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Mod1 - Design Your Experiment

The document outlines an experiment for BIOL 1106 to investigate the relationship between molecular size, solute permeability, and water movement in plant cells using various solutes. Students are required to determine the isosmotic concentration, collect quantitative data, and present results graphically, with specific guidelines on replicates and concentrations. It also includes mathematical formulas for preparing solutions and dilutions, as well as sections for students to describe their experimental design and hypotheses.

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Walid Elmakdah
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14 views3 pages

Mod1 - Design Your Experiment

The document outlines an experiment for BIOL 1106 to investigate the relationship between molecular size, solute permeability, and water movement in plant cells using various solutes. Students are required to determine the isosmotic concentration, collect quantitative data, and present results graphically, with specific guidelines on replicates and concentrations. It also includes mathematical formulas for preparing solutions and dilutions, as well as sections for students to describe their experimental design and hypotheses.

Uploaded by

Walid Elmakdah
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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BIOL 1106 Module 1

Design Your Experiment


You will design an experiment to explore the relationship between how molecular size and
solute permeability affect the movement of water into and out of plant cells.
 You may choose sugars (glucose or sucrose) or salts (NaCl, KCl, CaCl2).
o 1 M NaCl, 1 M KCl, 1 M CaCl2, 1 M glucose, 1 M sucrose
 You will determine the concentration at which a solute and the cellular
concentration of solutes in the cell are equal. This is the isosmotic concentration
and it is the point in which 50% of the cells are plasmolyzed.
 You must collect quantitative data and present it in graphical form.
 You may work with either Elodea or red onion cells, or compare results for both
plants.
 You must do no less than 3 replicates for each condition in order to calculate
standard deviation.
 You must test a minimum of 5 different concentrations (dilutions) for each solute
selected in order to generate a graph.
 You can estimate the isosmotic point based on your graph, using the following
example:

% cells plasmolyzed

50% plasmolyzed

Solution 1
Solution 2
Solution 3

[Solution]

Some useful math:

We will provide the salt or sugar solutions at a concentration of 1 M. To provide you a


frame of reference, a 15% NaCl solution (from Day 1 of the lab module) is equivalent to 2.6 M.
The “M” stands for molarity and is defined as the number of moles of solute (sugar or salt in
this case) dissolved into 1 L of solvent (water in this case). Consult the “Preparing Solutions”
supplemental document posted on Blackboard.
BIOL 1106 Module 1

The units are mol/L. One mole is equivalent to 6.022 x 10 23 (Avogadro’s number)
molecules of a substance. Weighing out 1 gram of NaCl and 1 g of sucrose does not mean you
have the same number of molecules of each substance. You have the same mass of each, but
NaCl has a formula weight of 58.4 g/mol and sucrose has a formula weight of 342.3 g/mol. To
make a 1 M solution of NaCl would require 58.4 g NaCl dissolved in water with a final volume of
1L. To make a 1 M solution of sucrose would require 342.3 g/mol sucrose dissolved in water
with a final volume of 1L.

You will conduct your experiment using molarity (M). To dilute the 1 M concentration
stock solutions provided to your desired molarity, you will use the following formula:
C1V1 = C2V2
C1= concentration of your stock solution
C2=desired concentration of your final product
V1=volume needed of the stock solution
V2 =volume desired of the final product

Sample Problem:

Dr. Medrano wants to make 1 ml of a 0.2 M solution of NaCl. She has a 1 M NaCl stock solution
handy on the bench. How should she make the desired solution?

Step 1. Apply the C1V1 = C2V2 formula.


C1 = 1M NaCl
C2 = 0.2M NaCl
V1 = (x)
V2 = 1 ml (1000 l)

(1 M NaCl) (x) = (0.2M) (1 ml)


x = 0.2 ml

Our pipettors are in a µl scale, so we should convert this to what we will measure on the
instrument we will use.
(0.2 ml) (1000 µl/ml)= 200 µl.

Step 2. Add 200 µl of the 1 M NaCl solution to 800 µl of water to yield the final volume of 1 ml.

Depending on the solution of your choice, you may need to consult the concept of osmolarity
as well, to discuss your results on the Experimental Summary assignment.
BIOL 1106 Module 1

Design Your Own Experiment


BENCH: ______
Team Members: _____________________________________________________

Describe the problem, or, ask a question:

State the hypothesis:

State the null-hypothesis:

Describe your control:

Describe your experimental group


Independent variable(s):

Dependent variable(s):

Materials needed:

Write down the protocol steps:

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