1. The enzyme responsible for linking new DNA nucleotides to 5.

5. During replication DNA ___ fills the gaps between the

the growing DNA strand during DNA replication is called: Okazaki fragments in the lagging strand by forming ___
a. DNA Replicase bonds between the neighboring nucleotides.
b. DNA Ligase a. Ligase; covalent
c. DNA Helicase b. Ligase; hydrogen
d. DNA Polymerase c. Polymerase; covalent
2. If the template strand on a region of DNA to be copied d. Polymerase; hydrogen
reads: AGCTTGCA, the complementary DNA strand will 6. If you were given the following DNA sequence 5' T G A A T C
read: G A T C A T A T 3' what would be the correctly read,
a. TGCAAGGT corresponding mRNA sequence?
b. UCGAACGU a. 5’ A C T T A C G T A G T A T A 3’
c. UGCAAGGU b. 5’ A T A T G A T G C A T T C A 3’
d. TCGAACGT c. 5’ A C U U A G C U A G U A U A 3’
3. During DNA replication, a template strand of ATCGTA will d. 5’ A U A U G A U C G A U U C A 3’
bond to a strand with the nucleotide sequence 7. In prokaryotes, transcription occurs in the ________ and
a. UAGCAT translation occurs in the_________. In eukaryotes,
b. TAGCAT transcription occurs in the ________ and translation occurs
c. ATCGTA in the_________.
d. AUCGUA a. cytoplasm, ribosome, cytoplasm, ribosome
4. Because of base pairing in DNA, the percentage of b. nucleus, cytoplasm, nucleus, cytoplasm
a. Adenine molecules in DNA is about equal to the c. cytoplasm, cytoplasm, nucleus, cytoplasm
percentage of guanine molecules. d. nucleus, ribosomes, nucleus, ribosome
b. Thymine molecules in DNA is about equal to the 8. DNA replication results in two DNA molecules, __________.
percentage of adenine molecules. a. each with two new strands
c. Adenine molecules in DNA is much greater than the b. each with two original strands
percentage of thymine molecules. c. each with one new strand and one original strand
d. Cytosine molecules in DNA is much greater than the d. one with two new strands and the other with two
percentage of guanine molecules. original strands
9. The two strands of DNA are connected by 14. What structural problem prevents adenine from pairing
________________ bonds between the ________________. with guanine?
a. covalent; phosphate groups a. The bases are both short.
b. covalent; nitrogenous bases b. They lack phosphate groups.
c. hydrogen; phosphate groups c. They lack the deoxyribose group.
d. hydrogen; nitrogenous bases d. The bases are both long.
10. Choose the appropriate complementary DNA base pair. 15. What is the complementary DNA sequence for the following
a. A-G strand of DNA: TACACCTTGGCGACGACT?
b. A-C a. TCAGCAGCGGTTCCACAT
c. A-T b. ATGTGGAACCGCTGCTGA
d. A-U c. AUGUGGAACCGCUGCUGA
11. During DNA replication, a cell is unable to add new d. TACACCTTGGCGACGACT
nucleotides to the template strand in order to synthesize a 16. Which of the following statements is true?
new strand. Which of the following enzymes is most likely a. DNA can be proofread during copying.
being inhibited? b. Only RNA can be proofread during copying.
a. RNA polymerase c. DNA can only be proofread during prophase.
b. DNA polymerase d. DNA can only be proofread during metaphase.
c. Nuclease 17. During replication, the function of the enzyme DNA
d. Helicase polymerase is to
12. DNA is being copied. The template strand is ACTGCA. What a. unzip the DNA.
is the new complementary strand of DNA? b. produce mRNA.
a. UGACGU c. bind free-floating nucleotides to the parent DNA
b. TGACGT strand.
c. TGCGTA d. wrap the DNA back up.
d. UGAGUC 18. In which process is DNA polymerase directly involved?
13. If one strand of a DNA double helix has the sequence a. transcription
GTCCAT, what is the sequence of the other strand? b. translation
a. ACTTGC c. replication
b. TGAACG d. all of the above
c. CAGGTA
d. CAGGUA
19. Which enzyme is responsible for synthesizing a new strand 23. What enzyme separates the two strands of DNA to begin
of DNA? replication?
a. DNA polymerase a. Nuclease
b. Helicase b. DNA polymerase
c. RNA polymerase c. Ligase
d. Protease d. Helicase
20. In which part of the cell does replication occur? 24. After replication, ______.
a. Rough endoplasmic reticulum a. each new DNA double helix consists of two old
b. Golgi complex strands
c. Nucleus b. each new DNA double helix consists of one old
d. Ribosomes strand and one new strand
21. What nucleotide sequence in the DNA tells the cell where to c. each new DNA double helix contains 25% of the old
start copying the DNA? DNA double helix
a. Origins of replication d. one new DNA double helix consists of two old
b. Promoter sequences strands and the other new DNA double helix
c. Start codons consists of two new strands
d. Terminator sequences 25. Which choice below occurs during DNA replication?
22. Which of the following best describes the process of DNA a. Covalent bonds between the sugar and phosphate
replication? group are broken.
a. Helicase unzips DNA molecules, and RNA b. Hydrogen bonds between the nitrogenous bases
polymerase attaches free nucleotides to construct a are broken.
replicate. c. RNA polymerase attaches to the DNA.
b. DNA polymerase unzips the DNA molecule, and d. One strand of the DNA is copied and the other is
helicase uses each strand as a template. destroyed.
c. Helicase unzips the DNA molecule, and RNA 26. Which type of bond holds the nitrogen bases together
polymerase uses each strand as a template to across a DNA double helix?
attach corresponding nucelotides. a. Covalent
d. Helicase unzips the DNA molecule, and DNA b. Metallic
polymerase uses each strand as a template to c. Ionic
attach corresponding nucleotides. d. Hydrogen
27. DNA replication is semiconservative. When one DNA 32. The fragments of DNA are joined together by which of the
molecule is copied to make two DNA molecules, the new following enzymes?
DNA contains ______. a. Endonuclease
a. none of the parent DNA. b. DNA polymerase
b. 50% of the parent DNA. c. Primase
c. 75% of the parent DNA. d. Ligase
d. 100% of the parent DNA
28. What is the origin of replication?
a. Particular site at which DNA replication starts
b. Site which prevents initiation
c. Random location on the DNA
d. Site at which replication terminated
29. Which of the following enzymes separates the two strands
of DNA during replication?
a. Gyrase
b. Topoisomerase
c. Helicase
d. DNA polymerase
30. Which of the following enzymes remove supercoiling in
replicating DNA ahead of the replication fork?
a. DNA polymerases
b. Helicases
c. Primases
d. Topoisomerases
31. DNA polymerase synthesizes__________
a. DNA in 5’-3’ direction
b. DNA in 3’-5’ direction
c. mRNA in 3’-5’ direction
d. mRNA in 5’-3’ direction