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Lab 1 Photosynthesis

The lab activity aims to investigate the effect of light wavelength on the rate of photosynthesis in Elodea by measuring oxygen production. Students will use colored cellophane to filter light and collect data on bubble production as an indicator of photosynthesis. Safety precautions and a structured procedure are provided to ensure a successful experiment.

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Diana Aridi
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0% found this document useful (0 votes)
6 views3 pages

Lab 1 Photosynthesis

The lab activity aims to investigate the effect of light wavelength on the rate of photosynthesis in Elodea by measuring oxygen production. Students will use colored cellophane to filter light and collect data on bubble production as an indicator of photosynthesis. Safety precautions and a structured procedure are provided to ensure a successful experiment.

Uploaded by

Diana Aridi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Ajial Bilingual School Lesson 9.

2: Photosynthesis

Student’s name: ……………………… Gr 9 ………

Lab Activity: Effect of Light Wavelength on Photosynthesis in Elodea

Objective: To investigate how distinct colors (wavelengths) of light affect the rate of photosynthesis in
Elodea, using the amount of oxygen gas produced as an indicator.

Materials (per group):

 1 x 500 mL beaker

 1 g sodium bicarbonate (NaHCO₃)

 1 funnel (clear plastic)

 1 test tube

 2–3 leaves of Elodea

 Distilled or spring water

 Rubber bands (3)

 Stirring rod

 Colored cellophane (blue, and green)

 Scissors

 Tape

 Grow light or sunny window

Safety Reminders:

 Wear goggles and gloves.

 Handle scissors and glassware carefully.

 Do not eat or drink in the lab.

 Wash hands thoroughly after the lab.

Prior Preparation (Teacher):

 Prepare colored cellophane wraps.

 Set up the light stations each with both: blue, and green.

 Ensure Elodea is healthy and submerged.


Procedure:

Prepare two samples of Elodea by following the instructions:

1. Fill the beaker 3/4 full of distilled or spring water.

2. Add 1 g sodium bicarbonate and stir to dissolve.

3. Place 2–3 Elodea leaves into the narrow end of the funnel (cut to fit if needed).

4. Invert the funnel and submerge it in the beaker.

5. Fill a test tube completely with water. Invert it while submerged and place it over the funnel stem
without trapping air.

6. Secure the test tube in place using rubber bands.

7. Wrap the blue and green cellophane around each beaker. Tape securely.

8. Place the 2 beakers under a white light source.

9. Begin timing.

10. Count and record the number of bubbles produced every 5 minutes.

Data Collection (20 minutes)

Time (min) Number of Bubbles

10

15

20

Cleanup & Sharing (5 minutes)

11. Disassemble your setup. Clean and return materials.

12. Share your data with the class for group comparison.

Guiding Questions for the research part

1. Why does chlorophyll reflect green light while absorbing other wavelengths like blue?

2. How does the absorption of blue light compare to green light in terms of energy efficiency for
photosynthesis?

3. Which filter color (blue or green) resulted in a higher rate of photosynthesis in your experiment?
Why?

4. How do your results help explain why plants appear green?

5. How might this experiment be applied to improve artificial lighting for indoor plant growth?
Conclusion: Write 2–3 sentences summarizing what you learned from this lab:

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