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The document outlines a procedure for separating colored components from a mixture of red and blue inks using ascending paper chromatography. It details the required apparatus, materials, and step-by-step instructions for conducting the experiment, including how to measure and calculate Rf values. Precautions are also provided to ensure accurate results during the chromatography process.

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Ayush Gupta
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8 views2 pages

Selfstudys Com File

The document outlines a procedure for separating colored components from a mixture of red and blue inks using ascending paper chromatography. It details the required apparatus, materials, and step-by-step instructions for conducting the experiment, including how to measure and calculate Rf values. Precautions are also provided to ensure accurate results during the chromatography process.

Uploaded by

Ayush Gupta
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Separate the Coloured Components Present In the Given

Grass/Flower By Ascending Paper Chromatography &


Determine Their Rf Values

Apparatus
Gas jar, glass rod, filter paper strip (What man No. 1 filter paper), jar cover, fine capillary
tube.

Requirement
A mixture of red and blue inks, alcohol and distilled water.

Procedure

1. Take a What man filter paper strip (20 x 2 cm) and draw a line with pencil above 3
cm from one end. Draw another line lengthwise from the centre of the paper as
shown in Fig.

2. With the help of fine capillary tube, put a drop of the mixture of red and blue inks
at the point P. Let it dry in air. Put another drop on the same spot and dry again.
Repeat 2-3 times, so that the spot is rich in mixture.
3. Suspend the filter paper vertically in a gas jar containing the solvent (eluent) with
the help of a glass rod in such a way that the pencil line (and the spot) remains
about 2 cm .above the solvent level (50% alcohol + distilled water).
4. Cover the jar and keep it undisturbed. Notice the rising solvent along with the
mixture of red and blue inks. After the solvent has risen about 15 cm you will
notice two different spots of blue and red colours on the filter paper.
5. Take the filter paper out of the jar and mark the distance that the solvent has risen
on the paper with a pencil. This is called the solvent front.
6. Dry the paper. Put pencil marks in the centre of the blue and red spots.
7. Measure the distance of the two spots from the original line and the distance of
the solvent from the original line.
8. Calculate the Rf values of the blue and red inks by using the formula :

Observations and Calculations

Note: In the above experiment, crush fresh flowers or grass in a mortar and extract the
juice with acetone. Use this juice for making the spot.

Precautions

1. Use good quality pencil for drawing the reference line so that the mark does not
dissolve in the solvent in which the chromatography is carried out.
2. Always make use of a fine capillary tube.
3. Keep the jar undisturbed and covered during the experiment.
4. A spot should be small and rich in mixture.
5. Allow the spot to dry before putting the strip in the jar.
6. Keep the strip erect. Do not let it to be curled.
7. Do not allow the spot to dip in the solvent.

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