theory.
5. State the limitations, precautions and reliability of the procedure.
6. State 3 ways in which the method can be improved.
7. What conclusion can be made from this experiment?

Conclusion:
MS for Lab on Effect of Temperature on Enzyme Activity.

Criteria for Assessment Maximum Mark Student Mark

ANALYSIS &INTERPRETATION
A: Analysing evidence/data and drawing
Conclusions.
 Background information on principle on
which experiment is based –
*Reaction between catalase and hydrogen
peroxide 1
*ref. to bubble formation 1

 Underlying concepts {effect of


temperature on enzyme activity} 1

 Describe trend of results 1

 Explanation of results of different


temperatures 1

 Making conclusions based on data:

Effect of temperature on
enzyme activity. 1

B Evaluation of procedure
 Discussion of validity of method/limitations
of method. 1

 Reliability of method: repeated and


1
averages taken

 Precautions for valid results: same


1
volume of solutions used etc.

 Improvement of method: 1
*include a control 1
*maintain constant water bath temperature 1
*use pureed potato
Total 12

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