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Sds Page Tables

The document provides detailed formulations for running and stacking gels used in electrophoresis, including the specific volumes of water, Tris-HCl, acrylamide, bis-acrylamide, SDS, APS, and TEMED for various concentrations. It outlines the total volume for each gel type and emphasizes the use of fresh APS at a concentration of 100mg per 1mL of solution. The data is presented in a tabular format for easy reference.

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14 views1 page

Sds Page Tables

The document provides detailed formulations for running and stacking gels used in electrophoresis, including the specific volumes of water, Tris-HCl, acrylamide, bis-acrylamide, SDS, APS, and TEMED for various concentrations. It outlines the total volume for each gel type and emphasizes the use of fresh APS at a concentration of 100mg per 1mL of solution. The data is presented in a tabular format for easy reference.

Uploaded by

sofiademoraisguedes
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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Running Gel

5% 7.5% 10% 12.5% 15% 20%


H2O 13.7 27.3 54.6 11.3 22.6 45.2 9.0 17.9 35.7 6.6 13.2 26.3 4.2 8.4 16.9 - - -
Tris-HCl 1.5M pH 8.8 6.25 12.5 25.0 6.25 12.5 25.0 6.25 12.5 25.0 6.25 12.5 25.0 6.25 12.5 25.0 6.25 12.5 25.0
Acrylamide 40% 3.04 6.07 12.14 8.10 9.11 18.22 6.08 12.15 24.3 7.60 15.19 30.38 9.12 18.23 36.46 12.15 24.3 48.6
Bis-Acrylamide 2% 1.68 3.35 6.7 4.47 5.02 10.04 3.35 6.7 13.4 4.18 8.37 16.74 5.03 10.05 20.1 6.70 13.4 26.8
SDS 10% 0.25 0.5 1.0 0.25 0.5 1.0 0.25 0.5 1.0 0.25 0.5 1.0 0.25 0.5 1.0 0.25 0.5 1.0
APS 10% 0.125 0.25 0.5 0.125 0.25 0.5 0.125 0.25 0.5 0.125 0.25 0.5 0.125 0.25 0.5 0.125 0.25 0.5
TEMED 0.015 0.03 0.05 0.015 0.03 0.05 0.015 0.03 0.05 0.015 0.03 0.05 0.015 0.03 0.05 0.015 0.03 0.05
total volume (mL) 25 50 100 25 50 100 25 50 100 25 50 100 25 50 100 25 50 100

Note: Fresh APS. 100mg per 1mL of solution.

Stacking Gel

4%
H2O 11.6 3.87
Tris-HCl 1.5M pH 8.8 5.04 1.68
Acrylamide 40% 1.94 0.647
Bis-Acrylamide 2% 1.07 0.357
SDS 10% 0.20 0.067
APS 10% 0.11 0.037
TEMED 0.02 0.007
total volume (mL) 20 6.7

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