Writing up Science Based Practical Reports

Eight Top Tips for Writing up Science Based Practical Reports

1. General Format of Laboratory Reports

The most common format for a scientific paper is to have sections headed as follows:
- Introduction (sets the scene for the work; include suitable references; states clearly the
aims/objectives; explains why you are doing the work. May require an abstract, summary of
findings or aims of the experiment as required by the brief)
- Materials and Methods (rarely reported separately but incorporated in the description of the
method; write about what you did in order i.e. “the sample was mounted on a microscope slide and
a labelled diagram prepared” not “labelled diagrams were prepared after the sample was mounted
on a microscope”; give enough detail for the work to be repeated by any reader)
- Results (presented in tables, figures or graphs; must be described in the text but the amount of
text should be kept to a minimum)
- Discussion (should include statements on what your results have shown but must not be a repeat
of the results section; discuss the significance of your observations; point out any limitations to
your methods, for example damaged samples; link your observations with references e.g. Figure 1
clearly shows leaf-like spicules of T.vitrinus a characteristic of Trichostrongylus species)
- Conclusion (final statement should be made about the validity of the results and the methods
used)
- References (all references used in the text should be listed at the end of the report)

1. Style of writing

Style should be simple and straightforward, avoiding elaborate vocabulary and long sentences.
Shorter sentences make it easier to understand what you have done and important points are
given more emphasis.

Write the ‘Materials and Methodology’ section in past tense – you have undertaken the work
and you are reporting what is in the past.

You should use an impersonal style when writing up your work. An impersonal style uses:
 the third person ( it rather than I or we)
 things rather than people as subjects of sentences.

Avoid: Instead use:

I observed the angle to be… The angle was observed to be…
I suggest… It is suggested… or The author suggests that…
We used a standard graphical representation A standard graphical representation was used
to… to…
I found… It was found that…
I assumed that… It was assumed that…
I noticed… Analysis of the raw data indicated…
In this report I show… This report presents…
2. Write exactly what you mean
Take care when using the words “proves” or “definitively”. Avoid the use of the term “significantly”
unless statistical analysis is taking place. Scientific claims are often expressed with cautious or
tentative language i.e.

Increased nitrate levels in bodies of water may cause eutrophication

instead of
Increased nitrate levels in bodies of water cause eutrophication.

Common words that can you can use:

Nouns Adverbs Verbs
Supposition Presumably Appear
Idea Probably Postulate
Speculation Possibly Suggest
Conjecture Apparently Seem
Possibility Not unlikely May be
Inference Seemingly Speculate

3. Write objectively
You must avoid bias, emotions or subjective writing (found in personal essays, in
autobiographies, and in the editorial section of newspapers where journalists express their
opinions about news events). Avoid using colloquialisms such as ‘massive change in …’ or ‘results
dramatically showed…’

Objective writing presents facts and figures only. It does not include the writer’s beliefs or
feelings.

Look at the examples below:

Avoid writing like this (subjective style)

These results seem to be really quite good. The model fits very well with the data points as can be
interpreted by the R2 values of 0.32 shown in Table 1 above. But the method used to obtain the best values
for a, b, and c was a little silly and time-consuming as it required putting lots of values into a changeable
Excel spreadsheet over and over to try and get the lowest R2 value, even though this is probably the only
way to do it accurately. Also, this model can be used to extrapolate the PCB concentrations of fish of ages
not measured in the study, but that's about it.

Instead write more objectively like the example below

These results appear to be reasonable as the model fits very well with the data points, as can be
interpreted by the R2 values of 0.32 shown in Table 1 above. However, the method used to obtain the best
values for a, b, and c was rather time-consuming as it required putting many values into an Excel
spreadsheet many times to obtain the lowest R2 value. While this is probably the only way to obtain
accurate results, a further limitation is that this model can be used only to extrapolate the PCB
concentrations of fish within age ranges measured in the study.
(Source: adapted from Skills@Library, 2010)
4. Use figures, tables and photographs to illustrate your points and results
What is a figure?
Graph, diagram, flowchart, photograph (some tutors may ask you to label photographs as ‘Plates’)
and maps etc.

What is a table?
Data that is presented in a labelled grid of columns and rows.

The famous saying ‘a picture is worth a thousand words’ illustrates that a figure, table or
photograph can really add to your work. If you insert one in your report then make sure you:

a) number it correctly i.e. the first figure in your report should be Figure 1, the
next Figure 2 etc…. the same goes for Tables

b) give it a title (insert these before for a Table and after for a Figure. Do not
write Figure 1. Graph to show…. Or Table 1: Table to show….)

Table 1. Bacteria counts on agar plate

Dilutions Absorbance Number

(X) of
bacteria
(Y)
Original E.coli
1/2
1/4
1/8
1/16

Figure 1. Using turbidimetry to estimate

bacterial numbers

c) refer to it i.e you must always refer to the table or figure in the text
before you come to it. Do not repeat details but summarise what
it shows e.g. “Grain yield response to successive increments of
applied nitrogen shows the law of diminishing returns (Figure 1
Table X)”.

d) source it you must always give the source of the data depicted -
Author’s surname/organisation name and year
– see Guide to Referencing 2012 (The Learning Hub/Support for
Learning/Study Advice)
5. Write clearly and concisely
George Orwell wrote some helpful rules for scientists who write. In summary he wrote:
a) “Never use a long word where a short one will do.
b) If it is possible to cut a word out, always cut it out” (Orwell, 1999).

Some examples of word savings are:

Superfluous words Better English
on account of the fact that as
in order to to
at the present time now
on the occasion of when
with the result that so that
in the college environment in college
it is apparent therefore thus
forward planning planning

6. Abbreviations
Abbreviations are commonly used in scientific writing in order to make your writing flow and to
avoid long technical words maximising your word count. Remember to always give the term in full
when it appears first in your report and show the abbreviations in brackets after i.e. “Magnetic
resonance imaging (MRI)” or “polymerase chain reaction (PCR)”.

7. Further Support
1. An excellent interactive resource developed by the University of Reading to help
undergraduate science students with their scientific writing.
Centre for Excellence in Teaching and Learning in Applied Undergraduate Research Skills. 2007. The
interactive resource for bioscience students. [On-line]. University of Reading. Available from:
http://www.engageinresearch.ac.uk/ [Accessed on 6th January 2012].

2. A US based interactive guide to writing lab reports that supports you through pre, during and
post laboratory activities.
Carter, M., Wiebe, E.N., Ferzli, M. and Wallace, R. 2004. Labwrite for students. [On-line]. NC State
University. Available from: http://labwrite.ncsu.edu/index_labwrite.htm [Accessed on 6th January 2012].

References
Orwell, G. 1999. Politics and the English language. [On-line]. www.orwell.ru. Available from:
http://www.orwell.ru/library/essays/politics/english/e_polit [Accessed on 6th January 2012].

Skills@Library. 2010. Writing for Science Subjects: 10 Characteristics of Scientific Writing. [On-line]. University of
Leeds. Available from: http://skills.library.leeds.ac.uk/uploads/Writing%20for%20Science%20Subjects747.pdf
[Accessed on 6th January 2012].
EXAMPLE - DETAILED LAB REPORT
Does the choice of winter or spring cropping influence the response of soil fauna to
cultivation?

Studies have shown that the loss of over-winter stubble, as a result of a shift from spring to winter
cultivation, results in a negative impact upon the availability of food for farmland birds over winter
and through the breeding season. This investigation seeks to determine whether soil invertebrates
are significantly affected by the choice of cultivation season, in particular with reference to those
families important for farmland birds. The results revealed that cultivation season did not have a
significant effect on the number and composition of soil invertebrates, nor did the effect of
cultivation. These results, however, may not be truly indicative and discrepancies in experimental
sampling techniques may have flawed the experiment.

Keywords: soil cultivation, cultivation season, soil invertebrates, soil fauna, farmland birds

Introduction

Soil cultivation has varied in style and purpose throughout UK arable agriculture’s history.
Traditionally soil inversion was undertaken as a measure to remove previous stubble and weeds,
re-establish organic matter into the soil and prepare a favourable seed bed for the new crop. After
the Second World War, the majority of cultivation shifted from spring to winter in response to the
appearance of winter-hardy, high yielding varieties onto the market (Carter & Stansfield, 1994).
This led to the wide scale loss of over-winter stubbles on arable land (Robinson and Sutherland,
2002). Studies have shown that this loss of stubble impacts upon the availability of winter food, in
the form of weed seeds, for farmland birds and adversely affects the provision of a fallow sward
rich in seeds and invertebrates for the breeding season (Vickery et al., 2004).

Knowledge of the dynamics and diversity of soil biota is particularly limited in comparison to above
ground organisms, particularly at the species level (Bardgett, 2005). Animal biomass is dependent
on soil conditions, but may often exceed 0.5t/ha. Where earthworms (Lumbricidae) are abundant,
they represent the bulk of this biomass (Killham, 1994). Soil invertebrates may exist entirely within
the soil (Endogeics), in burrows emerging to feed on surface litter (Anecics) or entirely in the litter
layer (Epigeics) (Lavelle and Spain, 2001.) Soil fauna benefit from an extensive diversity of prey,
feeding habits, mobility and tolerance to disturbance. Bardgett (2005) states that recent interest in
soil communities has been driven by the awareness that soil organisms regulate major ecosystem
processes such as organic matter turnover and nutrient cycling and act as drivers for change in
vegetation. The activity of soil biota is therefore significant in agriculture particularly as some
fauna, mainly earthworms, are seen as indicators for soil quality and others, such as ground
surface beetles, are useful for pest control (Colman and Crossley, 2003). Other soil invertebrates
are pest species themselves, for example the beetle larvae of the wire worm (Elateridae) family.

In concurrence with the current focus of relevant UK authorities on farming for conservation, most
contemporary studies into soil fauna focus on the effects of reduced tillage on populations in
contrast to conventional tillage effects. The majority of studies have found invertebrate abundance
is significantly lower in conventionally tilled soils (Anderson, 2003; Brennan et al., 2006; Colman
and Crossley, 2003; Holland, 2004; Wall, 2004). The less disturbance a soil has been subjected
to, the greater the abundance and diversity of soil animals (Killham, 1994). Cultivation may
therefore be regarded as a form of habitat destruction causing a significant alteration to the soil
ecosystem.

As a result of the aforementioned changes in arable season, conservation concerns have arisen
about the effects of the loss of over-winter stubble to agro-ecosystem diversity, in particular with
respect to farmland birds. In this respect, a study by Holland et al. (2006) gives the most important
orders of invertebrate to be adult beetles (Coleoptera), adult bugs (Hemiptera), butterflies and
moths (Lepidoptera), adult and larval flies (Diptera) and spiders (Arachnida). Particular families of
importance include Carabidae, Curculonidae, Elateridae and Lumbricidae.

This investigation seeks to determine in addition whether soil invertebrates are significantly
affected by the choice of cultivation season. This will be studied in terms of diversity/composition
and total number. In accordance with the importance of farmland birds as a UK quality of life
indicator, this study will pay particular attention to a selection of families deemed most important
for 4 or more UK farmland bird species.

Hypotheses to be tested:
H0: Time of ploughing does not affect invertebrate community composition
H1: Time of ploughing does affect invertebrate community composition

H0: Abundances of families Carabidae, Curculonidae, Elateridae and Lumbricidae are not affected
by time of ploughing
H1: Abundances of families Carabidae, Curculonidae, Elateridae and Lumbricidae are affected by
time of ploughing

H0: Time of ploughing does not affect total number of soil invertebrates present
H1: Time of ploughing does affect total number of soil invertebrates present

Materials and Methods

This study was carried out at the University of Reading Sonning Farm, Berkshire. The site has
been previously used for a range of agricultural trials. The area chosen (outlined in red on Figure
1) has been under production periodically in recent years. During the winter season 2005/2006 the
area had undergone three separate trials involving intense cultivation and production on the soil.
Following this, the site was left in stubble for the winter season 2006/2007.

A fully randomised block experimental design was used for this study. Nine plots were arranged at
random within an area approximately 10 m². The orientation of the plots was also randomised.
Each plot was split into three 0.5 m² blocks. These three blocks were designated control, autumn
plough and spring plough at random. Initial condition soil samples were taken at this stage
(07/11/06). The estimated sample dimensions were 15x15x10cm. Throughout this study, samples
were dug using a spade and placed into polythene bags. Following the taking of pre-treatment
samples, the blocks designated for the autumn treatment underwent simulated ploughing by
turning over the soil with a spade to an approximate depth of 15cm. Care was taken to ensure that
the soil of the adjacent blocks was not disturbed. The initial conditions samples were sorted in the
laboratory over a period of 20 minutes for each sample and animals discovered were placed into
bottles and preserved in alcohol.
Figure 1. Previous land use at the Sonning Farm field site 05/06 and 06/07

On 20/02/07 simulated ploughing was conducted on the spring plots. Again the soil was turned
over to an approximate depth of 15cm and care was taken not to disturb the soil of the adjacent
plots. On 27/02/07 the treatment samples were taken from the centre of each of the plots. The
sample dimensions were consistent with the initial conditions samples. Samples were once more
transported to the lab in polythene bags, hand-sorted and the specimens removed were preserved
in alcohol.

Following this, keys were used to identify the specimens to order level. Particular attention was
paid to coleoptera larvae which were identified to family level.
The results were analysed using one way ANOVA tables and a P value of 0.05 for confidence. In
addition t-testing was used to show any relevant differences between pre-treatment and treatment
means.

Results

A distinct lack of significant difference between the autumn tilled, the spring tilled and the control
plots was found in the pre-treatment initial condition samples. The average number of
invertebrates pre-treatment shows a weak but non-significant (P=0.244) decrease in number from
control to autumn to spring (Figure 2). The diversity of invertebrates pre-treatment follows the
same weak non-significant (P=0.472) pattern (Figure 3).

Figure 2. Average number of invertebrates from plots before treatment

Figure 3. Average diversity of invertebrates from plots before treatment

The treatment does not appear to have a significant effect on the number of invertebrates present
in plots. Although the number of invertebrates decreased in the spring treatment compared to
autumn tilled and the control, this was not significant (P=0.200; Figure 4). The diversity of
invertebrates was highest in the control but showed no significant (P=0.578) pattern (Figure 5).
Curiously, the total number of invertebrates found rose after treatment (t=-1.71, P=0.092).

Figure 4. Average number of invertebrates from plots after treatment

Figure 5. Average diversity of invertebrates from plots after treatment

In general, the changes in individual families important for farmland birds decreased slightly from
control to autumn to spring in both the pre-treatment and the treatment samples although the
change was not significant (Tables 1 & 2). There appears to be an increase in number from pre-
treatment to treatment especially in the case of earthworms (Control t=1.44, P=0.172. Autumn
t=1.39, P=0.184. Spring t=2.30, P=0.036).

Discussion

The results revealed that cultivation season did not have a significant effect on the number and
composition of soil invertebrates. Unusually, they also implied that cultivation itself had no or very
little effect which is contrary to previous research
(Killham, 1994; Anderson, 2003; Colman and Crossley, 2003; Wall, 2004; Holland,
2004). The grounds for the assertion that cultivation must affect soil invertebrates are plentiful and
convincing; at its most basic, soil disturbance itself may kill or displace organisms. Smeaton et al.
(2003) revealed that habitat disruption and physical damage are the principal causes of earthworm
population reduction after traditional cultivation, an effect that can be reduced by the use of a min-
till system (Schmidt and Curry, 2001; Birkas et al., 2004). The loss of weed cover and surface litter
can adversely affect epigeic species, in particular adult Carabid beetles (Kromp, 1999). Cantharis
beetles are also adversely affected by surface vegetation loss as 75% of their life cycle is spent in
larval stages reliant on surface vegetation cover (Traugott, 2006). Soil disturbance may also
unfavourably affect the abundance and quantity of fungi present in the soil, therefore reducing the
populations of fungivorous invertebrates such as Collembola (Brennan et al.,2006). The changing
fungi prevalence leads to a seasonal dependence of fungi-reliant species, a dependence which is
not evident in communities based under minimum-tillage arable systems (Beare, 2004).

However there are also some arguments that seasonal soil disturbance may have a minimal or
even encouraging effect on particular invertebrate families. Purvis and Fadl (2002) found that
cultivation season has a limiting effect on the flexibility of Carabid life-cycles although they show
robustness in their response to the constraints. It has additionally been shown that some beetle
species, including T. quadristriatus, are more abundant in autumn cultivated soils and are
dominant in conventional agricultural fields as a result of life-cycle adaptation (Anderson, 2003).

Aside from natural seasonal variation in the numbers of particular orders, further more universally
impacting environmental factors may have had an effect on the results of this investigation. The
initial conditions samples were taken on a dry day in November whilst the treatment samples were
taken in February after a period of rain. This might be responsible for the greater number of
invertebrates found in the treatment samples than in the initial samples. Another important
observation for the purposes of these results is the wet soils made the manner of sorting of the
treatment samples different from the dry soils sorted in the pre-treatment samples. This too could
have had an effect on the number of invertebrates found whilst sorting.

For practicality, the plot sizes chosen in this experiment were small at 0.5m² which did not allow
for soil disturbance by farm equipment. Ploughing was simulated using spades to a depth of
approximately 15cm, a depth recommended to avoid burying surface nutrients below the extent of
crop roots. However it is widely agreed that farm equipment has effects on the soil other than
simple disturbance, for example compaction, and it is dubious whether disturbance with a spade
would accurately mimic ploughing with farm equipment.

In addition to using the spade for soil disturbance, sampling with a spade selects for less mobile
invertebrate species. More larvae than adult beetle families were found probably due to the high
mobility of the adults allowing them to move away from the sampling area. The lack of mobility on
the part of the beetle larvae is said to be useful in reflecting habitat quality (Juen and Traugott,
2004). Issues of mobility may also explain why few arachnids were sampled despite the family
being described as the most abundant epigeic predator (Lavelle and Spain, 2001).

Timing issues may also have had an effect on the composition and number of invertebrates found
in each plot. The soil of the autumn tilled plots had lain undisturbed for over three months whereas
the spring tilled plots had only one week in a disturbed state before sampling was undertaken.
This does not seem to have had an effect on the results but may have counted against the
experiment should any significant patterns have occurred. Therefore in any repetition of this work,
further consideration of ploughing sampling times is advised. Another fault with the chosen
sampling method was an inconsistency in the mass of soil samples taken by different people and
with different sizes of spade. Although every effort was taken to approximate the given dimensions
as accurately as possible, this task was managed dissimilarly by different people. Use of an auger
would produce more standardised sample sizes however this investigation was limited in
resources and time.

Further inconsistencies were introduced in the sorting phase as different people were likely to
have varied proficiencies and methods of sorting through soil. Furthermore various proficiencies in
identification may have also affected the results. Further collaboration between involved parties, or
single-person efforts in sampling, sorting and identification may reduce these errors although there
may still be issues with waning enthusiasm or increased proficiency throughout each stage.
Increased proficiency in addition to the moisture of the soil may go some way towards explaining
the higher amount of invertebrates found in the treatment samples.

In conclusion it was not possible to reject the following null hypotheses given the results of this
investigation:
H0: Time of ploughing does not affect invertebrate community composition
H0: Abundances of families Carabidae, Curculonidae, Elateridae and Lumbricidae are not affected
by time of ploughing
H0: Time of ploughing does not affect total number of soil invertebrates present

However in light of scientific literature to the contrary it may be worthwhile repeating this
experiment with the improvements listed above, namely standardising soil sample sizes, using
true ploughing equipment, improved timing of sampling and increased effort to reduce bias
between participating individuals. Following this, further study might include a greater focus on the
farmland bird important families and along with study over a larger area, correlation between
invertebrate abundance and farmland bird abundance.

With the current focus on farming conservation and agricultural methods constantly shifting to
improve the relationship between farming and environment; more extensive monitoring of the
effects on invertebrates and the wider food chain are becoming increasingly important as a means
of verification that conservation farming is having a long-term and significant positive effect.
References

Andersen, A. 2003. Long-term experiments with reduced tillage in spring cereals. Effects on pests
and beneficial insects. Crop Protection, 22, pp. 147-152.

Bardgett, R. 2005. The biology of soil, a community and ecosystem approach.

Oxford: Oxford University Press.

Beare, M.H. 2004. Organic matter decomposition and nitrogen mineralization. In:
D.H. Wall. ed. Sustaining Biodiversity and Ecosystem Services in Soils and Sediments. USA:
Island Press.

Birkas, M., Jolankai, M., Gyuricza, C. and Percze, A. 2004. Tillage effects on
compaction, earthworms and other soil quality indicators in Hungary. Soil and Tillage
Research, 78, pp. 185-196.

Brennan, A., Fortune, T., Bolger, T. 2006. Collembola abundances and assemblage
structures in conventionally tilled and conservation tillage arable systems. Pedobiologia, 50,
pp.135-145.

Carter, E.S. and Stansfield, J.M. 1994. British Farming, Changing Policies and
Production Systems. Ipswich: Farming Press Ltd.

Colman, D.C. and Crossley, D.A. 2003. Fundamentals of Soil Ecology. USA: Elsevier Science.

Holland, J.M. 2004. The environmental consequences of adopting conservation tillage in Europe:
reviewing the evidence. Agriculture, Ecosystems and Environment, 103, pp. 1-25.

Holland, J.M., Hutchinson, M.A.S., Smith, B. and Aebischer, N.J. 2006. A review of
invertebrates and seed bearing plants as food for farmland birds in Europe. Annals of Applied
Biology, 148, pp. 49-71.

Juen, A. and Traugott, M. 2004. Spatial distribution of epigaeic predators in a small field in relation
to season and surrounding crops. Agriculture, Ecosystems and Environment, 103, pp. 613-620.

Kromp, B. 1999. Carabid beetles in sustainable agriculture: a review on pest control

efficacy, cultivation impacts and their enhancement. Agriculture, Ecosystems and
Environment, 74, pp. 187-228.

Lavelle, P. and Spain, A.V. 2001. Soil Ecology. Netherlands: Kluver Academic Publishers.

Purvis, G. and Fadl, A. 2002. The influence of cropping rotations and soil cultivation
practice on the population ecology of carabids (Coleoptera:carabidae) in arable land.
Pedobiologia, 46, pp. 452-474.

Robinson, R.A. and Sutherland, W.J. 2002. Post-war changes in arable farming and
biodiversity in Great Britain. Journal of Applied Ecology, 39, pp. 157-176.

Schmidt, O. and Curry, J.P. 2001. Population dynamics of earthworms (Lumbricidae) and their role
in nitrogen turnover in wheat and wheat-clover cropping systems. Pedobiologia, 45, pp. 174-187.

Smeaton, T.C., Daly, A.N. and Cranwell, J.M. 2003. Earthworm responses to cultivation and
irrigation in a South Australian soil. Pedobiologia, 47, pp. 379-385.

Traugott, M. 2006. Habitat use and activity patterns of larval and adult Cantharis
beetles in arable land. European Journal of Soil Biology, 42, pp. 82-88.