Working in a

Laboratory
Maxime FLORENT 2019/2020
Summary
• Security
• Writing an experimental procedure
• Training
Security
• Stay safe
Being safe in a laboratory is the first step to work correctly.
Safe working protects: You / Other lab workers / Cleaners
Visitors / Your work

• Follow the rules

Given by the teachers or lab assistant

• Know your environment

Before starting you need to know where are
- The Emergency Exit
- The Extinguishers
- The first Aid kit
 Security
• Clothes

• No rings
• No open shoes
• Only pants that cover all your legs
• No tights
• If you have long hair you must attached them !
Security
• Gloves
The gloves are really useful in a Laboratory but can also make things worst
There are many different types of protective gloves : Nitriles/ Latex / Vinyl / PVC
In your PW at UFAZ you will only use nitrile gloves
• When should I used gloves ?
Security
• Gloves
The gloves are really useful in a Laboratory but can also make things worst
There are many different types of protective gloves : Nitriles/ Latex / Vinyl / PVC
In your PW at UFAZ you will only use nitrile gloves
• When should I used gloves ?
Only when I am manipulating something dangerous !
(Acids/Bases/CMR reagents..)
I put my gloves on just before my manipulation, and I removed them right after !
• Why ?
Security
• Gloves
The gloves are really useful in a Laboratory but can also make things worst
There are many different types of protective gloves : Nitriles/ Latex / Vinyl / PVC
In your PW at UFAZ you will only use nitrile gloves
• When should I used gloves ?
Only when I am manipulating something dangerous !
(Acids/Bases/CMR reagents..)
I put my gloves on just before my manipulation, and I removed them right after !
• Why ?
If my gloves are dirty (by something visible or not), When I touch something else,
It will be contaminating too.
(Wardrobe, Pen, Notebook, My clothes, My face, My friends arms etc…)
 Security
• Fumehood
You will, most of the time, work under a
fume hood, the goal is to vacuum the vapor
To avoid breathing them

• A Fume Hood is useful only if

It is on …
The sash is closed at list at the middle
The sash cover your face

Every manipulation must be achieved under the Fume Hood

You need to keep your work place clean and tidy as much as possible
 Security
• Fumehood
You need to keep your work place clean and tidy as much as possible
 Security
• Glassware
• Before using a glassware you need to :
• Be sure of the cleanliness, for the success of your experiment and also to avoid any
unwanted reaction
• Check glassware for stars or cracks.
• Dispose of chipped or broken glassware
it is a risk to you and others
• You will find in every laboratory a broken glassware disposal

• After using a glassware you need to :

• Clean it, an unclean glassware can easily be washed right after the utilization
with the appropriate solvent, But after a few days it is really more difficult
 Security
• Reagents
• The reagents are the biggest source of insecurity in a Laboratory
• Read and reread labels carefully to make sure that you are using the right chemical.
• To avoid as much as possible any risk you must know the hazards of the materials being used.
• Check the pictograms
• Use data sheets and other relevant information (eg EH40) to assess risk
• Consider not only reactants but also intermediates, products and waste
• Determine safe working procedures
• Consider correct waste disposal

• Each reagent and solvent has its own code called CAS number.
 Security
• Pictograms
 Security
• SDS (Safety Data Sheet)
An SDS (formerly known as MSDS) includes information such as
• the properties of each chemical;
• the physical, health, and environmental health hazards;
• protective measures;
• safety precautions for handling, storing, and transporting the
chemical.
It provides guidance for each specific chemical on things such as:
• Personal Protective Equipment (PPE)
• First aid procedures
• Spill clean-up procedures
https://chemicalsafety.com/sds1/sdsviewer.php?id=30035914&name=Chloroform
 Security
• Waste disposal
Waste disposal is a normal part of any science laboratory. As teachers or students
perform laboratory experiments, chemical waste is generated. These wastes should be
collected in appropriate containers.

Many laboratory explosions have occurred from inappropriate mixing of wastes, such as
mixing acid waste with organic wastes, so be sure that waste materials are compatible. Mixing
acid with any organic materials may result in an over pressurization of the waste container and
release of the chemical into the workspace.
 Security
• Waste disposal
How can I know where I should put each waste?
 Security
• Waste disposal
How can I know where I should put each waste?
 Security
• Waste disposal
How can I know where I should put each waste?
 Security
• Waste disposal
How can I know where I should put each waste?
 Security
• In case of Fire
• In each Laboratory you should have at your disposition :
 Security
• In case of Fire
• In each Laboratory you should have at your disposition :
• Extinguisher be careful each extinguisher as is own utility.
• Fire Blanket can be used to extinguish a small fire or to wrap around a
person in case of a fire.
 Security
• In case of projection
• If during a practical work you have a product on your skin and you start to
feel burns or itching
• Put the burned area under water for at least 5 to 10 minutes
• If you have a projection in your eyes
• Use the eyes washer immediately
 Security
• In case of projection
• If during a practical work you have a product on your skin and you start to
feel burns or itching
• Put the burned area under water for at least 5 to 10 minutes
• If you have a projection in your eyes
• Use the eyes washer immediately
• If you have toxic product on yourself ( lab coat or clothes)
• Removed them and use the security shower if needed
 Security
• In case of projection
• If during a practical work you have a product on your skin and you start to
feel burns or itching
• Put the burned area under water for at least 5 to 10 minutes
• If you have a projection in your eyes
• Use the eyes washer immediately
• If you have toxic product on yourself ( lab coat or clothes)
• Removed them and use the security shower if needed

There is also a First Aid kit in each laboratory, in case of cut or burn