UG/CBCS/B.Sc./Hons./6th Sem.
/Botany/BOTCC14/2022
UNIVERSITY OF NORTH BENGAL
B.Sc. Honours 6th Semester Examination, 2022
CC14-BOTANY
Time Allotted: 2 Hours Full Marks: 40
The figures in the margin indicate full marks.
GROUP-A
1. Answer any five questions from the following: 1×5 = 5
(a) What is explant?
(b) What is meant by haploid protoplast?
(c) What is meant by totipotency of a cell?
(d) What is Ti plasmid and where is it found?
(e) Give the origin and recognition sequence of a frequent cutter.
(f) What is shuttle vector?
(g) What is multiple cloning site?
(h) What is the significance of DNA ligase in recombinant DNA technology?
GROUP-B
2. Answer any three questions from the following: 5×3 = 15
(a) What is micropropagation? Discuss in detail the various steps of 1+4 = 5
micropropagation.
(b) Distinguish between: 2 12 +2 12 =5
(i) Organogenesis and embryogenesis
(ii) Selectable markers and reporter genes.
(c) Name and describe one each of prokaryotic and eukaryotic vector. 2 12 +2 12 =5
(d) Describe the method of construction of cDNA library. 5
(e) What are restriction endonucleases? Give an account of classification of 1+4 = 5
restriction endonucleases.
6009 1 Turn Over
UG/CBCS/B.Sc./Hons./6th Sem./Botany/BOTCC14/2022
GROUP-C
3. Answer any two questions from the following: 10×2 = 20
(a) Describe the generation of a transgenic plant by means of an Agrobacterium 10
transformation system with suitable diagram.
(b) Define PCR. Discuss in detail the steps involved in a PCR reaction with suitable 2+8 = 10
diagram.
(c) What is protoplast culture? What is the main objective of protoplast culture? 2+2+6=10
Describe the available methods for isolation and purification of protoplast.
(d) Write short notes on: 5+5 = 10
(i) Golden rice
(ii) Edible vaccines.
——×——
6009 2
