time X ln X

0 0.0022 -6.11929792
27.8 0.0022 -6.11929792
30.63 0.0314 -3.46094739
33.57 0.0503 -2.9897502
39.27 0.0839 -2.47812967
46.6 0.067 -2.70306266
48.97 0.0629 -2.76620912
54.03 0.0461 -3.07694233
60.73 0.0189 -3.96859336
64.87 0.0103 -4.57561138
65.97 0.0186 -3.9845937
67.07 0.0369 -3.29954373
68.67 0.0523 -2.95075891
71.2 0.0829 -2.49012022 μ=
89.25 0.2006 -1.6064424 tgen=
99 0.2217 -1.50643016 tlag=
114.8 0.3457 -1.06218393
137.25 0.6172 -0.48256216
147.33 0.6658 -0.40676595
160.92 0.8634 -0.1468772
170.33 1.1504 0.14010971
185.33 1.5501 0.43831944
192 1.7977 0.58650807
218.67 2.5201 0.92429858
243.5 2.0155 0.7008673
258.33 1.9421 0.66376986
 ln X vs time
2

f(x) = 0.0235699467202405 x − 3.8766

0 R² = 0.981761946767646
0 50 100 150

-1

0.02356995 h-1 -2
ln X

29.4080928 h
70 h -3

-4

-5

-6

-7

time
 ln X vs time

= 0.0235699467202405 x − 3.87662256445948
0.981761946767646
100 150 200 250 300

time
 A X
X vs A
0.059 10
0.141 25 120
0.272 50
100
0.553 100 f(x) = 182.270304895951 x − 0.456765629587437
80 R² = 0.999778242678273

60

X
40

20

0
0 0.1 0.2 0.3 0.4

t(h) ln X A Dilution X
0 3.58849183 0.201 1;5 36.17947
1.25 3.64719016 0.213 1;5 38.36671
2.25 3.68449159 0.221 1;5 39.82487
15.5 4.16276694 0.355 1;10 64.24905
16.5 4.44297861 0.469 1;10 85.02783
17.5 4.70872059 0.611 1;10 110.91017
18.5 4.29789502 0.406 1;20 73.54482
19.5 4.57154215 0.533 1;20 96.69311
20.5 4.62297739 0.561 1;20 101.79667
21.5 4.60308501 0.55 1;20 99.7917
22.5 4.58836517 0.542 1;20 98.33354
23.5 4.50731698 0.5 1;20 90.6782
24.5 4.56207227 0.528 1;20 95.78176

t lag= 15h
μ= 0.27297683
tgen= 2.53921618
 X vs A

5951 x − 0.456765629587437
8273

0.3 0.4 0.5 0.6

ln X vs t
4.8

f(x) = 0.272976828358 x − 0.0659622856633044

4.6
R² = 0.999765909170335

4.4

4.2
ln X

3.8

3.6

3.4
0 5 10 15 20 25 30

t
358 x − 0.0659622856633044
70335

25 30
S μ 1/S 1/μ
0 0.014 #DIV/0! 71.4285714
1.1 0.042 0.90909091 23.8095238
19.8 0.398 0.05050505 2.51256281
24.6 0.431 0.04065041 2.32018561
50.1 0.586 0.01996008 1.70648464
99.3 0.684 0.01007049 1.4619883
138.9 0.644 0.00719942 1.55279503
152.6 0.686 0.00655308 1.45772595
191.8 0.652 0.00521376 1.53374233
246.3 0.696 0.00406009 1.43678161
294.7 0.726 0.00339328 1.37741047 R2=
322.2 0.796 0.00310366 1.25628141
348.1 0.686 0.00287274 1.45772595
360.7 0.699 0.00277239 1.43061516

μm= 0.76740081 h-1

Ks= 18.9954723 ppm
 1/u vs 1/S
25
f(x) = 24.7531308926079 x + 1.30313188473453
R² = 0.99986709515367
20

15

0.9998671 10

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
 1/u vs 1/S

+ 1.30313188473453

0.4 0.5 0.6 0.7 0.8 0.9 1

S (mol/L) μ(h-1) 1/S 1/μ
0 0 #DIV/0! #DIV/0!
3
0.00001 0.406 100000 2.46305419
0.00002 0.619 50000 1.61550889
0.00006 0.951 16666.666667 1.05152471 2.5
0.00008 1.02 12500 0.98039216 f(x) = 1.693
R² = 0.9999
0.0001 1.066 10000 0.9380863
2
0.00015 1.134 6666.6666667 0.88183422
0.0002 1.171 5000 0.85397096
0.0003 1.211 3333.3333333 0.82576383 1.5

1/μ
0.0004 1.232 2500 0.81168831
0.0008 1.265 1250 0.79051383
0.0016 1.282 625 0.7800312 1

0.005 1.294 200 0.77279753

0.5

μm(h-1) 1.3001165765
Ks(mol/L) 2.201862E-05 0
0
 3

2.5
f(x) = 1.69358836865901E-05 x + 0.769161795215672
R² = 0.999999627480353
2

1.5
1/μ

0.5

0
0 20000 40000 60000 80000 100000 1

1/S
00 100000 120000
 Sin=
μ(h )=D
-1 S(g/L) X(g/l) Yx/s= 0.0554185
0.3 4.5 3.26 0.04977099
0.25 4.1 3.36 0.05098634
0.2 1.6 3.96 0.05789474
0.12 0.8 4.06 0.05867052
0.08 0.4 4.16 0.05977011

Sin-S X(g/l)
65.5 3.26
65.9 3.36
68.4 3.96
69.2 4.06
69.6 4.16

X vs Sin-S
4.5
4
3.5 f(x) = 0.0556259866233272 x
R² = 0.994505806063655
3
2.5
X

2
1.5
1
0.5
0
65 65.5 66 66.5 67 67.5 68 68.5 69 69.5 70

Sin-S

Yx/s= 0.0556
 70 Xin= 0
g/g 1/S 1/μ
0.22222222 3.33333333
0.24390244 4
0.625 5
1.25 8.33333333
2.5 12.5

1/μ vs 1/S
14

12 f(x) = 3.97142645191248 x + 2.78809922599721

R² = 0.989092935913986
10

8
1/μ

0
0 0.5 1 1.5 2 2.5 3

1/S

9.5 70
 μmax= 0.35866729 h-1
Ks= 1.42442077 g/l

2.5 3
 assuming Xin=0
Flow rate(mL/h) S(μM) μ(h-1)=D 1/S 1/μ
10 17.4 0.01 0.05747126 100
15 25.1 0.015 0.03984064 66.6666667
20 39.8 0.02 0.02512563 50
25 46.8 0.025 0.02136752 40
30 69.4 0.03 0.01440922 33.3333333
35 80.1 0.035 0.01248439 28.5714286
50 100 0.05 0.01 20

μm(h-1) 0.13281704
Ks(μM) 210.118076
Dmax 0.13281704
Qmax 132.817044 mL/h
 120

100
f(x) = 1582.01138425282 x + 7.52915415644936
R² = 0.989175909143916
80

60
1/μ

40

20

0
0 0.01 0.02 0.03 0.04 0.05 0.06 0.07

1/S
0.06 0.07
t(h) S(g/L) X(g/L) 1/S av 1/S
1.5 19.91 0.58 0.05022602 0.05046798
4 19.72 0.74 0.05070994
6.5 19.47 0.95 0.05136107 0.05177657
9 19.16 1.22 0.05219207
11.5 18.77 1.56 0.05327651 0.05399055
14 18.28 1.98 0.0547046
16.5 17.67 2.51 0.0565931 0.05786485
19 16.91 3.16 0.05913661
21.5 15.97 3.96 0.06261741 0.06497874
24 14.85 4.92 0.06734007
26.5 13.53 6.06 0.07390983 0.07858689
29 12.01 7.36 0.08326395
31.5 10.33 8.81 0.09680542 0.10688224
34 8.55 10.33 0.11695906
36.5 6.77 11.86 0.14771049 0.17189446
39 5.1 13.29 0.19607843
μ=rx/X 1/μ
0.0969697 10.3125 1/μ vs 1/S
25
0.09953917 10.0462963
20 f(x) = 97.6585546759342 x + 5.22938344418713
0.09491525 10.5357143 R² = 0.999233998091645

15
0.09171076 10.9038462

1/μ
10
0.08648649 11.5625

0.07749627 12.9038462 5

0.06353187 15.7401316 0
0.04 0.06 0.08 0.1 0.12 0.14

0.04548708 21.9842657 1/S

μmax= 0.19122653 h-1

Ks= 18.6749914 g/L
μ vs 1/S

+ 5.22938344418713

0.1 0.12 0.14 0.16 0.18

1/S
Q S0 S D=µ 1/S
0.2 30 0.5 0.2 2
0.25 30 0.7 0.25 1.42857143
0.35 30 1.1 0.35 0.90909091
0.5 30 1.6 0.5 0.625
0.7 30 3.3 0.7 0.3030303
0.8 30 10 0.8 0.1
0.5 60 30 0.5 0.03333333
0.6 60 22 0.6 0.04545455
0.7 60 15 0.7 0.06666667

µ max = 1.118193 class 1,7

Ks = 2.32785419 class 4
Kis = 26.1780105 class 12,7

ALWAYS PLOT AND CHECK IF IT FITS THE MODEL

1/µ
5
1 tramo
4 6
2.85714286
2 5
f(x) = 2.08177625244477 x + 0.89425712806711
1.42857143 R² = 0.991521240534169
4
1.25
2 3
1.66666667
1.42857143 2

0
0 0.5 1 1.5 2 2.5

2 tramo
2.5

2
f(x) = 0.0381797689489997 x + 0.845731191885038
R² = 0.996702133434667
1.5

0.5

0
14 16 18 20 22 24 26 28 30 32
 2 2.5

28 30 32
S t 1/S 1/µ
16.3 0.22 0.06134969 0.22
0.45
3.2 0.3 0.3125 0.3
0.4
1.84 0.4 0.54347826 0.4
f(x) = 0.372548
0.35 R² = 0.9922385
0.3
a= K/µ = 0.1928 0.25
b= 0.3725
0.2
0.15
0.1

0.05

0
0 0.1

S t 1/S 1/µ
58.8 0.28 0.0170068 0.28
14.7 0.3 0.06802721 0.3 0.5
8.4 0.34 0.11904762 0.34 0.45
3.5 0.43 0.28571429 0.43 0.4 f(x) = 0.569301533
R² = 0.9948208249
0.35
0.3
0.25
0.2
a= K/µ = 0.2678
0.15
b= 0.5693
0.1
0.05
0
0 0.05

S t 1/S 1/µ
40.8 0.32 0.0245098 0.32 0.8
6.4 0.5 0.15625 0.5
0.7
2.3 0.69 0.43478261 0.69 f(x) = 0.867718821
0.6 R² = 0.9650324684

0.5

0.4

a= K/µ = 0.3253 0.3

b= 0.8677
0.2

0.1

0
0 0.05 0.1 0
 0.2

0.1

0
0 0.05 0.1 0

0.35
P a f(x) = 0.002853
0.3
22.5 0.1928 R² = 0.9993628
47.8 0.2678 0.25
69 0.3253
0.2

0.15
µmax= 7.72200772
k= -0.02239382 0.1

0.05

0
20 30

P b
22.5 0.3725
47.8 0.5693 1

69 0.8677 0.9
0.8 f(x) = 0.0105578
0.7 R² = 0.97178689

0.6
0.5
Ks= 0.87181467 0.4
Ks= 3.65517241
0.3
Ksavg= 2.26349354
0.2
0.1
0
20 30

P S tcalc t dif
22.5 16.3 0.22179389 0.22 1%
3.2 0.3325048 0.3 10%
1.84 0.43432357 0.4 8%
47.8 58.8 0.27844122 0.28 -1%
14.7 0.30940489 0.3 3%
8.4 0.34036856 0.34 0%
3.5 0.44151654 0.43 3%
69 40.8 0.34788548 0.32 8%
6.4 0.44616992 0.5 -12%
2.3 0.65396847 0.69 -6%
 1er tramo
0.45

0.4
f(x) = 0.372548470855127 x + 0.192750291139841
0.35 R² = 0.992238518839936
0.3
0.25

0.2
0.15
0.1

0.05

0
0 0.1 0.2 0.3 0.4 0.5 0.6

2º tramo
0.5
0.45
0.4 f(x) = 0.569301533219762 x + 0.267789608177172
R² = 0.994820824925061
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
0 0.05 0.1 0.15 0.2 0.25 0.3

3er caso
0.8

0.7
f(x) = 0.867718821886279 x + 0.325294087634249
0.6 R² = 0.965032468434976

0.5

0.4

0.3

0.2

0.1

0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
0.2

0.1

0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5

k and µmax
0.35

0.3 f(x) = 0.00285312967051892 x + 0.129486345632238

R² = 0.999362861752029
0.25

0.2

0.15

0.1

0.05

0
20 30 40 50 60 70 80

Ks
1
0.9
0.8 f(x) = 0.0105578852197873 x + 0.112928862961209
0.7 R² = 0.971786899111048

0.6
0.5
0.4
0.3
0.2
0.1
0
20 30 40 50 60 70 80

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0
0 10 20 30 40 50 60 70
0.4

0.3

0.2

0.1

0
0 10 20 30 40 50 60 70
60 70
60 70
Sf=50 P=22,5
S t 1/S 1/µ
16.3 0.22 0.06134969 0.22
3.2 0.3 0.3125 0.3
1.84 0.4 0.54347826 0.4

kobs 5.18805961
Ks obs 1.93280367

Sf=100 P=47,8
S t 1/S 1/µ
58.8 0.28 0.0170068 0.28
14.7 0.3 0.06802721 0.3
8.4 0.34 0.11904762 0.34
3.5 0.43 0.28571429 0.43

kobs 3.73427485
Ks obs 2.1259284

Sf=150 P=69
S t 1/S 1/µ
40.8 0.32 0.0245098 0.32
6.4 0.5 0.15625 0.5
2.3 0.69 0.43478261 0.69

kobs 3.07414133
Ks obs 2.6674903

kobs Ks obs P P*
5.18805961 1.93280367 22.5 119.8
3.73427485 2.1259284 47.8
3.07414133 2.6674903 69
 1/s vs 1/µ
0.8

0.7
f(x) = 0.867718821886279 x + 0.325294087634249
R² = 0.965032468434976
0.6

0.5

0.4 f(x) = 0.569301533219762 x + 0.267789608177172

1/µ

f(x) = 0.372548470855127 x + 0.192750291139841

R² = 0.994820824925061
0.3 R² = 0.992238518839936

0.2

0.1

0
0 0.1 0.2 0.3 0.4 0.5 0.6

1/S

kobs

f(x) = 0.79877141561744 x + 1.78169718270

R² = 0.964568679616887

ln(1-P/P*) ln kobs ln Ks obs

-0.2080247 1.64635976 0.65897163
-0.50915757 1.31755365 0.7542086
-0.85792733 1.12302562 0.98113807

-0.9 -0.8 -0.7 -0.6 -0.5 -

 R2 R2
0.96456868 0.96452868
-0.9 -0.8 -0.7 -0.6 -0.5 -

O.F= R2(1)*R2(2)= 0.93035416

µmax= 5.93992901
Ks= 1.70865782
 S µ 1/t
16.3 4.50525026 4.54545455
3.2 3.15813591 3.33333333
1.84 2.47698134 2.5
58.8 3.57142857
14.7 3.33333333
8.4 2.94117647
3.5 2.3255814
40.8 3.125
6.4 2
2.3 1.44927536

0.5 0.6

kobs K s obs
1.8
1.6
877141561744 x + 1.78169718270669
568679616887 1.4
f(x) = − 0.499770819324699 x + 0.535708
1.2 R² = 0.9645286802327

1
0.8
0.6
0.4
0.2
0
-0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 -0.9 -0.8 -0.7 -0.6 -0.5
 1
0.8
0.6
0.4
0.2
0
-0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 -0.9 -0.8 -0.7 -0.6 -0.5
 K s obs
1.2

1
0.499770819324699 x + 0.535708161404287
645286802327 0.8

0.6

0.4

0.2

0
-0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1
 0.6

0.4

0.2

0
-0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1
tf Cx Xcalc X-Xcalc squate
0- - -
15.25 0.36 0.1804448540013 0.17955515 0.03224005
17 0.24 0.1915286849659 0.04847132 0.00234947
22 0.32 0.2270927809211 0.09290722 0.00863175
29.67 0.3 0.2948984869781 0.00510151 2.60254E-05
31.25 0.26 0.3112052442861 -0.05120524 0.00262198
34.5 0.3 0.3476378722101 -0.04763787 0.00226937
37 0.36 0.3785400489624 -0.01854005 0.00034373
38 0.39 0.3916568569011 -0.00165686 2.74517E-06
40.75 0.32 0.430119329679 -0.11011933 0.01212627
42.5 0.38 0.4565394233478 -0.07653942 0.00585828
44.75 0.42 0.4929065186852 -0.07290652 0.00531536
50.75 0.52 0.6046832851711 -0.08468329 0.00717126
51.17 0.6 0.6133966531483 -0.01339665 0.00017947
56.75 0.8 0.7418077495517 0.05819225 0.00338634
52.75 0.76 0.6473151396719 0.11268486 0.01269788
64 1 0.9496140762925 0.05038592 0.00253874
66 1.1 1.0165645115456 0.08343549 0.00696148
68 1.06 1.0882351388139 -0.02823514 0.00079722
70 1.16 1.1649587447715 -0.00495874 2.45891E-05
72 1.2 1.2470915784788 -0.04709158 0.00221762
sum of squares = 0.10775962
 tf vs Cx
1.4

1.2

0.8

0.6

0.4

0.2

0
0 10 20 30 40 50 60 70 80

X0= 0.10733405
B= 0
K= 0.03406421
60 70 80
S0 S µ
200 168.7 0.255 0.3
132.9 0.152
82.3 0.0677 0.25
61.8 0.0409
51.5 0.0296 0.2
37 0.0163
650 640.2 0.256
0.15
530.2 0.176
295.1 0.0673
0.1
215.7 0.0374
139.7 0.0174
85.9 0.00722 0.05

1000 1063.7 0.249

760.3 0.133 0
20 40 60 80
473.2 0.065
297.4 0.0311
0.3
231.2 0.018
173.5 0.011
0.25
1400 1420.3 0.22
843.4 0.0939
0.2
287.1 0.0139
247.2 0.0106
210.9 0.00797 0.15

177.7 0.0058
0.1

0.05

0
0 200 400

1
S0 1/S^1,82 1/µ
70
200 8.844037E-05 3.92156863
0.0001365163 6.57894737 60
f(x) = 43580.8621623401 x + 0
0.000326567 14.7710487 R² = 0.999917072010267
50
0.0005500485 24.4498778
0.0007664977 33.7837838 40
0.0013991777 61.3496933
30
650 7.807383E-06 3.90625
1.100322E-05 5.68181818 20
3.196363E-05 14.858841
5.654481E-05 26.7379679 10

0
0 0.0002 0.0004 0.0006
 30

20

10

0.0001246644 57.4712644 0
0.0003020873 138.504155 0 0.0002 0.0004 0.0006

1000 3.098768E-06 4.01606426

5.709621E-06 7.51879699
2º
1.353376E-05 15.3846154
3.151515E-05 32.1543408 160

4.983588E-05 55.5555556 140

8.403785E-05 90.9090909 f(x) = 456871.967122358 x + 0.52
120 R² = 0.999979747276534
1400 1.830915E-06 4.54545455
4.727362E-06 10.6496273 100

3.360312E-05 71.942446 80
4.412165E-05 94.3396226
60
5.890886E-05 125.470514
8.045793E-05 172.413793 40

20

0
0 0.00005 0.0001 0.000

µmax 2.43368216
µmax 1.89969605
µmax 1.5595758
µmax 2.14730513 class 7,35

µmaxavg 2.0100647843

LN(S0) b ln(b)
5.2983173665 43580.8622 10.6823734 16
6.4769723629 456871.967 13.0321585 14 f(x) = 1.9964
6.907755279 1074362.58 13.8872381 R² = 0.99998
12
7.2442275156 2130864.52 14.5720383
10
p= 1.9965 class 2,06 8
Ks= 1.0286103391 class 1,6
6

4
ALWAYS CHECK THE VALIDITY OF THE MODEL 2

0
5 5.5
 0.3

0.25

0.2

0.15

0.1

0.05

0
40 60 80 100 120 140 160 180 0 100 200 30

0.25

0.2

0.15

0.1

0.05

0
200 400 600 800 1000 1200 0 200 400 60

1er tramo
100
90
f(x) = 43580.8621623401 x + 0.410909391797148 f(x) = 1074362.58055738
R² = 0.999917072010267 80 R² = 0.998588045630671
70
60
50
40
30
20
10

0.0002 0.0004 0.0006 0.0008 0.001 0.0012 0.0014 0.0016 0

0 0.00001 0.00002 0.0000
 40
30
20
10

0.0002 0.0004 0.0006 0.0008 0.001 0.0012 0.0014 0.0016 0

0 0.00001 0.00002 0.0000

2º tramo

200
x) = 456871.967122358 x + 0.526390974848631 180
² = 0.999979747276534
160 f(x) = 2130864.5200366
R² = 0.99997164330844
140
120
100
80
60
40
20
0.00005 0.0001 0.00015 0.0002 0.00025 0.0003 0.00035
0
0 0.00001 0.00002 0.00

calculation p and Ks
16

14 f(x) = 1.99649870624929 x + 0.102510560191465

R² = 0.999989477456207
12

10

0
5 5.5 6 6.5 7 7.5
 100 200 300 400 500 600 700

25

.2

15

.1

05

0
0 200 400 600 800 1000 1200 1400 1600

3ertramo
100
90
f(x) = 1074362.58055738 x + 0.641209312702713
80 R² = 0.998588045630671
70
60
50
40
30
20
10
0
0 0.00001 0.00002 0.00003 0.00004 0.00005 0.00006 0.00007 0.00008 0.00009
40
30
20
10
0
0 0.00001 0.00002 0.00003 0.00004 0.00005 0.00006 0.00007 0.00008 0.00009

4ºtramo
200
180
160 f(x) = 2130864.52003664 x + 0.465655831114773
R² = 0.999971643308445
140
120
100
80
60
40
20
0
0 0.00001 0.00002 0.00003 0.00004 0.00005 0.00006 0.00007 0.00008 0.00009