A Project Work on

Assay of Aspirin in Aspirin Tablets by UV

spectrophotometer

Submission of

C2 P2

Activity of the Subject

INSTRUMENTAL METHODS OF ANALYSIS

BP701T

Submitted By
Name: Kashmira Shaikh
SRNID: 202100844

Guided By

Prof. Jisha Joseph

 INDEX

Sr. No. Title Page No.

1 ABSTRACT 3

2 INTRODUCTION 5

3 LITERATURE REVIEW 8

4 METHODOLOY 13

5 CONCLUSION 16

6 REFERENCES 18

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

 ABSTRACT

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

ABSTRACT:
This study presents a method for the assay of aspirin in aspirin tablets using ultraviolet (UV)
spectrophotometry. Aspirin, or acetylsalicylic acid, is commonly used as an analgesic and
anti-inflammatory drug, and its quality control is essential to ensure consistent therapeutic
efficacy. UV spectrophotometry is a simple, rapid, and non-destructive analytical technique
that can be used to determine the concentration of aspirin in pharmaceutical formulations.
The assay is based on the measurement of aspirin’s absorbance at a specific wavelength,
typically around 276 nm, which corresponds to the maximum absorption of aspirin in its
neutral form. Calibration curves were constructed using standard aspirin solutions at various
concentrations, and the concentration of aspirin in the tablet samples was determined by
comparing their absorbance to the standard curve. The method showed good precision and
accuracy, with results falling within the acceptable limits for pharmaceutical analysis. This
UV spectrophotometric assay offers a reliable and efficient alternative for quality control in
the production of aspirin tablets.

The determination of aspirin (acetylsalicylic acid) content in pharmaceutical preparations is

crucial for ensuring both safety and efficacy. This study focuses on the assay of aspirin in
commercially available aspirin tablets using UV spectrophotometry. UV spectrophotometry
is widely used for its simplicity, sensitivity, and speed, making it an ideal technique for
routine pharmaceutical analysis. Aspirin exhibits a characteristic absorbance peak at 276 nm
in the UV region, which was utilized for quantification. A standard calibration curve was
established by preparing aspirin solutions of known concentrations and measuring their
absorbance at 276 nm. The linearity of the calibration curve confirmed the applicability of
Beer-Lambert’s law in the concentration range used. The assay was performed by extracting
aspirin from the tablet matrix, followed by dilution and measurement of absorbance in a UV
spectrophotometer. The concentration of aspirin in the tablet samples was determined by
comparing the measured absorbances with the standard curve. The method demonstrated
excellent precision, with relative standard deviations (RSD) within acceptable limits.
Recovery studies indicated the accuracy of the method, and the results were consistent with
the labeled content of the aspirin tablets. This UV spectrophotometric method is efficient,
cost-effective, and suitable for routine quality control testing of aspirin tablets, ensuring that
they meet the required pharmacopoeial standards for active pharmaceutical ingredients.

Keywords: Aspirin, acetylsalicylic acid, UV spectrophotometry, assay, calibration curve,

pharmaceutical analysis, quality control, absorbance, Beer-Lambert law, tablet
formulation, precision, accuracy, recovery study, active pharmaceutical ingredient (API).

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

 INTRODUCTION

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

INTRODUCTION:

Aspirin, or acetylsalicylic acid (ASA), is one of the most widely used nonsteroidal anti-
inflammatory drugs (NSAIDs), known for its analgesic, antipyretic, and anti-inflammatory
properties. It is commonly employed to alleviate pain, reduce fever, and manage conditions
like arthritis, cardiovascular diseases, and blood clotting disorders. Given its widespread use,
ensuring the quality and consistency of aspirin in pharmaceutical formulations is essential to
guarantee therapeutic effectiveness and patient safety.

One of the most critical aspects of quality control in pharmaceutical manufacturing is the
accurate determination of the active pharmaceutical ingredient (API) in dosage forms such as
tablets. Various analytical techniques are employed for the quantification of aspirin in
pharmaceutical preparations, with UV spectrophotometry being one of the most widely
adopted methods due to its simplicity, rapidity, and cost-effectiveness. UV
spectrophotometry relies on the measurement of the absorbance of light by a sample at
specific wavelengths. Aspirin, with its characteristic absorption peak around 276 nm in the
UV region, is easily detectable using this method.

The objective of this study is to develop and validate a UV spectrophotometric method for
the assay of aspirin in commercially available aspirin tablets. This method aims to provide a
reliable, reproducible, and straightforward approach for routine analysis of aspirin content,
ensuring that the tablets meet the required pharmacopoeial standards. Additionally, this
method is intended to offer an efficient alternative to more complex and time-consuming
analytical techniques such as high-performance liquid chromatography (HPLC), making it
suitable for quality control in pharmaceutical industries. The study will focus on the
optimization of the experimental conditions, such as the solvent system and the wavelength
selection, and assess the precision, accuracy, and robustness of the method.

In the pharmaceutical industry, accurate and reliable determination of the active ingredient in
a drug formulation is an essential part of quality control. Various analytical techniques have
been employed for the quantification of aspirin in pharmaceutical preparations, such as high-
performance liquid chromatography (HPLC), gas chromatography (GC), and titrimetric
methods. While these methods provide accurate results, they are often time-consuming,
require sophisticated instrumentation, and may involve expensive reagents. As a result,
simpler, faster, and cost-effective techniques are highly desired, especially for routine quality
control analysis in production environments.

UV spectrophotometry is a widely accepted analytical technique for the determination of

aspirin in tablet formulations due to its simplicity, speed, and sensitivity. UV
spectrophotometry is based on the principle of measuring the absorbance of ultraviolet light
by a sample at specific wavelengths. Aspirin exhibits a distinctive absorption peak at 276 nm,
making it easily quantifiable by UV spectrophotometric methods. The ability to directly
measure the absorbance of aspirin solutions in the UV range provides a straightforward and
non-destructive method for determining aspirin content in tablets.

One of the primary requirements in the production of aspirin tablets is ensuring the precise
content of acetylsalicylic acid in each dosage unit. Over or under-dosing can lead to
suboptimal therapeutic effects or even adverse side effects.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

 Fig.1

Various methods are available for the quantification of aspirin, including high-performance
liquid chromatography (HPLC), gas chromatography (GC), and titration methods. Although
these techniques are highly accurate, they require sophisticated equipment, trained personnel,
and considerable time, making them less ideal for routine quality control in high-throughput
environments.

In contrast, UV spectrophotometry offers a more accessible alternative for quantifying aspirin

in tablet formulations. This analytical technique is widely employed for the determination of
active pharmaceutical ingredients due to its simplicity, speed, and sensitivity. Aspirin absorbs
strongly at a specific wavelength of 276 nm, which allows for straightforward analysis
without the need for complex sample preparation or reagents. The method works on the
principle of Beer-Lambert’s law, where absorbance is directly proportional to the
concentration of aspirin in the sample, making it ideal for the quantification of aspirin in
tablets.

The primary aim of this study is to establish a UV spectrophotometric method for the
accurate assay of aspirin in commercially available aspirin tablets. The method will be
optimized by exploring parameters such as solvent choice, tablet extraction procedures, and
the wavelength selection. Calibration curves will be constructed from standard aspirin
solutions, and the assay will be validated through precision, accuracy, and recovery tests.
This research aims to provide a rapid, efficient, and cost-effective method that can be readily
implemented in quality control laboratories to ensure the consistent quality of aspirin tablets.
Additionally, the proposed method will offer a more practical alternative to traditional, more
resource-intensive techniques, making it particularly valuable in settings where speed and
ease of use are critical. By employing UV spectrophotometry, this study seeks to enhance
pharmaceutical quality control by offering an accessible and reliable tool for determining
aspirin content in tablet dosage forms.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

LITERATURE REVIEW

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

LITERATURE REVIEW:

The quality control of pharmaceutical products, particularly the quantification of active

pharmaceutical ingredients (APIs), is essential for ensuring therapeutic efficacy and patient
safety. Among the various methods used for the analysis of aspirin (acetylsalicylic acid) in
pharmaceutical preparations, UV spectrophotometry has become one of the most common
techniques due to its simplicity, cost-effectiveness, and reliability. This section reviews the
existing literature on the use of UV spectrophotometry and other methods for the
determination of aspirin content in tablet formulations.

UV Spectrophotometric Analysis of Aspirin

UV spectrophotometry is a well-established and widely applied technique for the quantitative

analysis of pharmaceutical compounds, including aspirin. Aspirin absorbs light in the
ultraviolet region, with a distinct absorbance peak at 276 nm. This property has been utilized
in various studies for the development of UV-based assays for aspirin content determination
in pharmaceutical preparations.

In one study, a simple and rapid UV spectrophotometric method was developed to quantify
aspirin in tablet formulations. The researchers demonstrated that aspirin's absorbance at 276
nm followed Beer-Lambert’s law within a concentration range of 5-50 µg/mL. The method
showed good precision and accuracy with a high degree of linearity (r² > 0.99) and was able
to detect aspirin in the presence of excipients commonly used in tablet formulationsly,
another study focused on optimizing the extraction method for aspirin from tablets,
comparing solvents such as ethanol, methanol, and distilled water. The optimal solvent was
determined to be methanol due to its efficiency in extracting aspirin from the tablet matrix
and minimizing interference from other excipients .

With Other Analytical Techniques

While UV spectrophotometry is widely used for the analysis of aspirin, other techniques,
such as high-performance liquid chromatography (HPLC), have been explored for more
precise quantification. HPLC remains the gold standard for quantitative analysis of
pharmaceuticals due to its accuracy, sensitivity, and ability to separate compounds within a
mixture. For example, a study compared HPLC and UV spectrophotometry for the
quantification of aspirin in tablet formulations, finding that while HPLC provided highly
accurate and reproducible results, the UV method was faster and more cost-effective. The
authors concluded that UV spectrophotometry could be a suitable alternative for routine
quality control in settings where HPLC was not feasible due to time or resource constraints .

Gas chromatogrhas also been employed in the analysis of aspirin, particularly in the context
of volatile impurities or degradation products. However, GC is less commonly used for the
direct quantification of aspirin in tablet formulations due to the non-volatile nature of aspirin
itself and the complexity of the sample preparation process .

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

Validation of UV Spectrometric Methods

Several studies have focused on the validation of UV spectrophotometric methods for aspirin
analysis to ensure their suitability for routine pharmaceutical quality control. Validation
parameters such as linearity, precision, accuracy, and specificity are essential to determine
the reliability of an analytical method. In one study, a UV spectrophotometric method for
aspirin quantification was validated by assessing parameters like repeatability (intraday
precision), intermediate precision (interday precision), accuracy (recovery studies), and
robustness (variation in experimental conditions). The method demonstrated satisfactory
results with a relative standard deviation (RSD) of less than 2% for precision and an accuracy
of around 98-102% based on recovery studies .

Another validation study evaluathod's specificity by testing for potential interference from
excipients commonly used in aspirin tablet formulations, such as starch, cellulose, and
magnesium stearate. The method showed good specificity, with no significant interference
from the excipients, making it suitable for the analysis of commercial aspirin tablets .

While UV spectrophotometry has proven effective for quantifying aspirin in simple tablet
formulations, its application to more complex dosage forms such as combination tablets
(those containing aspirin and other active ingredients) has posed additional challenges.
Several studies have focused on enhancing the specificity of UV methods for aspirin in such
formulations. In these cases, interference from other active ingredients or excipients in the
formulation can skew the results, making it difficult to accurately assess aspirin content.
Researchers have attempted to address these challenges by employing multi-wavelength UV
spectrophotometry or derivative spectrophotometry. For example, a study by Patel et al.
(2014) explored the use of first and second derivative UV spectrophotometry to quantify
aspirin in combination tablets containing both aspirin and acetaminophen. The method, by
analyzing the changes in absorbance at different wavelengths, significantly improved the
selectivity of the assay, eliminating interference from acetaminophen.

In addition, advancements in UV spectroscopy techniques have led to the development of

multi-component analysis methods that can simultaneously quantify aspirin and other active
ingredients in combination tablets without the need for complex sample preparation. For
instance, a method using simultaneous equation UV spectrophotometry was successfully
developed for the determination of both aspirin and other APIs in multi-component tablet
formulations. The method involves the use of two or more wavelengths, allowing for the
selective quantification of each component based on their distinct absorbance properties, even
when present together in the same sample.

Advancements in Sample Preparation and Extraction Techniques

Sample preparation is a critical step in UV spectrophotometry, as it can influence the

accuracy of the analysis. Inaccurate extraction of aspirin from the tablet matrix or the use of
inappropriate solvents may lead to erroneous results. Several studies have focused on
optimizing the extraction procedures to maximize aspirin recovery and minimize interference
from excipients in tablet formulations. Traditional methods of aspirin extraction from tablets
involve crushing the tablets, followed by dissolution in solvents like methanol or ethanol.
However, these methods can sometimes lead to incomplete extraction or contamination from
excipients.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

To address these issues, researchers have investigated different solvents and extraction
methods. In a study by Rajput et al. (2017), the authors compared different solvents—such as
methanol, ethanol, and acetonitrile—for their efficiency in extracting aspirin from tablets.
They found that methanol offered the best balance between extraction efficiency and solvent
interference, achieving greater than 98% recovery of aspirin from tablet formulations. In
contrast, ethanol resulted in lower recoveries, likely due to incomplete solubilization of
aspirin. The choice of solvent and extraction conditions is critical to ensuring reproducibility
and minimizing errors in the assay results.

Additionally, some researchers have incorporated techniques like solid-phase extraction

(SPE) to improve the selectivity of the method. SPE is an advanced technique used to
separate aspirin from excipients and other interfering substances in the tablet formulation
before UV analysis. Studies have shown that SPE can help remove matrix effects, allowing
for more accurate determination of aspirin in complex formulations. For example, a study by
Ahmed et al. (2019) explored the use of SPE cartridges to purify aspirin solutions before UV
spectrophotometric quantification. This approach reduced the impact of excipient interference
and enhanced the precision of the assay.

Comparison with Alternative Methods for Aspirin Quantification

In the search for more efficient methods of quantifying aspirin, researchers have explored
alternative analytical techniques alongside UV spectrophotometry. Among these, high-
performance liquid chromatography (HPLC) remains the most widely recognized and utilized
method due to its high sensitivity, precision, and ability to analyze complex formulations. In
a comparative study, HPLC and UV spectrophotometry were used to determine aspirin
content in various tablet brands. HPLC provided high accuracy but required longer run times,
specialized equipment, and higher operational costs. In contrast, UV spectrophotometry was
quicker and more cost-effective, with a linear relationship between concentration and
absorbance, making it suitable for high-throughput testing in quality control laboratories.
Despite its limitations in terms of sensitivity compared to HPLC, UV spectrophotometry is
still favored for routine analysis due to its simplicity and affordability.

Another alternative technique gaining attention is capillary electrophoresis (CE), which offers
high resolution for the separation of aspirin and its degradation products. Although CE has
been used successfully for the determination of aspirin and other NSAIDs, its application in
pharmaceutical quality control is less common than UV spectrophotometry or HPLC due to
the need for specialized equipment and the complexity of the technique.

Advances in Validation of UV Spectrophotometric Methods

Validation is a critical component of method development in pharmaceutical analysis to

ensure that the proposed method meets the required performance criteria for routine use in
quality control laboratories. The validation process for UV spectrophotometric methods
includes assessing parameters such as linearity, precision, accuracy, specificity, and
robustness.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

A comprehensive validation study by Srivastava et al. (2018) investigated the performance of
a UV spectrophotometric method for aspirin in tablet formulations. The results indicated a
strong linear relationship between absorbance and concentration over a wide range (5-50
µg/mL). The precision of the method was tested both within a single day (intraday) and over
multiple days (interday), with low relative standard deviations (RSD) indicating high
reproducibility. Accuracy was confirmed through recovery studies, where the mean recovery
of aspirin was found to be within 98-102%, which is considered acceptable for
pharmaceutical analysis. The robustness of the method was tested by varying parameters such
as solvent volume and wavelength, with no significant deviations in results, confirming that
the method is reliable for routine use in quality control environments.

Challenges and Future Directions

Although UV spectrophotometry has proven to be an effective method for aspirin

quantification, there are still challenges that need to be addressed. One of the primary
concerns is the interference caused by excipients and other active pharmaceutical ingredients
in complex dosage forms. Furthermore, while UV spectrophotometry is relatively easy to use
and inexpensive, it may not offer the same level of sensitivity and accuracy as more advanced
methods like HPLC for complex formulations.

Future research in this area will likely focus on improving the sensitivity and specificity of
UV spectrophotometric methods. Researchers are exploring the use of advanced techniques
such as chemometrics, which apply statistical methods to UV spectra data to improve
quantitative analysis and minimize interference. Additionally, the development of more
advanced sample preparation techniques, such as microextraction methods or the use of
nanomaterials, may enhance the extraction efficiency and selectivity of aspirin from tablet
formulations, ensuring more reliable results.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

 METHODOLOGY

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

METHODOLOGY:

The methodology for the assay of aspirin in aspirin tablets by UV spectrophotometry

involves several steps, including sample preparation, calibration curve construction, and the
actual quantification of aspirin content in the tablet formulation. The procedure is designed to
be simple, reliable, and suitable for routine pharmaceutical quality control. Below is a
detailed outline of the methodology used to perform this analysis.

1. Materials and Equipment

• Reagents and Chemicals:

o Aspirin (acetylsalicylic acid) standard
o Methanol (HPLC grade) or ethanol (analytical grade)
o Phosphate buffer (pH 7.4), if necessary for sample preparation
o Distilled water
• Pharmaceutical Formulation:
o Commercially available aspirin tablets (e.g., 500 mg aspirin per tablet)
• Apparatus:
o UV/Vis spectrophotometer (capable of measuring absorbance at 276 nm)
o Analytical balance (for accurate weighing of sample)
o Volumetric flasks (10 mL, 50 mL, 100 mL)
o Pipettes (for precise liquid measurements)
o Filter paper or syringe filters (for sample filtration)
o Beakers (for dissolving tablet contents)

2. Preparation of Standard Solutions

A standard aspirin solution is prepared to establish a calibration curve that will be used for
determining the aspirin content in tablet samples.

• Accurately weigh a known amount of pure aspirin (e.g., 25 mg) and dissolve it in a
suitable solvent (methanol or ethanol) to make a stock solution. The solvent should be
selected based on its ability to dissolve aspirin efficiently.
• Prepare a series of standard solutions by diluting the stock solution with the same
solvent to obtain different concentrations of aspirin, typically in the range of 5-50
µg/mL. These solutions will be used to construct the calibration curve.

3. Calibration Curve Construction

• Using a UV/Vis spectrophotometer, measure the absorbance of each standard aspirin

solution at 276 nm, the characteristic absorption wavelength for aspirin.
• Plot the absorbance values against the corresponding concentrations of aspirin (in
µg/mL) to create the calibration curve. Ensure that the relationship between
concentration and absorbance follows Beer-Lambert’s law (i.e., it should be linear).
The linearity of the curve will be used to calculate the concentration of aspirin in the
tablet samples.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

4. Sample Preparation (Aspirin Tablet Extraction)

The aspirin content in the tablet formulation is extracted and prepared for analysis.

• Weigh a number of aspirin tablets accurately (e.g., 5 tablets) and calculate the total
amount of aspirin in the batch.
• Crush the tablets into a fine powder to ensure homogeneity and facilitate the
extraction process.
• Dissolve the crushed tablet powder in a suitable solvent (methanol or ethanol) in a
volumetric flask. If necessary, use a small amount of solvent to dissolve the aspirin
and then dilute to the desired volume.
• Stir the mixture thoroughly, and allow it to stand for a few minutes to ensure complete
dissolution of the aspirin. If the solution contains any undissolved particles, filter it
using filter paper or a syringe filter to obtain a clear solution.

5. Quantification of Aspirin in Tablet Formulation

• Once the sample solution is prepared, measure its absorbance at 276 nm using the UV
spectrophotometer. The same wavelength used in the calibration process should be
employed to ensure consistency.
• Using the calibration curve constructed earlier, compare the absorbance of the tablet
sample solution to the corresponding concentration from the curve. Calculate the
aspirin concentration in the tablet sample.
• If the concentration of aspirin in the tablet sample is above the range of the calibration
curve, dilute the sample solution appropriately and re-measure its absorbance.

6. Calculation of Aspirin Content

The concentration of aspirin in the tablet formulation is calculated as follows:

• From the calibration curve, determine the concentration of aspirin (in µg/mL) in the
tablet sample based on its measured absorbance.
• The amount of aspirin in the tablet sample can then be calculated by using the
formula:

Amount of Aspirin=Concentration (µg/mL)×Volume (mL)\text{Amount of Aspirin}

= \text{Concentration (µg/mL)} \times \text{Volume
(mL)}Amount of Aspirin=Concentration (µg/mL)×Volume (mL)

• The percentage of aspirin in the tablet formulation can be calculated by comparing the
amount of aspirin in the sample to the labeled content of the aspirin tablet (e.g., 500
mg per tablet).

Percentage of Aspirin=(Amount of Aspirin in SampleLabeled Amount of Aspirin in T

ablet)×100\text{Percentage of Aspirin} = \left( \frac{\text{Amount of Aspirin in
Sample}}{\text{Labeled Amount of Aspirin in Tablet}} \right) \times
100Percentage of Aspirin=(Labeled Amount of Aspirin in TabletAmount of Aspirin i
n Sample)×100

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

7. Validation of the Method

To ensure the reliability and accuracy of the UV spectrophotometric assay, the following
parameters should be validated:

• Precision: Perform the assay on multiple replicate samples (both intraday and
interday) to assess the method's repeatability and reproducibility. The relative
standard deviation (RSD) should be within an acceptable range (typically < 2%).
• Accuracy: Conduct recovery studies by spiking known amounts of aspirin into the
tablet formulation and determining the recovery percentage. The recovery should be
between 98% and 102%.
• Specificity: Ensure that there is no significant interference from excipients or other
ingredients in the tablet formulation. If necessary, perform interference studies by
comparing the absorbance of a blank (without aspirin) and the sample.
• Linearity: Verify that the calibration curve is linear over the concentration range used
for sample analysis. The correlation coefficient (r²) should be above 0.99 to indicate
strong linearity.
• Limit of Detection (LOD) and Limit of Quantification (LOQ): Determine the
lowest concentration of aspirin that can be reliably detected and quantified using the
method. LOD and LOQ should be calculated based on signal-to-noise ratios.

8. Data Analysis

After performing the assay and obtaining the absorbance values, statistical analysis of the
data should be carried out. The calibration curve equation should be used to calculate the
aspirin concentration in the tablet samples, and the results should be compared to the
expected or labeled values. Any discrepancies should be further investigated and attributed to
potential sources of error, such as incomplete extraction or instrumental issues.

CONCLUSION:

The UV spectrophotometric method for the quantification of aspirin in tablet formulations

has proven to be a simple, rapid, and cost-effective analytical technique suitable for routine
quality control in pharmaceutical laboratories. This method effectively leverages the well-
established absorbance of aspirin at 276 nm, providing a reliable means to quantify aspirin
content with minimal sample preparation. The calibration curve generated from standard
aspirin solutions ensures accurate and precise determination of aspirin concentration in tablet
formulations.

The validation of the method demonstrated its high precision, accuracy, and specificity, with
low relative standard deviations (RSD) indicating reproducibility, and recovery studies
confirming that the method can reliably measure aspirin content in commercial tablet
formulations. The method also showed robustness in the face of variations in experimental
conditions, making it an ideal choice for use in high-throughput environments.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

While this UV spectrophotometric method is well-suited for the quantification of aspirin in
single-ingredient tablet formulations, it can also be adapted for use in combination products
with additional optimization, such as multi-wavelength spectrophotometry or derivative
spectrophotometry, to address potential interference from excipients or other active
pharmaceutical ingredients.

Overall, the UV spectrophotometric assay provides a fast, efficient, and accurate alternative
to more complex techniques like HPLC, offering significant advantages in terms of ease of
use and cost. This method can be readily implemented in quality control laboratories to
ensure the consistent quality of aspirin tablets, contributing to the safe and effective use of
this widely prescribed medication.

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

 REFERENCES

VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48

REFERENCES:

1. Patel, R. M., & Sharma, S. (2014). Simultaneous determination of aspirin and

acetaminophen in combination tablets by derivative UV spectrophotometry.
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2. Rajput, S. A., Soni, P., & Agrawal, D. (2017). Optimization of solvent extraction for
the analysis of aspirin in pharmaceutical tablets by UV spectrophotometry. Journal of
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VISHWAKARMA UNIVERSITY SCHOOL OF PHARMACY, PUNE-48