Bacteriology Lecture 2
Bacteriology Lecture 2
CLINICAL BACTERIOLOGY
Professor: Ethel Mangada
MICROBIAL TAXONOMY
o D. Class: contains similar
orders
Taxonomy
o E. Order: contains similar
Area of biologic science comprising families
three distinct but highly interrelated o F. Family: contains similar
disciplines: genera
o Classification Group of organisms that
e.g, eukaryote, may contain multiple
prokaryote genera and consists of
o Nomenclature (naming) organisms with a
o Identification of organisms common attribute
e.g., genotypic and Example:
phenotypic identification Enterobacteriaceae,
Orderly classification and grouping of Streptocaccaceae
organisms into taxa (categories) o G. Genus: contains similar
Bases on similarities and differences in species
genotype and phenotype Based on various genetic
and phenotypic
Carl von Linne / Carolus Linnaeus
characteristics shared
Laid down the basic rules for among the species
taxonomic categories o H. Species: specific epithet
Most basic of the
taxonomic groups and
Classification can be defined as a
collection of bacterial
Method for organizing microorganisms
strains that share
into groups or taxa based on similar
common physiologic and
morphologic, physiologic, and genetic
genetic features and
traits
differ notably from other
Hierarchical classification system
microbial species
consists of the following taxa
Subspecies:
designations:
taxonomic
o A. Domain: Bacteria,
subgroups within
Archaebacteria, Eukarya
species
o B. Kingdom: contains similar
o Species
divisions or phyla
subdivided
based on
o C. Phylum: contains similar
phenotypic
classes; equivalent to the
with the
Division taxa
CLINICAL BACTERIOLOGY
Professor: Ethel Mangada
MICROBIAL TAXONOMY
following o The family
differences o A genus
o A. Biotype- o A specie
considered Species definitions are distinguished
the same using DNA Profiling, including a
species nearly complete 16S rRNA sequence
with the in combination with phenotypic traits
same
characterist
ic genetic Nomenclature
makeup
that Naming of microorganisms according
displays to established rules and guidelines set
differential forth in the International Code of
physiologic Nomenclature of Bacteria (ICNB) or
characterist Bacteriological Code (BC)
ics Genus designation- first letter is
o B. always capitalized
Species designation- first letter is
Serotype-
lowercase
based on
serologic **Two components are used simultaneously
differences and are printed in Italics or underlined in
script
Example: Streptococcus pneumoniae,
Streptococcus pyogenes, Streptococcus
agalactiae, and Streptococcus bovis
Note: Dear King Philip Came Over For Good When bacteria are referred to as a
Soup group, their names are neither
capitalized nor underlines (e.g.,
Example :
staphycocci)
Family: Micrococcaceae
Genus: Staphylocci
Specie: Staphyloccocus aureus
Identification
Note!
Process by which microorganism’s key
Diagnostic microbiologist traditionally features are delineated
emphasize placement and naming of
bacterial species intro three
(occasionally four or five) categories:
CLINICAL BACTERIOLOGY
Professor: Ethel Mangada
MICROBIAL TAXONOMY
Process of discovering and recording
the traits of organisms so that they
may be placed in overall taxonomic
scheme
Organism can be then assigned to the
most appropriate taxa (classification)
and can be given appropriate genus
and species names (nomenclaturw)
Identification Methods
Genotypic characteristics- relate to
an organism’s genetic makeup,
including the nature of organism’s
genes and constituent nucleic acids
Phenotypic characteristics
Based on features beyond the genetic
level and include both readily
observable characteristics and
characteristics that may require
CLINICAL BACTERIOLOGY
Professor: Ethel Mangada
MICROBIAL TAXONOMY
Examples: macroscopic (colony
morphology on media) (e.g., E. coli- Major Characteristics Used in
greenish with metallic sheen ) and Taxonomy
microscopic (size, shape, arrangement
into groups or chains of organisms) Classical Characteristics
morphology, staining characteristics o useful in routine identification of
(gram- positive or gram- negative), phylogenetic information
nutritional requirements, physiologic Ex: morphology,
and biochemical characteristics, and physiology and
metabolism, ecology and
genetic analysis
o Phylogenetic and phyletic
classification is based on
evolutionary relationships
instead of general resemblance
Molecular characteristics
o Based on the study of nucleic
acid composition and proteins
susceptibility or resistance to
antibiotics or chemicals