EX.NO.8 IDENTIFICATION sre FF UNCTIONAL COMPOUND GROUP IN ORGA Date: Aim: To identify the functional groups present in ence of Alcoholic gr est th nent fa. Ceric ammonium nitrate test: | |To the given liquid add few drops of ceric ammonium nitrate soln, given Organic compound. Alcoholic group is present Wine red colouration Add a pellet of KOH to | ml of Presence of recipitate is yellow Dien Alcoholic group is | liquid organic compound and _| formed. quid’ organi confirmed. | warm it to dissolve KOH. Cool i | |itand add few drops of CS, and | shake To test the presence of Phenolic group | S.No. Experiment Observation Inference | P | 1. | Puta pinch of solid compound The colour changes | The given compound is ona piece of moist blue litmus | to red identified to be phenol. paper 2. Ferric Chloride test: Appearance of blue | Phenolic group is Take 2ml of aqueous (or) green violet colour present alcoholic solution of compound | or red colour 5 and add 2-3 drops if'aq, FeCl, No chatacterist Absence of Phenolic solution. colour sense ie 3. Phthalein dye test: Take 0.! i ‘ake 0.2g of organic compound The solution and add equal amount of Phthalic anhydride, Add few drop of conc. H,SO,. Heat for Imin & pour the content in beaker containing 50ml of NaoH Presence Phenolic group is confirmed. becomes pink of solutionst the presence of Aldchydi ind Ket C S.No Experiment Observation Inference 2,4 — DNP test: Take Iml of aqueous or alcoholic solution ofa pinch of compound and add Iml of alcoholic sol of 2-4 dinitropheny! hydrazine. Boil the mixture & cool An Yellowish Orange precipitate is obtained May be presence of Aldehyde (or) Ketone group Schiff’s test: To Iml of the organic sample add 3-4 drops of Schiff’s reagent A pink colour appears. No pink colour appears Presence of aldehyde group Presence of ketone group w Tollen’s test: To the given organic compound add Iml of tollen’s reagent and heat the mixture and cool for 5 minutes. Forrmation of silver mirror on inner sides of test tube No formation of silver mirror Presence of aldehyde group Presence of ketone group Fehling’s Test: To a given soln. add Iml of each Fehling’s solution A & B. Boil the solution for two minutes. A Brick red precipitate is formed No brick red precipitate is formed Presence of aldehyde group Presence of ketone group o Benedict's test: To few ml of organic compound add 2ml of Benedict’s reagent and place the test tube in a boiling water bath for 5 minutes. ‘An orange red precipitate is formed No precipitate is formed Presence of aldehyde group Presence of ketone groupExperiment Observation se Inference Litmus test: To the drop of the liquid compound or solution dip a blue litmus paper or add blue litmus solution If it changes to red May be Phenolic or Carboxylic acid group 2. | Sodium-bi-carbonate te: Presence of — To the given compound or its__| Brisk effervesence of _| Carboxylic acid | aq. solution add solution of CO, is obtained group NaHCO, | Ester formation test. 3. | To the given organic compound, add ethyl alcohol and few drops | of conc. H,SO,,. 2s Pleasant fruity smelling compound is formed Presence of carboxylic acid group is confirmed