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Blood Smear Lab 2

The document outlines the procedure for preparing a blood smear, which involves spreading a thin layer of blood on a microscope slide and staining it for examination of blood cells. The aim is to investigate hematological issues, identify parasites, and assess the condition of blood cells. It includes details on materials needed, steps for preparation and staining, and concludes with an assignment for students to summarize their lab activities.

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32 views14 pages

Blood Smear Lab 2

The document outlines the procedure for preparing a blood smear, which involves spreading a thin layer of blood on a microscope slide and staining it for examination of blood cells. The aim is to investigate hematological issues, identify parasites, and assess the condition of blood cells. It includes details on materials needed, steps for preparation and staining, and concludes with an assignment for students to summarize their lab activities.

Uploaded by

saifgoran037
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Noble Institute –MLTDept.

Practical Human Physiology & Anatomy


First stage

Blood smear
Dr . Rabar Mohammed Hussein
PhD in Biological Science
[email protected]

Lab 2(Practical)
1
Previous week

Microscopic techniques

2
Outline
What is Blood smear
Aim of blood smear
Materials

3
Objective

How to prepare blood smear

4
BLOOD SMEAR AND
STAINING (BLOOD FILM)

A blood film or peripheral blood smear is a thin layer of blood


spreaded on microscope slide and then stained to allow
various blood cells to be examined microscopically.
AIM OF BLOOD SMEAR

- Examined to investigate hematological problems (disorder of


the blood).
-To look for parasite within blood such as malaria and filaria.
-To look number and shape of blood cells, and any change in
the appearance of blood cells.
-Provide report rapidly and at low cost, useful information
about the patient’s condition.
Three basic steps for preparation of blood film:
1- Preparation of blood smear 2- Fixation of blood smear 3-
Staining of blood smear
MATERIAL AND REAGENTS

Compound microscope.
Glass slides(Spreader and stationary)
Cotton.
Sterile finger pricking lancet.
Detol or alcohol
Leishman´s stain.
Buffer or D.W.
Cedar wood oil.
PREPARATIONS OF BLOOD
SMEAR AND STAINING :

Fill a capillary tube three-quarter full with the anticoagulated


specimen.
A drop of blood in the center of a clean glass slide 1 to 2 cm
from one end.
Place another slide (spreader) with smooth edge at an angle of
30 to 45° near the drop of blood.
Move the spreader backward so that it makes contact with drop
of blood.
Then move the spreader forward rapidly over the slide.
A thin or thick peripheral blood smear is prepared.
Dry it and then stain it
STAINING OF BLOOD
SMEAR

Blood cells have different structures, which take different


stains. Some are basophilic; others are acidophilic while some
cells accept neutral stain. Therefore, the stain used is
combination of these three. Such stains are called as
Romanowsky stain.
The three different types of Romanowsky stains are commonly
in use.
Leishman stain Giemsa’s stain Wright’s stain.
Each of the stain contains acidic stain, basic stain and buffer
solution. Generally, methylene blue are basic stains, and Eosin
are the acidic stains in use.
PROCEDURE:
Put the smeared slide on the rack.
Put 10 drop of Leishman stain on the blood smear & leave for 1
– 2 min.
Add double drop amount (20 drop) of buffered water or D.W.
leave it for 8 – 10 min.
Wash the slide with D.W.
Clean the back of the slide by filter paper.
Leave the slide to dry in air.
Exam the slide under microscope using oil immersion.
To obtain best smear stain Leishman stain used , because
there is no water in its content
ASSIGNMENT
Write a paragraph what we did in lab this week?
NEXT WEEK
Blood pressure
Thanks

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