ntended Use: Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious

microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. The broth medium which contains 6.5% sodium chloride is used to differentiate the enterococci from nonenterococcal group D streptococci.

Product Summary: BHI Broth is used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts and molds.1 BHI with 6.5% Sodium Chloride is used to differentiate the enterococci (e.g., E. faecalis, E. faecium, E. durans and E. avium) from the nonenterococcal species (S. gallolyticus and S. equinus) by the 6.5% salt tolerance test.2

User Quality Control: See Quality Control Procedures. Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratorys standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

Reagents: Brain Heart Infusion Approximate Formula* Per Liter Purified Water Brain Heart, Infusion from (solids) Peptic Digest of Animal Tissue Sodium Chloride Dextrose Pancreatic Digest of Gelatin Disodium Phosphate *Adjusted and/or supplemented as required to meet performance criteria.

6.0 6.0 5.0 3.0 14.5 2.5

g g g g g g

Brain Heart Infusion with 6.5% Sodium Chloride contains 60 g/L of sodium chloride in addition to the ingredients listed above.

Warnings and Precautions: For in vitro Diagnostic Use. Caution should be exercised in reporting direct Gram stain and/or other direct microbiological stain results on tissue specimens processed with this medium due to the possible presence of nonviable organisms in the culture medium. Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass. Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.

Brain Heart Infusion Broth (BHI)

Brain Heart Infusion Broth (BHI) is an enriched non-selective medium intended for the cultivation of most anaerobic bacteria and other fastidious microorganisms. This medium is used in the inoculum preparation for antimicrobial susceptibility testing, is especially useful as a base for blood cultures and is also used in the broth-disc antimicrobial test procedure as described by Wilkins and Theil. BHI is an enriched non-selective broth medium that is useful in the cultivating of fastidious and nonfastidious microorganisms. This medium will also support the growth of aerobic microorganisms from a variety of clinical and non-clinical specimens. The basal medium is infusion from brains and beef heart and supplemented with vitamin K1 and hemin as growth factors for most anaerobes. This medium is prepared, dispensed, stored and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use. This medium contains resazurin as a redox indicator which turns pink upon significant oxygen exposure. Otak Jantung Infusion Broth (BHI) adalah media yang non-selektif diperkaya dimaksudkan untuk budidaya bakteri anaerob yang paling cerewet dan mikroorganisme lainnya. Media ini digunakan dalam persiapan inokulum untuk pengujian kerentanan antimikroba, ini sangat berguna sebagai dasar untuk kultur darah dan juga digunakan dalam prosedur uji antimikroba kaldu-disk seperti yang dijelaskan oleh Wilkins dan Theil. BHI adalah non-selektif diperkaya kaldu menengah yang berguna dalam menumbuhkan mikroorganisme cerewet dan non-cerewet. Media ini juga akan mendukung pertumbuhan mikroorganisme aerobik dari berbagai spesimen

klinis dan non-klinis. Media basal adalah infus dari otak dan hati sapi dan ditambah dengan vitamin K1 dan hemin sebagai faktor pertumbuhan anaerob paling. Media ini disusun, dibagikan, disimpan dan dikemas di bawah kondisi bebas oksigen untuk mencegah pembentukan produk teroksidasi sebelum digunakan. Media ini berisi resazurin sebagai indikator redoks yang berubah warna menjadi pink pada paparan oksigen yang signifikan.

Anaerobe Systems PRAS Brain Heart Infusion Broth (BHI) AS-872

View the full product insert: BHI Insert.pdf Brain Heart Infusion Broth Products Item # AS-872 Description BHI, 5mL Size Price

10 11.98 Tubes/pack

Formula
Brain Heart Infusion, 37.0 g Calf Brain Infusion, 7.7 g Beef Heart Infusion, 9.8 g Proteose Peptone, 10.0 g Dextrose, 2.0 g Sodium Chloride, 5.0 g Disodium Phosphate, 2.5 g Hemin, .005 g Vitamin K1, .002 g Resazurin, .001 g Yeast Extract, 5.0 g L-Cysteine, 0.5 g Distilled Water, 1000.0 ml

Final pH 7.3 +/- 0.3 at 25 degrees C. Final volume 5.0 ml +/- 0.5.

Precautions
For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic techniques should be observed when using this product. This product is for use only by properly-trained and qualified personnel. Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life

Storage: Upon receipt, store at room temperature (13C - 27C) in original packaging until use. Avoid overheating or freezing. Do not use medium if there are signs of deterioration

(discoloration or evaporation) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.

Shelf Life: 1 year from date of manufacture.

Procedure
Specimen Collection: Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing of specimens. Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use: BHI should be inoculated directly with clinical material. Inoculated tubes should o immediately placed in an anaerobic atmosphere be and incubated at 35 37 C for 18 48 hours. Additional periods of incubation may be necessary to recover some anaerobes. Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, pipets, saline blanks, slides, staining supplies, microscope, incinerator/autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations
BHI will not provide complete information for the identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control
If used properly, this medium should support good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens. If used properly, this medium should support good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.

Organism Tested

ATCC #

Results

Time (Hours) 24 h 24 48 h 24 h 24 48 h 24 48 h 24 h 24 48 h 24 h 24 h 24 h

Bacteroides fragilis Prevotella melaninogenica Bacteroides vulgates Fusobacterium nucleatum Fusobacterium necrophorum Clostridium perfringens Clostridium novyi Peptostreptococcus anaerobius Clostridium difficile Staphylococcus aureus

25285 25845 8482 25586 25286 13124 7659 27337 9689 25923

Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 96 hours. If the nutritive capacity of this media is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth.

Organism B. fragilis P. melaninogenica C. perfringens F. necrophorum S. aureus

Expected Growth 24 hrs 48 hrs 24 hrs 48 hrs 24 hrs

Physical Appearance: BHI should appear as a clear, yellowish liquid within the 16mm x 100mm glass tube.