M.sc. - Biotechnology

Total No. of Questions : 3] SEAT No.
:
P554 [Total No. of Pages : 2
[4338]-12
M.Sc. (Semester - I)
BIOTECHNOLOGY
BT - 12 : Cell Biology
(Theory) (2005 Pattern)
Time : 3 Hours] [Max. Marks : 80
Instructions to the candidates:
1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw neat labelled diagrams wherever necessary.
Q1) Answer in short : [20]

a) Describe the term glyoxysome.
b) What is the endosymbiotic theory?
c) How do plasmodesmata help in the transfer of plant viruses?
d) Define the term annulus.
e) Describe anterograde transport in brief.
f) What is the function of proteasomes?
g) Comment on the role of cellulose microfibrils in maintaining turgor
pressure of plant cells.
h) Differentiate between ER cisternae and ER lumen.
i) With suitable examples explain the term transmembrane protein.
j) Discuss the role of the contractile ring in cytokinesis briefly.
Q2) Attempt any two : [30]
a) Write short notes on :
i) Cyclic electron flow and its role in a plant cell.
ii) The role of different molecules in cell cycle arrest at the DNA damage
check points.
b) Answer the following :
i) What is treadmilling? Explain the role of GTP in microtubule
polymerization.
ii) What are the advantages of embryonic stem cells over adult stem
cells in therapeutic cloning?
c) Elucidate the role of the following :
i) Proplastids and Etioplasts in the development of chloroplasts.
ii) APC in separation of sister chromatids.
P.T.O.
Q3) Attempt any three [30]
a) Explain the principle of confocal microscopy and add a note on its use
in Biology.
b) With the help of a diagram explain the death receptor pathway of
apoptosis.
c) How do cytoplasmic structures predict the plane of cell division before
mitosis begins in plant cells?
d) What are the characteristic features of nucleoporins that make up the
nuclear pore complex?
e) Describe the role of pRb as a tumor suppressor protein in regulating cell
cycle.
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[4338] - 12 2
Total No. of Questions : 3] SEAT No. :
P555 [4338]-13
[Total No. of Pages : 2
BIOTECHNOLOGY
BT - 13 : Quantitative Methods
(2005 Pattern)
1) All questions are compulsory.
3) Use of Non - programmable calculator is allowed.
Q1) Attempt the following all subquestions :

a) Explain the FUNCTIONAL BLOCK DIAGRAM of computer. [10]
b) Explain the following terms with suitable example : [5]
i) Population
ii) Sample.
Also explain simple random sampling method.
c) A thermometer reading 75°F is taken out, where the temperature is 20°F.
4 minutes later, the reading is 30°F. Find the thermometer reading 7 minutes
after the thermometer was brought outside. [5]
Q2) Attempt any TWO of the following.

a) i) Explain any two OUTPUT devices. [10]
ii) Compute median and mode for the following data : [5]
Age in years 20-29 30-39 40-49 50-59 60-69 70-79 80-89
No. of patients 55 93 113 90 85 72 34
b) i) What is an OPERATING SYSTEM? Explain its functions. [10]
ii) Estimate the missing term using the relation between Δ & E. [5]
x 0 1 2 3 4
y 1 3 9 81
P.T.O.
c) i) Describe four different NETWORK TOPOLOGIES. [10]
ii) Write the test procedure for testing Independence of two
attributes. [3]
iii) Show that the following differential equation is exact. [2]
(3 xy 2 − x 2 ) dx + (3 x 2 y − 6 y 2 − 1) dy = 0
Q3) Attempt any THREE of the following.

a) i) Describe OSI reference Model in brief. [5]
ii) A random sample of 40 emergency reports was selected from the
files of an ambulance service. The mean time (computed from
sample) required for ambulance to reach their destinations was 13
minutes with standard deviation 3 minutes.
However the ambulance service claims average time to reach
destination 10 minutes.
At 5% level of significance, what is your conclusion? [5]
b) i) Distinguish between RAM and ROM. [5]
ii) Solve the following system of linear equations using matrix inversion :[5]
x + 3y + 3z = 2; x + 4y + 3z = 1; x + 3y + 4z = 3.
c) i) Write a note on UNIX operating system. [4]
ii) Define the following terms : [2]
1) Level of significance.
2) Test statistic.
2 2
Evaluate ∫ x e dx .
3 x
iii) [4]
0
d) i) Explain different services of INTERNET. [4]

ii) In a study of obesity the following results were obtained from
samples of males and females between ages of 20 and 75 years.[4]
No. of observations No. of overweight
Male 150 21
Female 200 48
Can we conclude from these data that in the sampled populations there
is a difference in proportions, who are overweight? (use α = 5%).
dy
iii) Find if, y = e x (2 x 3 − 1) [2]
dx
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[4338]-13 2
Total No. of Questions : 6]
SEAT No. :
[4338] - 21
M.Sc. (Semester - II)
BIOTECHNOLOGY
BT-21 : Molecular Biology
(2005 Pattern)
Time : 3 Hours] [Max. Marks :80
Instructions to the candidates :
1) Question Nos. 1 is compulsory. Out of the remaining attempt 4 questions.
2) Neat diagrams must be drawn wherever necessary.
Q1) a) Give the importance of DNA repair mechanisms. [5]

b) Explain the genetic code used for protein synthesis. [5]
c) Differentiate between the protooncogenes and oncogenes. [5]
d) Explain chromatin remodelling in relation to gene expression. [5]
Q2) a) Give the mechanism of action of DNA Polymerase during replication.

Add a note on the types of polymerases. [8]
b) Discuss the mitochondrial genome with reference to nuclear genome.[7]
Q3) a) How is the mechanism of translation in prokaryotes different from

eukaryote [8]
b) Explain the enzymes involved in DNA modification. Give their importance.
[7]
Q4) Write short notes on : [15]
a) Sequence complexicity in eukaryote
b) Chemical agents causing DNA damage.
c) DNA melting and buoyant density.
P.T.O.
Q5) a) Describe the techniques used to understand DNA - protein interaction.[8]
b) Describe the homeotic gene complexes that determine pattern formation

in Drosophillo. [7]
Q6) a) Define recombination. Give a comparative between homologous and

non - homologous recombination. [8]
b) Explain the structure and mechanism of action of RNA polymerases.[7]
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[4338]-21 2
SEAT No. :
[4338] - 22
BIOTECHNOLOGY
BT - 22 Genetics
(2005 Pattern)
Time : 1½ Hours] [Max. Marks :40
1) Question No. 1 is compulsory. Out of remaining attempt 2 questions.
4) Your answers will be valued as a whole.
Q1) Write short notes on any four of the following [4 × 2.5 = 10]
a) Law of Independent Assortment.
b) Hardy - Weinberg law
c) Polyploidy
d) Incomplete dominance
e) Prototrophs
Q2) a) Explain the control of gene expression in bacteria with arabinose operon.[5]
b) What is linkage? Explain how it can be used for gene mapping. [5]
c) Explain IS elements in bacteria. [5]
Q3) a) Define gene interaction. Explain any one gene interaction with suitable
example. [5]
b) Internal Environment plays an important role in the expression of genes
Discuss with suitable examples. [5]
c) Define linkage and crossing over. Add a note on double crossovers. [5]
Q4) a) Enlist chemical mutagens. Discuss the mechanism of action any typical
mutations caused by any one of them. [5]
b) Define transformation. Explain the formation of competent cells and DNA
transfer by transformation. [5]
c) Explain importance of hybridisation in plant improvement. [5]
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SEAT No. :
[4338] - 23
BIOTECHNOLOGY
BT - 23a : Microbiology
(2005 Pattern)
1) Question No. 1 is compulsory. Out of the remaining attempt 2 questions.
Q1) Explain the growth kinetics involved in the phases of bacterial growth curve.[10]
Q2) a) Describe the crown gall formation mechanism in Agrobacterium. Add a

note on nitrogen fixation. [8]
b) Discuss the Pathogenicity of Mycobacterium tuberculosis. What
precautions should be taken while handling Pathogens. [7]
Q3) Write short notes on. [15]

a) Multiple drug resistance
b) Preservation of bacteria
c) Anaerobes
Q4) a) Explain the physical & chemical methods of sterilization. [5]
b) Describe the method of ethanol production using micro organisms. [5]
c) Comment on the classification and life cycle of cyanobacteria. [5]
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SEAT No. :
[4338] - 24
BIOTECHNOLOGY
BT - 23b : Virology
(2005 Pattern)
1) Question No. 1 is compulsory. Out of the remaining attempt 2 questions.
Q1) Explain the morphology. Ultrastructure and steps involved in replication of a

typical RNA virus. [10]
Q2) a) Explain the laboratory tests used in viral diagnosis. [7]

b) Comment on the candidature of protein and DNA as the vaccine
candidate. [8]
Q3) Write notes on. [15]

a) Vaccine trials
b) Si RNAs
c) Bacteriophages
Q4) a) Describe the method for propagation of animal viruses. [5]
b) Enlist and explain any one method to study the viruses. [5]
c) Give the molecular basis for virus - induced immunodeficiency (AIDS)[5]
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SEAT No. :
[4338] - 25
BIOTECHNOLOGY
BT-24 : Immunology
(2005 Pattern)
1) Question Nos. 1 are compulsory. Out of the remaining attempt 2 questions.
Q1) Give a comparative account on BCR and TCR. Add a note on rδTCR. [10]
Q2) a) Discuss the mechanism of immune response to viral and bacterial

lymphatic infection. [8]
b) Describe the structure and function of antibody. [7]
Q3) Write notes on : [15]

a) Secondary signalling.
b) Hypersensitivity.
c) Autoimmune diseases.
Q4) a) Describe the mechanism of MHC peptide interaction. [5]

b) Explain the B - cell ontology. [5]
c) Explain the major events in the inflammatory responses. [5]
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SEAT No. :
[4338] - 26
BIOTECHNOLOGY
BT - 25 : Bioinformatics
(2005 Pattern)
1) Question Nos. 1 is compulsory. Out of the remaining attempt 2 questions.
Q1) What is sequence Alignment? Give full account of pairwise sequence alignment.
[10]
Q2) a) Write in detail Homology Based Gene Prediction. [5]

b) Write difference between Local & Global Alignment. [5]
c) What is classification of protein? Explain with the help of CATH Topology.[5]

a) BLAST
b) Gen Bank
c) Pattern searching.
Q4) a) Explain in detail Ab initio structure prediction of proteins. Give name of

the tools used in Ab initio prediction. [8]
b) What is Entrez? Explain the information retrieval system by Entrez. [7]
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P562 [4338]-31
M.Sc. (Semester - III)

BIOTECHNOLOGY
BT - 31 : Tissue Culture (Plant and Animal)
(2005 Pattern)
1) Attempt a total of Five questions selecting atleast two questions from each section.
2) Answers to the sections must be written on separate answer books.
SECTION - I
Q1) What is micropropagation? Mention its stages, advantages and limitations.[16]
Q2) Enlist the incubation systems used for plant tissue and cell culture. Explain
any one system. Add a note on its advantages. [16]
Q3) Explain the procedure for obtaining plant protoplasts. Enlist the applications
of isolated plant protoplasts and explain any one. [16]
Q4) Write explanatory notes on any two of the following. [16]
a) Redifferentiation in vitro.
b) Hormonal regulation of in vitro morphogenesis.
c) GM crops
SECTION - II
Q5) Explain advantages and limitations of serum free media in animal cell &
tissue culture. [16]
Q6) How are three dimensional cultures established and maintained? What are
the advantages and applications of three dimensional cultures? [16]
Q7) Explain the basis and procedure of cryopreservation. What are its applications
in animal cell and tissue culture? [16]
Q8) Write explanatory notes on any two of the following. [16]
a) Stem cell culture.
b) Maintenance of cell lines.
c) Cell disaggregation.
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SEAT No. :
[4338] - 32
BIOTECHNOLOGY
BT-32 : Fundamentals of Genetic Engineering
(2005 Pattern)
1) Attempt a total of four questions selecting atleast Two Questions from each
section.
SECTION - I
Q1) What is a cDNA library? Explain the method for construction of cDNA library.
[10]
Q2) What are expression vectors? Mention such vectors in bacteria and describe
salient features of any one such vector. [10]
Q3) Write notes on [10]

a) Site directed mutagenesis.
b) Southern blotting.
SECTION - II
Q4) Explain the method of screeing and selection of transformant. Cite an
appropriate example. [10]
Q5) Enlist the methods of transformation in vitro. Explain any one method to
obtain genetically transformed plant cells. [10]
Q6) Write notes on. [10]

a) YAC
b) Chimeric constructs.
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SEAT No. :
[4338] - 33
BIOTECHNOLOGY
BT-33 : Biological Chemistry - II
(2005 Pattern)
Section.
2) Answers to the Sections must be written on separate answer books.
SECTION - I
Q1) What is electrophoresis? Mention its types and explain any one. [10]
Q2) Explain the use of ion exchange chromatography for purification of proteins.
[10]

a) Ramchandran plot.
b) Protein sequencing
SECTION - II
Q4) Explain the salient features of tertiary structure of proteins. [10]
Q5) How is differential centrifugation employed for cell fractionation? Add a note
on isolation of any one cell fraction. [10]

a) MALDI. TOF
b) Micro arrays.
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SEAT No. :
[4338] - 34
BIOTECHNOLOGY
BT-34 : Biochemical Engineering
(2005 Pattern)
section.
SECTION - I
Q1) Compare advantages and limitations of mechanically agitated and
nonmechanically agitated fermenters. [10]
Q2) Explain the process of gas liquid mass transfer of oxygen. [10]

a) Bioprocess scale up.
b) Heat transfer mechanism in bioreactors.
SECTION - II
Q4) Enlist the types of aerators used for bioprocesses. Explain the function of any
one. [10]
Q5) How do shear stress and shear rate affect the broth rheology? [10]

a) Types of valves in a bioreactor.
b) Reynolds number.
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SEAT No. :
[4338] - 35
BIOTECHNOLOGY
BT - 35 : Pleuripotent Cell Technologies and Reproduction
(2005 Pattern)
1) Attempt a total of four questions selecting atleast Two questions from each
section.
SECTION - I
Q1) What is oogenesis? Explain the nucleo cytoplasmic changes associated with
it. [10]
Q2) Explain the role of primary embryonic induction in the process of cell
differentiation. [10]

a) Embryonal stem cells.
b) Transgenic animals.
SECTION - II
Q4) Enlist the techniques used in development of transgenic animals and explain
any one. [10]
Q5) Explain the cellular and subcellular processes involved in pattern formation in
Drosophila embryo. [10]

a) Salient features of adult stem cells.
b) Gene therapy.
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SEAT No. :
[4338] - 101
BIOTECHNOLOGY
BT-11 : Advanced Biological Chemistry
(2008 Pattern)
1) Attempt not more than 5 questions of which at least 2 questions must be from
each section.
2) Answers to the two sections should be written in separate books.
5) All questions carry equal marks.
SECTION - I
Q1) a) What are biological buffers? Explain bicarbonate system for maintaining
acid - base balance. [8]
b) Give principle and application of ISO - electric focussing. [8]
Q2) a) Differentiate between analytical and preparative centrifugation with suitable

example. [8]
b) Explain the term metabolic engineering with representative example. [8]
Q3) a) Discuss the physical basis of peak broadening in chromatography with

example. [8]
b) Give the types of secondary metabolites of plant origin. State the use of
any one type in agriculture. [8]
a) Electroendoosmosis.
b) Metabolic flux analysis.
c) Chemical shift.
d) Redox potential.
P.T.O.
SECTION - II
Q5) Describe the non covalent forces that control the protein structure with
illustrative example. [16]
Q6) a) Discuss the importance of multienzyme complexes in natural product

biosynthesis. [8]
b) Describe Jacques Monads (MWC) model for allosteric transitions of
protein. [8]
Q7) a) Enlist the types of terpenoids and give the steps involved in biosynthesis
of terpenoids. [8]
b) Explain the role of proteins in membrane organisation. [8]

a) Use of herbals in medicine.
b) Protein engineering.
c) Chow - Fasman method.
d) Pharmacological activities of phenolics.
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[4338]-101 2
SEAT No. :
[4338] - 102
BIOTECHNOLOGY
BT - 12 : Molecular and Cell Biology
(2008 Pattern)
1) Attempt not more than 5 questions of which at least 2 questions must be from
each section.
5) All questions carry equal marks.
SECTION - I
Q1) a) Enlist the major differences between oxidative phosphorylation and photo
phosphorylation. [8]
b) What are the major features of F1 F0 ATPase complex? [8]
Q2) a) Describe in detail the modulation of cytosolic Ca++. [8]

b) Highlight the role of inositol - 1, 4, 5 triphosphate in Ca signaling pathway.[8]
++
Q3) a) Describe the external respiration. [8]

b) What are the factors on which efficient external respiration depends upon?[8]
Q4) a) Describe the physiological and metabolic effects of epinephrine. [8]
b) How does skin contributes to the regulation of body temperature? [8]

SECTION - II
Q5) a) What do you understand by differential gene expression? [8]
b) How does the environmental factors influence expression of gene? [8]
Q6) a) What are interferons? Describe the cellular process involved in Innate
defense mechanism. [8]
b) Describe natural defense against diseases in animals. [8]
P.T.O.
Q7) a) What is the role of gene promoter like TATA boxes? [8]
b) How does the machinery of the cell know where to begin reading the
gene. [8]
Q8) a) Ribosomes are the work benches for translation. Justify the statement.[8]
b) Describe the role of genetic variation and natural selection in evolution.[8]
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[4338]-102 2
SEAT No. :
[4338] - 103
BIO - TECHNOLOGY
BT-13 : Environmental Biotechnology
(2008 Pattern)
1) Attempt a total of five questions selecting atleast two questions from each section.
2) Answers to the sections must be written in separate answer books.
SECTION - I
Q1) What do you understand by non - conventional energy sources? Discuss the
advantages, limitations and applications of geothermal energy. [16]
Q2) Write explanatory notes on : [16]
a) Use of genetically modified plants against pathogens.
b) Transport and diffusign of air pollutants.
Q3) Describe any one technique used in the measurement of noise pollution. Discuss
various ways for control and abatement of noise pollution. [16]
Q4) Describe in detail the process of biodegradation of fungicides and pesticides
in soil. [16]
SECTION - II
Q5) Explain in detail various strategies for phytoremediation. Give appropriate
examples. Add a note on its applications. [16]
Q6) a) Explain the importance of flow and loading rate in biological waste water
treatment. [8]
b) Explain advanced waste water treatment. [8]
Q7) What is the scope of conservation biotechnology? Explain the use of
biotechnology - based methods for microbial conservation. [16]
Q8) Write explanatory notes on : [16]
a) Minas for industries and Ecomarks.
b) ISO 14000 standard series.
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P570 [4338]-201

BIOTECHNOLOGY
BT - 21 : Genetic Engineering
(2008 Pattern)
1) Attempt not more than 5 questions of which at least 2 questions must be from each
section.
SECTION - I
a) Regulation of copy number in plasmids.
b) Chimeric construct.
c) Synthetic promoters used in expression vectors.
d) Construction of cDNA library.
Q2) a) Comment on the difference in strategies used for cloning prokaryote and
eukaryote genes. [8]
b) Explain the mechanism and applications of lac operon in expression of
industrially important products. [8]
Q3) a) Describe the methods used for screening and selection of transformed
cells in gene library construction. [8]
b) i) Give the importance of topoisomerase & ligase enzyme.
ii) With suitable example describe a typical yeast expression vector.
[8]
Q4) a) Give a comparative account between plasmid and phage as DNA carrier with
respect to host, insert size, entry in host, efficiency and application. [8]
P.T.O.
b) i) Write a brief note on restriction enzymes, its types and role in genetic
modification.
ii) Construct a restriction map for a 8.9 kb circular plasmid which is
singly and doubly digested with 3 restriction enzymes, EcoRI, Bam
HI and Hind III.
Following bands were obtained :
Eco RI 8.9 kb
Bam HI 6 kb & 2.9 kb
Hind III 8.9 kb
EcoRI + Bam HI 6.0 kb, 2.4 kb & 0.5 kb
EcoRI + Hind III 7.4 kb & 1.5 kb
Bam HI + Hind III 5 kb, 2.9 kb & 1 kb
EcoRI + Bam HI + Hind III 5 kb, 2.4 kb, 1 kb, & 0.5 kb [8]
SECTION - II
Q5) a) What are Co - dominant markers? Explain the development of
miciosatellite markers. [8]
b) Explain the technology used for creating the cloned sheep Dolly. Give,
reason for early death of Dolly at the age of 6 (normal sheeps live upto to
12 years). [8]
Q6) Write an assay on any two methods employed for transgenic plant
development. Add a note on biosafety precautions taken while releasing
transgenic plants. [16]
Q7) a) Explain the recipe for a typical polymerase chain reaction, commenting
on the importance of each ingredient. [8]
b) Give the principle of Sangers di - deoxy method for sequencing a gene.
How automation in this technology been revolutionerised. [8]

a) Multiplex PCR.
b) Fusion proteins.
c) Cre - lox technology.
d) Amplified Fragment Length Polymorphism.
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[4338]-201 2
P571 [4338]-202

BIOTECHNOLOGY
BT - 22 : Bioinformatics
(2008 Pattern)
1) Attempt not more than 5 questions of which at least 2 questions must be from each
section.
SECTION - I
a) Genetic algorithm.
b) PAM.
c) SMILE notation.
d) Secondary data resources.
Q2) a) What are motifs. Write in detail about sequential and structural motif
with one example each. [8]
b) Using the BLAST tool we can find the homology between related
organisms. Justify. [8]
Q3) a) Define gene expression informatics. Give the method and application of
microarray in gene expression analysis. [8]
b) Give an account on progressive and non - progressive methods of multiple
sequence alignment. [8]
Q4) Explain structure based drug designing using chemoinformatics. [16]
P.T.O.
SECTION - II
Q5) a) Give a comparative account between SCOP and CATH. [8]
b) How do you derive a Ramchandran plot. Give its applications in protein
structure validation. [8]
Q6) a) What is epitope. Explain the methods used to predict the epitope using
in - silico approach. [8]
b) Comment on the different routes to funding for research in India and
world wide. [8]
Q7) a) Define structural bioinformatics. Discuss the importance of 3D structure

prediction of protein. [8]
b) Explain the method and applications of microarray in gene expression
analysis. [8]
Q8) Write short note on : [16]

a) Bioinformatics business model.
b) Structure - Function relationship in protein.
c) Substitution matrices.
d) Chou - Fasman algorithm.
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[4338]-202 2
P572 [4338]-203

BIOTECHNOLOGY
BT-23 : Plant Biotechnology
(2008 Pattern)
2) Answers to the two sections must be written on separate answer books.
SECTION - I
Q1) With a suitable example explain the methods used for increased production
of secondary metabolites in vitro using plant cell cultures. Give the significance
of in vitro method over the in viva. [16]
Q2) a) Comment on the methods used for seed improvement, testing and
certification. [8]
b) Explain how clonal variations has helped the crop improvement
programme using plant tissue culture technique. [8]
Q3) a) Explain qualitative and qvantitative improvement of economically

important fungi using modern techniques. [8]
b) Give the traditional uses of algae. Discuss use of biotechnology for mass
propagation of commercially important algal species. [8]
Q4) Write short notes on :

a) Plant growth regulators.
b) Somatic embryogenesis.
c) Biodiesel
d) Haploid production.
SECTION - II
Q5) a) Explain the biolistic - mediated DNA transfection of monocot plants for
pest resistant transgenic plant. [8]
b) Give the importance of use of biofertilizers and vermiculture in modern
agriculture practices. [8]
P.T.O.
Q6) What is plant transformation. With suitable example, explain the direct and indirect
methods of DNA transfer to plants to produce the transgenic plants. [16]
Q7) a) Define the term molecular farming? Explain how plants can be used for
production of pharmaceutical and cosmaceutical products. [8]
b) Describe the process of protoplast isolation, fusion and regeneration of
plants. [8]
a) Single cell proteins.
b) Mass multiplication of forest trees.
c) Direct embryogenesis.
d) Applications of plant biotechnology in Agriculture.
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[4338] - 203 2
SEAT No. :
[4338] - 301
BIOTECHNOLOGY
BT - 31 : Animal Biotechnology
(2008 Pattern)
1) Attempt five questions selecting atleast two questions from each section.
2) Answers to the sections must be written on separate answer sheets.
SECTION - I
Q1) Write short notes : [16]
a) Factors governing female fertility.
b) Semen sexing technology.
Q2) Write briefly about : [16]

a) In vitro culture of embryos
b) Endocrinology of estrous cycle in animals.
Q3) a) Define two types of cell lines and how cell lines are characterized?
b) What test are carried out to find out breeding potential of males in large
animals?
[16]
Q4) a) How embryonic stem cells are established and characterized?
b) Explain in vitro neoplastic transformation phenomenon.
[16]
P.T.O.
SECTION - II
Q5) Explain different methods for developing transgenic small laboratory animals?
Give applications of such animals? [16]

a) Production of Insulin in milk.
b) Cre Lox system for gene transfer.
Q7) a) What is germ cell storage? Give one protocol how it is done.
b) Explain the concept of gene banking and its advantages.
[16]

a) Transgenic animals in meat production
b) Retrovirus mediated gene transfer.
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[4338]-301 2
SEAT No. :
[4338] - 302
BIOTECHNOLOGY
BT - 32 : Fermentation Technology
(2008 Pattern)
1) Attempt a total of five questions selecting atleast two questions from each
section.
2) Answers to the two sections must be written in separate books.
SECTION - I
Q1) a) Discuss mass transfer by molecular diffusion in a fermenter. [8]
b) Discuss the kinetics of product formation by microbial culture in terms
of growth linked products in batch and fed batch cultures. [8]
Q2) a) Explain why biotransformation is widely seen as Green alternative over

conventional chemical routes. Outline the difference between cell and
enzyme based biotransformation.
b) What is measure of volumetric oxygen transfer rate in a fermenter? How
KLa is derived in a fermenter.
[16]
Q3) a) Describe significance of membrane filtration over centrifugation.
b) Discuss role of bio - control agents in integrated pest management. What
are challenges in terms of acceptance of the same?
[16]
Q4) a) Explain isolation of products of biotransformation with suitable example.
b) What is Reynolds number? How it can be useful for to characterize
fluid flow?
[16]
P.T.O.
SECTION - II
Q5) a) Which separation method is suitable for purification of monoclonal
antibodies? What are the advantages of affinity chromatography over
size exclusion chromatography?
b) Discuss different physical and chemical sensors in a fermenter?
[16]
Q6) Outline downstream processing steps for the recovery of recombinant vaccine
and Penicillin. [16]
Q7) Discuss genetic and metabolic pathway engineering as mean for microbial
strain improvement. [16]
Q8) Describe design of a fermenter suitable for cultivation of mammalian cells and
why? Explain immobilized cell reactors and hollow fiber bioreactors. [16]
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[4338]-302 2
SEAT No. :
[4338] - 303
BIOTECHNOLOGY
BT - 33a : Principles of Virology
(2008 Pattern)
1) Attempt four questions selecting atleast Two Questions from each section.
2) Answers to the sections must be written on separate answer sheets.
SECTION - I
Q1) Write short notes [10]
a) Plaque assay
b) HIV genome structure
Q2) a) How disease progression is monitored using polymerase chain reaction?

b) What is mode of action of Acyclovir?
[10]
Q3) Give details of lytic cycle of Lambda bacteriophage. [10]

SECTION - II
a) Persistent viral infections.
b) Foot and Mouth disease.
Q5) How HIV epidemiology is studied and how it can be effectively used for
control of spread of HIV? [10]

a) Mosquito borne new emerging viral diseases.
b) Mareks disease.
tttt
SEAT No. :
[4338] - 304
BIOTECHNOLOGY
BT-33 b : Advanced Immunology
(2008 Pattern)
1) Attempt four questions selecting atleast Two from each section.
2) Answers to the sections must be written in separate answer sheets.
SECTION - I
Q1) a) Which are different hematopoetic growth factors that play role in immune system?
b) Give detailed information of structure and function of T cells including
its surface markers.
[10]
Q2) a) How innate immunity prevents infections in animals?
b) Give structural details of spleen.
[10]
Q3) a) Describe immunity in Drosophila.

b) Give concise account of alternative pathway of compliment fixation.
[10]
SECTION - II
a) Treatment of autoimmune disorder.
b) Large scale cultivation of hybridoma cells.
Q5) Give detailed accounts of humanized antibody preparation. [10]
Q6) a) How antibodies are used as therapeutic agents?

b) With suitable examples describe active immunization.
[10]
tttt
SEAT No. :
[4338] - 401
M.Sc. (Semester - IV)
BIO - TECHNOLOGY
BT-41 : Genomics and Proteomics
(2008 Pattern)
SECTION - I
Q1) Discuss merits and demerits of shotgun sequencing and clone by clone
approach used for whole genome analysis. [12]
Q2) What is comparative genomics? How is it useful in genome annotation? [12]
Q3) Give a brief account of any one of the following. [12]
a) ENCODE project.
b) Use of RNAi in functional genomics.
Q4) Write notes on any two of the following [12]
a) Mutagenesis in functional genomics.
b) Transcriptomics
c) SNP microarrays
SECTION - II
Q5) Explain the use of yeast 2 - hybrid system in functional proteomics. [12]
Q6) Explain any one technique used in structural proteomics. [12]
Q7) What are the methods used for identification and characterization of novel
proteins? Explain any one method. [12]
Q8) Write notes on any two of the following [12]
a) Application of proteomics in drug development.
b) Proteomics in screening of diagnostic markers.
c) Protein - protein interactions a computational approach.
tttt
SEAT No. :
[4338] - 402
BIO - TECHNOLOGY
BT - 42 : Legal and Ethical Aspects in Biotechnology and IPR
(2008 Pattern)
SECTION - I
Q1) What is intellectual property? How is it different from other type of property?
Mention various forms of IPR? [12]
Q2) Mention basic requirements for patenting an invention. Explain with the help
of an appropriate example. [12]
Q3) a) State the rights of a patentee. [6]
b) How are software programs protected? [6]
a) Protection of plant varieties.
b) Protection of farmers rights.
SECTION - II
Q5) State the major changes in Indian patent system in post TRIPS. Explain any
one change. [12]
Q6) Explain the specifications to be provided for filing a patent in Biotechnology.
Cite an appropriate example. [12]
Q7) Explain procedures for :
a) Patenting a biological product. [6]
b) Obtaing a process patent. [6]
a) Geographical Indications.
b) Commercial exploitation of Industrial designs.
tttt
SEAT No. :
[4338] - 403
BIOTECHNOLOGY
BT-43 : Clinical Research and Data Base Management
(2008 Pattern)
section.
SECTION - I
Q1) Explain organisation, responsibilities and authority of FDA. [10]
Q2) Mention at least three medical devices. Explain R and D activities with reference
to any one device. [10]
Q3) Write notes on any two of the following : [10]

a) Importance of GMPs in large scale pharmaceutical production.
b) Marketing the herbal drugs.
c) Research and development of Biologics.
SECTION - II
Q4) What is a database? Mention important fields of information of a database for
clinical research. Add a note on management of clinical database. [10]
Q5) Explain the steps involved in designing the clinical trials. Add a note on
importance of clinical trials. [10]
Q6) Write notes on any two of the following : [10]

a) Query resolution.
b) Protection of safety of human subjects.
c) GLPs for manufacture of pharmaceuticals.
tttt
SEAT No. :
[4338] - 404
BIOTECHNOLOGY
BT - 44 a : Nanobiotechnology
(2008 Pattern)
1) Attempt a total of four questions selecting atleast Two questions from each
section.
SECTION - I
Q1) Explain with the help of appropriate examples, contribution of nanomaterials
in Bioscience and Biotechnology. [10]
Q2) a) Mention the characteristic physico - chemical properties acquired by the

materials due to nanosize. [5]
b) Justify any two biomolecules as nanostructures. [5]

a) Thin films and their application in biotechnology.
b) Gene therapy.
SECTION - II
Q4) Explain one in vivo and one in vitro method of synthesis of nanostructures.[10]
Q5) How are nanoparticles functionalized for biological application? Explain with
an appropriate example. [10]

a) Nanobiotechnology in pharmacokinetics.
b) Nanowires and their application.
tttt
SEAT No. :
[4338] - 405
BIOTECHNOLOGY
BT - 44 b : Stem Cell Technology and Regenerative Medicines
(2008 Pattern)
SECTION - I
Q1) What is pattern formation? Mention developmental events with respect to any
two patterns. [12]
Q2) Explain the subcellular events that lead to the differentiation of animal sperm
cell. Add a note on the ultrastructure of the sperm. [12]
Q3) Explain the role of metabolic activation and cytoplasmic rearrangement in
early development after zygote formation. [12]
a) Significance of cell lineages
b) Cell differentiation
SECTION - II
Q5) What is the scope and application of embryonic stem cell technology? Explain
with an appropriate example. [12]
Q6) How are transgenic animals produced? Comment on the advantages and
disadvantages of transgenic animals. [12]
Q7) Discuss possible advantages and ethical problems of human cloning. [12]
a) Advantages and limitations of gene therapy.
b) Knock outs.
tttt
SEAT No. :
[4338] - 406
BIOTECHNOLOGY
BT - 44C : Agricultural Biotechnology
(2008 Pattern)
SECTION - I
Q1) Explain the technique of embryo rescue and its application in agrobiotechnology.
[12]
Q2) What is somaclonal variation? How are somaclonal variants obtained? What
are their applications? [12]
Q3) Compare and contrast advantages and limitations of micropropagation and
conventional vegetative propagation. [12]
a) Induced polyembryony
b) Use of haploids in agriculture
SECTION - II
Q5) What are transgenic plants? Mention their applications and explain any one.[12]
Q6) a) How is gametoclonal variation used for crop improvement? [6]
b) Describe any two methods to test fidelity of progeny raised through
micro propagation. [6]
Q7) Explain the concept of metabolic engineering. Add a note on advantages and
limitations of metabolic engineering. [12]
a) Importance of apomicts in agrobiotechnology.
b) Biopesticides
tttt

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Total No. of Questions : 3] SEAT No.

Q1) Answer in short : [20]

Q1) Attempt the following all subquestions :

Q2) Attempt any TWO of the following.

Q3) Attempt any THREE of the following.

d) i) Explain different services of INTERNET. [4]

Q1) a) Give the importance of DNA repair mechanisms. [5]

Q2) a) Give the mechanism of action of DNA Polymerase during replication.

Q3) a) How is the mechanism of translation in prokaryotes different from

a) Sequence complexicity in eukaryote

b) Chemical agents causing DNA damage.

c) DNA melting and buoyant density.

b) Describe the homeotic gene complexes that determine pattern formation

Q6) a) Define recombination. Give a comparative between homologous and

b) Explain the structure and mechanism of action of RNA polymerases.[7]

Q2) a) Describe the crown gall formation mechanism in Agrobacterium. Add a

Q3) Write short notes on. [15]

Q4) a) Explain the physical & chemical methods of sterilization. [5]

b) Describe the method of ethanol production using micro organisms. [5]

c) Comment on the classification and life cycle of cyanobacteria. [5]

Q1) Explain the morphology. Ultrastructure and steps involved in replication of a

Q2) a) Explain the laboratory tests used in viral diagnosis. [7]

Q3) Write notes on. [15]

Q4) a) Describe the method for propagation of animal viruses. [5]

c) Give the molecular basis for virus - induced immunodeficiency (AIDS)[5]

1) Question Nos. 1 are compulsory. Out of the remaining attempt 2 questions.

2) Neat diagrams must be drawn wherever necessary.

3) Figures to the right indicate full marks.

Q2) a) Discuss the mechanism of immune response to viral and bacterial

Q3) Write notes on : [15]

Q4) a) Describe the mechanism of MHC peptide interaction. [5]

1) Question Nos. 1 is compulsory. Out of the remaining attempt 2 questions.

2) Neat diagrams must be drawn wherever necessary.

3) Figures to the right indicate full marks.

Q2) a) Write in detail Homology Based Gene Prediction. [5]

Q3) Write short notes on : [15]

Q4) a) Explain in detail Ab initio structure prediction of proteins. Give name of

M.Sc. (Semester - III)

Q3) Write notes on [10]

Q6) Write notes on. [10]

Q3) Write notes on : [10]

Q6) Write notes on : [10]

Q3) Write notes on [10]

Q6) Write notes on [10]

Q3) Write notes on : [10]

Q6) Write notes on : [10]

Q2) a) Differentiate between analytical and preparative centrifugation with suitable

Q3) a) Discuss the physical basis of peak broadening in chromatography with

Q4) Write short notes on : [16]

b) Metabolic flux analysis.

Q6) a) Discuss the importance of multienzyme complexes in natural product

Q8) Write short notes on : [16]

Q2) a) Describe in detail the modulation of cytosolic Ca++. [8]

Q3) a) Describe the external respiration. [8]

Q4) a) Describe the physiological and metabolic effects of epinephrine. [8]

b) How does skin contributes to the regulation of body temperature? [8]

M.Sc. (Semester - II)

Q8) Write short notes on : [16]

M.Sc. (Semester - II)

Q4) Explain structure based drug designing using chemoinformatics. [16]

d) i) Explain different services of INTERNET. [4]

Q2) a) Write in detail Homology Based Gene Prediction. [5]