rDNase Set
1 Contents
rDNase Set
REF 740963
rDNase, RNase-free 1 vial (size F)
(lyophilized)
Reaction Buffer for rDNase 7 mL
2 Product description
The rDNase Set is intended for digestion of DNA in solutions (e.g.,
prepurified RNA samples) or for use with NucleoSpin® RNA kits
(on‑column DNA digest). The optimized Reaction Buffer for rDNase
is highly recommended for best DNase performance.
User option A: DNA digest in solution
The amount of rDNase and Reaction Buffer for rDNase is sufficient
for DNA digestion in a total of 50 mL solution (e.g., 500 x 100 µL).
For preparation and storage of working solutions see section 4. For
recommended protocol see section 6.
User option B: On‑column digest with NucleoSpin® RNA kits
The amount of rDNase and Reaction Buffer for rDNase is sufficient
for the following numbers of on‑column DNA digestions:
NucleoSpin® RNA 50 preps
NucleoSpin® RNA XS 90 preps
NucleoSpin® RNA Midi 100 preps
NucleoSpin® 8 RNA 240 preps
NucleoSpin® 96 RNA 240 preps
NucleoSpin® miRNA 60 preps
NucleoSpin® miRNA Plasma 120 preps
NucleoSpin® RNA/Protein 60 preps
NucleoSpin® TriPrep 60 preps
NucleoSpin® RNA Blood 60 preps
NucleoSpin® RNA Blood Midi 25 preps
NucleoSpin® 8 RNA Blood 240 preps
NucleoSpin® 96 RNA Blood 240 preps
NucleoSpin® RNA Plant 50 preps
NucleoSpin® RNA Plant and Fungi 40 preps
NucleoSpin® RNA Stool 85 preps
NucleoSpin® totalRNA FFPE 120 preps
NucleoSpin® totalRNA FFPE XS 240 preps
For preparation and storage of working solutions see section 4. For
protocol please refer to the respective user manual.
3 rDNase specifications
Source: The rDNase is DNase I from bovine pancreas,
recombinantly produced in Pichia pastoris without using any animal
cells or other material derived from animals
RNase activity: Not detectable. RNase activity was tested using a
cleavable fluorescent labeled RNase substrate. No RNase activity
was detectable after one hour incubation time.
RNA integrity: Measurements with the Agilent 2100 Bioanalyzer
show, that RNA integrity is untouched by the treatment with
MACHEREY‑NAGEL rDNase.
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199 ·
[email protected] · www.mn-net.com
MACHEREY-NAGEL – 03/2019, Rev. 14
4 Storage conditions and preparation of working solution
rDNase, RNase-free: Store lyophilized rDNase at +4 °C upon arrival
(stable for up to one year).
Reconstitution for user option A: DNA digest in solution
Before first use add 550 µL of RNase-free H2O (see below) to the
rDNase vial (size F) and incubate for 1 min at room temperature.
Gently swirl the vial to completely dissolve the rDNase. Be careful
not to mix rDNase vigorously. rDNase is sensitive to mechanical
agitation.
Reconstitution for user option B: On‑column digest with
NucleoSpin® RNA kits
For NucleoSpin® RNA Blood kit, NucleoSpin® totalRNA FFPE
kits, NucleoSpin® miRNA Plasma kit, and NucleoSpin® miRNA
kit, dissolve rDNase (Size F) in the delivered volume of Reaction
Buffer for rDNase (7 mL). Store and use rDNase according to the
respective user manual.
For correct use of rDNase and Reaction Buffer for rDNase in the
other NucleoSpin® RNA kits mentioned in section 2 please refer to
the respective manual for detailed information. In particular check
the vial size of the rDNase mentioned in the manual protocol:
If the kit contained rDNase vials size C, then dissolve the rDNase
from this set in 12.5 volumes of RNase-free H2O indicated in the kit
manual.
If the kit contained rDNase vials size D, then dissolve the rDNase
from this set in 5 volumes of RNase-free H2O indicated in the kit
manual.
After dissolving the rDNase proceed according to the manual
protocol.
Storage after reconstitution: Dispense reconstituted rDNase working
solution into aliquots and store at -20 °C. The frozen working solution
is stable for 6 months. Do not freeze / thaw the aliquots more than
three times.
5 Safety instructions
rDNase contains hazardous contents. Wear gloves and goggles!
Only harmful features must not be labeled with H and P phrases up to 125 mL or
125 g.
Mindergefährliche Eigenschaften müssen bis 125 mL oder 125 g nicht mit H- und P-Sätzen
gekennzeichnet werden.
GHS Hazard Precaution
Component Hazard contents
symbol phrases phrases
Inhalt Gefahrstoff GHS-Symbol H-Sätze P-Sätze
rDNase rDNase 90–100 % 334 261sh,
(Enzym) rDNase 342+311
90–100 %
CAS 9003‑98‑9 DANGER
GEFAHR
Hazard phrases
H 334 May cause allergy or asthma symptoms or breathing difficulties
if inhaled.
Kann bei Einatmen Allergie, asthmaartige Symptome oder
Atembeschwerden verursachen.
Precaution phrases
P 261sh Avoid breathing dust/vapors.
Einatmen von Staub/Dampf vermeiden.
P 342+311 If experiencing respiratory symptoms: Call a POISON
CENTER/doctor.
Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM/Arzt
anrufen.
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199 ·
[email protected] · www.mn-net.com
MACHEREY-NAGEL – 03/2019, Rev. 14
For further information please see Material Safety Data Sheets
(www.mn‑net.com).
Weiterführende Informationen finden Sie in den Sicherheitsdatenblättern (www.mn‑net.com).
The symbol shown on labels refers to further safety information in this section.
Das auf Etiketten dargestellte Symbol weist auf weitere Sicherheitsinformationen dieses
Kapitels hin.
6 Protocol for DNA digestion in solution
Several commonly used RNA purification methods co-purify DNA
to a considerable extent (e.g., phenol based RNA purification). This
often requires a subsequent removal of contaminating DNA and
clean up of the RNA from the reaction mixture.
DNA digestion in solution can efficiently destroy contaminating DNA.
Subsequent repurification of the RNA (in order to remove buffer,
salts, DNase, and digested DNA) is usually required.
The rDNase Set, containing high quality, recombinant RNase-free
DNase (rDNase) and Reaction Buffer facilitates such a digestion in
solution in order to remove even traces of contaminating DNA.
1 Digest DNA (Reaction Setup)
Prepare enzyme-buffer premix in a ratio of 1:10. Example:
Add 1 μL reconstituted rDNase to 10 μL Reaction Buffer for
rDNase.
Add 1/10 volume of the enzyme-buffer premix to the crude
RNA solution (e.g., add 1 μL of the enzyme-buffer premix to
10 μL RNA). In case of NucleoSpin 8/96 RNA and NucleoSpin
8/96 RNA Blood kits add 2/10 volume of the enzyme-buffer
premix to the crude RNA solution.
Note: The crude RNA solution should be of low RNase activity,
otherwise the RNA quality may be affected during incubation.
2 Incubation
Incubate for 10 min at 37 °C.
3 Repurification of RNA
Repurify RNA using a suitable cleanup procedure, e.g., with
the NucleoSpin® RNA Clean‑up XS (REF 740903).
7 Product use restriction / warranty
The rDNase Set is developed, designed, and sold for research
purpose only.
All goods have to be checked immediately upon receipt. Damage
claims are only acceptable in writing within 8 working days of receipt
of all the goods. In case of legitimate claims the buyer can only
require replacement of the goods. If replacement is not possible,
the buyer has the right to choose between alternative products with
same value or refund. The buyer cannot claim further compensation.
All returns must first be authorized by us in writing. We offer a one
year warranty from date of delivery that our products will conform
to applicable specifications set forth in the product specifications if
not sold to persons set forth in § 13 of German Civil Code. In such a
case, the provisions of the German Civil Code shall be valid.
This warranty does not cover defects in or damage to the products
which are due to improper installation or maintenance, misuse,
neglect or any use other than ordinary commercial application.
Any discharge from liability will be void if a defect results from a
heavily negligent or intentional breach of contract or if the buyer will
be bodily injured as a result from a negligent or intentional breach.
The seller shall not be liable for the goods being fit for a particular
purpose unless otherwise agreed upon, to which the buyer intends
to put them.
This warranty is strictly exclusive. Any further damage compensation
is impossible.
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199 ·
[email protected] · www.mn-net.com
MACHEREY-NAGEL – 03/2019, Rev. 14
MACHEREY‑NAGEL MAKES no other warranty of any kind
whatsoever, and SPECIFICALLY DISCLAIMS AND EXCLUDES ALL
OTHER WARRANTIES OF ANY KIND OR NATURE WHATSOEVER;
DIRECTLY OR INDIRECTLY; EXPRESS OR IMPLIED;
INCLUDING; WITHOUT LIMITATION; AS TO THE SUITABILITY;
REPRODUCIBILITY; DURABILITY; FITNESS FOR A PARTICULAR
PURPOSE OR USE; MERCHANTABILITY; CONDITION; OR
ANY OTHER MATTER WITH RESPECT TO MACHEREY‑NAGEL
PRODUCTS OR THE SALE OF MACHEREY‑NAGEL PRODUCTS.
If not superseded by these terms the current terms and conditions
for the sale of goods (export version) are additionally valid.
A026849 / 0391.1
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199 ·
[email protected] · www.mn-net.com
MACHEREY-NAGEL – 03/2019, Rev. 14
