Subject Index

Abrus precatorius 250 Amplified random intergeneric

Acacia catechu 250 space analysis (ARISA) 4
Acacia mangium 389 Amplified r DNA restriction
Acacia nilotica 250 analysis (ARDRA) 4
Acetosyringone (AS) 22 Analysis program 48
Acid fuchsin (AF) 383 Anamorphic fungi 1
Acid phosphatase 214, 239 Anastomosis 253
ACPase 214 Aneura pinguis 250, 308
Acquired immune system 94 Annealing 125, 244
Actinomycetes 170, 369 Antibody 73, 89
Adaptive immune system 94 Antigenic determinant 77
Adenine hemisulfate 152 Antigen-presenting cell 94
Adhatoda vasica 250 Antioxidant capacity 350
Adjuvant 75 Antisense orientation 137
Adjuvant alum 75 Antisense RNA technology 137
Adjuvant FCA 75 Arabidopsis 214, 356
Aeroponic 386 Arabidopsis beta-amylase(BMY8)
Agaricus bisporus 22 gene 139
Agarose gel 170 Arabidopsis thaliana 112, 249
Agrobacterium rhizogenes 389 Aracon tube 310
Agrobacterium tumefaciens 22 Araucariaceae 377
Alkaline phosphatase 215 Arbuscular mycorrhiza (AM) 183,
Allium cepa 389 247, 397
Allogeneic 101 Arbuscular mycorrhiza fungi
Allogeneic recipient 102 (AMF) 319, 333
Amanita 239 Argonaute 135
Aminopterin 82 Artemisia annua 250
Ammonia oxidizers 191 Arthrobotrys 11
Ammonium bicarbonate 162 Arthrobotrys oligospora 8
Ammonium persulfate solution 88 Ascitic fluid 80
amoA 301 Ascomycetes 1
Amplicons 296 Ascomycota 345
Amplification 189, 244 Ascorbate 351
Amplification plot 61, 71 Aspergillus 254
416 Subject Index

Aspergillus awamori 22 Blast search 57

Aspergillus medium 239 Blocking buffer 226
Auriculariales 248 Blumeria graminis 348
Auto-fluorescence 384 Boletus 239
Automated chromatogram 38 Botrytis cinerea 22
Automated fluorescent dye 37 Bradford dye 157
Automated sequencing 37 Bradford method 157
Avena sativa 386 Brassica oleracea
Axenic culture 386 var. Capitata 249
Azadirachta indica 250 BRET 146
Bromophenol blue 241
Bacillus 191, 369 Broth 254
Bacopa monniera 250 Burkholderia 290
Bacterial artificial chromosomes
(BACs) 371 Cadmium 322, 337
Barley chemical induced Caenorhabditis elegans 141
protein 1 351 Calcofluor 368
(BAS) 385 Capillary electrophresis 285
Baseline 62 Capillary sequencers 38
Basidiomycetes 1 − ABI prism 310
Basidiomycota 249, 308 genetic analyzer 38
BD-cloning vector 26 − ABI prism 3700
Bicarbonate buffer 163 DNA analyzer 38
Bifacial leaf 271 Carcinogen 114
Big Dye terminator reactions 40 Cassia angustifolia 250
Biochemical affinity blotting 146 Catalase 345
Biochemical CCD Camera 38
co-immunoprecipitation 146 Cd 334
Biochemical cross-linking 146 Cd2+ hyperaccumulator 357
Biochemical far-western 146 cDNA 53
Biochemical polyacrylamide gel 146 cDNA arrays 53
Biochemical protein affinity cDNA library 149
chromatography 146 Cell lysis 156
Biogenesis related protein 20 Cell-mediated lympholysis
Bioinoculants 372 (CML) 99
Biological control agent 254 Cell proliferation 148
Biological hardening 254 Cell suspension 189
Bioluminescence marker gene 370 Cellulose 201
Biomass determination 198 Central lymphoid tissue 94
Biophysical parameters 328 Chain termination
Biophysical phenomics 319, 338 dideoxynucleotide 36
Biophysical phenotype 319, 328 Chalcone synthase 133
Biophysical phenotyping 320 Chemical biology 282
Bioprotector 308 Chemical mutagenesis 140
Biotin-UTP 119 Chemotherapy 53
BLAST analysis 44 Chimeric PCR products 8
Subject Index 417

Chl a fluorescence 320, 322 Culture-independent -FAME 167

Chl a fluorescence transient 325 Culture-independent method 167
Chl a fluorescence transient Culture-independent -RISA 167
O-J-IP 327 Culture-independent-Soil DNA 167
Chlamydospore 253 Cy3 126
Class I MHC molecule 99 Cy5 126
Class II MHC molecule 97 Cyclophilin 20
Chlorazol black E 383 Cyclotron Resonance Fourier 281
Chlorophytum borivillianum 250 Cymbopogon martinii 250
Chromatin modification 135 Cytokines 96
Chromatin remodeling 141 Cytometric bead array (CBA) 98
Chromium 99 Cytoplasmic protein 148
Chromosomal aberration 356 Cytotoxic activity 99
Ch. Tuberosum 250 Cytotoxic T lymphocyte (CTL) 99
Cicer arietinum 250
Cis-regulatory element 149 16Sr DNA clone library 295
Citrate buffer 185 Dactylaria 11
Clonal proliferation 96 Dactylorhiza fuchsia 249
Clone library 295 Dactylorhiza incarnata L. Soo’ 250
Cloning 80 Dactylorhiza maculata L. Verm. 250
Cluster 240 Dactylorhiza majalis Rchb. F. 250
Cochliobolus sativus 347 Dactylorhiza purpurella 249
Coffea arabica 250 Daucus carota 250
Co-immunoprecipitation 146 DDNTP 36
Colonization 214 DEAE-Sephadex 217
Colorimetric assay 147 Dehydroascorbate (DHA) 351
Column chromatography 217 Dehydroascorbate reductase
Community composition 182 (DHAR) 351
Comparative CT method 59 Dehydrogenase 239
Competent Yeast Cells 26 Delbergia sisso 250
Complimentary sequence 115 2D-Electrophoresis 161
Computational methods 145 Denaturation 125
Constitutive expression 138 Denaturation cycle 244
Coomassie blue staining 86 Denitrification 301
Coomassie brilliant blue 156 Densitometric scanning 121
Corynebacterium glutamicum 358 DEPC 114
Cosmid 371 Desoxyribonucleotide triphosphate
Co-suppression 133 (DNTP) 297
Craterocolla 214, 248 Desulfovibrio 194
Craterocolla cerasi 248 Detergent 117
Cruciferaceae 249 Dexamethasone 138
Cryostat section 117 Dextran sulfate 118
CTAB 242 (DGGE) 7, 189, 295
CT value 60 DGGE Digitization 195
Cultivar Annabell 347 DGGE GC tail 192
Culture-independent -DGGE 167 DGGE Gel-191
418 Subject Index

DGGE Gradient 194 Efibulobasidium rolleyi 248

DGGE Normalization 195 EGFP Primers 30
DGGE Separation 192 Electrochromatography 285
DGGE Sequencing 193 Electropherograms 45
Dicer 134 Electrophoresis 86
Dichroic mirror 276 ELISA 88, 225
Diethylpyrocarbonate 120 ELISA – Competitive 88
Digoxigenin 119 ELISA – Indirect ELISA 88
Dilution buffer 226 ELISA Sandwich 88
Dilution plating technique 2 EMS 310
DIS 147 EMS mutant lines 309
Disinfestation 387 Emulsification 223
Dissection 273 Endogenous control 57
Dissecting microscope 273 Endogenous genes 141
Dithiothreitol (DTT) 118 Endonucleases 135
Diversity 2, 188 Endonucleolytic cleavage 136
DNA 115, 189, 297 Endophyte 345
DNA amplification 244 Energy cascade 320
DNA-BD fusion vector 26 Energy flux 330
DNA binding domain 147 Enzyme activity 216
DNA binding proteins 149 Enzyme slippage 45
DNA-CTAB complex 242 Enzyme slippage
DNA extraction 189 homopolymer region 45
DNA fingerprint 238 Eosinophils 98
DNA methylation 141 Epitope 73
DNA repair gene radA 371 Equilibration buffer 160
DNA sequences 147 Ergosterol 182, 189
DNA sequencing 35 Ericoid mycorrhizas 239
DNA Silver staining 175 EST database 126
DNA size marker 298 Esterase 239
DNA staining 175 Estradiol 139
DNA staining Ethanol 138
Ethidium bromide 175 Ethanol precipitation 42, 49
DNA Sybr gold 175 Ethidium bromide 244
Dot/slot blot hybridization 112 Ethyl-methanesulfonate 310
Double interaction screen 149 ETS 5
dsRNA 135 Excess dntps 48
Duplicated gene 237 Extracellular pathogens 96
Dye-terminator 46 Extrachromosomal 235
Extra-radical mycelium (ERM) 384
Ecological niches 1
Ectomycorrhiza 271 FACS 101
Ectomycorrhizal fungi 238 6-FAM 297
Effector cell 101 FAME 165, 296, 381
Effector immune response 93 FAME extraction 183
Efibulobasidium 214 Fast fluorescence kinetics O-J-IP
Subject Index 419

− Fast fluorescence rise O-J-I-P 322 Fumigation 202

Fatty acid 182 Fumigation extraction method 205
Fatty acid methyl ester (FAME) 83, Functional blocks of PSII 323
298 Functional building block 320, 338
FCA 73 Functional genomic 134
Festuca rubra 355 Fusarium 2
FIA 73 Fusarium culmorum 348
Ficoll Hypaque 105 Fusarium oxysporum 22
Fine root 280 FWHM 286
Fingerprinting 8, 239
First-strand cdna 124 Gaeumannomyces graminis 251
FISH 4 GAL4AD fusion library 26
Flavonoids 281 GAL4 protein 149
Flourescein isothiocyanate 368 Gcfung primers 9
Flow cytometer 103 GC-rich template 40
Flow cytometry 96 gDNA contamination 58, 69
Fluorescein diacetate 205 gDNA dnase I digestion 58
Fluorescence 100, 323 Gel-based ABI prism
Fluorescence correlation 377 sequencer 38
spectroscopy 144 Gene expression 53
Fluorescence microscopy 385 Gene silencing 133
Fluorescence resonance energy Genetic material 111
transfer (FRET) 55 Genetic polymorphism 245
Fluorescence transient 325 Genetically engineered
Fluorescent antibody technique 369 microorganism (GEM) 372
Fluorescent dye 126, 297 Genomic DNA 298
Fluorescent FRET Gigaspora margarita 215, 381
hybridization probe 54 Glomalean fungi 377
Fluorescent molecular beacon 54 Glomeromycota 345
Fluorescent reporter 54 Glomus caledonium 320
Fluorescent Scorpion probe 54 Glomus coronatum 381
Fluorescent stains 368 Glomus etinicatum 407
Fluorescent Syber green 54 Glomus intraradice 215, 390
Fluorescent Taqman probe 54 Glomus mosseae 320, 389
Fly agaric 280 Glomus tenuis 379
Formamide 118 Glomus versiforme 390
Fosmid 371 Glucan-water dikinase 312
FPS 144 Glutaraldehyde 223
Fragment length polymorphism 297 Glutathione 351
Freeze-drying 274 Glycine max 250
FRET 144 Glycobiology 283
Freund’s complete 222 Glycoprotein 214
Freund’s incomplete 222 GPCR’S 18
Freund’s incomplete adjuvant GPCR – STE3 20
(FIA) 75 Graft versus host (GVH)
FTICR-MS 286 Reaction 102
420 Subject Index

Gram negative bacteria 182 Hydrophobicity 285

Gram positive bacteria 182, 189 Hydroponic 388
Hygromycin (hph) 29
Haemophilus influenzae 112 Hypersensitive reaction 344
Hairpin RNA 137 Hyphal mantle 271
Handy-PEA 325 Hypocreales 9
Handy-PEA fluorimeter 322 Hypoxanthine 78
Hartig net 271, 279
Haustorium 350 ICR-FT/MS 286
Hebeloma cylindrosporum 22 Image analysis 163
Helicobacter pylori 358 Immune cell 107
Helotiales 9 Immune response 93
Helper T cell 98 Immune system 93
Helper Th1 cell 98 Immunization 76
Helper Th2 cell 98 − Booster 76
HEPES buffer 82 Immunization Pre-immunized 76
Herbivore 377 Immunization Primary 76
Hexamer 68 Immunizing antigen 96
Hexanol extraction 205 Immuno-detection 227
HhaI 297 Immuno-fluorescence 228, 240
Hi-Di formamide 49 Immunogen 74
High template DNA 49 Immunoglobulin 73, 225
HinfI 297 Immunogold 240
Histidine 147 Impurities
Histochemistry 271 − Ethanol 43
Histocompatibility complex 97 − Phenol 43
Histogram 103 − Salt 43, 49
hma2 357 Induced pathogen resistance 347
hma3 357 Induced systemic resistance
H2O2 detection 349 (ISR) 344
Homologous recombination 137 Inducer 134
Homopolymer regions 49 Inducible RNAI 138
Hordeum vulgare 350 Inhibitor 157
HTP 285 Innate immune response 94
Human genome project 35 Innate memory immune
Humic acid 190 response 93, 96
Humic removal 190 Inserted membrane technique
Humic substance 189 (IMT) 385
Humoral immune response 96 In situ hybridization 53
Humoral immunity 95 Interaction library 26
Hybridization 112, 115 Internal transcribed spacers (ITS) 5
Hybridization probes 54 Intron 137
Hybridoma 77, 80 In vivo vitality analysis 319, 338
Hydrodynamic 142 Iodonitrotetrazolium formazan
Hydrologic cycle 201 (INTF) 208
Hydrophilicity 285 Ion exchange chromatography 217
Subject Index 421

Isoelectric focusing 156 Manual sequencing 36

Isoelectric point 156 Mass spectrometry 164, 287
MATH protein 311
Jasmonate induced protein 351 Maximum quantum yield
Jasmonic acid 281 of primary photochemistry 335
JIP-test 328, 335 Medicago sativa 386
Meloidogyne incognita 139
Keratin 156 Membrane filtration technique
Knop solution 260 (MFT) 385
mer R 301
L-arginine 152 Mercaptoethanol 86
Laccaria bicolor 238 Mercury resistance 301
Lactarius 239 Metabolomic 281, 286
Lactophenol 382 Metagenomic library 295
Landsberg errecta 312 Metagenomics 371
Lbras gene 20 Methane oxidation 301
Leucine 147 Methanosarcina acetivorans 358
LHC II 321 MHC molecule 96
LHC I 321 Microarray 126, 317
Lignin 201 Microarray analysis 112
Lipid extraction 186 Microbe interaction 307
Lipidomics 283 Microbial activity 207
L-lysine 152 Microbial biomass 201, 206
L-methionine 152 Microbial communities 182
Loliom perenne 355 Microbial dehydrogenase 202
L-tyrosine 152 Microbial enzyme 201, 207
L-uracil 152 Microbial fluorescein diacetate 207
Lux gene 370 Microbial fumigant 206
Lycopersicum esculentum 389 Microbial hexanol 206
Lymphocytes 93 Microbial hydrolysed 207
Lymphoid organs 94 Microbial soil respiration 201
Lymphoid tissue 94 Micromass MALDI 164
Micromass Q-TOF 164
Macrophages 79 Micromass Q-TOF
Macrostate 322, 338 ultrahigh resolution
MACS – negative selection 104 mass spectrometry 283, 292
MACS – positive selection 104 Microphotometry 274
Magnaporthe grisea 22 Micropropagated plantlet 247
Magnetic activated cell sorting MicroRNA 136
(MACS) 105 Microsatellite 238
Magnolia liliiferai 9 Microstate 321, 338
MALDI 164 miRNA 135
MALDI TOF 164 Mitomycin C 101
MALDI-TOF MS 164 Mixed-lymphocyte reaction
Manual-chain termination (MLR) 101
dideoxynucleotide 36 Mixed templates 45
422 Subject Index

MLR 101 Northern blot analysis 112

MMN agar medium 259 No-RT (without RT enzyme)
Molecular breeding 142 control 68
Molecular technique 4 nosZ 301
Monacrosporium 11 NS1 9
Monoaxenic culture 389 NTC (no-template control) 68
Monoclonal antibody 77, 103 NTS 5
Most probable number (MPN) 379 Nuclear 248
MspI 297 Nuclear-Encoded Ribosomal DNA
Multicapillary Electrophoresis Gene 5
(MCE) 38 Nuclear magnetic resonance
Multiplex limiting Nuclear rDNA 248
primer concentration 70 Nutrient film technique 386
Multiplex PCR 59, 70 Nylon membrane 114
Multivariate analysis 188
Mycorrhiza 377 Obligate endosymbionts 332
Mycorrhizal colonization 214 O-J-I-P 329, 333
Mycorrhizal dependency 385 − Fluorescence transient 325
Mycorrhization 319, 338 Oligo dT 68
Mycorrhizosphere 385 Oligonucleotide 240
Myeloma and spleen cell 79 O-L-K-J-I-H-G-P 329
Myeloma (tumor) cell 78 − Fluorescence transient 328
− ORFG 4
N–1 primers 46 Omniscript reverse transcriptase 59
N-acylhomoserine lactone One-hybrid system 149
(AHL) 281, 290 − Motif 149
nah gene 370 Optimization 322
Native PAGE 218, 220 Optimize signal fluorescence 299
Neutral trehalase 276 Orchid mycorrhizae 249
Nicotiana attenuata 250 Oryza sativa 250
Nicotiana tabaccum 250 Osmium 230
nif genes 191
nifH 301 L-phenylalanine 152
nirS 301 PAGE 158
Nitrification 301 Palisade parenchyma 271
Nitrite reduction 301 Papain 73
Nitrocellulose membrane 221 Para-formaldehyde 228
Nitrogen fixation 301 Parenthosome 253
(NMR) 286, 290 Passive reference dye 61
NMR Spectroscopy 283 Pathogenesis related genes
Non targeted analysis 283 (PR genes) 344
Non targeted metabolomics 282, Pathogenesis related protein 1 351
286 Pathogenesis related protein 5 351
Normalized reporter (Rn) 61 Pathogen resistance 343
Northern analysis 28 Pathogens 94
Subject Index 423

Paxillus involutus 22 Piriformospora indica 214, 307, 337

pBGgHg 23 Pisum sativum 250
PCA 290 Plant – growth 343
PCR 4, 189 Plant growth residues 182
PCR-based technique 238 Plant microbe interaction 307
PCR – pitfall 192 Plasma cells 95
PCR – primer 191 Plasmid 371
PCR-RFLP 4 Plasmid isolation 43
Penicillium 2 Pleosporales 9
Peptidase 239 PLFA 181
Performance index 328, 337 PLS-DA 290
Peripheral lymphoid tissue 94 pmoA 301
Peritoneal cavity 80 Polyacrylamide gel 296
Peritoneal macrophages 79 PolyA tail 124
Peroxidase 239 Polyclonal antibodies 73
Persulfate 219 Polymer 38, 49
Petroselinum crispum 250 Polymerase 297
Petunia 133 Polymerase chain reaction
pGADT7-Rec 26 (PCR) 190
PGPR 364 Polymerase DNA 298
Phellesgate 139 Polymerase enzyme 189
Phoretix TM 163 Polymorphism 238
Phosphatase 214 Polyvinylidene difluoride 165
Phosphate 39 Ponceau S stain 221
Phosphate buffer saline 228 PopOff1 139
Phospholipid 182 Populus Esch5 252
Phospholipid extraction 185 Populus tremula 250
Phospholipid fatty acid 182 Positive PCR control 68
Phospholipid fractionation 187 Post-transcriptional 133
Phosphorimager 315 Potassium ferricyanide 162
Phosphorus mobilizer 254 Potato dextrose agar 260
Phosphorus transporter 254 Powdery mildew 350
Phosphorylation 20 Prehybridization 118
Photo-multiplicator (PMT) 128 Primer 7
Photosynthetic apparatus 319 Primer design 43, 56
Photosystem II 321 Primer dimer 49
Phylogenetic Diversity 10 Probe 114
Phylogenetic study 248 Probe design 56
Phylogeny 7 Prokaryote 237
Phylotype 9 Proliferation 96
Phytohormones 281 Propidium iodide 104
Phytopromotional 254 Prosopis chilensis 250
Phytopromotional effects 254 Prosopis juliflora 252
Picea abies 239 Proteinase K 117
Pinus resinosa 20 Protein A sepharose 84
424 Subject Index

Protein extraction 156 Relative variable fluorescence 326

Protein–protein interaction 145 Reporter dye 55
− Computational 145 Retro-transcribed RNA 301
− Experimental 145 Reverse transcriptase 315
Protein solubilization 156 Reverse Transcriptase-Polymerase
Proteolytic enzyme 76 Chain Reaction (RT-PCR) 123
Proteome 111 Reverse transcription 58
Proteomic 112, 155 Reverse two-hybrid system
− Protein 155 interaction 148
− Protein expression 155 RFLP 238
− Protein map 155 Rhizobium spp 369
Protocol 8 Rhizoplane 364
Pseudomonas 191, 290, 367 Rhizosphere 193, 247, 363, 400
PSI 321 Rhizosphere competence 364
PSII-RC 321 Rhystimatales 9
Pteris ensiormis 308 RIA 96
Purification 83 Ribosomal DNA gene 5
− Affinity chromatography 84 Ribosomal intergenic spacer analysis
− DEAE-Sepharose (RISA) 295
chromatography 83 RNA 113, 193, 301
− Salting-out 83 RNA-dependent RNA polymerase
− Sepharose chromatography 83 (RdRp) 134
PVLG 383 RNA-induced silencing complez
Pyrosequencing 39 (RISC) 134
Pyruvate kinase 277 RNA interference 136
Pythium 2 RNA isolation 57
RNA protein 150
Quartz fibre balance 274 RNase 242
Quelling 134 RNase activity 125
Quencher dye 55 RNase III 135
Quercus robur 250 RNase protection assays 53
Root colonization 384
Radioactivity 115 RsaI 297
Random amplified polymorphic DNA
RAPD 237 Saccharomyces cerevisiae 21, 237
Random mutagenesis 137 Salicylic acid 281
RAPD analysis 244 Salmon sperm 115
rDNA 248 Salting out 83
Reaction centre 325 SAR 344
Real time – ABI prism 7700/7000 61 Scavengers 345
Real time microassays 276 Scorpion probes 54
Real time PCR 54 Scutellospora calospora 381
Real time PCR machines 61 SDS-PAGE 86, 158
Recognition sequence 297 Sebacina 214, 248
Redundant DNA 237 Sebacina aff. epigaea 248
Subject Index 425

Sebacinaceae 248, 355 Spilanthes calva 250

Sebacina dimitica 248 Spinacia oleracea 249
Sebacina epigaea 248 Spinner flask 81
Sebacina incrustans 248 Spinobulbular Muscular atrophy
Sebacinales 308, 345 (SBMA) 141
Sebacina vermifera 213, 248 Spleen cell 79
Sense orientation 137 Split Interaction 148
Sensu stricto 248 Split-ubiquitin 148
Sequence-specific RT primer 69 Spongy parenchyma 271
Setaria italica 250 Spore 3
Shannon–Weaver index 188 Sporophore 238
Short oligonucleotide primer 238 SSCP 4
Shrimp alkaline phosphatase Standard curve method 59
(SAP) 48 Stress tolerance 345
Signal fluorescence 299 Strong terminator peak 48
Signaling genes 18 Structural genes 7
Signaling pathways 18 Suillus bovinus 22
Silencing 133 Suillus granulatus 238
Silico 146 Superoxide dismutase 345
Silver staining method 86 Superscript II reverse
Single tube PCR 59 transcriptase 315
Sirna 135 Surface plasmon resonance 146
SiRNA–mRNA hybrid 136 Systemic disease resistance 348
Sodium azide 90
Sodium thiosulfate 162 TAMRA-500 299
Soil aggregates 188 TAP software 299
Soil amendments 182 Taq 297, 300
Soil environment 156 Taq DNA polymerase 40, 297
Soil environment enzyme TaqI 297
activity 156 Taqman assay 54
Soil environment humic Taq polymerase 171, 241
substance 156 Taqman probes 54
Soil enzyme 199 Target cell 101
Soil enzyme dehydrogenase 208 Targeted analysis 283
Soil enzyme respiration 208 Taxonomy 3
Soil fungi 1 T cell receptor 96
Soil microbe 156 T cytotoxic cell 96
Solanum melongena 250 T cytotoxic cells 96
Sorghum vulgar 250, 386 T-DNA 140
Sos Recruitment system 148 Tectona grandis Linn. 250
Spatial calibration 50 TEMED 88, 219
SPE 284 Template DNA 170
Species identification 10 Template RNA 59
Specific activity 216 Tephrosia purpurea 250
Spike 49 Terminalia arjuna 250
426 Subject Index

Terminal restriction 295 Transposon 134

Terminal restriction fragment TREE program 240
(TRF) 4, 296 Trehalose 278
Terminal – Restriction Fragment Trehalose metabolism
Length Polymorphism 295 neutral trehalase 276
Tetroxide 230 Trehalose phosphate synthase 276
TGGE 4 Tremellales 248
T helper cell 96 Tremelloscypha 214
Therapeutic 141 Tremelloscypha gelatinosa 248
Thermal cycler 241 T-RFLP 295
Thermal stability 118 T-RFLP analysis 295
Thermoplasma acidophilum 358 Trichoderma 1, 239
Thermoplasma volcanium 358 Trichoderma harnazium 8
Thermotoga maritima 358 Tricholoma terreum 238
Thlaspi caerulescens 357 Trifolium alexandrinum 386
Three-hybrid 150 Trypan blue (TB) 100, 382
Three-hybrid bridging factor 150 Trypsin 163
Threshold 62, 71 Tungsten halogen lamp 61
Thymidine 78 Two hybrid system 147
TOC analyser 204 Two-hybrid system interaction 147
Torenia hybrida 140
Traditional systematics 1 UDP glucose 277
Transcribed spacers 7 Uniparentally 237
Transcription activation Universal cycle 41
domain 147 UPGMA cluster analysis 240
Transcription factor 147 Uracil-N-glycosylase (UNG) 71
Transcriptome 111 Urea 156
Transcriptomic 112 UV light 114
Transcript quantification 53 UV rays 241
Transcript quantification UV transilluminator 241
cDNA array 53
Transcript quantification Vacuolar protein sorting 20
in situ hybridization 53 Vector pGADT7-Rec 26
Transcript quantification Vermiculite 388
northern blotting 53 Vibrio fischeri 370
Transcript quantification Viral genome 134
reverse transcription Vitality 337
– polymerase chain reaction 53
Transcript quantification Washing buffer 228
RNase protection assay 53 Western blot 226
Transfer buffer 4,226 Whatman paper 114
Transformation system Withania somnifera 250
for gene replacement 18 Woody Plant Medium
Transilluminator 244 (WPM) 24
Subject Index 427

X-ray film 116 Zea may 250, 380, 386

Xylariales 9 Zinc homeostasis 356
Zizyphus nummularia 250
Yeast two hybrid system 146
Yields 330
YPD medium 151