+2 Practical EM
+2 Practical EM
Preliminary tests
1 Odour: Bitter almond odour May be benzaldehyde
Odour of the organic
compound
2 Test with litmus paper: No colour change Absence of
Touch the Moist litmus carboxylic acid ,
paper with an organic phenol and amine
compound
3 Action with sodium No brisk effervescence Absence of a
bicarbonate: carboxylic acid.
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube. Add
2 or 3 drops (or a pinch of
solid) of an organic
compound to it
4 Action with Borsche’s Red precipitate Presence of an
reagent: aldehyde or ketone
Take a small amount of an
organic compound in a test
tube. Add 3 ml of Borsche’s
reagent, 1 ml of Conc HCl
to it, then warm the mixture
gently and cool it
5 Charring test: No Charring takes place with Absence of
Take a small amount of an smell of burnt sugar carbohydrate
organic compound in a dry
test tube. Add 2 ml of conc
H2SO4 to it, and heat the
mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an
Take small amount of the aromatic compound
organic compound in a
Nickel spatula and burn it in
Bunsen flame.
Tests for an unsaturation
7 Test with bromine water: Decolourisation with Unsaturated aromatic
Take small amount of the formation of white precipitate compound
organic compound in a test
tube add 2 ml of distilled
water to dissolve it. To this
solution add few drops of
bromine water and shake it
well
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of the
organic compound in a test
tube add 2 ml of distilled
water to dissolve it. To this
solution add few drops of
very dilute alkaline KMnO4
solution and shake it well.
Test for aldehydes
1 Tollen’s reagent test: Shining silver mirror is Presence of an
Take 2 ml of Tollen’s formed. aldehyde
reagent in a clean dry test
tube. Add 3-4 drops of an
organic compound (or 0.2 g
of solid) to it, and warm the
mixture on a water bath for
about 5 minutes
2 Fehling’s test: Red precipitate is formed Presence of an
Take 1 ml each of Fehling’s aldehyde
solution A and B are taken
in a test tube. Add 4-5 drops
of an organic compound (or
0.2g of solid) to it, and
warm the mixture on a
water bath for about 5
minutes
Report:
The given organic compound contains (Benzaldehyde)
i) Aromatic compound
ii) unsaturated compound
iii) functional group -Aldehyde
2) Cinnmaldehyde
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus paper: No colour change Absence of carboxylic
Touch the Moist litmus acid , phenol and amine
paper with an organic
compound
3 Action with sodium No brisk effervescence Absence of a carboxylic
bicarbonate: acid.
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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organic compound to it
4 Action with Borsche’s yellow or orange or red Presence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
5 Charring test: No Charring takes place Absence of carbohydrate
Take a small amount of with smell of burnt sugar
an organic compound in
a dry test tube. Add 2 ml
of conc H2SO4 to it, and
heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of the compound
organic compound in a
Nickel spatula and burn it
in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
water: formation of white compound
Take small amount of the precipitate
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of bromine
water and shake it well
8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of very dilute
alkaline KMnO4 solution
and shake it well.
Test for aldehydes
1 Tollen’s reagent test: Shining silver mirror is Presence of an aldehyde
Take 2 ml of Tollen’s formed.
reagent in a clean dry test
tube.Add 3-4 drops of an
organiccompound (or 0.2
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
Page 3
g of solid) to it, and
warm the mixture on a
water bath for about 5
minutes
2 Fehling’s test: Red precipitate is formed Presence of an aldehyde
Take 1 ml each of
Fehling’s solution A and
B are taken in a test tube.
Add 4-5 drops of an
organic compound (or
0.2g of solid) to it, and
warm the mixture on a
water bath for about 5
minutes
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) functional group -Aldehyde
3. Acetophenone
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus No colour change Absence of carboxylic
paper: acid , phenol and amine
Touch the Moist litmus
paper with an organic
compound
3 Action with sodium No brisk effervescence Absence of a carboxylic
bicarbonate: acid.
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s yellow or orange or red Presence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
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5 Charring test: No Charring takes place Absence of carbohydrate
Take a small amount of with smell of burnt sugar
an organic compound in
a dry test tube. Add 2
ml of conc H2SO4 to it,
and heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of compound
the organic compound
in a Nickel spatula and
burn it in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
compound
water: formation of white
Take small amount of precipitate
the organic compound
in a test tube add 2 ml
of distilled water to
dissolve it. To this
solution add few drops
of bromine water and
shake it well
8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water to
dissolve it. To this
solution add few drops
of very dilute alkaline
KMnO4 solution and
shake it well.
Test for ketones
1 Legal’s test: Red colouration Presence of a ketone.
A small amount of the
substance is taken in a
test tube.1 ml sodium
nitro prusside solution is
added. Then sodium
hydroxide solution is
added dropwise
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) functional group- Ketone
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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4. Benzophenone
S.no Experiment Observation Inference
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus No colour change Absence of carboxylic
paper: acid , phenol and amine
Touch the Moist litmus
paper with an organic
compound
3 Action with sodium No brisk effervescence Absence of a carboxylic
bicarbonate: acid.
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s yellow or orange or red Presence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
5 Charring test: No Charring takes place Absence of carbohydrate
Take a small amount of with smell of burnt sugar
an organic compound in
a dry test tube. Add 2
ml of conc H2SO4 to it,
and heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of compound
the organic compound
in a Nickel spatula and
burn it in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
water: formation of white compound
Take small amount of precipitate
the organic compound
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
Page 6
in a test tube add 2 ml
of distilled water to
dissolve it.To this
solution add few drops
of bromine water and
shake it well
8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water to
dissolve it. To this
solution add few drops
of very dilute alkaline
KMnO4 solution and
shake it well.
Test for Ketone
1 Legal’s test: Red colouration Presence of a ketone.
A small amount of the
substance is taken in a
test tube. 1 ml sodium
nitro prusside solution is
added. Then sodium
hydroxide solution is
added dropwise.
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) functional group - Ketone
5. Benzoic Acid
Preliminary tests
1 Odour: No characteristic odour Absence of amine,
Odour of the organic phenol, benzaldehyde
compound and ester
2 Test with litmus paper: Blue litmus turns red May be a carboxylic
Touch the Moist litmus acid
paper with an organic or phenol
compound
3 Action with sodium Brisk effervescence Presence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
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4 Action with Borsche’s No yellow or orange or red Absence of an aldehyde
reagent: precipitate or
Take a small amount of ketone
an organic compound in a
test tube. Add 3 ml of
Borsche’s reagent, 1 ml of
Conc HCl to it, then warm
the mixture gently and
cool it
5 Charring test: No Charring takes place with Absence of carbohydrate
Take a small amount of smell of burnt sugar
an organic compound in a
dry test tube. Add 2 ml of
conc H2SO4 to it, and heat
the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of the compound
organic compound in a
Nickel spatula and burn it
in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
water: formation of white compound
Take small amount of the precipitate
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of bromine
water and shake it well
8 Test with KMnO4 Pink colour of KMnO4 Substance is unsaturated
solution: solution is decolourised
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of very dilute
alkaline KMnO4 solution
and shake it well.
Test for Carboxylic acid
1 Esterification reaction: A Pleasant fruity odour is Presence of carboxylic
Take 1 ml (or a pinch of noted group
solid) of an organic
compound in a clean test
tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops
of conc. sulphuric acid to
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Page 8
it. Heat the reaction
mixture strongly for about
5 minutes. Then pour the
mixture into a beaker
containing dil. Sodium
carbonate solution and
note the smell.
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) functional group -Carboxylic acid
6. Cinnamic acid
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus Blue litmus turns red May be a carboxylic acid
paper: or phenol
Touch the Moist litmus
paper with an organic
compound
3 Action with sodium Brisk effervescence Presence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s No yellow or orange or red Absence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
5 Charring test: No Charring takes place Absence of carbohydrate
Take a small amount of with smell of burnt sugar
an organic compound in
a dry test tube. Add 2 ml
of conc H2SO4 to it, and
heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of compound
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the organic compound
in a Nickel spatula and
burn it in Bunsen flame.
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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7. Salicylic acid
S.no Experiment Observation Inference
Preliminary tests
1 Odour: Phenolic odour May be phenol
Odour of the organic
compound
2 Test with litmus paper: Blue litmus turns red May be a carboxylic
Touch the Moist litmus acid or phenol
paper with an organic
compound
3 Action with sodium Brisk effervescence Presence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s No yellow or orange or red Absence of an aldehyde
reagent: precipitate or ketone
Take a small amount of an
organic compound in a
test tube. Add 3 ml of
Borsche’s reagent, 1 ml of
Conc HCl to it, then warm
the mixture gently and
cool it
5 Charring test: No Charring takes place with Absence of carbohydrate
Take a small amount of an smell of burnt sugar
organic compound in a
dry test tube. Add 2 ml of
conc H2SO4 to it, and heat
the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of the compound
organic compound in a
Nickel spatula and burn it
in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
water: formation of white precipitate compound
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of bromine
water and shake it well
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8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of very dilute
alkaline KMnO4 solution
and shake it well.
Test for Carboxylic acid
1 Esterification reaction: A Pleasant fruity odour is Presence of carboxylic
Take 1 ml (or a pinch of noted group
solid) of an organic
compound in a clean test
tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops
of conc. sulphuric acid to
it. Heat the reaction
mixture strongly for about
5 minutes.Then pour the
mixture into a beaker
containing dil. Sodium
carbonate solution and
note the smell.
Tests for Phenol
2. Neutral FeCl3 test: Violet colouration is seen Presence of phenol
Take 1 ml of neutral ferric
chloride solution is taken
in a dry clean test tube.
Add 2 or 3 drops (or a
pinch of solid) of organic
compound to it. If no
colouration occurs add 3
or 4 drops of alcohol
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) Carboxylic acid and phenolic functional groups.
8. Aniline
S.no Experiment Observation Inference
Preliminary tests
1 Odour: Fish odour May be an amine
Odour of the organic
compound
2 Test with litmus paper: Red litmus turns blue May be an amine
Touch the Moist litmus
paper with an organic
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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compound
3 Action with sodium Brisk effervescence Presence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s No yellow or orange or red Absence of an aldehyde
reagent: precipitate or
Take a small amount of ketone
an organic compound in a
test tube. Add 3 ml of
Borsche’s reagent, 1 ml of
Conc HCl to it, then
warm the mixture gently
and cool it
5 Charring test: No Charring takes place with Absence of carbohydrate
Take a small amount of smell of burnt sugar
an organic compound in a
dry test tube. Add 2 ml of
conc H2SO4 to it, and
heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of the compound
organic compound in a
Nickel spatula and burn it
in Bunsen flame.
Tests for an unsaturation
7 Test with bromine Decolourisation with Unsaturated aromatic
water: formation of white precipitate compound
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of bromine
water and shake it well
8 Test with KMnO4 Pink colour of KMnO4 Substance is
solution: solution is decolourised unsaturated
Take small amount of the
organic compound in a
test tube add 2 ml of
distilled water to dissolve
it. To this solution add
few drops of very dilute
alkaline KMnO4 solution
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
Page 13
and shake it well.
Test for an amine.
1 Dye test: Scarlet red dye is obtained Presence of an aromatic
Take A small amount of primary amine
an organic substance in a
clean test tube, add 2 ml
of HCl to dissolve it. Add
few crystals of NaNO2,
and cool the mixture in
ice bath. Then add 2 ml of
ice cold solution of β-
naphthol in NaOH
Report:
The given organic compound contains
i) Aromatic compound
ii) unsaturated compound
iii) functional group -Amine
9.Urea
S.no Experiment Observation Inference
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus No colour change Absence of carboxylic
paper: acid , phenol and amine
Touch the Moist litmus
paper with an organic
compound
3 Action with sodium Brisk effervescence Presence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium
bi carbonate solution in
a test tube. Add 2 or 3
drops (or a pinch of
solid) of an organic
compound to it
4 Action with Borsche’s No yellow or orange or red Absence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
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5 Charring test: No Charring takes place Absence of carbohydrate
Take a small amount of with smell of burnt sugar
an organic compound in
a dry test tube. Add 2
ml of conc H2SO4 to it,
and heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burn with sooty flame Presence of an aromatic
Take small amount of compound
the organic compound
in a Nickel spatula and
burn it in Bunsen flame.
Tests for an unsaturation
7 Test with bromine No Decolourisation takes Substance is saturated.
water: place
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water to
dissolve it. To this
solution add few drops
of bromine water and
shake it well
8 Test with KMnO4 No Decolourisation takes Substance is saturated
solution: place
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water
to dissolve it. To this
solution add few drops
of very dilute alkaline
KMnO4 solution and
shake it well.
Test for diamide
1 Biuret test: Violet colour is appeared presence of a diamide
Take A small amount of
an organic compound in
a test
tube. Heat strongly and
then allow to cool.
Dissolve the residue
with 2 ml of water.
To this solution Add 1
ml of dilute copper
sulphate solution
and few drops of 10%
NaOH solution drop by
drop.
P.KASIVISVANATHAN,GHSS.MANMANGALAM.
Page 15
Report:
The given organic compound contains
i) Aliphatic compound
ii) saturated compound
iii) functional group - diamide
10. Glucose
S.no Experiment Observation Inference
Preliminary tests
1 Odour: No characteristic odour Absence of
Odour of the organic amine,phenol,benzaldehyde
compound and ester
2 Test with litmus No colour change Absence of carboxylic
paper: acid , phenol and amine
Touch the Moist litmus
paper with an organic
compound
3 Action with sodium No Brisk effervescence Absence of a carboxylic
bicarbonate: acid
Take 2 ml of saturated
sodium bi carbonate
solution in a test tube.
Add 2 or 3 drops (or a
pinch of solid) of an
organic compound to it
4 Action with Borsche’s yellow or orange Presence of an aldehyde or
reagent: precipitate ketone
Take a small amount of
an organic compound in
a test tube. Add 3 ml of
Borsche’s reagent, 1 ml
of Conc HCl to it, then
warm the mixture gently
and cool it
5 Charring test: Charring takes place with Presence of carbohydrate
Take a small amount of smell of burnt sugar
an organic compound in
a dry test tube. Add 2
ml of conc H2SO4 to it,
and heat the mixture.
Tests for Aliphatic or Aromatic nature
6 Ignition test: Burns with non sooty flame Presence of an aliphatic
Take small amount of compound
the organic compound
in a Nickel spatula and
burn it in Bunsen flame.
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Page 16
Tests for an unsaturation
7 Test with bromine No Decolourisation takes Substance is saturated.
water: place
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water to
dissolve it. To this
solution add few drops
of bromine water and
shake it well
8 Test with KMnO4 No Decolourisation takes Substance is saturated
solution: place
Take small amount of
the organic compound
in a test tube add 2 ml
of distilled water
to dissolve it. To this
solution add few drops
of very dilute alkaline
KMnO4 solution and
shake it well.
Test for carbohydrates
1 Molisch’s test: Violet or purple ring is Presence of carbohydrate
Take A small amount of formed at the junction of
an organic compound in the two liquids.
a test
tube. It is dissolved in 2
ml of water. Add 3-4
drops of alpha naphthol
to it.Then add conc
H2SO4 through the sides
of test tube carefully.
2 Osazone test: Yellow crystals are Presence of carbohydrate
Take a small amount of obtained
an organic compound in
a test tube. Add 1 ml of
phenyl hydrazine
solution and heat the
mixture for about 5
minutes on a boiling
water bath
Report:
The given organic compound contains
i) Aliphatic compound
ii) saturated compound
iii) functional group - aldehyde or ketone
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