January 12th, 2016

API-4000
Troubleshooting
Guide

Page 1 of 25
Contents
Interface Heater Problem: No catastrophic error message 3

Gas Control Schematic 3

Heater Test Procedure Interface, Turbo Heater, APCI Heater 4

Gas Flow Leak Test Procedure 5

Heater Troubleshooting by Laurie Styles 6, 7, 8

Block Diagram of the Temperature Controller 9

Block Diagram of Heater Power Distribution 10

APCI Source Attached - F3 Fuse: Appendix A 11

Curtain Plate stuck to Orifice Plate: Appendix B 11

Turbo Ion Spray probe recognized as Ion Spray probe: Appendix C 11

Heater Run Away: Cracked Ceramic Tube: Appendix D 12, 13

59 Multiple Peaks & Renin low sensitivity: Appendix E 14, 15

CAD Assembly Versions WC02-6397: Appendix F 16

Setting the CAD Gas Restrictor Valve: Appendix G 17

TW700 Serial Numbers with Axial Damping Upgrade: Appendix H 18

Do not replace Electrode Tube with source attached: Appendix I 19

Curtain Gas over 20; high masses lose sensitivity: Appendix J 19

Oil Return Kit install instructions: Appendix K 20 – 23

Sample tubing too short: Appendix L 24

Dimple TIS: Appendix M 25

Page 2 of 25
Interface Heater Problem: No catastrophic error message
Must Read!
For sensitivity problems be sure to check this first.

Problem:
This is only a problem if the interface heater is defective!
This problem describes a defective Interface Heater.
In the Method the ih (interface heater) was toggled from "Off" to "On".
The Status went from "Not Ready" to "Off" after a few minutes.
Should have went to "Ready" if it was not defective.
This should have been a catastrophic error. Status "Red" not allowing you to start a run.
But it was not and the status stayed "Green". Allowing you to start a run.
Unless you actually look to see the Interface Heater "Off" in the status window
you don't know it's defective.
A customer may run for a long time without knowing they have an Interface Heater failure and lose out on
increased sensitivity.

Solution: This should be fixed in Analyst 1.4.

Gas Control Schematic

Page 3 of 25
Heater Test Procedure
Interface Heater Test: (Orifice Plate P/N WC02-7956)
 Toggle the Interface Heater (ihe) button in Analyst Acquisition Method to “ON” position.
 Open Mass Spec Detailed Status and look for Interface Heater status to indicate “Ready”.

Turbo Heater Test:

 From Analyst Method set Gas 2 = 40 and Temp = 300° C.
 Look for “Temperature Reached” message in the “source/gas” tab of the
Analyst Acquisition method or disappearance of “Not at set-point” message near the
“Source Temperature (deg. C)” in the Mass Spec Detailed Status.
 Increment by 50° C up to 600° C checking Temperature in the Mass Spec Detailed Status or in the
Acquisition Method after each temperature increase.
 After 600° C you should observe both heaters to be glowing equally bright.
 Increment by 50° C up to 750° C. See Technical Note.
 Check Status after each temperature increase.
 Ensure the source cooling fan located on the top of the front bulkhead cover is operational when the source
housing becomes hot. Fan turns on at 60C  5.

APCI Heater Test: (P/N WC02-8102 Heater FRU)

 Install the APCI probe. (Corona discharge needle FRU P/N WC02-7947)
 From Analyst Method set Temp = 300° C and Gas 1 = 20.
(aside: there’s no gas 2 or aux for APCI)
 Look for “Temperature Reached” message in the “source/gas” tab of the
Analyst Acquisition Method or disappearance of “Not at set-point” message near
the “Source Temperature (deg. C)” in the Mass Spec Detailed Status.
 Increment Temp by 50° C.
 Check the temperature status in the Mass Spec Detailed Status or in the Acquisition Method.
 After 600° C you should not observe glowing but source stack should feel hot to the touch.
(aside: for APCI only the stack gets hot not the housing and vice versa for Turbo).
 Increment by 50° C up to 750° C . See Technical.
 Check Status after each temperature increase.

If one Heater is brighter than the other you may have a gas leak or a faulty heater.
Carefully inspect the heater end caps for dislodged ceramic balls from inside heaters.
Also check the bottom of the source housing for evidence of ceramic balls falling out of heaters.
Perform the gas leak test. (see below)

Technical Note:
If the Turbo or APCI heaters are normally not operated above 600° C response time could be affected.
When incrementing above 600° C by steps of 50° C the temperature may take longer than 10 minutes to
reach set point and time out (electronics turn off). This is not a problem.
You will need to decrease the temperature increment (anywhere from 5° C to 25° C) to complete the test.

Test Points:
Sensor 50   15% as measured on the 24 pin connector on the source housing.
Heater 8.5   15% as measured on the 24 pin connector on the source housing.

Turbo Test Points: Heater1: pins 10 & 22 Heater2: pins 11 & 23 Sensor: pins 5 & 17
APCI Test Points: Heater: pins 9 & 12 Sensor: pins 7 & 8.

Page 4 of 25
Gas Flow Leak Test Procedure
Gas 2
Gas Flow Leak Test: Gas 1 (Neb)
Bath Gas
 Leak test using 50/50 methanol/water or
Gas 2
equivalent on the following gas connections.
 Look for micro bubbles and repair leak if detected.

Gas 1 (Neb)
Leak test gas connections located at:
1. GAS 1 line + (2 x GAS 2) lines for both
turbo heaters + Bath Gas line connections
on the interface flange. A total of 4 connections.
2. Junction between the interface flange and Bath Gas
source housing.
3. Black Gas 1 and Bath Gas tube connections
on the source stack.
If leaks are found and cannot be repaired
replace the whole assembly.
Order: Kit Interface FRU P/N WC02-7954

Gas 1 (Neb)

Bath Gas

Gas 2 “T” splitter

Gas Controller

Page 5 of 25
Heater Troubleshooting by Laurie Styles
To address the issue of the heaters not reaching the temperature, I’d like to suggest some troubleshooting
techniques and tips (for engineers) that we discussed and used during API 4000 training in April.
The interface heater can easily be checked by first removing the orifice plate and ohming
(measuring the resistance) of the heating and the sensing element.

Pin # 1

Remove curtain plate Check pins on orifice plate

a. All pins to their adjacent pins Open circuit (infinite ohm)
b. Pin 1 (from bottom) to pin 5 45 ~ 55 ohm
c. Pin 2 to pin 6 36 ~ 44 ohm

If the above measurements are within 10-15% of the indicated values, the heater and the
sensor are functional.
If not, the replacement API 4000 orifice plate part # is WC02-7956
Similar measurements can be done for the APCI and the TIS heaters, but with even less
effort. All measurements can be done through the 24-pin connector on the source
housing after it has been removed from the instrument. No special tools (other than a
digital multi meter, DVM) or dismantling of the source is required. The type of probe
(APCI or TIS) installed in the source housing is irrelevant for this measurement.

Page 6 of 25
Heater Checks - 24 pin connector

Remove Source

TIS/APCI ID
TIS 1.45 KΩ
APCI 0.16 Ω

24 pin connector

Pin out Specification

a. Pin 5, 7, 9, 10, 11, 12 to chassis of housing Open circuit (infinite ohm)
b. Pin 5 and 6, pin 5 and 17 45 ~ 55 ohm
c. Pin 7 and 8, pin 7 and 18 45 ~ 55 ohm
d. Pin 9 and 12 7.2 ~ 8.8 ohm
e. Pin 10 and 22 7.2 ~ 8.8 ohm
f. Pin 11 and 23 7.2 ~ 8.8 ohm
Again, if all of the measurements are within 10-15% of the above-indicated values,
the APCI and TIS heaters are functional.
If not, the replacement Turbo Heater FRU kit part # is WC02-7964 old should perform upgrade
And the replacement APCI Heater FRU kit part # is WC02-8102

NOTE:
There should be no short between any of the pins and the source housing
(impedance must be infinite). Any measurable impedance between the source housing
and any of the pins in the 24-pin connector indicates improper installation of the heaters.
If it is not corrected, such short will likely cause a heater and/or temperature control board failure.
Care must be taken not to pinch or crimp GAS 2 lines while assembly since the TIS
heaters will exhibit different heating characteristics (glow with different intensities). This
failure mode may also cause TIS heaters to fail.

Page 7 of 25
The following is also taken from API 4000 training material.
There are 5 feedback pins, see figure below, on the temperature control board (TCB)
that are accessible only when the back cover is removed.
IHE (on analyst) TP2 TP1
OFF 0.0+/-0.05Vdc 0.0Vdc
ON 1.0+/-0.05Vdc 1.0V oscillates ± 0.05 V
Tower Sensor: 110 – 140 ºC
TEM (on analyst) TP4 TP3 Cooling Fan: 60 ºC ± 5
500 500x8.58mV/degC 4.29V oscillate (4.29Vdc)

All test points (TP1 through TP4) are referenced to a ground potential (i.e., TP5).
Note: API 4000 The Curtain Plate voltage for the Lens P/S is:
For TIS: 550 +/- 75 V For D29 & D30
For HN: 1100 +/- 150 V RED LED : Not at set point.
GREEN LED : At set point

D30 Interface Heater LED

D29 APCI & Turbo Heater LED

TP 5 = ground
TP 4 = Turbo / APCI Heater set point
TP 3 = Turbo / APCI Heater read back
TP 2 = ih set point
TP 1 = ih read back
Note: New Temp Control PCB P/N 1032788
TP1 & 2: ih read back and set point voltages
ramp to prevent ceramic heater failure.

Interface Heater LED

D19 is normally off; if it's red, that
means either the Housing is not
there or the machine is in fault
condition. To reset D19, just remove
the housing and connect it back to
the machine. If problem persists,
trouble-shooting is required.
5-7 minute delay for D19 to go red.

APCI & Turbo LED

D17 is normally off; if it's red, that
means either the Probe is not there
or the machine is in fault condition.
To reset D17, just remove the Probe
and connect it back to the housing.
If problem persists, trouble-shooting
is required.
5-7 minute delay for D17 to go red.

If the read-back signals oscillate approximately 15°C above and below the set point, there
is an indication of a faulty temperature control board.
Temperature Control Board PCB part #: WC02-5551
I hope this gives some troubleshooting tips to isolate the problem. I will be in contact
with the service engineers on site with some more specifics.
Laurie Styles

Page 8 of 25
Block Diagram of the
Temperature Controller Connector J5
from Heater transformer
Pin # Signal name
1 signal GND
2 -16Vac
3 +16Vac
4 CH GND
5 AC GND
6 95_Vac

Heater
Transformer J5
Interface
J1 Assembly

Temperature
Controller
DACs and PCB Ion Source
Vacuum J4 J2
Gauge PCB
J3

System
Controller Connector J2 (from Ion source)
Module Pin # Signal name
3 GROUND
4 SOURCE-ID
With Power OFF
measure between Pin 3 & 4
TIS APCI NONE
1.47K 0 ohm open

Page 9 of 25
Block Diagram of Heater Power Distribution

Connector J5
from Heater transformer
From (LAMBDA) Switching Power Pin # Signal name
Supply 1 signal GND
AC DISTRIBUTION BOARD 2 -16Vac
3 +16Vac
4 CH GND
AC 5 AC GND
F3 6 95_Vac
4Amp
95 Vac

POWER
HEATER TRANSFORMER +/- 16Vac

+5 V DC
CONTROL
VOLTAGE REGULATOR
+/- 15V DC

COMPARATOR +/- 2.5 V Ref

VOLTAGE REFERENCE

Page 10 of 25
Appendix A:
APCI Source Attached - F3 Fuse
Always fault in Status Log no matter if source is attached or not.

Problem:
Always get message "APCI" source attached even if it's "TIS" or no source installed.

Solution: new Long Delay 4Amp fuse Part Number 10003263

F3 Fuse blown. F3 P/N WC03-0352 FUSE 4A 250V 2X20 SLO BLO
Located next to power buttons near switching power supply,
2.5 amp fuse slow blow in consumables kit.
Causes loss of power to heater board therefore status frozen to APCI status. This Screw too deep shorts pins.
Error is wrong source attached.
Note:
This problem can be intermittent and may require replacing fuse a couple of times.
Replacing the source has fixed this problem. Usually happens on power up.

Appendix B:
Curtain Plate stuck to Orifice Plate
1) We've had reports of the curtain plate stuck to the orifice plate.
In some instances they would not come apart and therefore both needed to be replaced.
Some engineers have had success unscrewing the curtain plate like a cap while pulling on it.

2) Also some plates are on so tight that when trying to remove the plate
the orifice plate assembly pulls out from the vacuum chamber thus creating a vacuum leak.
You hear a vacuum hiss and the turbos may start to change speeds.
 Orifice Plate P/N WC02-7956
 Curtain Plate P/N WC02-6218

Appendix C:
Turbo Ion Spray probe recognized as Ion Spray probe
by Dave Emplit
Recently I had a 4000 report the Turbo Ion Spray probe as an Ion Spray probe in the detailed status box.
This was accompanied by the disappearance of the "Temp" setting in all methods.
Changing to the Heated Neb. probe, I found it was properly identified and worked OK.
After trying a different Turbo Spray probe and finding it was also correctly identified and worked OK, I took a
closer look at the little circuit board on the suspect probe.
I found a cold solder joint at the resistor on the bad probe causing the impedance
to vary between 2K-10K ohms.
The good Turbo Spray source had an impedance of about 1.5K ohms.
After carefully re-soldering the resistor chip on the board the impedance was then stabilized at 1.4K ohms.
The probe was then correctly recognized as a Turbo Ion Spray and all was well.

Page 11 of 25
Appendix D:
Heater Run Away: Cracked Ceramic Tube
FRU (Field Replacement Unit) P/N WC02-7947
Symptoms:
1. Interface Heater goes from Ready to Off after a few seconds.
Also when you put the source on you may hear clicking from the heater board.
2. One or Both Turbo Heaters always “Glowing Red”.
Ion Spray voltage “ON” and temperature is set to “0”.

Ceramic Tube

Cracked ceramic tube

APCI Corona
Discharge Needle
Turbo Heaters
Solution:
This problem can be caused by a cracked APCI corona discharge needle ceramic tube.
If the tube cracks the ion spray voltage leaks through the crack and back to the circuit boards inducing the
heater run away condition.
The turbo ion spray probe and the APCI corona discharge needle are tied together.
They both have ion spray voltage even though the APCI probe is not installed.

Phone Fix & Temporary Solution:

If a customer has this symptom ask them to make the ion spray voltage “0” and the temperature “0”.
If the APCI probe is installed make the NC (Nebulizer Current) “0”.
If the problem is the cracked tube the heaters will turn off.
The temporary solution is to remove the APCI corona discharge needle.
Do this by holding the needle at the pointed end and unscrewing the positioning nut at the cap end.
The positioning nut is used to swing the needle around.
The needle comes out the bottom of the source through the exhaust hole.

Permanent Solution: Replace the Ceramic Tube

As many as 30 of the earlier sources used a set screw to hold the ceramic tube in place. Over tightening this
screw caused the ceramic tube to crack. The fix for this is to order the ceramic tube FRU (Field Replacement
Unit) P/N WC02-7947 which removes the set screw and replaces it with a clip.

Without Upgrade With Upgrade

No Clip with set screw Clip WC02-8096 with set screw removed

Set Screw Set Screw has been removed

Page 12 of 25
 Ceramic Tube FRU: P/N WC02-7947
The FRU instructs you to remove the set screw and install a clip.
Kit includes:

1. Nut P/N WC02-8097 APCI

2. Spring P/N WC02-7013
3. Ceramic Tube P/N WC02-7455
4. Needle P/N WC02-7459 (Corona Discharge)
5. Gold Spring P/N WC02-7497 (For blue wire)
6. Allen Key 0.9 mm P/N WC02-8105 used for 2mm set screw
7. Conical (Clip) P/N WC02-8096 (Spring that needs cutting)
8. Set Screw 2mm P/N WC02-5393
Miscellaneous Part Numbers:
1. APCI Nut P/N WC02-7458
2. HV Cable (Blue Wire) P/N WC02-5721

Ceramic Tube replacement:

See Instructions included with FRU for more details.
Remove the TIS or APCI probe.
Remove the blue wire (Ion Spray High Voltage Cable) by unscrewing the black nut.
Remove the set screw shown in picture and disconnect both gas lines.
The top assembly slides out far enough to allow for ceramic tube replacement.

Remove the nebulizer gas black tube.

Remove the blue wire (Ion Spray High Voltage Cable)

by unscrewing the black ferrule.

You should now have access to the ceramic tube.

If you find it necessary to remove the electrical wires see
FRU instructions.

Remove the bath gas black tube.

Page 13 of 25
Appendix E:
59 Multiple Peaks & Renin low sensitivity
Problem:
During API-4000 installation 59 shows multiple peaks.
Usually goes away after a few days. Sometimes it does not.
Renin produces a very strong 910 peak reducing 880 intensity by 70 to 80%. See page 2 for full report.

59 Multiple Peaks (not correct) 59 One Peak (Correct)

Solution: Source of problem found to be contaminated Needle

Solution:
Replace the syringe, the needle, the peak tubing and
most importantly the “Turbo electrode tube P/N WC02-5392”.

Page 14 of 25
Why Hamilton Syringes are causing problems by Jesse Hines
I just finally figured out exactly why the Hamilton Syringe that we supply with the instrument is so bad. Recall
that this is the 1ml syringe with the leur fitting. The inside of the leur is not chrome plated and is not made of
stainless steel yet is in contact with the liquid inside the syringe. The syringe I was using at the Medical
University of South Carolina was actually ruining my Renin standard by adding copper to my standard! This
resulted in a very strong 910 peak reducing my 880 intensity by 70 to 80%!!

By turning up the orifice to 120 I was able to observe the singly charged 1758.93 peak and an 1821 peak above
that! I used the relative intensities of the isotopes of 1758.93 and constructed the expected isotopic distribution
for 1821 using the known abundances of Cu63 and Cu65 and was able to predict the distribution! (I am
attaching my excel spreadsheet with this reconstruction) No doubt that this is copper!

Certainly makes sense because the base metal of the leur is not stainless but is chrome plated. It is common in
chrome plating to plate copper and/or nickel first then the chrome to aid in adhesion. (I used to run a plating and
coating facility.) Chrome won't stick without it. The reason there is no chrome on the inside is because the bath
used to plate the chrome relies on an electrical current passing through the bath to the part to cause the chrome
to deposit. (Thus the name electroplating!) The inside of the leur is a field free area (similar to a faraday cage)
and does not receive any chrome. This is a common problem in plating parts. Inside corners don't plate either!
The copper was most likely applied in an electro less bath that plates copper to any exposed surface because it
is essentially a supersaturated solution of copper. It doesn't need the electric field to deposit the copper so it
gets on the inside.

When our standard, PPG's or Renin, are placed inside the syringe the difference in the chemical potential of the
two different metals causes the copper to go into solution! It binds to our standard and reduces the intensity of
the standard peaks that we are using to test the instrument during installs! Makes installs really tough! Roger is
familiar with some strange behavior of the PPG 59 amu peak at the CDC during the API4000 installs. The
electrolytic action inside the syringe breaks down the samples in the syringe too!
Get rid of this syringe! Hamilton has many other types of syringes that work well!
Thanks,
Jesse Hines

Page 15 of 25
Appendix F:
CAD Assembly Versions WC02-6397.
This is not a stocked part. Must special order from Sciex. You will receive improved design.
This assembly WC02-6397 includes everything. Block, restrictors, tubing .....
The API-4000 has been shipping with this new improved CAD Assembly.
The replacement part number is the same as old part number and directly replaces the older version.
This version improves potential leak points found in original design.
The API-4000 Future Design is to accommodate the "new turbo pump" the API-4000 will ship with in July.
The Leybold TW700 will be replaced by the Varian TV801.

Page 16 of 25
Appendix G:
Setting the CAD Gas Restrictor Valve
API 4000 Varian Pumps “S” series single rough pump
Summary
A CAD gas restrictor valve has been introduced to adjust and standardize the CAD gas pressure.

Cap covers valves

Setting the CAD Gas Restrictor Valve

On instruments that ship with a single roughing pump, a restrictor valve is located on the inlet line to the
Solenoid Gas Flow Controller. The CAD gas restrictor valve compensates for a higher CAD gas source
pressure in the single pump configuration and ensures that instrument-to-instrument variations in CAD gas
response are minimized. The restrictor is pre-set at the factory and a locking device is used to prevent
accidental adjustment or tampering. Under routine operation, the valve should not require adjustment in the
field. If the roughing pump is replaced, the CAD Gas Restrictor should not need to be adjusted. However, it is
advisable that the valve be set to ensure that a pressure between 4.6 x 10-5 and 5.5 x 10-5 Torr can be
achieved at a CAD gas setting of 12. If an interface, or interface component (for example, an orifice plate or a
skimmer cone), has been replaced, the CAD Gas Restrictor may require adjustment. The procedure for setting
the CAD Gas Restrictor is described below.

Procedure
The filament voltage can be read from test point PRE on the DACs and VACs module.
Turn the CAD gas restrictor knob clockwise until the valve is fully closed.
1. Set the CAD Gas to 0.
2. Measure and record the base pressure (0.06 - 0.13).
** Make sure the base pressure is in this range (0.06 - 0.13) before proceeding**
3. Set the CAD Gas to 12.
4. Adjust the CAD Gas Restricter valve for a pressure of Base + (0.4 – 0.42).

Example: Base pressure = 0.09 volts

CAD pressure = Base + (0.4 – 0.42)
Calculate CAD 12 Range = 0.09 + (0.4 – 0.42) = 0.49 - 0.51 volts or 4.9 – 5.1 e-5 Torr.
Adjust CAD Gas Valve for a pressure between 0.49 - 0.51 volts or 4.9 – 5.1e-5 Torr.

CAD Pressure Specification (Volts) Calculate CAD 12 Range Record

CAD = 0 (Base) 0.06 - 0.13 Base = _____________
CAD = 12 Base + 0.4 _________+ 0.4 = ________
CAD = 12 Base + 0.42 _________+0.42 = ________
CAD 12 Range = Base (0.06 - 0.13) + (0.4 – 0.42)
CAD 12 Range = (0.46 – 0.48) – (0.53 – 0.55) = 0.46 - 0.55 volts or 4.6 – 5.5e-5 Torr

Page 17 of 25
Appendix H:
TW700 Serial Numbers with Axial Damping Upgrade

Caution: Be sure to choose the correct serial number Plate. There are two.
The Correct Plate is located on the side of the TW700.

TYP 700L-TDL
Ser. Nr. 20900406212
(This serial number and above have the Axial Damping Upgrade)
If any of these pumps fail be sure to notify Sciex immediately.
Do not use the plate on the back with
TYP Turbo Drive L.
Ser. Nr. XXXXXXX

According to SB 131, the red stickers are only for pumps that Leybold has
reworked after the pumps were returned and refurbished.
New pumps with the Axial dampening upgrade will not have this sticker.

For the pumps that have failed please send in the TW700 failure form and
have the pump returned to Leybold as quickly as possible for analysis.
Thanks
Rainer

Page 18 of 25
Appendix I:
Electrode Tube P/N WC02-5392
Do not replace Electrode Tube with source attached

Appendix J:
Curtain Gas over 20; high masses lose sensitivity
Curtain Gas > 20
High masses lose sensitivity

Note: Curtain gas set 15 – 20.

Masses above1200 amu lose sensitivity
and have choppy looking peaks.

When the curtain gas is set to 20 or higher

masses ~1000 amu or greater lose sensitivity.

Page 19 of 25
Appendix K:
Oil Return Kit P/N WC02-7583
Prevents oil overflow from exhaust port
Oil Return Kit P/N WC02-7583 includes
1) Oil return parts Only P/N WC02-8685 includes connector and tubing
2) Oil Mist Filter Only P/N WC02-8628
Illustrated Instructions

Page 20 of 25
 Remove
Ballast

Eye Glass

Remove
Plug

Screw in fitting with wrench

Notice: No Clearance

Page 21 of 25
 Elbow 90° P/N WC02-8689
used to clear top cover

Anti Suckback Valve on top of Oil Mist Filter

Reeds facing up

Ballast plug replaced with Oil Mist Filter Only

this connector P/N WC02-8628
Oil condenses inside
canister.

Tubing cut to length.

Oil return parts Only

P/N WC02-8685 Oil returns to pump by
includes connector and tubing way of ballast port.

Page 22 of 25
 To fume hood

Elbow 90° P/N WC02-8689

used to clear top cover

Note:
Do not put the Antisuckback valve on the rough pump side.
The Antisuckback valve will condense the oil on the reeds and oil leaking
will occur. The Antisuckback valve should be placed on the top of the oil
mist filter canister. The canister will collect the oil and return it to the
pump through the ballast port.

Page 23 of 25
Appendix L:
Sample tubing too short

Gray (Tan) tubing should be at least:

30 cm or 11.8 inches long
Gray (tan) tubing
WC01-6485 is 0.0025” ID
Red tubing
WC01-6316 is 0.0050” ID

If this tubing is too short the Ion Spray voltage will be shorted to ground.
Red or Gray tubing is OK to use so long as the length is 30 cm.

Page 24 of 25
Appendix M:
TIS Dimple

Electrode Tube
Sprayer Probe
Electrode Housing

Wedge (inside)

Curtain Plate Source window

Orifice

Dimple (outside)
marks area of wedge

An incorrectly indexed probe may still work well, but it may take more time to achieve
sensitivity at high flow rates (more than 200 ul/min). Indexing makes little or no difference for
low flow rates (infusion). If the probe is performing badly, or if the position of the 'sweet spot'
is critical, (ie the sensitivity drops rapidly with just a small change in probe position, then it this
may be because the dimple is in the wrong place.

Page 25 of 25