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PCR-report HW

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7 views3 pages

PCR-report HW

K

Uploaded by

kpeeh789
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Experiment 1

Polymerase Chain Reaction (PCR) and Analysis of DNA

Bashayer
Name:____ Alkandari
____________________Student 2211113047
Number:_______________

Group 34 22/9
Group Identification:_________________________________Date:_____________is

Raeda Khanafer Lab Mark:_______________


Group Instructor (Initials):_________________

The following sheet must be filled with data collected from your experiment then
answer the exercise questions and submit your report in the MS Teams- Biochemistry
Practical-Y2 (2021-2022)

Results:

Attach gel picture in the below square

Record the number and size (small ----> big) of the prominent bands visible on
the gel picture in the sample lane (Note: The smallest band is a 100 bp).

Practical 1
Page 1
Well 2
Number of Well 1
Size in bp [amplified sample
ladder bands [Ladder bands] band]

1 800

2 700

3 600

4 500

5 400

6 300

7 200

8 100
( +)

500
The size of the amplified band: _ _ _ _ _ _ _ _ _ _ bp.

Conclusions:

500
PCR amplified a band of _ _ _ _ _ _ _ _ _ _ bp from  DNA template.

Exercise Questions:

1. The number of amplified pieces of DNA equals ____ after five cycles of PCR.
A) 5
B) 10
C) 25
D) 32
2. The figure below shows the DNA fingerprints of four individuals.
Assuming that large fragments run more slowly in the gel below, which individual
has the largest DNA fragment?
Practical 1
Page 2
A) Individual 1
B) Individual 2
C) Individual 3
D) Individual 4

3. Which of the following best describes the mixture placed in a PCR vial at the
beginning of the PCR procedure?

A) the original DNA sample, DNA polymerase, fluorescent-tagged bases


and buffer solution

B) the original DNA sample, endonucleases, the four nucleotide bases and
primers

C) the original DNA sample, DNA polymerase, the four nucleotide bases
and primers

D) the original DNA sample, DNA polymerase, adenine, thymine, cytosine


and guanine

Practical 1
Page 3

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