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Pepsin Lab Eggs

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Melva Guerra
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27 views5 pages

Pepsin Lab Eggs

Uploaded by

Melva Guerra
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Lab #

Date: ______________________

Topic: Digestion: Enzymes

Aim: To investigate the effect of pepsin, a protease, on albumin in animal cells.

Skills being assessed: AI/MM

Materials:
 6 test tubes  Albumin  Hot plate
 Test tube rack suspension  Thermometer
 Lab burner  1% Pepsin solution  Distilled water
 1M Hydrochloric  Syringe  Biuret reagent
Acid solution  250ml Beaker

Procedure:
Albumin Solution preparation:
1. Separate 1 egg white (albumin) from the yolk and then add 20ml of distilled
water to the albumin.
2. 1% pepsin solution – dissolve 0.1g of powdered pepsin in 9.9ml of distilled
water.
3. Prepare 0.1M hydrochloric acid solution and 1% pepsin solution.

Rate of reaction:
1. Label 5 test tubes as follows: “1”, “2”, “3”, “4” and “5”.
2. Place 5ml of egg white (albumin) suspension into each test tube.
3. Add 3 drops of dilute hydrochloric acid to tubes 3 and 4.
4. Using a syringe, place 1ml of 1% pepsin solution in a clean test tube.
5. Heat the test tube over a small Bunsen flame until the liquid boils.
6. Add the boiled pepsin to the egg white suspension in test tube 5.
7. Fill the 250ml beaker halfway with water. Place the beaker on a hot plate to
prepare a water bath that will sustain a temperature of about 40°C.
8. Add 1ml of 1% pepsin solution to tubes 2 and 4 only.
9. Place all 5 test tubes in the water bath for 5minutes.
10. After 5 minutes, remove the tubes from the water bath and replace them in
the test tube rack. Compare the appearance of contents and then fill in the
table of results.
11. Add 2.oml of biuret reagent to each test tube and invert to mix. Observe the
color of each tube and complete the columns in data table.
Variables:

Dependent – _________________________________________________

Independent – _________________________________________________

Constant – _________________________________________________

Results
Data Table

_____________________________________________________________________________

_____________________________________________________________________________

Biuret Presence/
Test test color absence
Contents Observation
tube # of protein
(√/X)

1 Egg white suspension only

Egg white suspension +


2
pepsin

3 Egg white suspension + HCl

Egg white suspension +


4
pepsin + HCl

Egg white suspension +


5
boiled pepsin
Discussion:
______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________
Limitations:

1. __________________________________________________

2. __________________________________________________

Sources of errors:

1. __________________________________________________

2. __________________________________________________

Conclusion:
______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

Reflection:
______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________

______________________________________________________________________________
Grading Criteria

MM- Use of Thermometer Marks Student


grade
1. Ensure bulb is completely submerged
a) Always 2
b) Most of the times 1
2. Stirs before measuring temperature
a) Always 2
b) Most of the times 1
3. Ensures bulb does not touch the inside of container
a) Always 2
b) Most of the times 1
4. Ensures thermal equilibrium before reading temperature
a) Always 2
b) Most of the times 1
5. Read thermometer perpendicular to scale
a) Always 2
b) Most of the times 1
6. Reads and interpret scale correctly
a) Always 2
b) Most of the times 1
7. Wipes thermometer after use for storage 1
8. Stores thermometer safely after use 1
Total Marks 14
CSEC Marks 10

Profiles Criteria: AI Total Student


Marks Score
Observation Accurately records color changes or appearances 2
of reaction Makes comparison of color changes between test 2
tubes
Makes deduction of the role of pepsin in animals 2
Deductions Gives explanation for differences in observation in 2
from each test tube
observation Identifies the need for boiling
Identifies the need for using HCl 1
Makes accurate deduction on factors affecting 2
pepsin enzyme
Deduces positive and negative tests for proteins 1
Makes accurate conclusion of the factors affecting 2
pepsin
Total Marks 15
CSEC score 10

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