Pseudalert Test

250 mL Enumeration Protocol Addendum

and MPN Table
Follow the procedures in this protocol addendum when using the Pseudalert* Test for the enumeration of
Pseudomonas aeruginosa in 250 mL water samples. Refer to the printed package insert for test storage
requirements and all other procedures.

Quanti-Tray enumeration procedure (250 mL samples only)

1. Divide the 250 mL sample into three sterile, transparent vessels with two samples having aliquots of 100 mL
and one sample having an aliquot of 50 mL.
2. Add 50 mL of sterile, noninhibitory, oxidant-free water to the 50 mL sample aliquot to bring the volume to 100 mL.
3. Aseptically add the contents of a single 100 mL snap pack of Pseudalert* reagent to each of the three vessels.
4. Cap vessels and shake until contents are dissolved.
5. Add 2 drops of IDEXX Antifoam Solution to each sample/reagent mixture.
Note: IDEXX 120 mL sample vessels containing antifoam are also available.
6. Pour each sample/reagent mixture into a separate Quanti-Tray* tray, and then seal with the Quanti-Tray* Sealer;
label the three trays to differentiate between the undiluted and diluted mixtures.
7. Place the sealed trays in an incubator at 38°C ±0.5°C for 24 to 28 hours.
8. Read results according to the result interpretation table below, counting the number of positive wells.
9. Refer to the Pseudalert 250 Quanti-Tray MPN Table on the next page. The most probable number (MPN) is
obtained using the sum of the positive counts from the two undiluted Quanti-Trays and the positive count from
the diluted Quanti-Tray.

Result interpretation
Appearance Result
No blue fluorescence Negative for Pseudomonas aeruginosa

Blue fluorescence† Positive for Pseudomonas aeruginosa

†Greater than the blue fluorescence present in a negative control sample

• Look for blue fluorescence with a 6-watt, 365 nm UV light held within 5 inches of the sample in a dark
environment. Face the light away from your eyes and toward the sample.
• Refer to the –/+ fluorescence reading guide on the test box. The colors are as seen under a UV light.
• Pseudalert results are definitive at 24–28 hours.
 Pseudalert 250 Quanti-Tray
MPN Table
Number of positive wells—both undiluted trays
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

0 <1 1.0 2.0 3.0 4.1 5.1 6.1 7.2 8.3 9.3 10.4 11.5 12.6 13.7 14.8 16.0 17.1 18.2 19.4 20.6 21.8 22.9 24.1 25.4 26.6 27.8

1 1.0 2.0 3.0 4.1 5.1 6.1 7.2 8.2 9.3 10.4 11.5 12.6 13.7 14.8 15.9 17.1 18.2 19.4 20.5 21.7 22.9 24.1 25.3 26.5 27.8 29.0

2 2.0 3.0 4.0 5.1 6.1 7.2 8.2 9.3 10.4 11.5 12.5 13.7 14.8 15.9 17.0 18.2 19.3 20.5 21.7 22.8 24.0 25.2 26.5 27.7 28.9 30.2

3 3.0 4.0 5.1 6.1 7.2 8.2 9.3 10.3 11.4 12.5 13.6 14.7 15.9 17.0 18.1 19.3 20.4 21.6 22.8 24.0 25.2 26.4 27.6 28.9 30.1 31.4

4 4.0 5.1 6.1 7.1 8.2 9.3 10.3 11.4 12.5 13.6 14.7 15.8 16.9 18.1 19.2 20.4 21.6 22.7 23.9 25.1 26.3 27.6 28.8 30.1 31.3 32.6

5 5.0 6.1 7.1 8.2 9.2 10.3 11.4 12.5 13.6 14.7 15.8 16.9 18.0 19.2 20.3 21.5 22.7 23.9 25.1 26.3 27.5 28.7 30.0 31.2 32.5 33.8

6 6.1 7.1 8.2 9.2 10.3 11.4 12.4 13.5 14.6 15.8 16.9 18.0 19.2 20.3 21.5 22.6 23.8 25.0 26.2 27.4 28.7 29.9 31.2 32.4 33.7 35.0

7 7.1 8.1 9.2 10.3 11.3 12.4 13.5 14.6 15.7 16.8 18.0 19.1 20.3 21.4 22.6 23.8 25.0 26.2 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.2

8 8.1 9.2 10.2 11.3 12.4 13.5 14.6 15.7 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.9 26.1 27.3 28.5 29.8 31.0 32.3 33.6 34.8 36.1 37.5

9 9.2 10.2 11.3 12.4 13.5 14.5 15.7 16.8 17.9 19.0 20.2 21.3 22.5 23.7 24.9 26.1 27.3 28.5 29.7 31.0 32.2 33.5 34.8 36.1 37.4 38.7

10 10.2 11.3 12.3 13.4 14.5 15.6 16.7 17.9 19.0 20.1 21.3 22.4 23.6 24.8 26.0 27.2 28.4 29.6 30.9 32.1 33.4 34.7 36.0 37.3 38.6 39.9

11 11.2 12.3 13.4 14.5 15.6 16.7 17.8 18.9 20.1 21.2 22.4 23.6 24.7 25.9 27.1 28.4 29.6 30.8 32.1 33.3 34.6 35.9 37.2 38.5 39.8 41.2

12 12.3 13.4 14.5 15.6 16.7 17.8 18.9 20.0 21.2 22.3 23.5 24.7 25.9 27.1 28.3 29.5 30.7 32.0 33.2 34.5 35.8 37.1 38.4 39.7 41.1 42.4

13 13.3 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.5 24.6 25.8 27.0 28.2 29.4 30.7 31.9 33.2 34.4 35.7 37.0 38.3 39.6 41.0 42.3 43.7

14 14.4 15.5 16.6 17.7 18.8 20.0 21.1 22.2 23.4 24.6 25.8 27.0 28.2 29.4 30.6 31.8 33.1 34.4 35.6 36.9 38.2 39.5 40.9 42.2 43.6 44.9

15 15.5 16.6 17.7 18.8 19.9 21.1 22.2 23.4 24.5 25.7 26.9 28.1 29.3 30.5 31.8 33.0 34.3 35.5 36.8 38.1 39.4 40.8 42.1 43.5 44.8 46.2

16 16.5 17.6 18.7 19.9 21.0 22.2 23.3 24.5 25.6 26.8 28.0 29.2 30.5 31.7 32.9 34.2 35.5 36.7 38.0 39.3 40.7 42.0 43.4 44.7 46.1 47.5

17 17.6 18.7 19.8 21.0 22.1 23.3 24.4 25.6 26.8 28.0 29.2 30.4 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.6 46.0 47.4 48.8
Number of positive wells—diluted tray

18 18.7 19.8 20.9 22.1 23.2 24.4 25.5 26.7 27.9 29.1 30.3 31.6 32.8 34.0 35.3 36.6 37.9 39.2 40.5 41.8 43.1 44.5 45.9 47.2 48.6 50.1

19 19.7 20.9 22.0 23.2 24.3 25.5 26.7 27.8 29.0 30.3 31.5 32.7 34.0 35.2 36.5 37.8 39.1 40.4 41.7 43.0 44.4 45.7 47.1 48.5 49.9 51.3

20 20.8 22.0 23.1 24.3 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.9 35.1 36.4 37.7 39.0 40.3 41.6 42.9 44.3 45.6 47.0 48.4 49.8 51.2 52.7

21 21.9 23.1 24.2 25.4 26.5 27.7 28.9 30.1 31.3 32.6 33.8 35.1 36.3 37.6 38.9 40.2 41.5 42.8 44.2 45.5 46.9 48.3 49.7 51.1 52.5 54.0

22 23.0 24.2 25.3 26.5 27.7 28.9 30.1 31.3 32.5 33.7 35.0 36.2 37.5 38.8 40.1 41.4 42.7 44.1 45.4 46.8 48.2 49.6 51.0 52.4 53.8 55.3

23 24.1 25.3 26.4 27.6 28.8 30.0 31.2 32.4 33.7 34.9 36.2 37.4 38.7 40.0 41.3 42.6 44.0 45.3 46.7 48.0 49.4 50.8 52.3 53.7 55.1 56.6

24 25.2 26.4 27.5 28.7 29.9 31.1 32.3 33.6 34.8 36.1 37.3 38.6 39.9 41.2 42.5 43.8 45.2 46.5 47.9 49.3 50.7 52.1 53.6 55.0 56.5 57.9

25 26.3 27.5 28.7 29.9 31.1 32.3 33.5 34.7 36.0 37.2 38.5 39.8 41.1 42.4 43.7 45.1 46.4 47.8 49.2 50.6 52.0 53.4 54.9 56.3 57.8 59.3

26 27.4 28.6 29.8 31.0 32.2 33.4 34.7 35.9 37.2 38.4 39.7 41.0 42.3 43.6 45.0 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.2 57.6 59.1 60.6

27 28.5 29.7 30.9 32.1 33.3 34.6 35.8 37.1 38.3 39.6 40.9 42.2 43.5 44.9 46.2 47.6 48.9 50.3 51.7 53.1 54.6 56.0 57.5 59.0 60.5 62.0

28 29.7 30.9 32.1 33.3 34.5 35.7 37.0 38.3 39.5 40.8 42.1 43.4 44.8 46.1 47.5 48.8 50.2 51.6 53.0 54.4 55.9 57.3 58.8 60.3 61.8 63.3

29 30.8 32.0 33.2 34.4 35.7 36.9 38.2 39.4 40.7 42.0 43.3 44.7 46.0 47.3 48.7 50.1 51.5 52.9 54.3 55.7 57.2 58.7 60.2 61.7 63.2 64.7

30 31.9 33.1 34.3 35.6 36.8 38.1 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.6 50.0 51.3 52.7 54.2 55.6 57.0 58.5 60.0 61.5 63.0 64.5 66.1

31 33.1 34.3 35.5 36.7 38.0 39.3 40.5 41.8 43.1 44.4 45.8 47.1 48.5 49.8 51.2 52.6 54.0 55.5 56.9 58.4 59.8 61.3 62.8 64.4 65.9 67.5

32 34.2 35.4 36.7 37.9 39.2 40.4 41.7 43.0 44.3 45.7 47.0 48.3 49.7 51.1 52.5 53.9 55.3 56.8 58.2 59.7 61.2 62.7 64.2 65.7 67.3 68.9

33 35.3 36.6 37.8 39.1 40.3 41.6 42.9 44.2 45.5 46.9 48.2 49.6 51.0 52.4 53.8 55.2 56.6 58.1 59.5 61.0 62.5 64.0 65.6 67.1 68.7 70.3

34 36.5 37.7 39.0 40.3 41.5 42.8 44.1 45.4 46.8 48.1 49.5 50.8 52.2 53.6 55.0 56.5 57.9 59.4 60.9 62.3 63.9 65.4 66.9 68.5 70.1 71.7

35 37.6 38.9 40.2 41.4 42.7 44.0 45.3 46.7 48.0 49.3 50.7 52.1 53.5 54.9 56.3 57.8 59.2 60.7 62.2 63.7 65.2 66.8 68.3 69.9 71.5 73.1

36 38.8 40.1 41.3 42.6 43.9 45.2 46.5 47.9 49.2 50.6 52.0 53.4 54.8 56.2 57.6 59.1 60.5 62.0 63.5 65.0 66.6 68.1 69.7 71.3 72.9 74.5

37 40.0 41.2 42.5 43.8 45.1 46.4 47.8 49.1 50.5 51.8 53.2 54.6 56.0 57.5 58.9 60.4 61.9 63.4 64.9 66.4 67.9 69.5 71.1 72.7 74.3 76.0

38 41.1 42.4 43.7 45.0 46.3 47.7 49.0 50.3 51.7 53.1 54.5 55.9 57.3 58.8 60.2 61.7 63.2 64.7 66.2 67.8 69.3 70.9 72.5 74.1 75.8 77.4

39 42.3 43.6 44.9 46.2 47.5 48.9 50.2 51.6 53.0 54.4 55.8 57.2 58.6 60.1 61.5 63.0 64.5 66.1 67.6 69.1 70.7 72.3 73.9 75.5 77.2 78.9

40 43.5 44.8 46.1 47.4 48.8 50.1 51.5 52.8 54.2 55.6 57.0 58.5 59.9 61.4 62.9 64.4 65.9 67.4 69.0 70.5 72.1 73.7 75.3 77.0 78.6 80.3

41 44.7 46.0 47.3 48.6 50.0 51.3 52.7 54.1 55.5 56.9 58.3 59.8 61.2 62.7 64.2 65.7 67.2 68.8 70.3 71.9 73.5 75.1 76.8 78.4 80.1 81.8

42 45.9 47.2 48.5 49.9 51.2 52.6 54.0 55.4 56.8 58.2 59.6 61.1 62.5 64.0 65.5 67.1 68.6 70.1 71.7 73.3 74.9 76.6 78.2 79.9 81.6 83.3

43 47.1 48.4 49.7 51.1 52.5 53.8 55.2 56.6 58.0 59.5 60.9 62.4 63.9 65.4 66.9 68.4 70.0 71.5 73.1 74.7 76.3 78.0 79.6 81.3 83.0 84.8

44 48.3 49.6 51.0 52.3 53.7 55.1 56.5 57.9 59.3 60.8 62.2 63.7 65.2 66.7 68.2 69.8 71.3 72.9 74.5 76.1 77.8 79.4 81.1 82.8 84.5 86.3

45 49.5 50.8 52.2 53.6 54.9 56.3 57.7 59.2 60.6 62.1 63.5 65.0 66.5 68.0 69.6 71.1 72.7 74.3 75.9 77.5 79.2 80.9 82.6 84.3 86.0 87.8

46 50.7 52.1 53.4 54.8 56.2 57.6 59.0 60.5 61.9 63.4 64.9 66.4 67.9 69.4 71.0 72.5 74.1 75.7 77.3 79.0 80.6 82.3 84.0 85.8 87.5 89.3

47 51.9 53.3 54.7 56.1 57.5 58.9 60.3 61.8 63.2 64.7 66.2 67.7 69.2 70.8 72.3 73.9 75.5 77.1 78.8 80.4 82.1 83.8 85.5 87.3 89.0 90.8

48 53.2 54.5 55.9 57.3 58.7 60.2 61.6 63.1 64.5 66.0 67.5 69.0 70.6 72.1 73.7 75.3 76.9 78.5 80.2 81.9 83.6 85.3 87.0 88.8 90.5 92.4

49 54.4 55.8 57.2 58.6 60.0 61.4 62.9 64.4 65.8 67.3 68.9 70.4 71.9 73.5 75.1 76.7 78.3 80.0 81.6 83.3 85.0 86.8 88.5 90.3 92.1 93.9

50 55.7 57.0 58.4 59.9 61.3 62.7 64.2 65.7 67.2 68.7 70.2 71.7 73.3 74.9 76.5 78.1 79.7 81.4 83.1 84.8 86.5 88.2 90.0 91.8 93.6 95.5

51 56.9 58.3 59.7 61.1 62.6 64.0 65.5 67.0 68.5 70.0 71.6 73.1 74.7 76.3 77.9 79.5 81.2 82.9 84.5 86.3 88.0 89.8 91.5 93.3 95.2 97.0
 Number of positive wells—both undiluted trays
26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51

0 29.1 30.3 31.6 32.9 34.2 35.5 36.8 38.2 39.5 40.9 42.3 43.7 45.1 46.6 48.0 49.5 50.9 52.4 54.0 55.5 57.1 58.6 60.2 61.8 63.5 65.1

1 30.3 31.5 32.8 34.1 35.4 36.8 38.1 39.4 40.8 42.2 43.6 45.0 46.4 47.9 49.3 50.8 52.3 53.8 55.4 56.9 58.5 60.1 61.7 63.3 64.9 66.6

2 31.5 32.7 34.0 35.3 36.7 38.0 39.3 40.7 42.1 43.5 44.9 46.3 47.8 49.2 50.7 52.2 53.7 55.2 56.7 58.3 59.9 61.5 63.1 64.8 66.4 68.1

3 32.7 34.0 35.3 36.6 37.9 39.3 40.6 42.0 43.4 44.8 46.2 47.6 49.1 50.5 52.0 53.5 55.1 56.6 58.1 59.7 61.3 62.9 64.6 66.2 67.9 69.6

4 33.9 35.2 36.5 37.8 39.2 40.5 41.9 43.3 44.7 46.1 47.5 49.0 50.4 51.9 53.4 54.9 56.4 58.0 59.6 61.1 62.8 64.4 66.0 67.7 69.4 71.1

5 35.1 36.4 37.7 39.1 40.4 41.8 43.2 44.5 46.0 47.4 48.8 50.3 51.8 53.2 54.8 56.3 57.8 59.4 61.0 62.6 64.2 65.9 67.5 69.2 70.9 72.6

6 36.3 37.6 39.0 40.3 41.7 43.0 44.4 45.8 47.3 48.7 50.2 51.6 53.1 54.6 56.1 57.7 59.2 60.8 62.4 64.0 65.7 67.3 69.0 70.7 72.4 74.2

7 37.5 38.9 40.2 41.6 42.9 44.3 45.7 47.1 48.6 50.0 51.5 53.0 54.5 56.0 57.5 59.1 60.6 62.2 63.8 65.5 67.1 68.8 70.5 72.2 74.0 75.7

8 38.8 40.1 41.5 42.8 44.2 45.6 47.0 48.5 49.9 51.4 52.8 54.3 55.8 57.4 58.9 60.5 62.1 63.7 65.3 66.9 68.6 70.3 72.0 73.7 75.5 77.3

9 40.0 41.4 42.7 44.1 45.5 46.9 48.3 49.8 51.2 52.7 54.2 55.7 57.2 58.7 60.3 61.9 63.5 65.1 66.7 68.4 70.1 71.8 73.5 75.3 77.0 78.8

10 41.3 42.6 44.0 45.4 46.8 48.2 49.6 51.1 52.6 54.0 55.5 57.1 58.6 60.1 61.7 63.3 64.9 66.6 68.2 69.9 71.6 73.3 75.0 76.8 78.6 80.4

11 42.5 43.9 45.3 46.7 48.1 49.5 51.0 52.4 53.9 55.4 56.9 58.4 60.0 61.5 63.1 64.7 66.4 68.0 69.7 71.4 73.1 74.8 76.6 78.4 80.2 82.0

12 43.8 45.2 46.6 48.0 49.4 50.8 52.3 53.8 55.2 56.7 58.3 59.8 61.4 63.0 64.6 66.2 67.8 69.5 71.2 72.9 74.6 76.4 78.1 79.9 81.8 83.6

13 45.0 46.4 47.8 49.3 50.7 52.1 53.6 55.1 56.6 58.1 59.7 61.2 62.8 64.4 66.0 67.6 69.3 71.0 72.7 74.4 76.1 77.9 79.7 81.5 83.4 85.2

14 46.3 47.7 49.1 50.6 52.0 53.5 55.0 56.4 58.0 59.5 61.0 62.6 64.2 65.8 67.4 69.1 70.8 72.5 74.2 75.9 77.7 79.4 81.3 83.1 85.0 86.8

15 47.6 49.0 50.4 51.9 53.3 54.8 56.3 57.8 59.3 60.9 62.4 64.0 65.6 67.3 68.9 70.6 72.2 73.9 75.7 77.4 79.2 81.0 82.8 84.7 86.6 88.5

16 48.9 50.3 51.7 53.2 54.7 56.2 57.7 59.2 60.7 62.3 63.8 65.4 67.1 68.7 70.4 72.0 73.7 75.5 77.2 79.0 80.8 82.6 84.4 86.3 88.2 90.1

17 50.2 51.6 53.1 54.5 56.0 57.5 59.0 60.6 62.1 63.7 65.3 66.9 68.5 70.2 71.8 73.5 75.2 77.0 78.7 80.5 82.3 84.2 86.0 87.9 89.8 91.8
Number of positive wells—diluted tray

18 51.5 52.9 54.4 55.9 57.4 58.9 60.4 61.9 63.5 65.1 66.7 68.3 70.0 71.6 73.3 75.0 76.7 78.5 80.3 82.1 83.9 85.8 87.6 89.6 91.5 93.5

19 52.8 54.2 55.7 57.2 58.7 60.2 61.8 63.3 64.9 66.5 68.1 69.8 71.4 73.1 74.8 76.5 78.3 80.0 81.8 83.7 85.5 87.4 89.3 91.2 93.2 95.1

20 54.1 55.6 57.1 58.6 60.1 61.6 63.2 64.7 66.3 67.9 69.6 71.2 72.9 74.6 76.3 78.0 79.8 81.6 83.4 85.2 87.1 89.0 90.9 92.9 94.8 96.8

21 55.4 56.9 58.4 59.9 61.4 63.0 64.6 66.1 67.7 69.4 71.0 72.7 74.4 76.1 77.8 79.6 81.3 83.1 85.0 86.8 88.7 90.6 92.6 94.5 96.5 98.5

22 56.8 58.2 59.7 61.3 62.8 64.4 66.0 67.6 69.2 70.8 72.5 74.1 75.9 77.6 79.3 81.1 82.9 84.7 86.6 88.4 90.3 92.3 94.2 96.2 98.2 100.3

23 58.1 59.6 61.1 62.6 64.2 65.8 67.4 69.0 70.6 72.3 73.9 75.6 77.3 79.1 80.9 82.6 84.5 86.3 88.2 90.0 92.0 93.9 95.9 97.9 99.9 102.0

24 59.4 60.9 62.5 64.0 65.6 67.2 68.8 70.4 72.1 73.7 75.4 77.1 78.9 80.6 82.4 84.2 86.0 87.9 89.8 91.7 93.6 95.6 97.6 99.6 101.7 103.7

25 60.8 62.3 63.9 65.4 67.0 68.6 70.2 71.9 73.5 75.2 76.9 78.6 80.4 82.1 83.9 85.8 87.6 89.5 91.4 93.3 95.3 97.3 99.3 101.3 103.4 105.5

26 62.1 63.7 65.2 66.8 68.4 70.0 71.7 73.3 75.0 76.7 78.4 80.1 81.9 83.7 85.5 87.3 89.2 91.1 93.0 95.0 96.9 98.9 101.0 103.0 105.1 107.3

27 63.5 65.1 66.6 68.2 69.8 71.5 73.1 74.8 76.5 78.2 79.9 81.7 83.4 85.2 87.1 88.9 90.8 92.7 94.7 96.6 98.6 100.6 102.7 104.8 106.9 109.1

28 64.9 66.5 68.0 69.6 71.3 72.9 74.6 76.2 77.9 79.7 81.4 83.2 85.0 86.8 88.7 90.5 92.4 94.4 96.3 98.3 100.3 102.4 104.4 106.5 108.7 110.9

29 66.3 67.8 69.4 71.1 72.7 74.3 76.0 77.7 79.4 81.2 82.9 84.7 86.5 88.4 90.2 92.1 94.1 96.0 98.0 100.0 102.0 104.1 106.2 108.3 110.5 112.7

30 67.7 69.3 70.9 72.5 74.1 75.8 77.5 79.2 80.9 82.7 84.5 86.3 88.1 90.0 91.8 93.8 95.7 97.7 99.7 101.7 103.7 105.8 108.0 110.1 112.3 114.5

31 69.1 70.7 72.3 73.9 75.6 77.3 79.0 80.7 82.5 84.2 86.0 87.8 89.7 91.6 93.5 95.4 97.3 99.3 101.3 103.4 105.5 107.6 109.7 111.9 114.1 116.4

32 70.5 72.1 73.7 75.4 77.0 78.7 80.5 82.2 84.0 85.8 87.6 89.4 91.3 93.2 95.1 97.0 99.0 101.0 103.1 105.1 107.2 109.4 111.5 113.7 116.0 118.2

33 71.9 73.5 75.2 76.8 78.5 80.2 82.0 83.7 85.5 87.3 89.1 91.0 92.9 94.8 96.7 98.7 100.7 102.7 104.8 106.9 109.0 111.1 113.3 115.6 117.8 120.1

34 73.3 75.0 76.6 78.3 80.0 81.7 83.5 85.3 87.1 88.9 90.7 92.6 94.5 96.4 98.4 100.4 102.4 104.4 106.5 108.6 110.8 112.9 115.1 117.4 119.7 122.0

35 74.7 76.4 78.1 79.8 81.5 83.2 85.0 86.8 88.6 90.4 92.3 94.2 96.1 98.1 100.0 102.0 104.1 106.1 108.2 110.4 112.5 114.7 117.0 119.3 121.6 123.9

36 76.2 77.9 79.5 81.3 83.0 84.7 86.5 88.3 90.2 92.0 93.9 95.8 97.7 99.7 101.7 103.7 105.8 107.9 110.0 112.2 114.3 116.6 118.8 121.1 123.5 125.9

37 77.6 79.3 81.0 82.7 84.5 86.3 88.1 89.9 91.7 93.6 95.5 97.4 99.4 101.4 103.4 105.4 107.5 109.6 111.8 113.9 116.2 118.4 120.7 123.0 125.4 127.8

38 79.1 80.8 82.5 84.3 86.0 87.8 89.6 91.5 93.3 95.2 97.1 99.1 101.1 103.1 105.1 107.2 109.3 111.4 113.5 115.8 118.0 120.3 122.6 124.9 127.3 129.8

39 80.6 82.3 84.0 85.8 87.5 89.4 91.2 93.0 94.9 96.8 98.8 100.7 102.7 104.7 106.8 108.9 111.0 113.2 115.3 117.6 119.8 122.1 124.5 126.9 129.3 131.7

40 82.0 83.8 85.5 87.3 89.1 90.9 92.8 94.6 96.5 98.5 100.4 102.4 104.4 106.4 108.5 110.6 112.8 114.9 117.2 119.4 121.7 124.0 126.4 128.8 131.2 133.7

41 83.5 85.3 87.0 88.8 90.6 92.5 94.3 96.2 98.1 100.1 102.1 104.1 106.1 108.2 110.3 112.4 114.6 116.8 119.0 121.3 123.6 125.9 128.3 130.8 133.2 135.8

42 85.0 86.8 88.6 90.4 92.2 94.0 95.9 97.8 99.8 101.7 103.7 105.8 107.8 109.9 112.0 114.2 116.3 118.6 120.8 123.1 125.5 127.8 130.3 132.7 135.2 137.8

43 86.5 88.3 90.1 91.9 93.8 95.6 97.5 99.5 101.4 103.4 105.4 107.4 109.5 111.6 113.8 115.9 118.2 120.4 122.7 125.0 127.4 129.8 132.2 134.7 137.3 139.8

44 88.0 89.8 91.6 93.5 95.3 97.2 99.1 101.1 103.1 105.1 107.1 109.2 111.3 113.4 115.5 117.7 120.0 122.2 124.6 126.9 129.3 131.7 134.2 136.7 139.3 141.9

45 89.6 91.4 93.2 95.0 96.9 98.8 100.8 102.7 104.7 106.7 108.8 110.9 113.0 115.1 117.3 119.6 121.8 124.1 126.4 128.8 131.2 133.7 136.2 138.8 141.4 144.0

46 91.1 92.9 94.8 96.6 98.5 100.5 102.4 104.4 106.4 108.4 110.5 112.6 114.8 116.9 119.1 121.4 123.7 126.0 128.3 130.7 133.2 135.7 138.2 140.8 143.4 146.1

47 92.6 94.5 96.3 98.2 100.1 102.1 104.1 106.1 108.1 110.1 112.2 114.4 116.5 118.7 121.0 123.2 125.5 127.9 130.3 132.7 135.2 137.7 140.2 142.9 145.5 148.2

48 94.2 96.0 97.9 99.8 101.8 103.7 105.7 107.7 109.8 111.9 114.0 116.1 118.3 120.5 122.8 125.1 127.4 129.8 132.2 134.7 137.2 139.7 142.3 144.9 147.6 150.4

49 95.7 97.6 99.5 101.4 103.4 105.4 107.4 109.4 111.5 113.6 115.7 117.9 120.1 122.4 124.6 126.9 129.3 131.7 134.1 136.6 139.2 141.7 144.4 147.1 149.8 152.6

50 97.3 99.2 101.1 103.1 105.0 107.0 109.1 111.1 113.2 115.3 117.5 119.7 121.9 124.2 126.5 128.8 131.2 133.6 136.1 138.6 141.2 143.8 146.5 149.2 151.9 154.8

51 98.9 100.8 102.7 104.7 106.7 108.7 110.8 112.8 115.0 117.1 119.3 121.5 123.8 126.0 128.4 130.7 133.1 135.6 138.1 140.6 143.2 145.9 148.6 151.3 154.1 157.0
 Number of positive wells—both undiluted trays
52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77

0 66.8 68.5 70.2 72.0 73.7 75.5 77.4 79.2 81.1 83.0 84.9 86.9 88.9 90.9 93.0 95.0 97.2 99.3 101.5 103.8 106.0 108.4 110.7 113.1 115.6 118.1

1 68.3 70.0 71.8 73.5 75.3 77.1 79.0 80.8 82.7 84.6 86.6 88.6 90.6 92.6 94.7 96.8 99.0 101.2 103.4 105.7 108.0 110.3 112.7 115.1 117.6 120.2

2 69.8 71.5 73.3 75.1 76.9 78.7 80.6 82.5 84.4 86.3 88.3 90.3 92.3 94.4 96.5 98.6 100.8 103.0 105.3 107.6 109.9 112.3 114.7 117.2 119.7 122.2

3 71.3 73.1 74.9 76.7 78.5 80.3 82.2 84.1 86.0 88.0 90.0 92.0 94.1 96.2 98.3 100.5 102.7 104.9 107.2 109.5 111.8 114.2 116.7 119.2 121.7 124.3

4 72.9 74.6 76.4 78.2 80.1 81.9 83.8 85.8 87.7 89.7 91.7 93.8 95.8 98.0 100.1 102.3 104.5 106.8 109.1 111.4 113.8 116.2 118.7 121.3 123.8 126.5

5 74.4 76.2 78.0 79.8 81.7 83.6 85.5 87.4 89.4 91.4 93.4 95.5 97.6 99.8 101.9 104.1 106.4 108.7 111.0 113.4 115.8 118.3 120.8 123.3 126.0 128.6

6 76.0 77.8 79.6 81.4 83.3 85.2 87.1 89.1 91.1 93.1 95.2 97.3 99.4 101.6 103.8 106.0 108.3 110.6 113.0 115.4 117.8 120.3 122.8 125.4 128.1 130.8

7 77.5 79.3 81.2 83.0 84.9 86.9 88.8 90.8 92.8 94.9 96.9 99.1 101.2 103.4 105.6 107.9 110.2 112.5 114.9 117.4 119.8 122.4 124.9 127.6 130.3 133.0

8 79.1 80.9 82.8 84.7 86.6 88.5 90.5 92.5 94.5 96.6 98.7 100.9 103.0 105.2 107.5 109.8 112.1 114.5 116.9 119.4 121.9 124.4 127.1 129.7 132.4 135.2

9 80.7 82.5 84.4 86.3 88.2 90.2 92.2 94.2 96.3 98.4 100.5 102.7 104.9 107.1 109.4 111.7 114.1 116.5 118.9 121.4 123.9 126.5 129.2 131.9 134.7 137.5

10 82.3 84.1 86.0 88.0 89.9 91.9 93.9 96.0 98.0 100.2 102.3 104.5 106.7 109.0 111.3 113.6 116.0 118.4 120.9 123.4 126.0 128.7 131.3 134.1 136.9 139.7

11 83.9 85.8 87.7 89.6 91.6 93.6 95.6 97.7 99.8 101.9 104.1 106.3 108.6 110.9 113.2 115.6 118.0 120.5 123.0 125.5 128.1 130.8 133.5 136.3 139.1 142.0

12 85.5 87.4 89.3 91.3 93.3 95.3 97.4 99.5 101.6 103.8 106.0 108.2 110.5 112.8 115.1 117.5 120.0 122.5 125.0 127.6 130.3 133.0 135.7 138.5 141.4 144.4

13 87.1 89.0 91.0 93.0 95.0 97.0 99.1 101.2 103.4 105.6 107.8 110.1 112.4 114.7 117.1 119.5 122.0 124.5 127.1 129.7 132.4 135.1 137.9 140.8 143.7 146.7

14 88.8 90.7 92.7 94.7 96.7 98.8 100.9 103.0 105.2 107.4 109.7 112.0 114.3 116.7 119.1 121.5 124.0 126.6 129.2 131.9 134.6 137.4 140.2 143.1 146.1 149.1

15 90.4 92.4 94.4 96.4 98.5 100.6 102.7 104.8 107.0 109.3 111.5 113.9 116.2 118.6 121.1 123.6 126.1 128.7 131.3 134.0 136.8 139.6 142.5 145.4 148.4 151.5

16 92.1 94.1 96.1 98.1 100.2 102.3 104.5 106.7 108.9 111.1 113.4 115.8 118.2 120.6 123.1 125.6 128.2 130.8 133.5 136.2 139.0 141.8 144.8 147.7 150.8 153.9

17 93.8 95.8 97.8 99.9 102.0 104.1 106.3 108.5 110.7 113.0 115.4 117.7 120.1 122.6 125.1 127.7 130.3 132.9 135.6 138.4 141.2 144.1 147.1 150.1 153.2 156.4
Number of positive wells—diluted tray

18 95.4 97.5 99.5 101.6 103.8 105.9 108.1 110.3 112.6 114.9 117.3 119.7 122.1 124.6 127.1 129.7 132.4 135.1 137.8 140.6 143.5 146.4 149.4 152.5 155.7 158.9

19 97.2 99.2 101.3 103.4 105.5 107.7 109.9 112.2 114.5 116.8 119.2 121.7 124.1 126.6 129.2 131.8 134.5 137.2 140.0 142.9 145.8 148.8 151.8 154.9 158.1 161.4

20 98.9 100.9 103.0 105.2 107.3 109.6 111.8 114.1 116.4 118.8 121.2 123.7 126.2 128.7 131.3 134.0 136.7 139.4 142.3 145.2 148.1 151.1 154.2 157.4 160.6 163.9

21 100.6 102.7 104.8 107.0 109.2 111.4 113.7 116.0 118.3 120.7 123.2 125.7 128.2 130.8 133.4 136.1 138.9 141.7 144.5 147.5 150.4 153.5 156.6 159.9 163.2 166.5

22 102.3 104.5 106.6 108.8 111.0 113.3 115.6 117.9 120.3 122.7 125.2 127.7 130.3 132.9 135.5 138.3 141.1 143.9 146.8 149.8 152.8 155.9 159.1 162.4 165.7 169.1

23 104.1 106.2 108.4 110.6 112.9 115.1 117.5 119.8 122.2 124.7 127.2 129.7 132.3 135.0 137.7 140.5 143.3 146.2 149.1 152.1 155.2 158.4 161.6 164.9 168.3 171.8

24 105.9 108.0 110.2 112.5 114.7 117.0 119.4 121.8 124.2 126.7 129.2 131.8 134.4 137.1 139.9 142.7 145.5 148.5 151.4 154.5 157.6 160.8 164.1 167.5 170.9 174.5

25 107.7 109.8 112.1 114.3 116.6 118.9 121.3 123.7 126.2 128.7 131.3 133.9 136.6 139.3 142.1 144.9 147.8 150.8 153.8 156.9 160.1 163.3 166.7 170.1 173.6 177.2

26 109.5 111.7 113.9 116.2 118.5 120.9 123.3 125.7 128.2 130.8 133.4 136.0 138.7 141.5 144.3 147.2 150.1 153.1 156.2 159.3 162.6 165.9 169.3 172.7 176.3 180.0

27 111.3 113.5 115.8 118.1 120.4 122.8 125.3 127.7 130.3 132.8 135.5 138.1 140.9 143.7 146.5 149.4 152.4 155.5 158.6 161.8 165.1 168.4 171.9 175.4 179.0 182.8

28 113.1 115.4 117.6 120.0 122.4 124.8 127.2 129.8 132.3 134.9 137.6 140.3 143.1 145.9 148.8 151.8 154.8 157.9 161.0 164.3 167.6 171.0 174.5 178.1 181.8 185.6

29 114.9 117.2 119.5 121.9 124.3 126.8 129.2 131.8 134.4 137.0 139.7 142.5 145.3 148.2 151.1 154.1 157.2 160.3 163.5 166.8 170.2 173.6 177.2 180.9 184.6 188.5

30 116.8 119.1 121.5 123.8 126.3 128.8 131.3 133.9 136.5 139.2 141.9 144.7 147.5 150.4 153.4 156.4 159.6 162.7 166.0 169.4 172.8 176.3 179.9 183.6 187.5 191.4

31 118.7 121.0 123.4 125.8 128.3 130.8 133.3 135.9 138.6 141.3 144.1 146.9 149.8 152.7 155.7 158.8 162.0 165.2 168.5 171.9 175.4 179.0 182.7 186.5 190.3 194.3

32 120.6 122.9 125.3 127.8 130.3 132.8 135.4 138.0 140.7 143.5 146.3 149.1 152.1 155.1 158.1 161.2 164.4 167.7 171.1 174.5 178.1 181.7 185.5 189.3 193.3 197.3

33 122.5 124.9 127.3 129.8 132.3 134.9 137.5 140.1 142.9 145.7 148.5 151.4 154.4 157.4 160.5 163.7 166.9 170.3 173.7 177.2 180.8 184.5 188.3 192.2 196.2 200.4

34 124.4 126.8 129.3 131.8 134.3 136.9 139.6 142.3 145.0 147.9 150.7 153.7 156.7 159.8 162.9 166.1 169.5 172.8 176.3 179.9 183.5 187.3 191.2 195.2 199.3 203.5

35 126.3 128.8 131.3 133.8 136.4 139.0 141.7 144.4 147.2 150.1 153.0 156.0 159.1 162.2 165.4 168.6 172.0 175.4 179.0 182.6 186.3 190.1 194.1 198.1 202.3 206.6

36 128.3 130.8 133.3 135.8 138.5 141.1 143.8 146.6 149.5 152.4 155.3 158.3 161.4 164.6 167.8 171.2 174.6 178.1 181.7 185.3 189.1 193.0 197.0 201.2 205.4 209.8

37 130.3 132.8 135.3 137.9 140.6 143.3 146.0 148.8 151.7 154.6 157.6 160.7 163.8 167.1 170.4 173.7 177.2 180.7 184.4 188.1 192.0 195.9 200.0 204.2 208.6 213.1

38 132.3 134.8 137.4 140.0 142.7 145.4 148.2 151.1 154.0 156.9 160.0 163.1 166.3 169.5 172.9 176.3 179.8 183.4 187.1 191.0 194.9 198.9 203.1 207.4 211.8 216.3

39 134.3 136.8 139.4 142.1 144.8 147.6 150.4 153.3 156.3 159.3 162.4 165.5 168.8 172.1 175.5 178.9 182.5 186.2 189.9 193.8 197.8 201.9 206.1 210.5 215.0 219.7

40 136.3 138.9 141.5 144.2 147.0 149.8 152.6 155.6 158.6 161.6 164.8 168.0 171.2 174.6 178.1 181.6 185.2 188.9 192.8 196.7 200.8 205.0 209.3 213.7 218.3 223.1

41 138.3 141.0 143.6 146.4 149.2 152.0 154.9 157.9 160.9 164.0 167.2 170.4 173.8 177.2 180.7 184.3 188.0 191.8 195.7 199.7 203.8 208.1 212.5 217.0 221.7 226.6

42 140.4 143.1 145.8 148.5 151.4 154.2 157.2 160.2 163.3 166.4 169.6 172.9 176.3 179.8 183.3 187.0 190.8 194.6 198.6 202.7 206.9 211.2 215.7 220.3 225.1 230.1

43 142.5 145.2 147.9 150.7 153.6 156.5 159.5 162.5 165.7 168.9 172.1 175.5 178.9 182.4 186.0 189.8 193.6 197.5 201.5 205.7 210.0 214.4 219.0 223.7 228.6 233.7

44 144.6 147.3 150.1 152.9 155.8 158.8 161.8 164.9 168.1 171.3 174.6 178.0 181.5 185.1 188.8 192.5 196.4 200.4 204.5 208.8 213.1 217.6 222.3 227.1 232.1 237.3

45 146.7 149.5 152.3 155.2 158.1 161.1 164.2 167.3 170.5 173.8 177.2 180.6 184.2 187.8 191.5 195.4 199.3 203.4 207.6 211.9 216.3 220.9 225.7 230.6 235.7 241.0

46 148.9 151.7 154.5 157.4 160.4 163.4 166.5 169.7 173.0 176.3 179.7 183.3 186.9 190.6 194.3 198.2 202.3 206.4 210.7 215.1 219.6 224.3 229.1 234.2 239.4 244.8

47 151.0 153.9 156.7 159.7 162.7 165.8 169.0 172.2 175.5 178.9 182.4 185.9 189.6 193.3 197.2 201.2 205.2 209.5 213.8 218.3 222.9 227.7 232.6 237.8 243.1 248.6

48 153.2 156.1 159.0 162.0 165.1 168.2 171.4 174.7 178.0 181.5 185.0 188.6 192.3 196.1 200.1 204.1 208.3 212.6 217.0 221.5 226.3 231.1 236.2 241.5 246.9 252.6

49 155.4 158.3 161.3 164.3 167.4 170.6 173.8 177.2 180.6 184.1 187.7 191.3 195.1 199.0 203.0 207.1 211.3 215.7 220.2 224.9 229.7 234.7 239.8 245.2 250.8 256.6

50 157.6 160.6 163.6 166.7 169.8 173.0 176.3 179.7 183.2 186.7 190.4 194.1 197.9 201.9 205.9 210.1 214.4 218.9 223.5 228.2 233.2 238.2 243.5 249.0 254.7 260.7

51 159.9 162.9 165.9 169.1 172.2 175.5 178.9 182.3 185.8 189.4 193.1 196.9 200.8 204.8 208.9 213.2 217.6 222.1 226.8 231.7 236.7 241.9 247.3 252.9 258.7 264.8
 Number of positive wells—both undiluted trays
78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102

0 120.6 123.2 125.9 128.6 131.4 134.2 137.1 140.1 143.1 146.2 149.4 152.7 156.0 159.5 163.0 166.7 170.4 174.3 178.3 182.4 186.6 191.0 195.6 200.3 205.2

1 122.7 125.4 128.1 130.8 133.6 136.5 139.4 142.5 145.5 148.7 151.9 155.3 158.7 162.2 165.8 169.5 173.3 177.3 181.3 185.5 189.9 194.3 199.0 203.8 208.9

2 124.9 127.5 130.3 133.1 135.9 138.8 141.8 144.9 148.0 151.2 154.5 157.9 161.3 164.9 168.6 172.4 176.3 180.3 184.4 188.7 193.1 197.7 202.5 207.4 212.6

3 127.0 129.7 132.5 135.3 138.2 141.2 144.2 147.3 150.5 153.7 157.1 160.5 164.1 167.7 171.4 175.3 179.2 183.3 187.6 191.9 196.5 201.2 206.0 211.1 216.4

4 129.2 131.9 134.7 137.6 140.5 143.5 146.6 149.8 153.0 156.3 159.7 163.2 166.8 170.5 174.3 178.2 182.3 186.5 190.8 195.3 199.9 204.7 209.7 214.9 220.3

5 131.4 134.1 137.0 139.9 142.9 145.9 149.1 152.3 155.6 158.9 162.4 165.9 169.6 173.4 177.2 181.2 185.4 189.6 194.0 198.6 203.3 208.3 213.4 218.7 224.2

6 133.6 136.4 139.3 142.2 145.3 148.4 151.5 154.8 158.1 161.6 165.1 168.7 172.4 176.3 180.2 184.3 188.5 192.9 197.4 202.0 206.9 211.9 217.1 222.6 228.3

7 135.8 138.7 141.6 144.6 147.7 150.8 154.1 157.4 160.8 164.2 167.8 171.5 175.3 179.2 183.2 187.4 191.7 196.1 200.7 205.5 210.5 215.6 221.0 226.6 232.5

8 138.1 141.0 144.0 147.0 150.1 153.3 156.6 160.0 163.4 167.0 170.6 174.4 178.2 182.2 186.3 190.6 194.9 199.5 204.2 209.1 214.1 219.4 224.9 230.7 236.7

9 140.4 143.3 146.3 149.4 152.6 155.8 159.2 162.6 166.1 169.7 173.4 177.2 181.2 185.2 189.4 193.8 198.2 202.9 207.7 212.7 217.9 223.3 229.0 234.9 241.0

10 142.7 145.7 148.7 151.9 155.1 158.4 161.8 165.2 168.8 172.5 176.3 180.2 184.2 188.3 192.6 197.0 201.6 206.3 211.3 216.4 221.7 227.3 233.1 239.1 245.5

11 145.0 148.1 151.2 154.4 157.6 161.0 164.4 167.9 171.6 175.3 179.2 183.1 187.2 191.5 195.8 200.3 205.0 209.9 214.9 220.1 225.6 231.3 237.3 243.5 250.1

12 147.4 150.5 153.6 156.9 160.2 163.6 167.1 170.7 174.4 178.2 182.1 186.2 190.3 194.6 199.1 203.7 208.5 213.5 218.6 224.0 229.6 235.4 241.6 248.0 254.7

13 149.8 152.9 156.1 159.4 162.8 166.2 169.8 173.5 177.2 181.1 185.1 189.2 193.5 197.9 202.4 207.1 212.0 217.1 222.4 227.9 233.7 239.7 246.0 252.6 259.5

14 152.2 155.4 158.6 162.0 165.4 168.9 172.6 176.3 180.1 184.1 188.1 192.3 196.7 201.2 205.8 210.6 215.6 220.8 226.3 231.9 237.8 244.0 250.5 257.3 264.5

15 154.6 157.9 161.2 164.6 168.1 171.7 175.3 179.1 183.0 187.1 191.2 195.5 199.9 204.5 209.3 214.2 219.3 224.7 230.2 236.0 242.1 248.4 255.1 262.1 269.6

16 157.1 160.4 163.8 167.2 170.8 174.4 178.2 182.0 186.0 190.1 194.3 198.7 203.2 207.9 212.8 217.8 223.1 228.5 234.2 240.2 246.4 253.0 259.8 267.1 274.8

17 159.6 163.0 166.4 169.9 173.5 177.2 181.0 185.0 189.0 193.2 197.5 202.0 206.6 211.4 216.4 221.5 226.9 232.5 238.4 244.5 250.9 257.6 264.7 272.2 280.1
Number of positive wells—diluted tray

18 162.2 165.6 169.0 172.6 176.3 180.1 184.0 188.0 192.1 196.4 200.8 205.3 210.0 214.9 220.0 225.3 230.8 236.6 242.6 248.8 255.4 262.4 269.7 277.5 285.7

19 164.8 168.2 171.7 175.4 179.1 182.9 186.9 191.0 195.2 199.5 204.0 208.7 213.5 218.5 223.7 229.2 234.8 240.7 246.9 253.3 260.1 267.3 274.9 282.9 291.4

20 167.4 170.9 174.5 178.2 182.0 185.9 189.9 194.1 198.4 202.8 207.4 212.1 217.1 222.2 227.5 233.1 238.9 244.9 251.3 257.9 264.9 272.3 280.1 288.4 297.3

21 170.0 173.6 177.2 181.0 184.8 188.8 192.9 197.2 201.6 206.1 210.8 215.7 220.7 225.9 231.4 237.1 243.0 249.3 255.8 262.6 269.9 277.5 285.6 294.2 303.4

22 172.7 176.3 180.0 183.8 187.8 191.8 196.0 200.4 204.8 209.5 214.3 219.2 224.4 229.8 235.4 241.2 247.3 253.7 260.4 267.5 274.9 282.8 291.2 300.1 309.7

23 175.4 179.1 182.9 186.8 190.8 194.9 199.2 203.6 208.2 212.9 217.8 222.9 228.2 233.7 239.4 245.4 251.7 258.2 265.2 272.4 280.1 288.3 297.0 306.3 316.3

24 178.1 181.9 185.7 189.7 193.8 198.0 202.4 206.9 211.5 216.4 221.4 226.6 232.0 237.6 243.5 249.7 256.1 262.9 270.0 277.5 285.5 294.0 303.0 312.7 323.0

25 180.9 184.7 188.7 192.7 196.9 201.2 205.6 210.2 215.0 219.9 225.0 230.4 235.9 241.7 247.7 254.0 260.7 267.7 275.0 282.8 291.0 299.8 309.2 319.2 330.1

26 183.7 187.6 191.6 195.7 200.0 204.4 208.9 213.6 218.5 223.5 228.8 234.2 239.9 245.8 252.0 258.5 265.4 272.6 280.1 288.2 296.7 305.8 315.6 326.1 337.4

27 186.6 190.6 194.6 198.8 203.2 207.6 212.3 217.1 222.0 227.2 232.6 238.1 244.0 250.1 256.4 263.1 270.2 277.6 285.4 293.7 302.6 312.1 322.2 333.2 345.1

28 189.5 193.5 197.7 202.0 206.4 210.9 215.7 220.6 225.7 230.9 236.4 242.2 248.1 254.4 261.0 267.8 275.1 282.7 290.9 299.5 308.7 318.5 329.1 340.6 353.1

29 192.4 196.5 200.8 205.1 209.6 214.3 219.1 224.1 229.3 234.8 240.4 246.3 252.4 258.8 265.6 272.7 280.1 288.1 296.5 305.4 314.9 325.2 336.3 348.3 361.5

30 195.4 199.6 203.9 208.4 213.0 217.7 222.7 227.8 233.1 238.6 244.4 250.4 256.8 263.4 270.3 277.6 285.3 293.5 302.2 311.5 321.4 332.1 343.7 356.4 370.2

31 198.5 202.7 207.1 211.7 216.4 221.2 226.3 231.5 236.9 242.6 248.5 254.7 261.2 268.0 275.2 282.7 290.7 299.2 308.2 317.8 328.2 339.4 351.5 364.8 379.5

32 201.5 205.9 210.4 215.0 219.8 224.8 229.9 235.3 240.9 246.7 252.8 259.1 265.8 272.8 280.1 287.9 296.2 305.0 314.3 324.4 335.2 346.9 359.6 373.7 389.2

33 204.7 209.1 213.7 218.4 223.3 228.4 233.7 239.1 244.9 250.8 257.1 263.6 270.4 277.7 285.3 293.3 301.9 311.0 320.7 331.1 342.4 354.7 368.1 383.0 399.5

34 207.9 212.4 217.0 221.9 226.9 232.1 237.5 243.1 248.9 255.1 261.5 268.2 275.2 282.7 290.5 298.8 307.7 317.1 327.3 338.2 350.0 362.9 377.1 392.8 410.4

35 211.1 215.7 220.4 225.4 230.5 235.8 241.3 247.1 253.1 259.4 266.0 272.9 280.1 287.8 295.9 304.5 313.7 323.5 334.1 345.5 357.9 371.5 386.4 403.1 422.0

36 214.4 219.1 223.9 229.0 234.2 239.6 245.3 251.2 257.4 263.8 270.6 277.7 285.2 293.1 301.5 310.4 320.0 330.2 341.2 353.1 366.1 380.4 396.3 414.1 434.4

37 217.7 222.5 227.4 232.6 238.0 243.5 249.3 255.4 261.7 268.3 275.3 282.6 290.4 298.5 307.2 316.5 326.4 337.0 348.6 361.1 374.8 389.9 406.8 425.9 447.8

38 221.1 226.0 231.0 236.3 241.8 247.5 253.4 259.7 266.2 273.0 280.1 287.7 295.7 304.1 313.2 322.8 333.1 344.2 356.2 369.4 383.8 399.9 417.9 438.4 462.2

39 224.5 229.5 234.7 240.1 245.7 251.5 257.6 264.0 270.7 277.7 285.1 292.9 301.2 309.9 319.3 329.2 340.0 351.6 364.2 378.1 393.3 410.4 429.7 451.8 477.8

40 228.0 233.1 238.4 244.0 249.7 255.7 261.9 268.5 275.4 282.6 290.2 298.3 306.8 315.9 325.6 336.0 347.2 359.3 372.6 387.2 403.4 421.6 442.3 466.4 494.9

41 231.6 236.8 242.2 247.9 253.8 259.9 266.3 273.1 280.1 287.6 295.4 303.8 312.6 322.0 332.1 342.9 354.6 367.4 381.4 396.8 414.0 433.5 455.9 482.1 513.8

42 235.2 240.6 246.1 251.9 257.9 264.2 270.8 277.8 285.0 292.7 300.8 309.4 318.6 328.4 338.8 350.2 362.4 375.8 390.5 406.9 425.3 446.2 470.5 499.4 534.8

43 238.9 244.4 250.1 256.0 262.2 268.7 275.4 282.6 290.1 298.0 306.4 315.3 324.8 334.9 345.9 357.7 370.5 384.6 400.2 417.6 437.3 459.9 486.5 518.5 558.6

44 242.7 248.3 254.1 260.2 266.5 273.2 280.1 287.5 295.2 303.4 312.1 321.3 331.1 341.7 353.1 365.5 379.0 393.9 410.4 429.0 450.1 474.7 503.9 539.7 585.8

45 246.5 252.2 258.2 264.4 271.0 277.8 285.0 292.5 300.5 309.0 317.9 327.5 337.8 348.8 360.7 373.7 387.9 403.6 421.2 441.1 463.9 490.7 523.1 563.7 617.7

46 250.4 256.3 262.4 268.8 275.5 282.5 289.9 297.7 305.9 314.7 324.0 333.9 344.6 356.1 368.6 382.2 397.2 413.9 432.6 454.0 478.8 508.3 544.5 591.1 655.9

47 254.4 260.4 266.7 273.3 280.1 287.4 295.0 303.0 311.5 320.6 330.2 340.6 351.7 363.7 376.8 391.2 407.0 424.8 444.8 467.9 495.0 527.7 568.7 623.3 703.6

48 258.5 264.6 271.1 277.8 284.9 292.3 300.2 308.5 317.3 326.7 336.7 347.5 359.1 371.6 385.4 400.5 417.4 436.3 457.9 482.9 512.7 549.3 596.4 661.9 766.5

49 262.6 269.0 275.6 282.5 289.8 297.4 305.5 314.1 323.2 333.0 343.4 354.6 366.7 379.9 394.4 410.4 428.3 448.6 471.9 499.2 532.2 573.7 628.8 710.0 857.9

50 266.9 273.4 280.1 287.3 294.8 302.7 311.0 319.9 329.4 339.5 350.3 362.0 374.7 388.6 403.8 420.8 439.9 461.7 487.0 517.1 554.0 601.6 667.8 773.4 1020.3

51 271.2 277.9 284.8 292.2 299.9 308.1 316.7 325.9 335.7 346.2 357.5 369.7 383.0 397.6 413.8 431.8 452.3 475.8 503.4 536.8 578.6 634.3 716.3 865.4 >1020.3

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