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Hanging Drop Preparation

The document outlines a procedure for studying the morphology and motility of bacteria using a hanging drop technique. It details the materials needed, the steps for preparing the slide, and how to observe bacterial movement under a microscope. The objective is to determine the motility of bacteria for diagnostic purposes.

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Colour nucleus
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29 views3 pages

Hanging Drop Preparation

The document outlines a procedure for studying the morphology and motility of bacteria using a hanging drop technique. It details the materials needed, the steps for preparing the slide, and how to observe bacterial movement under a microscope. The objective is to determine the motility of bacteria for diagnostic purposes.

Uploaded by

Colour nucleus
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Aim

To study the morphology and motility of bacteria in the given suspension.

Requirements
examined.
cavity slide, coverslip, vaseline, microscope and the bacterial suspension to be
A

Procedure

Vaseline is applied to the four corners of a clean coverslip.


coverslip.
Using a sterile loop,a loopful of the given suspension is placed on the center of the
center of the cavity.
Acavity slide is inverted over the cover slip so that the drop of suspension is in the
into the
The slide is quickly and carefully turned over so that the coverslip is on the top with the drop hanging
cavity.
mirror.
The microscope is adjusted for reduced light by lowering the condenser, and using the concave
observe the
The edge of the drop is focussed under low power. The microscope is then turned to high power to
morphology and motility of the bacteria in the given suspension.
Objective
To do amotility preparation from a purebroth culture of
bacteria for seeing the motility of bacteria for
the diagnosis of bacterial infections.
Motility Preparation
"Take a loopful of broth provided on a coverslip.
"Invert a microscopicslide with a ring of plasticine on the coverslip.
" Turn the arrangement so that the drop now hangs from the coverslip.
Focus the edgeof the drop under 10X objective of light microscope.
"Look at the movement of coccior bacilli with respect to brownian motion at 40X objective.
"Record your finding.
Report: Motile/nonmotile cocci/bacilli are seen.
Hanging Drop Technique for Bacterial Motility Examination

Vaselinel plasticine

Concavity

Inoculation
Slide
loop

Drop of
microbiological
culture

Coverslip

Drop
A
Cover glass

Depressed slide inverted


over the drop

The drop hanging when


the slide reversed

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