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Biotechnology Principles - Processes

The document outlines the principles and processes of biotechnology, focusing on core techniques and the steps involved in genetically modifying organisms. It details tools used in recombinant DNA technology, such as restriction enzymes and cloning vectors, as well as the processes of isolating genetic material, DNA cutting, and gene amplification. Additionally, it discusses the role of bioreactors and downstream processing in biotechnology.

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akhil01exam
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20 views11 pages

Biotechnology Principles - Processes

The document outlines the principles and processes of biotechnology, focusing on core techniques and the steps involved in genetically modifying organisms. It details tools used in recombinant DNA technology, such as restriction enzymes and cloning vectors, as well as the processes of isolating genetic material, DNA cutting, and gene amplification. Additionally, it discusses the role of bioreactors and downstream processing in biotechnology.

Uploaded by

akhil01exam
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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BIOTECHNOLOGY:
PRINCIPLES &
PROCESSES
By: Dr. Anand Mani
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Biotechnology deals with:

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PRINCIPLES OF BIOTECHNOLOGY

1. Core techniques of modern biotechnology

2. Basic steps in genetically modifying an organism

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TOOLS OF RECOMBINANT DNA TECHNOLOGY


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1. Restriction Enzymes (‘molecular scissors’)
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Naming of the restriction enzymes:
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Types of Restriction enzymes:

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Separation and isolation of DNA fragments


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2. Cloning Vector

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Features required for cloning into a vector

a) Origin of replication (ori) b. Selectable marker (marker gene)

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c. Cloning sites

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d. Vectors for cloning genes in plants & animals

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3. Competent Host (For Transformation with
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Recombinant DNA)

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Other methods to introduce alien DNA into host cells

PROCESSES OF RECOMBINANT DNA TECHNOLOGY

1. Isolation of the Genetic Material (DNA)

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2. Cutting of DNA at Specific Locations

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3. Amplification of Gene of Interest using PCR

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4. Insertion of Recombinant DNA into HostCell

5. Obtaining the Foreign Gene Product

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Bioreactors The bioreactor has

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6. Downstream Processing

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