Microscope
Microscope
3BSC-5D
Forensic 3 (Forensic Chemistry and Toxicology)
Parts
Arm - Connects ocular tube and base. It also helps carry the microscope.
Base- Provides support to help microscope stand upright.
Coarse adjustment knobs - Focus of image under high power and moving the stage up and down.
Condenser - Forming a cone of all the dispersed light rays from the illuminator.
Diaphragm (Iris) - Controls the intensity of illuminating light.
Eyepiece (ocular lens) - Magnification of image produced by objective lens.
Fine adjustment knobs - Focus the image when viewing under high power.
Illuminator - Provides high-intensity light at the field aperture.
Mirror - Reflects light from an external source.
Objective lens - Primary magnifier of microscope.
Ocular tube - Maintains the correct distance between the ocular and objective lens.
Revolving nose piece - Holds the objective lens. Its rotation helps to change the power of the objective lens.
Stage - Place for holding sample.
Stage clips - Keeps the slide with a specimen in place on the stage.
Before we start to use a microscope, we should familiarize ourselves with the parts of the microscope and
get a good idea of where everything is and what everything does especially any dials or other interactive
features. This is best done while standing in front of the microscope where you would normally use it, but you
should also do a walk or see around to view any features that are hidden from view when using the device. We
should prepare microscope slides and should include a cover slip plastic or glass, depending on your purpose
and budget. If the objective lens should touch the slide which should not happen anyway if the device is used
correctly, see below, these covers will prevent or minimize damage caused. The stage clips should be used to
secure the slide and cover to the stage. Pushing down in the back end of these stage clips will open them. To
use a microscope, we have nine (9) steps before that what exactly is a microscope? Microscope is an optical
instrument consisting of a lens or combination of lenses for making enlarged images of minute objects and has
multiple lenses: the objective lens (typically 4x, 10x, 40x or 100x) is compounded (multiplied) by the eyepiece
lens (typically 10x) to obtain a high magnification of 40x, 100x, 400x and 1000x. Higher magnification is
achieved by using two lenses rather than just a single magnifying lens. To use it this the steps:
1. Turn the Objective Lenses so that the longest lens (the lowest power one) is in viewing position. Be sure that
there is room for it to move into place. Lower the Mechanical stage to make more room if needed.
2. Place the slide on the Mechanical stage and fasten it with the stage clips.
3. Look at the side of the microscope and turn the Adjustment knob until the lens is very close to, but not
touching, the slide.
4. Look through the eyepiece and move the Adjustment knob so that the lens lifts away from the slide. The
image should come into focus. Be careful not to drop the lens into the face of the slide, as this may cause
damage to the lens.
5. The condenser can be adjusted to increase or decrease light intensity. You will usually want the most light
possible for clearer viewing, but with low power objective lenses you may need to decrease the light.
6. The slide can be moved around to center the desired image in the field of view.
7. Once you have a clear image with the low-power objective, you may want to switch to a higher power one by
switching the objective lens. Because they are shorter, you don’t need to worry about turning them into the face
of the slide and causing damage. This is one of the reasons we start with the lower ones.
8. You may need to slightly adjust the focus and centering of the object you are viewing. If you try this, and it
doesn’t seem to come into focus, then drop the lens to very near, but not touching, the slide (look from beside
the microscope to do this, not through the eye piece) and then look through the eyepiece while you slowly raise
the lens away from the slide. At some point, it will come into focus for you.
And last 9. When you have finished viewing the slide, lower the Mechanical stage using the Adjustment knob,
click the low power lens into viewing position (in preparation for next time), and remove the slide (by pressing
on the ends of the clips to release it).