5990 3699en
5990 3699en
5990 3699en
Dear Valued Customer, Now, more than ever, labs are under pressure to do more work with fewer resources, without sacrificing quality. At Agilent, our job is to recognize customers' challenges and provide the tools and support they need to succeed in these challenging times. This is why we are pleased to present the 2009-2010 Edition of the Agilent Instrument & Accessories Catalog. It's more than a collection of hardware and specifications. It represents years of working closely with our customers and thoughtful product development and enhancement to meet your real-world requirements. Inside you'll find Agilent instruments, accessories and software designed for productivity and precision. We encourage you to contact your local Agilent office or Authorized Agilent Distributor with any questions, to request a quotation or to place an order. We also encourage you to visit www.chem.agilent.com for the latest product information, applications and special offers. Sincerely,
Lon Justice Vice President and General Manager Global Sales, Marketing and Services Life Sciences and Chemical Analysis
Table of Contents
Agilent Solutions for Chemical Analysis and Life Sciences.......................................2 Agilent GC and GC/MS Solutions...................................................................................4 Agilent LC and LC/MS Solutions..................................................................................32 Agilent Automation Solutions .......................................................................................60 Agilent Spectroscopy and Microscopy Solutions ......................................................66 Agilent Particle Analyzer Solutions..............................................................................68 Agilent Capillary Electrophoresis Solutions................................................................72 Agilent Bioanalyzer Solutions .......................................................................................74 Agilent ICP-MS Solutions...............................................................................................80 Agilent Microarray Solutions.........................................................................................86 Agilent PCR and QPCR Solutions..................................................................................88 Agilent Software and Informatics Solutions...............................................................92 Agilent Service and Support for Instrument Systems.............................................112 Agilent Columns and Supplies ....................................................................................114
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Food
From high-volume screening of vegetables for pesticides through detection of genetically-modified products, Agilent understands the analytical needs of food producers, shippers and regulators. When a new contaminant appears, we deploy substantial resources to quickly help customers develop robust, reliable methods.
Forensics
Because the futures of world class athletes and many other individuals hinge on drug testing, its critical that those doing the testing have the highest level of confidence in their results. Forensics analysts worldwide have grown to depend on Agilent tools for accuracy, reliability and speed in this high-stakes, high-throughput field. Our best selling GC, GC/MS and popular LC and LC/MS are workhorses in forensics labs.
Environmental
Agilent offers more than 40 years of environmental analysis and regulatory expertise. We help government and private labs with a full range of assays, from routine testing of soils for heavy metals through detection of pharmaceuticals in groundwater in concentrations down to parts-per-trillion.
Lab Informatics
The way labs generate and store data profoundly affect their efficiency. Agilent offers a comprehensive selection of software, ranging from industry-leading instrument control capabilities in our data systems through an innovative operating system for the laboratory that provides a fully searchable archive for all data, regardless of file type.
Pharmaceutical
Drug discovery, development and manufacturing requires the accuracy, sensitivity and high throughput of other analytical applications, with the added demands of regulatory recordkeeping and validation requirements. Agilent provides a potent combination of rugged, high-throughput tools along with unmatched compliance services.
Genomics
Agilent is a global leader in microarrays, scanners, and reagents used in a wide variety of genomic-based disease research experiments. We offer a wide range of catalog arrays and a highly-developed capability to produce custom arrays thanks to ink jet-based SurePrint fabrication and the eArray on-line design tool. All Agilent microarrays feature highly-sensitive, selective 60 mer probes. With as many as eight arrays printed on a standard 1 inch x 3 inch slide, the cost per experiment becomes very affordable.
Lab Automation
To meet the increasing demand for more throughput and automation, Agilent has substantially expanded its lab automation offerings. The portfolio of liquid handlers and microplate processors are designed to streamline high-volume life science workflows. Agilent is also continually upgrading its advanced autosamplers for GC, GC/MS, LC and LC/MS, adding functionality and speed to reflect the performance of its advanced instruments.
Proteomics Metabolomics
Studying metabolomics in biological samples presents many challenges. Molecules are constantly entering, leaving or changing within the metabolome, underscoring the need for speed, accuracy and powerful interpretation capabilities in looking at chemical profile snapshots. Agilents GC/MS and LC/MS portfolios align very well with the needs of metabolomics researchers, along with leading bioinformatics offerings for identification and statistical analysis. Your proteomics research goals need to be met by complete, optimized and accessible workflows for fast, accurate and reproducible results. Agilent's portfolio of LC/MS and bioinformatics systems, multiple affinity protein removal columns, and OFFGEL electrophoresis provides the highest analytical performance with unprecedented flexibility. Our robust, interchangeable workflows simplify setups and let you quickly switch between different methodologies to complete your research faster, with greater confidence.
Faster oven cool down and faster GC/MS oven ramps Robust backflush capability Advanced automation capabilities
Easier Operation
Powerful, chromatographer-friendly software simplifies method setup and system operation and minimizes training time Practical, time-saving design features speed up and simplify routine maintenance Automated monitoring and diagnostics software tracks consumables usage, monitors chromatographic quality and alerts you to problems before they happen Easy, direct method transfer from Agilent 6890 GC
Highly flexible EPC design enables more sophisticated analyses Optional third detector (TCD) can speed up complex gas analyses, and allows more types of analyses to be run on a single GC Fully compatible with Agilent Capillary Flow Technology New Multimode Inlet used as split/splitless inlet or with temperature programming and large volume injection capabilities
Description 7890 Gas Chromatograph Mainframe 7890 GC bundle for 5975 Series GC/MSD, includes split/splitless inlet and MSD interface
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Packed
For glass and metal 1/4 and 1/8 inch packed columns and 0.53 mm megabore capillary columns
Volatiles Interface
Facilitates trace level detection of prevaporized samples
Cool On-Column
Temperature programmable cool on-column for direct liquid injection minimizes discrimination during injection and separates low boiling point constituents selectively
Turn Top The Easiest Way to Change Inlet Liners (7890 GC only)
Agilent's Turn Top Inlet Sealing System for 7890 GC is already built into Agilent's Split/Splitless and Multimode inlets; The user can safely and reliably change an inlet liner in as little as 30 seconds without tools
Valves
The reproducible way to introduce gaseous samples to 7890 and 6890 GCs; Call for details
Flip Top The Easiest Way to Change Inlet Liners (6890 and 6850 GC only)
Agilent's Flip Top Inlet Sealing System for the 6890/6850 GC is a device designed to allow the user to safely and reliably change an inlet liner in as little as 30 seconds without tools; Available exclusively from Agilent
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Multimode inlet with septum head for use with liquid N2 PTV inlet with septumless head for use with liquid CO2 PTV inlet with septum head for use with liquid CO2 PTV inlet with septumless head for use with liquid N2 PTV inlet with septum head for use with liquid N2 Volatiles interface Flip Top Inlet Sealing System
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Agilent GC Detectors
A comprehensive selection of detectors
Flame Ionization Detector (FID)
Designed for maximum sensitivity and ease of use, the most popular and versatile detector Flame that can be ignited from keyboard or automatically Full digital linear dynamic range (107) in a single run
Optimized selectivity and sensitivity for nitrogen- and phosphorus-containing compounds NPD with Blos bead offers robust operation, better stability and longer bead lifetime Bead is digitally set from the keyboard and is auto-correcting
Universal detector responds to all compounds, excluding carrier gas Single-filament, single-column design
Selective for sulfur- or phosphoruscontaining organic compounds Dual wavelength FPD can detect both sulfur and phosphorus in the same run
Micro ECD designed for better linearity, sensitivity and reduced susceptibility to contamination Nickel plating on the inner surface of the lower cell body to reduce adsorption and degradation of sensitive compounds Anode purge to extend cell lifetime
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Agilent GC Detectors
Description Flame Ionization Detector for packed and capillary use Flame Ionization Detector capillary optimized Thermal Conductivity Detector Micro Electron Capture Detector Flame Photometric Detector Dual Wavelength Flame Photometric Detector Nitrogen Phosphorus Detector for packed and capillary use Nitrogen Phosphorus Detector for packed and capillary use with Blos Bead Nitrogen Phosphorus Detector capillary optimized Nitrogen Phosphorus Detector capillary optimized with Blos Bead 7890 Part No. G3461A G3462A G3432A G2397AD G3435A G3436A G3495A G3463A 7890 Option No. G3440A #210 G3440A #211 G3440A #220 G3440A #231 G3440A #240 G3440A #241 G3440A #250 G3440A #252 6890 Part No. G1561A G1562A G1563A G2397A G2333A G2334A G1575A
Superior bead lifetime Faster attainment of stable operation at initial start-up, as well as more stable operation throughout bead's lifetime Superior sensitivity and selectivity for phosphorouscontaining compounds Similar sensitivity and selectivity for nitrogen-containing compounds Superior immunity to moisture
G3496A G3464A
G1576A
All detectors come standard with EPC for precise digital control of carrier and make-up gases for accuracy and reduced gas consumption.
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PPB-level detection limits No hydrocarbon quenching Linear, equimolar response to organic nitrogen compounds; also responds to ammonia, hydrazine, hydrogen cyanide, and NOx Dual Plasma Burner and Controller also provides an embedded nitrosamines option Adapters for simultaneous NCD and FID operation
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Detection limits at ppb-level No hydrocarbon quenching Linear, equimolar response to sulfur compounds ASTM Methods approval Adapters for simultaneous SCD and FID operation
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Improves data quality for better analytical results Reduces cycle time by eliminating long bake-out times for highly-retained compounds Reduces maintenance and lowers operating costs by protecting columns and detectors
Allows safe disconnection of GC column without venting mass spectrometer and without losing vacuum Lets you change columns in about 30 seconds Allows for backflushing
Flow Splitting
Sends sample to multiple detectors to maximize information from a single run Helps locate peaks of interest faster, and achieve better integration of target peaks Use of multiple detectors provides higher confidence when identifying unknowns Allows for backflushing
Deans Switch
Enables two-dimensional GC ("heart-cutting") Allows for lower detection limits of trace compounds in complex matrices Allows for backflushing
G3182-61580 G1580-61060
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Direct heating of a capillary column allows ramp rates of up to 1800C/min (although practical rates depend on column, configuration and desired resolution) Fast cooling times less than one minute for some configurations Excellent retention time repeatability comparable to conventional GC Run up to four column modules simultaneously with different temperature programs to address more difficult analytical challenges Works seamlessly with Agilent Capillary Flow Technology to minimize column maintenance and provide significant new capabilities in multi-dimensional gas chromatography and comprehensive GC
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LTM Systems for Standard Size Column Modules (for fastest cooling)
LTM Systems for Small Size Column Modules (for mounting 3-4 modules)
LTM external power supply LTM transfer line module, small format LTM transfer line module, standard format LTM Module Control Kit, small format LTM Module Control Kit, standard format
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Agilent J&W LTM column modules combine a fused silica capillary column with heating and temperature sensing components wound around it. The design heats and cools the column very efficiently for significantly shorter analytical cycle times compared to conventional air-bath GC oven techniques, while simultaneously using less power.
LTM column modules are available in two column assembly sizes: 5 inch diameter (standard) and 3 inch diameter (small). The standard format provides faster cooling speeds than the small format. Since the chromatography quality is basically equivalent for the two module sizes, Agilent recommends the 5 inch (standard) configuration. For a complete listing of LTM Column Module part numbers, see the 2009-2010 Essential Chromatography and Spectroscopy Catalog, publication number 5989-9611EN.
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Self-aligning "plug and play" injector mounts in seconds without tools; Can be easily moved from one inlet to another, or quickly and easily transferred between GCs when workloads change Greater solvent capacity (>20 mL); Ability to load up to 150 samples allow longer unattended operation Lightweight, removable design permits easy inlet maintenance
Modular design lets the 7693A ALS work seamlessly with all Agilent 7890 and 6890 (optional controller box required) gas chromatographs; the 7693A injection tower is also supported on Agilent 6850 and 7820 systems Easily add features or capabilities as your needs change
Exclusive dual simultaneous injection feature saves time by doubling your sample throughput Proprietary Agilent fast-injection technology 2x faster than any competitive ALS minimizes needle discrimination and sample degradation Next sample overlap capability allows you to significantly decrease turn-around-time by performing pre-run rinses and picking up the next sample before the current run is complete
Programmable injection capability lets you optimize for performance, cost-efficiency or unique research requirements Variable injection parameters: In addition to two standard injection speeds, you can customize all injection parameters including injection speed, syringe plunger speed on draw and injection and needle height into the sample vial and injection port The 7693A system can also perform simple liquid manipulations on the sample prior to injection; for example, a derivatization agent could be added, the sample vial heated, mixed and then injected into the system all automatically
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Available in new 250 L and 500 L volumes, as well as a full suite of volumes from 0.5 L to 100 L Deliver better accuracy via alignment with the Agilent autosampler stroke Provide longer plunger lifetime before failure Show less wear on inlet septa due to a superior needle finish Fully compatible with Agilent's earlier autosamplers Provide excellent results for low carryover between samples Packaged in environmentally friendly, easy-to-open packaging
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70-sample tray 12 positions in the heating oven Multiple headspace-extraction mode Integrated ChemStation control software available
Related Supplies
Agilent offers a wide selection of headspace supplies including certified vials, caps and septa. For more information about Agilent's comprehensive offering of columns and supplies, order the 2009-2010 Essential Chromatography and Spectroscopy Catalog, publication number 5989-9611EN. Visit www.agilent.com/chem/reserve to request your free copy.
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Low-volume injections with minimal needle discrimination and background interference High-volume injections up to 500 L without chromatographic degradation (requires an appropriately configured GC) Headspace vial processing that promotes simple, transparent analysis for ultimate confidence in your results SPME injections that reduce sample preparation time and eliminate the need for large volumes of extraction solvents
CTC PAL injectors work with any Agilent 7890A, 6890, or 6850 GC or GC/MSD system. Specialized software controls are also available for GC ChemStation, MSD Productivity ChemStation, and EZ Chrom. Additionally, CTC PAL sample injectors are designed to expand your labs capabilities with features such as:
A top-mounted design that saves valuable bench space Multiple sample vial and well plate options for greater automation Integrated software for easy setup, control, and sequencing Automated sample preparation and large-volume injection capabilities for greater productivity
Large tray capacity for higher sample throughput and increased periods of unattended operation Temperature-controlled Peltier cooling to prevent sample degradation A variety of CTC PAL-compatible supplies to support your specific applications
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CTC Analytics PAL Autosampler Supplies
To better serve Agilent instrument users who choose CTC Analytics autosamplers for high-throughput and flexible sample introduction, Agilent has partnered with CTC to provide a fully integrated system, including CTC approved consumables. Agilent's portfolio includes a variety of vials, caps, syringes, and well plates compatible with the CTC Analytics HTC PAL, GC PAL, and CombiPAL systems.
Large-volume injection allows you to inject samples up to 500 L without the usual degradation in chromatographic performance Eliminates the need to concentrate samples through evaporation, which can translate into substantial time savings Low-volume injection minimizes needle discrimination and reduces background interference, giving you better results with less work Fast injection cycle time, together with the nanoliter injection mode, fits perfectly into the field of fast GC With either large- or low-volume injection type, control steps including fill/inject speed, pre- and post-injection delay times, and pre- and post-injection cleaning using Agilent's ChemStation software for GC and GC/MSD systems
Simple, straightforward sample analysis No dead volume or adsorption effect in sample No loops and transfer lines The ability to adjust sample volumes without changing sample loops Vial pressurization eliminates the need to dilute the sample
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Thermal Desorption
Purchase Markes International Thermal Desorption products directly from Agilent
Thermal Desorption (TD) allows you to introduce volatile and semi-volatile compounds from a wide range of sample matrices, directly into a GC or GC/MSD. Markes TD products are fully supported by Agilent's sales and service network. For over a decade, Markes International has pioneered and commercialized enhancements to analytical TD instrumentation and associated sampling apparatus. The Markes Series 2 TD platform consists of the UNITY 2, UltrA 2, Air Server 2, and CIA8 products allowing analysis of single tubes, real-time air samples, and canisters with options for automated analysis.
SecureTD-Q for quantitative repeat analysis Electronic RFID tube tagging DiffLok caps for sample integrity Patented inert valving for compound compatibility Electrically-cooled trapping Choice of autosamplers for unattended analysis Wide range of supplies and sampling equipment
Thermal Desorption
Description Markes UNITY Series 2 Thermal Desorber Markes UltrA Series 2 Autosampler, 100 tube capacity Upgrade with 50:50 module for UltrA Series 2 Upgrade with Internal Standard/Dry Purge module for UltrA Series 2 Upgrade with 50:50 and Internal Standard/Dry Purge modules for UltrA Series 2 Markes Air Server Series 2 (3-channel autosampler) Markes CIA8 (8-channel canister interface) Part No. MKI-UNITY2 MKI-ULTRA2 MKI-ULTRA2 MKI-ULTRA2 MKI-ULTRA2 MKIAIRSV MKI-CIA8 Option No.
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Completely portable Interfaced with a laptop, the Micro GC can be carried to any site, anywhere More sensitive In addition to having a TCD that is 10x more sensitive than conventional TCDs, Agilent 3000A Micro GCs now include a performance enhancement (option 001) that allows it to perform trace-level analysis in the low ppm range EPC control Advanced digital pneumatics control enables electronic carrier gas flow, enhancing reliability and precision while further simplifying operation
Easy operation for operators at various skill levels Sample-focused software requires just a few mouse clicks to start the analysis and generate a report Access anywhere LAN connectivity enables remote access to the instrument and data; or you can have a remote instrument or process computer automatically start the GC analysis
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Popular Applications
Natural gas and LPG Refinery gases Landfill gases Fuel cell Mine safety Transformer oil Oil and gas exploration Chemical manufacturing Environmental General purpose fixed gas
Fully customizable Custom configurations suit your application needs with 1 to 4 chromatographic channels. Choose from various micro-machined injectors, columns, sample conditioners, and application-specific reporting Module-level flexibility The modular GC design maximizes uptime, with repair as simple as exchanging one module for another. You can be back up and running in less than an hour Injectors to fit your analysis You can increase sensitivity with the variable volume injection option; maintain high precision and repeatability with the fixed volume injection option; remove unwanted contaminants from your sample or speed up analysis with the backflush injection option
Natural Gas Analyzer A compact, turnkey, easy-to-use system designed for reliability and trouble-free service; The 3000 NGA is supplied complete with instrument, application-specific software, calibration gases, and computer Refinery Gas Analyzer Performs a full-range refinery gas analysis: detailed saturated and olefinic hydrocarbons (C1-C6 and C6+ grouped peaks), fixed gases (H2, O2, N2, CO, CO2), H2S, SO2, and COS analysis in less than 160 seconds
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The Triple-Axis Detector combines a next-generation off-axis design and triple-channel electron multiplier for the industrys lowest detection limits Synchronous SIM/Scan mode lets you selectively monitor for ions of interest at high sensitivity while simultaneously acquiring spectra at scan rates up to 12,500 /s Trace Ion Detection lowers your Method Detection Limit (MDL), as well as your Limit Of Quantitation (LOQ), while reducing false negatives
Deconvolution Reporting Software, together with new Retention Time Locking databases, dramatically reduce false negative results and post-run analysis time Backflush using Capillary Flow Technology reduces the analysis cycle time, minimizes chemical interference from matrix carryover, and extends column life
Advanced instrument control, high productivity data analysis and easily customizable reporting features help you get more work done in less time Agilent's Retention Time Locking (RTL) is now easier to use and ensures reproducible retention times instrumentto-instrument and lab-to-lab anywhere in the world Integration with Agilent OpenLAB Enterprise Content Manager (ECM) streamlines data handling and organization
The inert, ultra-reliable, high temperature ion source maintains optimal peak shape and reduces cleaning requirements, especially when analyzing dirty samples High temperature gold-plated quartz quadrupole with 1050 mass range stays clean even with complex, high boiling samples Simple, automatic tuning takes the trial and error out of system setup, saves time and improves instrument-to-instrument consistency QuickSwap Capillary Flow device lets you safely disconnect the column without venting, and without losing vacuum in about 30 seconds Advanced GC inertness is achieved with Inert MS columns, inlet liners and supplies
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Part No.
Include a 5975C Inert MSD, high-performance MSD ChemStation, LAN hub and cables. Do not include a 7890A, 6890, or 6850 GC. EI bundles do not include an ion gauge or gauge controller. 5975C VL MSD/Data System Bundle Diffusion Pump, EI Source G3241A 5975C Inert MSD/Data System Bundle Standard Turbo, EI Source G3242A 5975C Inert MSD/Data System Bundle Performance Turbo, EI Source G3243A 5975C Inert XL MSD/Data System Bundle Performance Turbo, G3245A EI/PCI/NCI Source, Ion Gauge Controller
GC/MSD Bundle
Includes a 5975C VL MSD, high-performance MSD ChemStation, LAN hub and cables. Does not include an ion gauge or gauge controller. 5975C MSD/6850 GC/Data System Bundle Diffusion Pump, EI Source G3246A
MSD-Ready GC Bundle
7890 GC bundle for 5975 Series GC/MSD, includes split/splitless inlet and MSD interface
A la Carte MSDs
G3442A
Do not include GC, PC, printer, LAN hub, MSD Productivity ChemStation, or ion gauge controller. 5975C VL MSD EI Diffusion Pump G3170A 5975C Inert MSD Standard Turbo EI mainframe G3171A 5975C Inert MSD Performance Turbo EI mainframe G3172A 5975C Inert XL MSD Performance Turbo EI/PCI/NCI mainframe G3174A
Accessories
G3397A G1701EA G1732BA G1710EA G1711EA G1712EA G1717EA G1729EA G1739EA G1740EA G1716AA G3249AA
MSD Productivity ChemStation Software MSD Security ChemStation Software MSD Data Analysis Only Software G1701EA Additional User License G1710EA Additional User License G1710EA Data Analysis PC/Monitor/Printer Bundle Standard MSD ChemStation PC/Monitor/Printer Bundle MSD Productivity ChemStation License/PC/Monitor/Printer Bundle Academic MSD ChemStation PC/Monitor/Printer Bundle Deconvolution Reporting Software DRS Bundle with NIST and RTL Database
*All other GC configurations should be ordered from the GC list with Option 201 for the MSD interface.
For a list of available MS Libraries and RTL Databases, see page 26.
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Part No. G1033A G1035B G1038A G1039D G1041A G1730A G1731B G1671AA G1672AA G1673AA G1674AA G1675AA G1676AA G1677AA G1716AA G3249AA G2923AA G1639AA G3384AA G1617EA G1652BA G1618EA G1692AA G1638AA
NIST 2008 MS Library Bundle Wiley 8th edition with NIST 2008 MS Library Stan Pesticide MS Spectral Library Maurer/Pfleger/Weber 2007 Toxicology MS Library NIST 2008 MS Library Upgrade Wiley 8th/NIST 2008 MS Library Upgrade Maurer/Pfleger/Weber Library 2007 Upgrade
RTL Databases
Hazardous Chemical RTL Database Library Pesticide RTL Database Library Indoor Air Toxics RTL Database Library Forensic Toxicology RTL Database Library JPN Positive List Pesticide RTL Database Library Fiehn GC/MS Metabolomics RTL Database Library Environmental Semi-Voas RTL Database Library
Special Software and Software Upgrades
Deconvolution Reporting Software DRS Bundle with NIST and RTL Database Integrated Headspace Control Software for MSD Productivity ChemStation Headspace Software for MSD Upgrade to A.01.04 CTC PAL Control for MSD ChemStation G1701EA MSD ChemStation Software Upgrade MSD Security ChemStation Software Upgrade G1701EA Data Analysis Only Software Upgrade Pesticide RTL Database Software Upgrade MSD-DRS Software Upgrade
California Department of Food and Agriculture (CDFA) Number of pesticide hits Number of false positives Time required to process 37
Extend the qualitative and quantitative power of your MSD with Deconvolution Reporting Software (DRS)
NIST AMDIS (Automated Mass Spectral Deconvolution and Identification Software) is now fully integrated with ChemStation QEdit and reporting. It is easier than ever to extract trace analytes from complex matrices and deconvolute coeluting peaks for high quality library searches against Retention Time Locked databases and the NIST library.
8 hours
32 minutes
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Part No. G3392A G3393A G3397A G3398A G3399A G3185B G2591A G2860B G1086A G1088A G1088B G2591A G2860B 59864B
Triple Axis Detector Upgrade Kit PCI/NCI Upgrade for 5975 Series MSD Ion Gauge Controller Dry Pump Upgrade Kit NH3 Dry Pump Upgrade Kit MSD QuickSwap Inert Ion Source Assembly Inert 5973/75 Extended Linearity App Kit
Upgrades for the 5973 Series MSD
PCI/NCI Upgrade for 5973 MSD 5973A Performance Electronics Upgrade 5973N/Inert Performance Electronics Upgrade Inert Ion Source Assembly Inert 5973/75 Extended Linearity App Kit Ion Gauge Controller
Fast column removal or replacement without the need to cool and vent the MSD Risk-free inlet and column maintenance by preventing air from entering the MSD Short analysis times and increased sample throughput by removal of high molecular weight compounds using backflushing mode
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Routine femtogram-level sensitivity and superior selectivity in complex matrices Next-generation off-axis, triple-channel detector minimizes noise and maximizes signal for the lowest detection limits
Outstanding data acquisition speed up to 500 MRM transitions per second matches the front-end performance of the fastest chromatography without compromising data quality enabling you to automatically quantify and confirm more targets in a single method MassHunter software provides powerful, easy-to-use data analysis; Review and reporting tools let you process more samples in less time with complete confidence in your results
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Inert, ultra-reliable ion source Agilent's unique, dual-filament ion source design increases productivity and reduces cleaning requirements, especially when analyzing dirty samples High temperature, gold-plated quartz quadrupole with 1050 mass range stays clean even with complex, high boiling samples eliminating frequent retuning, time-consuming maintenance and ensuring superior mass analyzer performance
Proprietary collision cell technology with patent-pending helium quench gas contributes to outstanding high-speed performance without ion ghosting or cross-talk Simple, automatic tuning takes the trial and error out of system setup, saves time and improves instrument-toinstrument consistency
G3440A G7011A
#994
7890 GC bundle for 5975 Series GC/MSD Includes Split/Splitless Inlet and MSD Interface
Upgrades for 7000A
G3442A
G7004A
All other GC configurations should be ordered from the GC list with Option 201 for the MSD interface.
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Option No.
G4513A G4349A
G4350 #203 G4350 #44N G4350 #44P G4350 #161 G4350 #152 G4350 #210 G4350 #220 G4350 #235 G4350 #250
Split/Splitless Capillary injection port Packed Column injection port Flame Ionization Detector (FID) Thermal Conductivity Detector (TCD) Micro Electron Capture Detector (ECD)** Nitrogen Phosphorus Detector (NPD)
*The 7820A Gas Chromatograph can support a maximum of 2 inlets, 2 detectors and an auxillary heated zone. Optional items include a 16 sample automated liquid sampler, valving, EzChrom Elite Compact Software, and installation/familiarization. **Not available in Japan
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Infinite power range that combines ultra high pressure up to 1200 bar and high flow rates
up to 5 mL/min for maximum chromatographic performance, compatibility, flexibility and investment protection New 1290 Infinity Binary Pump combines active damping combined with lowest delay volumes facilitated by proprietary multi-layer technology in the new Agilent Jet Weaver mixer to provide ultra fast gradients and superior LC/UV and LC/MS performance New optical design in 1290 Infinity Diode Array Detector including Agilent Max-Light cartridge flow cell offers a new level of UV-sensitivity and baseline robustness, including fast spectral acquisition at data rates up to 160 Hz Next generation flow-through design of the 1290 Infinity Autosampler achieves highest precision for both large and small injection volumes without changing sample loops New 1290 Infinity Flexible Cube combines with Autosampler to facilitate fixed-loop injection mode for ultra fast cycle times, or automatic backflushing of needle seat for a new benchmark in lowest carryover New Agilent Quick-Change valves in the 1290 Infinity Thermostatted Column Compartment boosts usability and paves the way for ultrahigh throughput, multi-method and automated method development solutions
The power range of the Agilent 1290 Infinity LC is the widest available, combining ultrahigh pressures up to 1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance, compatibility, flexibility and investment protection.
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High resolution chromatography 90,000 plates in 4 minutes Full compatibility with existing HPLC methods More detection capabilities from UV-Visible and ELSD through LC/MS Near-zero sample carryover for uncompromised data quality High system flexibility for automated method development
Maximize your performance by combining the Agilent 1200 Series Rapid Resolution System with Agilent ZORBAX Rapid Resolution HT columns. ZORBAX RRHT columns use a totally porous, 1.8 m particle to provide maximum resolution in fast, ultra-fast and high resolution analyses.
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Entry-level system for world-class LC technology Easy and fast maintenance Flow rates up to 10 mL/min for a wide range of column dimensions and applications Upgrade options from isocratic up to automated quaternary LC system
High pressure gradient mixing for superior gradient performance even at lower flow rates Optimized delay volumes for fast chromatography Flow rates from 0.05 to 5 mL/min provide ideal support for narrow and standard bore applications
Greatest flexibility for multi-solvent gradients by accessing up to 4 solvents Flow rates up to 10 mL/min support narrow bore, standard and semipreparative applications Wide selection of autosamplers for full flexibility in injection volumes and sample containers
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Related Literature
Order the complete 1200 Series brochure from your local Agilent representative or Agilent Authorized Distributor using publication number 5989-5200EN.
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Analytical scale covering flow rates up to 10 mL/min Preparative scale covering flow rates up to 100 mL/min Automated delay volume calibration
Integrated safety features Software solutions for different user needs Smart fraction collection based on time, peak or mass
Part No. G1322A G1310A G1354A G1312A G1329A G2260A G2258A 5065-9922 G1316A G1364C G1330B G1160A G1361A G1391A G2260A G2258A 5065-9922 G1316A G1383A G1364B G1330B G1160A
1200 Series Vacuum Degasser 1200 Series Isocratic Pump 1200 Series Quaternary Pump 1200 Series Binary Pump 1200 Series Standard Autosampler 1200 Series Preparative Autosampler 1200 Series Dual-Loop Autosampler Manual prep injection valve kit, stainless steel 1200 Series Thermostatted Column Compartment 1200 Series Fraction Collector AS 1200 Series Thermostat for Autosamplers (2x) 1200 Series 12-Position/13-Port Valve
Preparative Scale Purification
1200 Series Preparative Pump 1200 Series Preparative Pump Gradient Extension 1200 Series Preparative Autosampler 1200 Series Dual-Loop Autosampler Manual prep injection valve kit, stainless steel 1200 Series Thermostatted Column Compartment 1200 Series Column Organizer 1200 Series Fraction Collector PS 1200 Series Thermostat for Autosamplers (2x) 1200 Series 12-Position/13-Port Valve
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Part No. G1314B G1315D G1365D G1365B G1365B G1365B G1315D G1365D
Option No.
1200 Series Variable Wavelength Detector 1200 Series Diode Array Detector 1200 Series Multiple Wavelength Detector
Preparative Scale Flow Cells for Multiple Wavelength Detector
Stainless steel flow cell, 3 mm path length Quartz flow cell, 0.3 mm path length Quartz flow cell, 0.06 mm path length
Analytical Scale Flow Cells for Multiple Wavelength Detector
Related Literature
Order the primer Principles of Preparative HPLC from your local Agilent representative or Agilent Authorized Distributor using publication number 5989-6639EN.
Semi-micro flow cell, 6 mm path length, 5 L volume, pressure tight to 120 bar Standard flow cell, 10 mm path length, 13 L volume, pressure tight to 120 bar
Preparative Scale Flow Cells for Diode Array Detector
Stainless steel flow cell, 3 mm path length Quartz flow cell, 0.3 mm path length Quartz flow cell, 0.06 mm path length
Analytical Scale Flow Cells for Diode Array Detector
Semi-micro flow cell, 6 mm path length, 5 L volume, pressure tight to 120 bar Standard flow cell, 10 mm path length, 13 L volume, pressure tight to 120 bar
Analytical Scale Flow Cells for Variable Wavelength Detector
Semi-micro flow cell, 6 mm path length, 5 L volume, pressure tight to 40 bar 1200 Series Fluorescence Detector 1200 Series Refractive Index Detector 1200 Series Evaporative Light Scattering Detector
Mass-Based Fraction Collection
#016
Mass-Based Fraction Collection Kit 1200 Series ESI LC/MSD VL system bundle 1200 Series ESI LC/MSD SL system bundle
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More than 50 LC modules for different detection modes and different budgets Automated switching among up to eight columns and up to 15 different solvent pairs Up to 288 different test conditions for binary gradient separation No need to disconnect columns or exchange solvent bottles
Choose from different application software packages Agilent ChemStation Method Scouting Wizard Agilent Easy Access for walk-up systems supporting different columns Agilent partner solutions for complete automated method optimization
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Ordering Information
Many configurations of pumps, autosamplers and detectors are possible. A working system requires at least two thermostatted column compartments (both including Option #058) as well as one Valve Kit for 400 bar (G4230A) or 600 bar (G4230B) operation. For system control, Agilent ChemStation revision B.04.01 or higher is required. Below is a typical high-end configuration for up to 600 bar operation with column and solvent selection, and with MS detection.
Part No. G1312B G1312B G1312B G1160A 5067-1567 G1379B 5065-9981 5067-4601 G1367D G1315C G6140AA G1316C G1316C G4230B G4230B G4230B G4230B G2196AA
Option No.
1200 Series Binary Pump SL Solvent Selection Valve Active Seal Wash
Solvent Selection
#031 #030
1200 Series 12-Position/13-Port Valve Cover Kit for Binary Pump 1200 Series Micro Degasser Solvent Cabinet Solvent Selection Tubing Kit, 4 solvents
Injection
1200 Series Diode Array Detector SL 6140 Single Quadrupole LC/MS System
Column Selection
1290 Infinity Thermostatted Column Compartment Integrated Valve Drive High-Pressure Method Development Valve Kit Capillary Kit for Short, Low-Dispersion Column Column Kit, RRHT 2.1 mm selectivity range Column Kit, RRHT 2.1 mm pH range
Software
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Up to 500 times more sensitive compared to conventional LC Typical flow rates 1-100 L/min, extendable up to 2.5 mL/min Advanced diode array detection from 190-950 nm
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Up to 3500 times more sensitive compared to conventional LC Typical flow rates 0.1-1 L/min, extendable up to 2.5 mL/min Compatibility with third-party MS platforms
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Active flow control for exceptional flow stability Flexibility of collection in well-plates, Eppendorf tubes and MALDI targets Liquid control for highest reproducible collection of small volumes Fraction cooling prevents evaporation and thermal decomposition
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Up to 3500 times more sensitive compared to conventional LC No peak dispersion for uncompromised chromatographic performance Sample preparation and separation columns, connection capillaries, fittings, and nanospray emitter integrated directly on the polymer chip
MS Calibration and Diagnostic Chip For infusion of MS Tuning Mix and calibration of the Chip Cube Microvalve Protein ID Chip #1 For peptide separations, low/medium complexity tryptic digest mixtures. 100-400 ng on-column. 40 nL enrichment column, 75 m x 43 mm analytical column. Stationary phase: 5 mm, C-18 SB-ZORBAX, 300. Protein ID Chip #2 For peptide separation, medium/high complexity tryptic digest mixtures. Up to 1 g on-column. 40 nL enrichment column, 75 m x 150 mm analytical column. Stationary phase: 5 mm, C-18 SB-Zorbax, 300.
G4240-61001 G4240-62001
G4240-62002
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Glycan Chip For Glycan (oligosaccharides) separations. Can be used for highly polar compounds and structurally related compounds such as geometric isomers and diastereoisomers. 40 nL enrichment column, 75 m x 43 mm analytical column. Stationary phase: 5 mm, Graphitized Carbon. Small Molecule Chip For small molecules retained and well characterized on SB-C18 stationary phase. 40 nL enrichment column, 75 m x 43 mm analytical column. Stationary phase: 5 mm, C-18 SB-ZORBAX 80. Infusion Chip Direct infusion or automated flow injection of the sample directly into the mass spectrometer at nanoflow rates for MS and MS/MS data collection. Custom Chip For customers requiring non-standard stationary phase material and/or non-standard chip layout and functionality. Minimum order of 5 chips. Requires Special Product Quote (SPQ).
Predesigned Custom HPLC Chips
G4240-65001
G4240-61002
G4240-63001
These HPLC-Chips are not available from stock and are made to order. Delivery time is 4 weeks after acceptance of order. Intact Protein Chip For separation of intact proteins up to 80 kDa. 40 nL enrichment column, 75 m x 43 mm analytical column. Stationary phase: 5 mm, C-8 SB-ZORBAX, 300. Direct Injection Chip For direct injection of the sample onto the separation columns without enrichment. On-chip 16 nL injection loop, 75 m x 150mm analytical column. Stationary phase: 5 mm, C-18 SB-ZORBAX, 300. Protein ID Chip #3 High capacity enrichment column. For peptide separation, high complexity tryptic digest mixtures. Up to 4 g on-column. 160 nL enrichment column, 75 m x 150 mm analytical column. Stationary phase: 5 mm, C-18 SB-ZORBAX, 300. UHC Small Molecule Chip For small molecule separations requiring high capacity and wide polarity range coverage. 500 nL enrichment column, 75 m x 150 mm analytical column. Stationary phase: 5 mm, C-18 SB-ZORBAX, 80. The use of an in-line filter is strongly recommended when using this chip for biofluid analysis.
G4240-63001 SPQ105
G4240-63001 SPQ100
G4240-63001 SPQ110
G4240-63001 SPQ115
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Ideal for small- to medium-sized labs using standard HPLC for routine analysis
Easy to learn and use for all users Extraordinary results in a system optimized for everyday productivity Industry-leading quality and long-life Agilent reliability Five standard configurations to fit your LC workflow A complete, integrated software solution, scalable from a single user to an entire enterprise Agilent columns and supplies for an extra measure of reliability and confidence Agilent's customer-enabled service and support features for maximized uptime
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G4287AA
G4288AA
G4289AA
G4290AA
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6110 Economical entry to the power of LC/MS 6120 Adds the productivity of ion polarity switching 6130 Research-grade LC/MS with faster scanning and outstanding sensitivity 6140 Maximum performance plus ultra-fast scanning for fast chromatography and high-throughput applications
Faster acquisition speeds to take full advantage of high-throughput separations such as RRLC Ultra-fast ion polarity switching (up to 20 spectra/second) provides maximum information from a single injection Faster injection-to-injection cycle time lets you run more samples per hour
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Sub-1 ppm typical mass accuracy improves confidence and reduces false positives Data acquisition rates up to 40 spectra per second assure maximum data quality and compatibility with fast chromatography Up to five orders of in-spectrum dynamic range reveal trace-level targets even in the presence of vastly more abundant compounds Low picogram on-column sensitivity finds impurities or biomarkers at extremely low concentrations Exceptionally accurate intact protein MW determination allows rapid QC of recombinant therapeutics The 6230 Accurate-Mass TOF LC/MS with Agilent Jet Stream thermal gradient focusing provides a 5- to 10-fold improvement in MS and MS/MS sensitivity at optimal LC flow rates, for even greater analytical confidence MassHunter Workstation software's fully automated data analysis routines completely exploit the high information content of accurate-mass MS data from Agilent TOF LC/MS systems
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Whether you are searching for low abundance proteins, drug metabolites in complex matrices, pesticide residues in food products, or confirming compound identity, there is a 6300 Series Ion Trap LC/MS System to meet your needs.
6310 Makes high performance MSn economical 6320 Provides enhanced sensitivity and faster scan speed 6330 Offers ultimate sensitivity for low-abundance analytes 6340 Improves PTM characterization and protein identification
Low femtogram sensitivity and fast scan speeds enhance compound identification in complex samples Automated, data-dependent MS/MS and MSn maximize the amount of high-quality data from a single run, increasing the number of compounds identified Optional Electron Transfer Dissociation (ETD) improves peptide fragmentation and allows Post Translational Modifications (PTMs) such as phosphorylation to be more easily identified and located
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Femtogram sensitivity, regardless of application Maximized ion generation and transmission across a broad mass range ensure low limits of detection and quantitation for the widest range of sample types Fast, sensitive MRMs An innovative collision cell design eliminates cross-talk and enables the analysis of large multianalyte panels, such as pesticides in food or targeted protein quantitation Automated method development and optimization software selects the best possible conditions to maximize sensitivity for each compound The MassHunter acquisition software features comprehensive data-dependent and targeted MS/MS capabilities, as well as automated MRM method development software (MassHunter Optimizer), scheduled MRM and 21 CFR Part 11 compliance support for the 6400 Series Triple Quadrupole LC/MS
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Sub-1 ppm MS and 2-4 ppm MS/MS mass accuracy increases confidence in small molecule ID and reduces false positive rates in protein database searches Superior attomole-to-low-femtomole sensitivity helps you identify even very low abundance compounds Fast data acquisition rates of up to 20 spectra/second ensure maximum compatibility with fast LC and high-throughput methods Broad mass range of 25-20,000 m/z enables detection of small molecules, peptides or intact proteins Agilent Jet Stream technology is available on the 6530 Accurate-Mass Q-TOF LC/MS to provide maximum sensitivity for multiple applications, including the analysis of drug candidates and trace levels of food contaminants, metabolites or biomarkers MassHunter Workstation softwares fully automated data analysis routines completely exploit the high information content of accurate-mass MS and MS/MS data from Agilent Q-TOF LC/MS Systems
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Patented orthogonal nebulization simplifies operation and reduces maintenance Heated counterflow drying gas improves performance Dual nebulizer maximizes mass accuracy
Agilent Multimode Source True simultaneous ESI & APCI to maximize coverage and sample throughput
The Agilent Multimode Source is a true breakthrough in LC/MS ionization the only ion source capable of simultaneously generating ions by Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI).
Description Multimode Source 6000 Series Part No. G1978B Older MS Series Part No. G1978A
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Popular Applications
Designed for nanoelectrospray LC/MS applications such as protein identification, intact protein, glycoprotein and phosphoprotein analysis, and small molecule applications for pharmaceutical development. By incorporating many components of a traditional nanoflow LC/MS system into a biocompatible polymer chip, HPLC-Chip technology delivers uncompromising separations and dramatically increases LC/MS sensitivity.
Work with complex mixtures, analyze limited sample amounts and detect subtle yet significant changes with confidence Quickly and easily switch between different methodologies without the challenges of replumbing a nanoflow LC system HPLC-Chips are available for proteomics and metabolomics applications, small molecule separations, flow-injection or direct infusion, and customization with new layouts, features and functionality
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The following are special nebulizers that can be added to the standard Electrospray Source:
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Enables highly sensitive MS analysis of pesticides No matrix effects in the analysis of drugs in biological fluids
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pI-based OFFGEL fractionation with 0.1-0.6 pH resolution depending on consumable kit used In-solution fraction recovery for easy transfer to LC/MS Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration Up to 16 samples fractionated at the same time g to mg load capacity Possible to run conventional in-gel IEF as well as OFFGEL fractionation Preconfigured methods for OFFGEL and in-gel modes Online current control for each individual sample
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5188-6444
5188-6425
5188-6424
5188-6427
5188-6426
5188-6428
5188-8013
For more information about the Agilent 3100 OFFGEL Fractionator, visit www.agilent.com/chem/offgel
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Performs in a laminar flow hood Pipetting range of 100 nL to 200 L Serial dilutions with standard pipette heads Interchangeable pipette heads are swappable in minutes User configurable positions for filtration, temperature control, shaking and more
Related Supplies
Agilent offers a wide selection of liquid handling and microplate handling consumables, such as disposable tips and thermal microplate seal and adhesive labels for barcoding. For more information, visit www.agilent.com/lifesciences/automation.
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Unique eight plate position, small footprint design conserves space for all applications Seamless integration into a variety of Agilent and third party automation systems Simultaneously pipette while other plates are changed for impressive throughput and significant reduction in assay time Pipetting range of 100 nL to 200 L provides savings on costly reagents and compounds so you can miniaturize your assays with confidence
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High speed, eight second transfer times from stack to instrument 2, 4, or 6 stack configurations for a maximum of 360 standard microplates Storage and handling of most microplates, lidded plates, tip boxes and tube racks 66% more walk-away time than competitive systems Integration of multiple instruments into a single benchtop workstation
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Related Literature
Request the PlateLoc Thermal Microplate Seal Selection Guide, publication number 5990-3659EN, from your local Agilent representative or Agilent Authorized Distributor.
Touch screen interface for fast and easy manual operation Automatically adjusting to accommodate a wide range of microplates and tube racks, the PlateLoc can handle all of your sealing needs High precision, with sealing temperature control of 2C and advanced seal slitting control, seal integrity will be the same for every plate Fast machine cycle times, and no required cool down periods mean you will spend your time sealing plates, not waiting With the industry's smallest instrument footprint and numerous proven integrations, the PlateLoc is an ideal choice for system integrators
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Compact footprint, saving valuable lab space Expand capacity and functionality in the future; just add another BioCel System Increased flexibility and capacity with under-table incubators or microplate carousels QuickDock docking tables available to easily swap devices or use instruments offline Inert, Class 100, and humidity-controlled environments available VWorks software, an event-driven controller, ensures highest instrument utilization and maximum system productivity
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Push button operation for manual use or automated operation via host PC Applies labels on any permutation of one, two, three, or four sides of a microplate in one of two vertical positions Wide range of fonts, barcode formats and magnifications for maximum flexibility; Barcode symbologies include Code 39, Interleaved 2 of 5, Code 128 and many others Repeatedly print and apply labels at speeds of up to 1 every 3 seconds Verify bar codes with available barcode reader and reapply labels if necessary
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Product Specifications
Accessories
Wavelength range 190 to 1100 nm Acquisition speed down to 0.1 s Stray light of less than 0.03%
PC controller with ChemStation software Multi-cell transport Peltier thermostatted cell holder Sipper system Autosamplers
Agilent high-precision cells are tested to meet the highest optical standards. Every Agilent cell comes with a test certificate, ensuring that the following areas have been tested and are within specifications:
Homogeneity of the raw material Dimensional and angle tolerances of the component parts
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Product Specifications
Particle size accuracy of 0.3% Concentration accuracy better than 10% Small particle sensitivity of 5% Particle size measurement in 5 seconds
With Agilent's 7010 Particle Size Analyzer, you don't have to sacrifice simplicity or resolution to measure complex dispersions.
Concentration (%) 0.000005 83
This graph shows the particle size analysis of a mixture of 25% 80 m gold standard and 75% 140 m gold standard.
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Zeta potential No dilution required Automatic particle size corrections Built-in potentiometric and volumetric titration Software wizards
Titania: Uncoated vs Coated 60.00 Zeta Potential [mV] 30.00 0.00 -30.00 -60.00 -90.00 Uncoated Silica Coat
In this example, the IEP of the uncoated sample is 6.2, while that of the silica coated sample is below pH 1.
0.00
2.00
4.00 pH
6.00
8.00
10.00
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Large dynamic range 0.5 nm to 6 m particle sizes High resolution able to separate close bi-modal distributions Low volume samples < 10 mL Absolute measurement calibration
REL. 100 80 60
Using its proprietary deconvolution algorithm, Agilent's 7030 Nicomp can uncover true bi-modal distributions. In this example, the main peak was detected at 1.5 nm, while a secondary peak of aggregates was detected at 17 nm.
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Particle size range 0.5 to 2500 microns Particle sensitivity of 10 PPT Particle size accuracy of 2% Particle count accuracy of 10%
Multiple configurations for best fit to application All systems come with software and PC controller Automated operation for reliable results New Focused Extinction (FX) technique for high concentrations Customized process (on-line) versions available
The 7080 AccuSizer, with its ability to count and size individual particles, is a sensitive tool for constructing a material's true particle size. Here, five latex standards of varying size were used in a single sample; all were successfully located by Agilent's 7080 AccuSizer.
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Detector sensitivity
30% lighter with a 25% smaller footprint Modular architecture enables easy
servicing & maintenance
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G7100B
G1602A G1603A G1607A 5063-6514 5063-6520 5064-8228 5064-8208 5064-8206 5063-6510 5063-6511
#021
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One platform endless possibilities for DNA, RNA, protein and cell analysis
The Agilent 2100 Bioanalyzer is the industry standard for RNA sample QC and has replaced labor-intensive gel electrophoresis for this application. It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples. A unique feature of the Agilent 2100 Bioanalyzer is that it can be used for both electrophoretic separation and flow cytometric analysis of cell fluorescence parameters. This versatility makes the Agilent 2100 Bioanalyzer an indispensable tool for the molecular biologist and biochemist.
Ready-to-use assays and pre-packaged reagent kits for DNA, RNA, proteins and cells Minimal sample consumption (1-5 L) and results within 30 minutes Replaceable electrode cartridge for contamination-free switch of methods Digital data for convenient analysis, archiving and storage share data with others and export it for publication or presentation Various data-display options as gel view, electropherograms and tables Easy to use with simplified sample comparison Minimum exposure to hazardous materials Supports compliance with 21 CFR Part 11
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Cell Solutions
On-chip flow cytometry an easy way to acquire cell-based fluorescence parameters
The flow cytometry set for the Agilent 2100 Bioanalyzer allows scientists working with cells to perform simple flow cytometry assays. The set extends the lab-on-a-chip application portfolio of the Agilent 2100 Bioanalyzer from electrophoretic separation assays to automated two color flow cytrometric assays. In brief, six samples each 10 L with 20,000 prestained cells are loaded onto the chip and the fluorescence intensities in two channels for about 750 single cells per sample are measured within 25 minutes. Please note: cell solutions are not suitable for the electrophoresis-only Bioanalyzer (G2939AA).
Easy to use, short setup time with no adjustment of complex instrument parameters Analysis of a wide range of cell fluorescence parameters Predefined flow cytometric assays for easy startup or protocol development for extra flexibility Low cell consumption (20,000 down to 2,500) enables flow cytometry analysis of primary and other precious cells On-chip staining procedure speeds up workflow Agilent Cell Checkout Kit checks proper instrument and cell assay operation Agilent Cell Kit for analysis of cell fluorescence parameters
Cell Solutions
Description Cell Kit for analysis of cell fluorescence parameters Cell Checkout Kit Part No. 5067-1519 5067-1520
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DNA Solutions
A smarter solution for nucleic acid analysis
The Agilent 2100 Bioanalyzer with its DNA kits allow you to automatically size and quantitate PCR fragments and restriction digests accurately and reproducibly. PCR and RT-PCR are among the most widely used techniques in molecular biology. While in some cases, it is sufficient to detect the presence or absence of a PCR product, in many cases quantitation of this product and detection of unspecific amplification is critical. The Agilent 2100 Bioanalyzer is the tool of choice for automated sizing and quantitation of PCR and RT-PCR products in single or multiplex mode with unprecedented accuracy. With the advent of Next-Generation Sequencing (NGS) platforms; sizing, quantification and quality assessment of fragmented DNA starting material, as well as DNA sequencing libraries, have become essential to achieve highest quality sequencing data. The new Agilent High Sensitivity DNA Kit allows the sizing and quantification of DNA samples in the single-digit pg/L concentration range.
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Increased sensitivity for DNA fragment analysis down to 5 pg/L Sizing, quantification and quality control of complex DNA samples down to 100 pg/L
for fragmented DNA or DNA sequencing libraries
Sizing accuracy Normalization to two internal markers and a ladder Quantitation accuracy and reproducibility Automated quantitation of each DNA fragment
against internal standards High resolution of a large number of bands Critical for any multiplex PCR application Broad linear dynamic range Enables the detection of less abundant products, e.g. low abundance messages in multiplex RT-PCR amplifications or non-specific amplifications Sensitivity LIF detection for fragments down to 0.1 ng Minimal sample consumption Only 1 L of material required per analysis Quick and easy sample comparison One-click overlay, scaling or zooming features Improved assay precision Pre-packaged reagents and standardized assay protocols yield highly reproducible data
DNA Solutions
Description Agilent High Sensitivity DNA Kit for Next-Gen Sequencing workflows Agilent DNA 1000 Kit for high resolution PCR fragment analysis Agilent DNA 1000 Reagents Agilent DNA 7500 Kit for impurity analysis of PCR products Agilent DNA 7500 Reagents Agilent DNA 12000 Kit for impurity analysis of PCR products Agilent DNA 12000 Reagents Part No. 5067-4626 5067-1504 5067-1505 5067-1506 5067-1507 5067-1508 5067-1509
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Protein Solutions
The fast and reliable way to replace analytical SDS-PAGE methods
The Agilent Protein 230 and Protein 80 Kits provide a fast and easy way to analyze a wide range of samples, using Lab-on-a-Chip technology. When used with the Agilent 2100 Bioanalyzer, each chip is capable of providing size, purity and concentration information for ten different protein samples within 30 minutes. The Lab-on-a-Chip approach eliminates handling SDS-PAGE slab gels, staining or imaging steps. The Agilent High Sensitivity Protein 250 kit is the first microfluidic assay for protein detection in the low picogram range. It offers four orders of magnitude linear dynamic range, allowing quantification of low concentrated impurities next to dominant main peaks in a single run.
Fast results Complete automated analysis of 10 samples in 30 minutes No manual staining and destaining steps required All procedures automated during chip run Improved assay precision Pre-packed reagents and standardized assay protocols yield highly reproducible data High Sensitivity Protein 250 kit provides silver stain sensitivity (1 pg/L labeled BSA on Chip) and four orders of magnitude linear range in quantification Analyze a large variety of protein samples Cell lysates, column fractions, antibodies and purified proteins Minimal sample consumption Only 4-5 L of protein sample required per analysis Quick and easy sample comparison One-click overlay, scaling or zooming features Sizing and quantitation in one assay Allows for relative and absolute quantitation
Protein Solutions
Description Agilent Protein 80 Kit Agilent Protein 80 Reagents (no chips included) Agilent Protein 230 Kit Agilent Protein 230 Reagents (no chips included) Agilent High Sensitivity Protein 250 Kit Agilent High Sensitivity Protein 250 Reagents (no chips included) For separation Agilent High Sensitivity Protein 250 Reagents (no chips included) For labeling reaction Agilent High Sensitivity Protein 250 Ladder Part No. 5067-1515 5067-1516 5067-1517 5067-1518 5067-1575 5067-1576 5067-1577 5067-1578
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RNA Solutions
Fast quality control of RNA with minimal sample consumption
RNA quality is critical to the success of microarray and qPCR experiments. The Agilent 2100 Bioanalyzer with its RNA kit portfolio is the industry standard for RNA quality control. It allows monitoring the quality of RNA samples and reliably identifying degraded RNA samples. The software embedded RIN algorithm automates RNA integrity assessment and provides user-independent data.
RNA 6000 Nano Kit assess the integrity of total and miRNA samples RNA 6000 Pico Kit measure RNA samples in the pg/L range Small RNA Kit detect and visualize miRNA content in total RNA
RNA Solutions
Description Agilent RNA 6000 Nano Kit Agilent RNA 6000 Nano Reagents Agilent RNA 6000 Nano Ladder Agilent RNA 6000 Pico Kit Agilent RNA 6000 Pico Reagents Agilent RNA 6000 Pico Ladder Agilent 2100 Small RNA Kit Agilent Small RNA Reagents Agilent Small RNA Ladder Part No. 5067-1511 5067-1512 5067-1529 5067-1513 5067-1514 5067-1535 5067-1548 5067-1549 5067-1550
Related Information
Order your free copy of the new Agilent 2100 Bioanalyzer RNA Integrity CD at www.agilent.com/chem/rna-cd.
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Reduces the plasma potential and narrows the ion energy spread, enabling optimum
interference removal in the ORS cell by energy discrimination On the 7700s, the ShieldTorch also provides complete flexibility for every application by delivering unmatched cool plasma performance for ultimate BECs in simple matrices where required
Simpler, more reproducible plasma optimization with one-click plasma setting Expert Auto Tuning the fastest, most effective and most reliable system optimization
program ever developed for ICP-MS One-touch access to the interface area, and easier sampling cone removal/refitting during routine maintenance
MassHunter Workstation
Outlier flagging of values such as count RSD, ISTD recovery, calibration linearity, calibration
range, etc. ensures potential analytical problems can be located immediately Graphical display of internal standard recovery, QC stability plots, calibration curves and mass spectrum (or chromatogram) Flexible reporting export raw data and processed results for single samples, user-defined selections, or an entire batch to Microsoft Excel or your LIMS
Optional Software
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Works effectively using helium (He) mode, for simplified operation and consistent results,
even in complex sample matrices
He does not react with sample matrix, analyte, or internal standard elements, and therefore
cannot form new interferences
Higher sample throughput with a single gas mode, as no time is lost switching
between gases
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Agilent ASX-520 Autosampler is ideal for medium to high sample throughput applications,
with rack configurations providing up to 360 vial positions Integrated Sample Introduction System with Discrete Sampling (ISIS-DS) delivers reduced matrix loading and improved productivity for very high throughput applications Pre-configured LC-ICP-MS kits are available for turn-key methods, such as separation of As species in urine and water GC-ICP-MS interface for separation and detection of volatile species
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Enhanced performance in He mode with the ORS3 cell that provides up to 10x lower
LODs compared to the 7500cs Reaction mode using hydrogen cell gas as standard, and optional 3rd cell gas line for other reaction gases, such as NH3 or O2 Built-in tuning and method templates for all common semiconductor applications, including collision/reaction mode and cool plasma mode, ensure the highest data quality and lowest BECs Lower heat output and reduced exhaust vent flow, with a redesigned cooling air-flow management system particularly important in clean-room installations
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Agilent Integrated Autosampler (I-AS) a covered autosampler with pumped rinse station,
ideal for ultra-trace analysis and small sample volumes (as low as 0.5 mL); flexible rack configurations offer a maximum capacity of 89 vials, plus 3 rinse vials Inert Sample Introduction kit suitable for high-purity reagents O-ring-free and manufactured from PFA for the lowest contamination levels and HF resistance Organics kit contains the sample introduction parts you need to run volatile organic solvents
Unrivaled Support
With approximately 500 ICP-MS systems installed in semiconductor laboratories around the world, Agilent has an extensive and unrivaled support structure in place for semiconductor ICP-MS users. Worldwide support is available for hardware, software or application questions, delivered by experienced engineers. In addition, Agilent users have access to methods or Standard Operating Procedures (SOP) for all common semiconductor matrices to simplify your method development.
Platinum interface cones as standard Optimized exhaust system (single vent, low pressure-drop) for ducting in cleanroom use
Agilent 7700s ICP-MS Typical Configuration
Description 7700s ICP-MS Mainframe MassHunter Workstation ICP-MS Checkout Solutions Water Recirculator Integrated Autosampler (I-AS) with Type E Tray Cell Gas Purification Kit PFA Inert Sample Introduction Kit Part No. G3282A G7210B 5185-5850 G1879B G3160B G3269A G4912A Option No.
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Workflow Solutions
Gene Expression Explore gene transcription on a genome-wide basis across a variety of model systems Comparative Genomic Hybridization Conduct high-resolution, genome-wide profiling of DNA copy number changes from most sample types, including FFPE tissue MicroRNA Profile miRNAs and explore the role played in gene regulation with the most robust experimental protocols and frequent array design updates Chromatin Immunoprecipitation Elucidate the role that protein-DNA interactions play in processes such as transcription, replication, modification and repair DNA Methylation Create high-resolution, genome-wide methylation profiles and gain insight into mammalian DNA methylation and gene regulation Genome Partitioning Focus next-gen sequencing efforts on key genomic regions of interest, enabling more powerful and cost-efficient studies of genetic diversity
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Part No. G2565CA G2539A G2530-60029 G2534A G2534-60003 G2534-60002 G2534-60011 G2534-60014
Hybridization oven rotator rack Hybridization chamber, stainless steel Backing slides, 1 array/slide 5 slides/kit* Backing slides, 2 arrays/slide 5 slides/kit* Backing slides, 4 arrays/slide 5 slides/kit* Backing slides, 8 arrays/slide 5 slides/kit*
*Larger quantities available
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User-changeable thermal blocks for 96-well, 96-well fast, and 384-well operation Preloaded, preprogrammed user protocols for Stratagene-brand PCR enzymes Gradient operation with temperature range of 30C-99C and up to 30C gradient span
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Description Mx3005P QPCR System 110V with laptop PC Mx3005P QPCR System 110V with desktop PC Mx3005P QPCR System 230V with laptop PC Mx3005P QPCR System 230V with desktop PC Mx3000/Mx3005P install/validation plate, B-actin 96-well QPCR plates, non-skirted 96-well QPCR plates, semi-skirted Mx3000P/Mx3005P 96-well plate tubes Mx3000P/Mx3005P strip tubes Single PCR reaction tubes (without caps) Mx3000P/Mx3005P optical strip caps Benchtop rack for 200 L tubes/V bottom plates Mx3000P/Mx3005P replacement bulb assembly One year pre-purchased Mx3000P/Mx3005P service contract One year Mx3000P/Mx3005P service contract
Part No. 401449 401456 401457 401458 600567 401333 401334 410088 401428 410023 401425 410094 401411 401463 401464
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Sensitive QPCR and QRT-PCR using SYBR Green and Probe-Based Detection
The next generation Stratagene Brilliant II QPCR and QRT-PCR Master Mix Kits offer superior sensitivity for improved quantification and reproducibility. The New Brilliant II Kits also show more consistent performance across a wide range of template concentrations and different targets. Our Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
Brilliant II SYBR Green QPCR Master Mix Delivers Better Sensitivity and Reproducibility Across a Wide Range of Concentrations
Standard curve plot of tenfold dilutions detecting a GAPDH target (150 bp, 52% G/C) from 100 ng down to 0.01 ng of cDNA. The Brilliant II SYBR Green QPCR Master Mix detects template at ~ 1-3 Cts earlier than competitor reagents and with perfect efficiency and Rsq values.
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Detects mature miRNA Differentiate miRNA that differ by a single nucleotide Sensitive detection down to 10 copies miRNA specific forward primers
Wide, Linear, Dynamic Range of at Least Seven Logs Allows Quantitation of a Few Copies to Millions of Copies
37 36 35 34 33 32 31
Log fit values SYBR6 Standards, RSq:0.998 SYBR6, Y= -3.395*LOG(X) + 25.79, Eff. = 98.5% SYBR3 Standards, RSq:0.996 SYBR3, Y= -3.354*LOG(X) + 23.13, Eff. = 98.7% SYBR4 Standards, RSq:0.997 SYBR4, Y= -3.240*LOG(X) + 27.55, Eff. = 103.5%
let-7c miR-23a
let-7i
Ct (dRn)
30 29 28 27 26 25 24 23 22 21 20
miR-21 let-7a
0.1 1
SYBR1 Standards, RSq:0.997 SYBR1, Y= -3.318*LOG(X) + 30.06, Eff. = 100.2% SYBR8 Standards, RSq:0.999 SYBR8, Y= -3.252*LOG(X) + 25.48, Eff. = 103.0%
Linearity was demonstrated with twofold dilutions of HeLa cDNA and detection of five different miRNAs of varying abundance; miR-21, miR-23a, let-7a, let-7c, and let-7i. This wide range in signal linearity allows for accurate quantitation of miRNAs of varying abundance.
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User-changeable thermal blocks for 96-well, 96-well fast, and 384-well operation Preloaded, preprogrammed user protocols for Stratagene-brand PCR enzymes Gradient operation with temperature range of 30C-99C and up to 30C gradient span
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Description Mx3005P QPCR System 110V with laptop PC Mx3005P QPCR System 110V with desktop PC Mx3005P QPCR System 230V with laptop PC Mx3005P QPCR System 230V with desktop PC Mx3000/Mx3005P install/validation plate, B-actin 96-well QPCR plates, non-skirted 96-well QPCR plates, semi-skirted Mx3000P/Mx3005P 96-well plate tubes Mx3000P/Mx3005P strip tubes Single PCR reaction tubes (without caps) Mx3000P/Mx3005P optical strip caps Benchtop rack for 200 L tubes/V bottom plates Mx3000P/Mx3005P replacement bulb assembly One year pre-purchased Mx3000P/Mx3005P service contract One year Mx3000P/Mx3005P service contract
Part No. 401449 401456 401457 401458 600567 401333 401334 410088 401428 410023 401425 410094 401411 401463 401464
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Sensitive QPCR and QRT-PCR using SYBR Green and Probe-Based Detection
The next generation Stratagene Brilliant II QPCR and QRT-PCR Master Mix Kits offer superior sensitivity for improved quantification and reproducibility. The New Brilliant II Kits also show more consistent performance across a wide range of template concentrations and different targets. Our Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
Brilliant II SYBR Green QPCR Master Mix Delivers Better Sensitivity and Reproducibility Across a Wide Range of Concentrations
Standard curve plot of tenfold dilutions detecting a GAPDH target (150 bp, 52% G/C) from 100 ng down to 0.01 ng of cDNA. The Brilliant II SYBR Green QPCR Master Mix detects template at ~ 1-3 Cts earlier than competitor reagents and with perfect efficiency and Rsq values.
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Detects mature miRNA Differentiate miRNA that differ by a single nucleotide Sensitive detection down to 10 copies miRNA specific forward primers
Wide, Linear, Dynamic Range of at Least Seven Logs Allows Quantitation of a Few Copies to Millions of Copies
37 36 35 34 33 32 31
Log fit values SYBR6 Standards, RSq:0.998 SYBR6, Y= -3.395*LOG(X) + 25.79, Eff. = 98.5% SYBR3 Standards, RSq:0.996 SYBR3, Y= -3.354*LOG(X) + 23.13, Eff. = 98.7% SYBR4 Standards, RSq:0.997 SYBR4, Y= -3.240*LOG(X) + 27.55, Eff. = 103.5%
let-7c miR-23a
let-7i
Ct (dRn)
30 29 28 27 26 25 24 23 22 21 20
miR-21 let-7a
0.1 1
SYBR1 Standards, RSq:0.997 SYBR1, Y= -3.318*LOG(X) + 30.06, Eff. = 100.2% SYBR8 Standards, RSq:0.999 SYBR8, Y= -3.252*LOG(X) + 25.48, Eff. = 103.0%
Linearity was demonstrated with twofold dilutions of HeLa cDNA and detection of five different miRNAs of varying abundance; miR-21, miR-23a, let-7a, let-7c, and let-7i. This wide range in signal linearity allows for accurate quantitation of miRNAs of varying abundance.
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Laboratory Workflows
Analytical Biology Chemistry
LIMS Bioinformatics Partnering with LIMS vendors ECM API SDK GeneSpring DNA Analytics eArray
Chromatography/Instrumentation/Spectrometry
ChemStation
MassHunter
EZChrom Elite
Feature Extraction
OpenLAB ICM
OpenLAB ECM
I&F
Customization
IQ/OQ
SMA
Consulting
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Agilent ChemStation
Highest Level of Control for Agilent Instrumentation
Agilent ChemStation is the industry leading chromatography data system for Agilent instrumentation, handling the widest variety of separation techniques such as GC, LC, LC/MS, CE and CE/MS. It is a scalable data system ideally suited for applications in all industries ranging from early product development to quality control. Extensive customization capabilities as well as configurable regulatory compliance provide the flexibility to support different workflows. Sophisticated level-5 control and monitoring of LAN-based instruments ensures fast and flexible data acquisition, which is complemented by advanced data analysis and reporting capabilities for highest productivity.
ChemStation integration with OpenLAB Enterprise Content Manager (ECM) enables data storage and management in one central repository.
Control of Agilent LC, GC, CE, CE/MS and LC/MS instruments and generic A/D converters as well as detectors such as ESA Biosciences' Coulochem and Corona CAD detectors Advanced, easy to use data review capabilities for fast result generation and sample-based reporting Add-on software modules for support of specific R&D workflow solutions
Enhanced integration with OpenLAB Enterprise Content Manager (ECM) provides for central management of data, master methods and sequence templates More than 40 ChemStation user privileges can be combined into different roles and administered in OpenLAB ECM Seamless integration in non-regulated or highly-regulated workflows, including support for 21 CFR Part 11 (requires OpenLAB ECM)
Efficient and flexible processing and presentation of sequence-based results or across sequences with the add-on OpenLAB Intelligence Reporter Easily customization through built-in macro language to meet individual needs Seamless compatibility of data, method and sequence files with earlier revisions Backwards compatibility for control of older GPIB-based instrumentation
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EZChrom Elite with Agilent 1100/1200 control, DAD, Smart Sequence and Run Queue
Software that is scalable from a Single User to multi-site Client/Server Enterprise Work smarter and be more productive with Smart Sequencing Powerful, Flexible Reporting Tools Meet the needs of GLP and 21 CFR Part 11 with Configurable Compliance Tailored to the needs of petrochemical and pharmaceutical laboratories Single interface throughout the lab simplifies operation and user training Controls the 1120 LC and 7820 GC
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Fast, easy setup and operation with automated tuning for Agilent GC/MS single quadrupole instruments Control of two GC/MSD systems from a single PC Simultaneous acquisition of SIM and Scan data for high sensitivity quantitation and library searchable spectra Simultaneously acquired MSD and GC detector signals
Import/export of shared methods (eMethods) Guided setup of new calibration tables based upon automatic integration and library search results (AutoQuant) Automatic conversion of any full scan method to a high sensitivity SIM or SIM/Scan method (AutoSIM)
General purpose and tailored report packages: Enhanced, EnviroQuant (USEPA), DrugQuant and Aromatics in Gasoline (ASTM) Macro programs to automate repetitive steps (mouse actions, menu choices and typed entries) and customize processes MSD Security ChemStation to address data security, integrity and traceability mandated by FDAs 21 CFR Part 11
Quantitative power for over 2000 compounds at 20 levels of calibration with 4 curve fit options Reprocess a previously run sequence while acquiring data Direct comparison of multiple data files from multiple detectors (MS and GC)
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Agilent OpenLAB
A New Operating System for the Laboratory
OpenLAB is the world's first operating system for the analytical laboratory. Based on Agilent's strategy to provide an open architecture, OpenLAB can deliver every laboratory the means to effectively manage its instruments, workflows, and information created on end user desktops into a highly secure and scalable content management system. OpenLAB integrates analytical instrument control and advanced data analysis, enterprise content management and laboratory business process management into a single, scalable web based system.
Intelligent indexing of scientific data Powerful tools to search across all file types Automated data transfer to ECM with configurable delete policies Full suite of compliance tools to ensure data security and integrity Seamless integration with MS Office Scalable from a single PC to a multi-server, multi-site enterprise
OpenLAB ECM uses a simple Explorer-based user interface, reducing training and familiarization. ECM information is organized in folders with view/read/write privileges for security.
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OpenLAB ICM offers full-featured chromatography workflow support. The ICM main page displays all relevant run information and status, with further review just a click away.
An add-on application to Enterprise Content Manager (ECM) Instrument data and information is stored in ECM and includes full searching and sorting, on-line archiving, maintenance operations, and more When coupled with the Business Process Manager (BPM), instrument maintenance schedules can be automated and instruments can be locked down from use when maintenance is not performed in a timely manner
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Creating workflows is simple with the MS Visio based business process manager. This example workflow reflects a specification acceptance procedure that could be implemented for a product's release.
Streamline, automate, and optimize mission-critical business processes while enabling seamless collaboration between people, processes and content Designed with ease-of-use in mind, so there is zero coding required to define, configure, and deploy a business process User interfaces are based on familiar productivity tools that every knowledge worker understands, such as Microsoft Internet Explorer, Visio, Outlook, Excel, and Adobe Designer (for eForms)
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An add-on application to Enterprise Content Manager (ECM) Increase efficiency of data review by interactive reporting Faster decisions by content-based result presentation
OpenLAB Intelligence Reporter's interactive reporting and content-based results provide impurity-profiling reports with complex calculations and fields to enter impurity levels for flagging of outliers.
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Agilent Kalabie
Spend Less Time on Paperwork and More Time on R&D
If creating and managing knowledge is a critical part of your organizations mission, here's how to do it faster, easier and smarter: Kalabie Electronic Lab Notebook (ELN). More than just "going paperless," Kalabie ELN gives you a scalable, integrated platform for cross-team collaboration. In an increasingly complex global environment, it simplifies and accelerates the R&D process and streamlines data management all within a robust IP protection solution. Built around an open architecture, Kalabie ELN is a multi-discipline solution for chemistry, analytical and biology laboratories and has flexible tools to design workflows that reflect your lab's processes.
Chemistry interface for chemical synthesis. Kalabie ELN accommodates chemistry cartridges from a variety of suppliers. Planned experiments are designed in the user interface and then can be easily captured in a PDF format to quickly create reports.
Kalabie ELN has an experiment management interface available for users to manage and store experiment parameters. Information can be recalled quickly with the search interface, allowing users to create, execute and store complex queries combining many criteria into a Boolean expression. The Signature Workflow feature is imprinted into each notebook file and stored with each experiment's results. The Analytical Request Manager (ARM) provides the necessary link between chemical product development and analysis laboratories; Scientists can submit requests for sample analysis directly from the Kalabie interface.
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Fast, easy setup and operation with automated tuning for both triple quadrupole and Q-TOF instruments and importing worklists from spreadsheets to save time in method Powerful yet simple data analysis with separate, specialized modules for intrument control and data acquisition, qualitative identification of unknowns, and quantitation of target compounds Fast, flexible data reporting with preconfigured reports that simplify basic analyses, or completely customized reports courtesy of Microsoft Excel and XML
Archive MassHunter data, results and methods in Agilent OpenLAB Enterprise Content Manager
For archiving MassHunter data files, including results and methods, a seamless integration with Agilent OpenLAB Enterprise Content Manager (ECM) enables easy indexing and searching of all sample information with automatic scheduling of data transfer to an ECM server.
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Powerful tools help you extract the information you're looking for
Unlike traditional data processing software that works on 2D chromatograms (a), MassHunter Workstation software uses its unique Molecular Feature Extractor (MFE) algorithm to work directly with the 3D accurate mass LC/MS data set (b), MFE fully and automatically removes noise due to matrix contribution (c) and extracts compound chromatograms (d) and compound mass spectra for each component. The overlaid compound chromatograms (e) show the exact location and elution profile for each compound in the sample, even if multiple compounds partially or fully co-elute.
a)
b)
c)
d)
e)
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MassHunter Quantitative Analysis software ensures that you spend less time analyzing your data. It includes helpful features such as batch-at-a-glance data review, a curve-fit assistant, dynamically linked results, outlier flagging and customizable views. All this, with the flexibility of compound- or sample-centric navigation.
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Fragments table
MassHunter Metabolite ID software simplifies metabolite identification without taking away your choice or control. The versatile, powerful application package can reduce your data processing, review and reporting from days to hours.
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MassHunter Mass Profiler software includes easy-to-use statistical and visualization tools that allow the identification of statistically meaningful differences between two sample groups.
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GeneSpring MS Software
Agilent's GeneSpring MS software is a powerful solution for the discovery of biomarkers through the analysis of mass spectrometric data. You can easily import, normalize, compare, and display mass spectral data from large sample sets and complex experimental designs. This includes GC/MS data, and LC/MS or CE/MS data from Agilent TOF, Q-TOF, and Triple Quadrupole instruments.
GeneSpring MS is a unified, easy-to-use platform for mass spectral data normalization and comparison that helps you answer the complex biological questions targeted by metabolomics and proteomics studies.
Interactive Sequence Editor/Matcher in the BioConfirm software helps locate the site of the modification, when analysis of recombinant proteins indicates that the wrong protein was produced. The unique protein Molecule Feature Extractor enables complex mixture analysis via related ion deconvolution.
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Spectrum Mill for MassHunter Workstation accurately determines not only protein identities but also relative abundances of a given protein in multiple samples. Color coding makes relative abundances apparent at a glance.
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Agilent GeneSpring
The Gold Standard for Desktop Expression Analysis
GeneSpring provides powerful, accessible statistical tools for fast visualization and analysis of gene expression data. Regarded as the gold standard in desktop expression analysis, GeneSpring allows you to quickly and reliably identify targets of interest that are both statistically and biologically meaningful.
The GeneSpring user interface showing flexibility available for calculating and displaying analytical results.
Identify differentially expressed microRNAs and use TargetScan information to identify their gene targets, analyze real-time PCR data, and build relevant biological interaction networks from a database of gene product interactions provided in GeneSpring; Detect significant differences in alternative splicing events. Guided workflows step you through a typical analysis with limited decision points while advanced workflows allow expert users to access all GeneSpring tools and define parameters and cut-offs for each analysis. GeneSpring provides a suite of statistical tools including paired and unpaired t-tests, 1-way and multi-way ANOVA, Repeated Measures ANOVA, and the permutative method of p-value calculation.
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Consulting Services
Application Consulting Identification of customer objectives and goals; Strategic recommendations for complementing people, process and technology components Software Enablement and Integration New and existing product installation/upgrade, configuration, testing and optimization Workflow Design Create tailored processes and procedures leveraging industry best practices Validation and Compliance Streamlined qualification services to ensure compliance with industry and regulatory standards Custom Projects Special, customer-specific projects which fall outside the scope of the offerings described above; May include custom applications development, integration work or project management oversight
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Consulting Services
Application Consulting
Custom Projects
Bronze *
Standard
Advanced
Custom
For more information on Agilent Support and Services for Instrument Systems, see the following pages.
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Agilent Advantage Gold Priority-one coverage for ultimate uptime and productivity Agilent Advantage Silver Comprehensive coverage for dependable laboratory operations Agilent Advantage Bronze Total repair coverage at a fixed annual price Agilent Repair Service Basic coverage for reliable instrument repair
Agilent Advantage service plans include Agilent Remote Advisor for real-time remote monitoring and diagnostics. Through secure internet connections, you can interact with Agilent service professionals, receive detailed asset reports, and configure text or email alerts to notify you before problems occur helping you to maximize instrument uptime and optimize laboratory workflows
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Improve qualification efficiency by automating protocols across platforms to ensure greater efficiency and minimize regulatory risk Standardize your entire compliance operation with robust test designs that work with all your instruments Add, remove or reconfigure tests based upon your unique user requirements Significantly reduce staff review time with consistently formatted, computer generated, tamper-proof reports
Classroom and on-site training in instrument operation, troubleshooting and maintenance Customized consulting services to meet your labs unique needs
For more detailed information, please go to www.agilent.com/chem/services or contact your local Agilent Services and Support representative.
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Sample Preparation
SampliQ High-Performance Solid Phase Extraction (SPE)
Impurities in samples can contaminate results. Agilent SampliQ SPE will help you confidently extract and concentrate samples from complex matrices so all your analyses are more accurate and reliable. SampliQ sorbents and cartridges are made with the same exacting quality you've come to expect from Agilent instruments and columns. Now you can get that superior performance at the very beginning of your analysis. SampliQ SPE products:
Offer Polymer, silica and other sorbents to meet every SPE requirement Help ensure reproducible results Provide cleaner, concentrated samples
Visit www.agilent.com/chem/SampliQ for more information, part numbers and application notes.
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Columns
Agilent columns are designed and manufactured to offer excellent and reproducible performance.
Agilent J&W GC Columns
As the worlds leading provider of GC capillary columns, Agilent is uniquely positioned to offer you superior quality and unmatched service and support. Every column is individually tested to meet the tightest quality control specifications in the industry. We have the widest range of GC/MS and Ultra Inert stationary phases proven to deliver consistent column inertness and exceptionally low column bleed with high upper temperature limits.
For more information about Agilent's comprehensive offering of columns and supplies, order the 2009-2010 Essential Chromatography and Spectroscopy Catalog, publication number 5989-9611EN. Visit www.agilent.com/chem/reserve to request your free copy.
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Notes
Agilent e-Seminars
The latest technology and newest developments are just a click away with free e-Seminars from Agilent
With different series and topics for troubleshooting, maintenance, informatics, compliance, and industry applications, Agilent e-Seminars are the easy way to get the latest information without the hassle or expense of travel. These free 60 to 90 minute online seminars are offered continuously:
Choose from live or recorded events to best fit your schedule Participate wherever you are from the office, home, or hotel Ask Agilent experts and industry leaders questions during live Q&A sessions
For more information and to see a complete listing of available sessions, visit www.agilent.com/chem/eseminars You can also register to receive schedules and course information at www.agilent.com/chem/registration
Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies, Inc. 2009 Printed in the USA March 6, 2009 5990-3699EN