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Medical Microbiology Lab Report Members

The document is an internship report from Arba Minch University detailing microbiological testing procedures and results. It outlines objectives, materials, and methods for media preparation, sample collection, colony observation, Gram staining, biochemical identification, and drug susceptibility testing. The report concludes that the laboratory tests successfully identified bacterial isolates, aiding in the selection of appropriate antibiotic therapy.

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Bisrat Bogale
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28 views6 pages

Medical Microbiology Lab Report Members

The document is an internship report from Arba Minch University detailing microbiological testing procedures and results. It outlines objectives, materials, and methods for media preparation, sample collection, colony observation, Gram staining, biochemical identification, and drug susceptibility testing. The report concludes that the laboratory tests successfully identified bacterial isolates, aiding in the selection of appropriate antibiotic therapy.

Uploaded by

Bisrat Bogale
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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ARBAMINCH UNIVERSITY

COLLAGUE MEDICENE AND HEALTH SCEINCE

DEPARTMENT OF MEDICAL LABORATORY

Internship attachment; Micro biological test report

Name ID

Wassie Enyew NSR/2394/13

Bisrat Bogale NSR/642/13


Submittion to Mr. Sleshi B.

Submitted date 26/5/2017 E.C

Contents

Laboratory Report: Microbiology Testing.......................................................................................2


Objective:...................................................................................................................................2
Materials and Methods:.............................................................................................................2
1. Media Preparation..............................................................................................................2
2. Sample Collection and Inoculation......................................................................................2
3. Colony Morphology Observation........................................................................................2
4. Gram Staining and Microscopic Examination......................................................................2
5. Biochemical Identification Tests.........................................................................................2
6. Drug Susceptibility Testing (Antibiotic Sensitivity Test)......................................................2
Results and Discussion:...............................................................................................................2
Conclusion:.................................................................................................................................2
References:.................................................................................................................................2
Laboratory Report: Microbiology Testing
Objective:
To perform microbiological testing, starting from media preparation to bacterial
identification and drug susceptibility testing, ensuring accurate diagnosis and treatment
of infectious diseases.

Materials and Methods:


1. Media Preparation
Objective: To prepare culture media suitable for microbial growth.

Materials:
- Nutrient Agar (NA), MacConkey Agar, Blood Agar, Mueller-Hinton Agar, and other
selective/differential media
- Distilled water
- Sterile Petri dishes and test tubes
- Autoclave
- Weighing balance
- Measuring cylinder

Procedure:
1. Measure the required amount of media powder and dissolve it in distilled water as
per manufacturer instructions.
2. Adjust the pH if necessary.
3. Autoclave the media at 121°C for 15 minutes to sterilize.
4. Allow the media to cool to about 45-50°C before pouring it into sterile Petri dishes or
test tubes.
5. Let the media solidify at room temperature before use.

2. Sample Collection and Inoculation


Objective: To collect and inoculate clinical samples for microbial culture.
Materials:
- Sterile swabs, syringes, or blood collection tubes
- Culture media plates and broth
- Bunsen burner
- Inoculating loop

Procedure:
1. Collect samples (e.g., blood, urine, pus, throat swab, sputum) using sterile techniques.
2. Streak the sample onto appropriate culture media using the quadrant streak method
to isolate colonies.
3. Incubate at 37°C for 18-24 hours under appropriate conditions (aerobic or anaerobic).

3. Colony Morphology Observation


Objective: To observe bacterial growth characteristics.

Procedure:
1. Examine colony size, shape, color, and hemolytic pattern (on Blood Agar).
2. Record whether colonies appear mucoid, dry, or irregular.
3. Subculture representative colonies for further testing if needed.

4. Gram Staining and Microscopic Examination


Objective: To differentiate bacteria based on cell wall composition.

Materials:
- Crystal violet, iodine, ethanol, safranin
- Glass slides
- Microscope
- Immersion oil

Procedure:
1. Prepare a bacterial smear on a glass slide and heat-fix.
2. Stain with crystal violet for 1 minute and rinse.
3. Add iodine for 1 minute and rinse.
4. Decolorize with ethanol (few seconds) and rinse immediately.
5. Counterstain with safranin for 30 seconds, rinse, and air dry.
6. Observe under the microscope using oil immersion.
7. Record results: Gram-positive (purple) or Gram-negative (pink).

5. Biochemical Identification Tests


Objective: To identify bacterial species using biochemical reactions.
Common Tests:
- Catalase Test
- Coagulase Test
- Oxidase Test
- Triple Sugar Iron (TSI) Test
- Urease Test
- Indole, Methyl Red, Voges-Proskauer, Citrate (IMViC) Test

6. Drug Susceptibility Testing (Antibiotic Sensitivity Test)


Objective: To determine the effectiveness of antibiotics against the bacterial isolate.

Materials:
- Mueller-Hinton Agar
- Antibiotic discs (e.g., penicillin, ciprofloxacin, amoxicillin, tetracycline)
- Sterile swab
- Incubator
- Ruler or caliper

Procedure (Kirby-Bauer Disk Diffusion Method):


1. Prepare a bacterial suspension in saline to match 0.5 McFarland standard.
2. Using a sterile swab, evenly spread the suspension over Mueller-Hinton Agar.
3. Place antibiotic discs onto the agar surface using sterile forceps.
4. Incubate at 37°C for 18-24 hours.
5. Measure the zone of inhibition around each disc using a ruler.
6. Compare results with CLSI (Clinical and Laboratory Standards Institute) guidelines to
determine resistance or susceptibility.

Results and Discussion:


- Describe bacterial colony morphology and Gram stain results.
- Record biochemical test results and compare with identification charts.
- Interpret antibiotic susceptibility results (resistant, intermediate, or susceptible).
- Discuss the clinical significance of the isolated bacteria and their antibiotic resistance
pattern.

Conclusion:
This laboratory test successfully identified bacterial isolates through morphological,
biochemical, and antibiotic susceptibility testing. The findings help guide appropriate
antibiotic therapy for effective patient management.
References:
- Clinical and Laboratory Standards Institute (CLSI) Guidelines
- Standard Microbiology Laboratory Manuals

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