UNIVERSITY OF MADRAS

B.Sc. DEGREE PROGRAMME IN BIOTECHNOLOGY

SYLLABUS WITH EFFECT FROM 2023-2024

Core Practical IV- GENETIC ENGINEERING AND r-DNA TECHNOLOGY AND

BIOINFORMATICS AND BIOSTATISTICS

Instructional Marks
Subject Code L T P S Credits
Hours CIA External Total
223C41 4 4 40 60 100

Learning Objective

LO1 Isolate the Plasmid DNA and Genomic DNA and predict the molecular weight
of DNA by agarose gel electrophoresis.

LO2 Demonstrate working principles of PCR, RFLP and other important Genetic
Engineering techniques.

LO3 Prepare the competent cells and perform bacterial transformation.

LO4 Determine the restriction digestion of DNA

LO5 Determine the restriction fragment length polymorphism.

UNIT Contents No. of Hours

1 Isolation of genomic DNA 9

Isolation of plasmid DNA

II Isolation of RNA 9

III Production of competent cells for transformation 9

Bacterial transformation

IV Restriction Digestion of DNA 9

V Restriction Fragment Length Polymorphism(DEMO) 9

PCR(Demonstration)

Total 45

Text Books

1 Laboratory Manual for GENETIC ENGINEERING 1st Edition, Kindle Edition

by S. JOHN VENNISON (Author) 2009.
 UNIVERSITY OF MADRAS
B.Sc. DEGREE PROGRAMME IN BIOTECHNOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024

MAPPING WITH PROGRAMME OUTCOMES AND PROGRAMME SPECIFIC OUTCOME

PO1 PO2 PO3 PO4 PO5 PO6 PSO1 PSO2 PSO3

CLO1 3 3 3 3 3 3 3 3 3

CLO2 3 3 3 3 3 3 3 3 3

CLO3 3 3 3 3 2 3 3 3 3

CLO4 3 3 3 2 3 2 3 3 2

CLO5 3 3 3 3 3 3 3 2 3

TOTAL 15 15 15 14 14 14 15 14 14

AVERAGE 3 3 3 2.8 2.8 2.8 3 2.8 2.8

Learning Objective

LO1 Analyse the Biological databases

LO2 Able to perform BLAST and FASTA

LO3 Represent data in to graphical form

LO4 Test the level of significance of biological data and interpret the results.

LO5 Determine averages of the biological data

UNIT Contents No. of

Hours

I Biological databases (NCBI, Swissprot and PDB) 9

II BLAST FASTA 9

III Identification of functional domains in nucleotide binding proteins using a 9

domain analysis server like SMART
 UNIVERSITY OF MADRAS
B.Sc. DEGREE PROGRAMME IN BIOTECHNOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024

IV Preparation of bar diagram, line diagram and pie diagram using MS 9

EXCEL.

Calculation of Central tendency- mean, geometric mean, median using MS

EXCEL

V Calculation of dispersion – Mean deviation, quartile deviation and standard 9

deviation using MS EXCEL

Calculation of student’s t test using MS EXCEL

Total 45

Text Books

1 Pennington, S.R. and Punn, M.J. 2002.Proteomics: from protein sequence to

function. Viva books Pri. Ltd.

2 Maleolm and Goosfship. J. 2001. Genotype to phenotype, 2ndedition. Bios Scientific

Publishers Ltd

3 Misener, S. and Krawetz. S.A. 2000. Bioinformatics: Methods and Protocols.

Humana press.

4 Attwood, T.K. and Parry-Smith, D.J.1999. Introduction to Bioinformatics. Pearson

Education Asia.

5 Primrose, S.B. 1998. Principle of genome analysis. 2ndedition. Blackwell Science.

Reference Books

1 Durbin, R., Eddy, S., Krogh, A. and Mitchison, G. 1998. Biological sequence
analysis. Cambridge University Press.

2 Friedman, C.P. and Wyatt. J.C. 1997. Computers and Machine: Evaluation methods
in medicinal information. Springer-verlag, New York.
 UNIVERSITY OF MADRAS
B.Sc. DEGREE PROGRAMME IN BIOTECHNOLOGY
SYLLABUS WITH EFFECT FROM 2023-2024

Web Resources

1 Bishop, M.J. and Rawhings. C.J. 1997. DNA and protein sequence analysis: A
practical approach. Oxford University press. New press. Kolodne

2 Kolodner, R.M. 1997. Computer in Health care: Computerizing large integrated

health networks. Springer – Verlag, New York

MAPPING WITH PROGRAMME OUTCOMES AND PROGRAMME SPECIFIC OUTCOME

PO1 PO2 PO3 PO4 PO5 PO6 PSO1 PSO2 PSO3

CLO1 3 3 3 3 3 3 3 3 3

CLO2 3 3 3 3 3 3 3 3 3

CLO3 3 3 3 3 2 3 3 3 3

CLO4 3 3 3 2 3 2 3 3 2

CLO5 3 3 3 3 3 3 3 2 3

TOTAL 15 15 15 14 14 14 15 14 14

AVERAGE 3 3 3 3 2.8 2.8 3 2.8 2.8