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Blood Typing PreLab Discussion

The document outlines the process and objective of blood typing, which includes determining an individual's blood type and understanding the blood typing process using known antisera. It details the materials needed, safety precautions, and step-by-step instructions for obtaining a blood sample and performing the test. The results are interpreted based on agglutination patterns to identify blood types A, B, AB, O, and Rh factor.

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enoch taclan
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22 views13 pages

Blood Typing PreLab Discussion

The document outlines the process and objective of blood typing, which includes determining an individual's blood type and understanding the blood typing process using known antisera. It details the materials needed, safety precautions, and step-by-step instructions for obtaining a blood sample and performing the test. The results are interpreted based on agglutination patterns to identify blood types A, B, AB, O, and Rh factor.

Uploaded by

enoch taclan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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BLOOD TYPING

Enoch Caryl Taclan BSBiol MScBiol*


Objective

● To determine one’s blood type


● To appreciate the process of blood typing.
● To determine ABO Blood Group by testing the
sample red blood cells with known antisera
Discussion • Blood grouping may be performed
on the slide for the red blood cells.
● Determination of blood
groups is made with • Drying of the specimen is faster if
Anti-A, Anti- B and Anti- slide method is used and may be
AB, blood tying sera to mistaken for false positive reaction.
demonstrate the
• In addition, hemolysis is not
presence of the antigen
detected in slide method.
on the red cells.
• If the methods described below
differ from the recommended
procedures given by the
manufacturer of the antiserum in
use, the manufacturer's instructions
should be used.
Materials for Blood typing
• Glass Slides
• Disposable droppers
• Toothpicks
• Anti-A typing sera
• Anti-B typing sera
• Alcohol Swab
• Blood sample
• Lancet
• Gloves
• Cotton balls
Before the Procedure
1. Follow safety 2. Prepare the 3. Prepare the
precaution following: following:
▪ Wear gloves while
• clean glass slide, • Divide the glass
performing blood
two new slide in 3 portion
tests •
and mark anti-A,
toothpicks, anti-B and RH
▪ Place blood slides • pencil,
and toothpicks in a • lancet,
biohazard
• cotton balls,
container after the
activity
• 70% alcohol
• anti-A and anti-B
▪ Clean your area at sera
the end of the
laboratory activity
Obtaining Blood Sample
❑ Wash your hands thoroughly with soap and water. Dry
with clean paper towel

❑ Wipe the fingertip of your ring finger with a cotton ball


soaked in 70% alcohol. Allow alcohol to dry without
wiping.

❑ Open a new, sterile blood lancet exposing the sharp


tip only.

❑ Puncture the cleaned fingertip with a sterile lancet.


Never reuse a lancet, even your own.

❑ Deposit the used lancet in the sharp's container for


biohazard materials only.
Obtaining Blood Sample
❑ Wipe away the first drop of blood with a cotton ball and
dispose in a biohazard wastes container.

❑ Gently squeeze one drop of blood on each side of a clean


prepared slide without the finger touching the slide.

❑ Add a drop of Anti-A serum (group B) to the blood drop on


side A. Add a drop of anti-B serum (group A) to the blood
drop on slide B.

❑ Mix the serum and blood cells using separate and clean
toothpicks. Observe closely what happens to the mixture.

❑ Determine presence/absence of agglutination or clumping


of cells.
Obtaining Blood Sample
❑ Determine the student's blood type based on the
reaction pattern:
✓ Agglutination with anti-A reagent = Type A

✓ Agglutination with anti-B reagent = Type B

✓ Agglutination with both = Type AB

✓ No agglutination with both = Type O

✓ Agglutination with anti-Rh reagent = Rh-positive

(Rh+)
✓ No agglutination with anti-Rh reagent = Rh-negative

(Rh-)
Blood Typing Chart
Answer the post lab questions in your
activity
Thanks!
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