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H50 Service Manual - V1.0 - EN

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0% found this document useful (0 votes)

238 views156 pages

H50 Service Manual - V1.0 - EN

h50

Uploaded by

Moises

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H50/H50P AUTOMATED GLYCOHEMOGLOBIN

ANALYZER

SERVICE MANUAL
Table of Contents
Chapter 1 Mechanical System ........................................................................................... 1-1
1.1 Mechanical System Composition ............................................................................... 1-1
1.2 Replacing the FRU...................................................................................................... 1-5
1.2.1 Sample Collection Assembly ........................................................................ 1-5
1.2.2 CT Probe Wipe ............................................................................................. 1-9
1.2.3 Piercing Probe .............................................................................................. 1-9
1.2.4 Mix bath Assembly ..................................................................................... 1-10
1.2.5 Syringe Assembly ....................................................................................... 1-11
1.2.6 Auto Mix Assembly ..................................................................................... 1-12
1.2.7 Spring Tube Pincher ................................................................................... 1-13
1.2.8 Plunger Pump Assembly ............................................................................ 1-15
1.2.9 Plunger Pump ............................................................................................. 1-15
1.2.10 Waste Pump Assembly .............................................................................. 1-17
1.2.11 Degasser Assembly.................................................................................... 1-18
1.2.12 Pulse Damper ............................................................................................. 1-19
1.2.13 Rotation Feeding Valve .............................................................................. 1-21
1.2.14 Cisterns ...................................................................................................... 1-22
1.2.15 Autoloader .................................................................................................. 1-23
1.2.16 Sample Compartment Assembly ................................................................ 1-26
1.2.17 Unload Unit ................................................................................................. 1-28
1.2.18 Temperature Control Assembly .................................................................. 1-29
1.2.19 Filter Assembly ........................................................................................... 1-30
1.2.20 Top Left Cover Assembly (H50) ................................................................. 1-30
1.2.21 Touchscreen ............................................................................................... 1-31
Chapter 2 Fluidic System ................................................................................................... 2-1
2.1 Introduction ................................................................................................................. 2-1
2.1.1 Sample Preparation Channel ....................................................................... 2-1
2.1.2 Chromatographic Channel ........................................................................... 2-2
2.1.3 Temperature Control Unit ............................................................................. 2-3
2.1.4 Reagent Management .................................................................................. 2-4
2.2 Reagent System ......................................................................................................... 2-5
2.3 Fluidic Components and Functions ............................................................................ 2-6
2.3.1 Degasser ...................................................................................................... 2-6
2.3.2 Plunger Pump ............................................................................................... 2-7
2.3.3 Pulse Damper ............................................................................................... 2-7
2.3.4 Feeding Valve/Rotation Valve ...................................................................... 2-8
2.3.5 Analytical Column......................................................................................... 2-9
2.3.6 Filter and Filter Element ............................................................................. 2-10
2.3.7 Back Pressure Sensor ............................................................................... 2-10
2.3.8 Temperature Control of the Analytical Column .......................................... 2-11
2.3.9 Temperature Control of the Optical System ............................................... 2-12
2.3.10 Other Fluidic Components ......................................................................... 2-13
2.4 Fluidic Sequences..................................................................................................... 2-21
2.4.1 Startup Sequence....................................................................................... 2-21
2.4.2 Whole Blood Analysis Mode....................................................................... 2-23
2.4.3 Predilute Analysis Mode ............................................................................. 2-25
2.4.4 Probe Cleanser Maintenance Sequence ................................................... 2-26
2.5 Fluidic Diagrams ......................................................................................................... A-1
2.6 List of Tubes and Connectors ..................................................................................... B-1
Chapter 3 Optical System ................................................................................................... 3-1
3.1 Overview of Optical System Principle......................................................................... 3-1
3.2 Optical System Operating Principle ............................................................................ 3-1
3.3 Optical System Composition....................................................................................... 3-2
3.4 Optical System Status Determination ......................................................................... 3-2
3.5 Maintenance and Replacement of the Optical System .............................................. 3-3
3.6 Optical System Status Verification after Maintenance or Replacement ..................... 3-5
Chapter 4 Hardware System .............................................................................................. 4-1
4.1 Structure of Hardware System.................................................................................... 4-1
4.2 Hardware System Signal Flow Chart.......................................................................... 4-3
4.3 List of Hardware Major PCBA and Cables ................................................................. 4-3
4.3.1 Components of main boards ........................................................................ 4-3
4.3.2 Cables .......................................................................................................... 4-4
4.4 Distribution Diagram of Power Supplies ..................................................................... 4-5
4.5 Introduction to the Hardware Boards .......................................................................... 4-6
4.5.1 Main Control Board ...................................................................................... 4-6
4.5.2 Analog Drive Board .................................................................................... 4-10
4.5.3 Autoloader Board ....................................................................................... 4-15
4.5.4 Preamplification Board ............................................................................... 4-19
4.5.5 LED Drive Board ........................................................................................ 4-22
4.5.6 Liquid Pressure Detection Board ............................................................... 4-25
4.5.7 Touch Screen Control Board ...................................................................... 4-27
4.5.8 Indicator Board ........................................................................................... 4-28
4.5.9 Power Patching Board ............................................................................... 4-30
4.5.10 RFID Card Reader ..................................................................................... 4-32

3
4.5.11 Display Screen Assembly........................................................................... 4-32
4.5.12 Key Board ................................................................................................... 4-33
4.5.13 Power Supply Assembly ............................................................................. 4-34
4.6 Service Instructions for Other Parts Closely-Related To Hardwares ....................... 4-35
4.6.1 Sensors ...................................................................................................... 4-35
4.6.2 Hydraulic Sensor ........................................................................................ 4-36
4.6.3 Temperature Sensors ................................................................................. 4-36
4.6.4 Degasser .................................................................................................... 4-37
4.6.5 Rotary Feeding Valve ................................................................................. 4-37
Chapter 5 Software.............................................................................................................. 5-1
5.1 Introduction ................................................................................................................. 5-1
5.2 Password .................................................................................................................... 5-1
5.3 Functions of Service Access Level ............................................................................. 5-5
5.3.1 Menu Structure Overview ............................................................................. 5-5
5.3.2 Repeatability test .......................................................................................... 5-6
5.3.3 Carryover test ............................................................................................... 5-8
5.3.4 Fluidic Volume Calibration............................................................................ 5-9
5.3.5 Temperature Calibration ............................................................................... 5-9
5.3.6 Pressure Calibration ................................................................................... 5-10
5.3.7 Advanced Setup ......................................................................................... 5-11
5.3.8 Empty/Prime ............................................................................................... 5-12
5.3.9 Self-Test...................................................................................................... 5-13
5.3.10 HPLC Self-Test ........................................................................................... 5-14
5.3.11 Debug->Sample Probe............................................................................... 5-15
5.3.12 Debug-> Barcode Scanner......................................................................... 5-16
5.3.13 Adjustment->Export Debug Data ............................................................... 5-17
5.3.14 Status ......................................................................................................... 5-18
5.3.15 View vulnerable parts info. ......................................................................... 5-19
5.4 Analyzer Update ....................................................................................................... 5-20
5.4.1 Notes before Update .................................................................................. 5-20
5.4.2 Update Steps .............................................................................................. 5-21
5.5 LIS Setup .................................................................................................................. 5-26
5.6 Error Code................................................................................................................. 5-26

4
Chapter 1 Mechanical System

1.1 Mechanical System Composition

On the front of the analyzer, you will find the autoloader, the screen, buttons, etc., inside the
autoloader; there is the sample compartment assembly. Open the left cover, you will find reagent
bags.

Figure 1-1 Front of the analyzer

No. Name No. Name

1 Touchscreen 5 Autoloader
2 Button 6 Sample compartment
3 Tube 7 Left cover (with reagent bags in it)
4 Tube rack 8 Power Switch

1-1
Mechanical System

On the back of the analyzer, you will find the AC input, waste outlet, USB and network interfaces.

Figure 1-2 Back of the analyzer

No. Name No. Name
1 AC input 4 USB interface 1
2 Waste sensor connector 5 USB interface 2
3 Waste outlet 6 Network interface

1-2
Mechanical System

Open the front cover of the analyzer, you will find the sample collection assembly, rotation valve
module, analytical column module, plunger pump module and autoloading module. Open the left
cover, you will find the reagent module.

Figure 1-3 Front of the analyzer (without front cover)

No. Name No. Name
1 Rotation valve module 4 Sample collection assembly
2 Column module 5 Autoloading module
3 Plunger pump 6 Reagent module

1-3
Mechanical System

Open the right cover, you will find the optical module, syringe module, bath assembly, waste pump
assembly, degasser assembly and mix assembly. The optical module is in the upper part of the
analyzer and the bath assembly is inside the right cover.

Figure 1-4 Right side of the analyzer (right door open)

No. Name No. Name
1 Optical module 4 Waste pump assembly
2 Syringe module 5 Degasser assembly
3 Bath assembly 6 Mixing assembly

1-4
Mechanical System

Open the back door of the analyzer, you will find the drive board PCB, autoloader board and the
main control board.

Figure 1-5 Back of the analyzer (back door open)

No. Name No. Name

1 Drive board PCB 3 Main control board
2 Autoloader board

1.2 Replacing the FRU

1.2.1 Sample Collection Assembly

 Tools
NO.107 cross-head screwdriver
 Removal
1） Remove the right door;
2） Revolve to open the top left cover assembly.
3） Remove the 2 M4X8 screws that fix the bath integrating assembly and the front cover, revolve
and open the bath integrating assembly.
4） Take off the line connectors at the end of the drag chain.
5） Move the Y movement assembly horizontally to the center to reveal the 4 M4X8screws.
6） Remove the 4 M4*8 screws.
7） Move the assembly horizontally until it is away from the pinholes, and then take it out from the
opening of the front cover.

1-5
Mechanical System

8） Replace the sampling assembly and align the pinholes, and then mount the 4 M4*8 screws.

No. Name No. Name

1 Top left cover assembly 4 Bath integrating assembly
2 The M4X8 screw of the bath assembly 5 Sample collection assembly
3 The M4X8 screw of the sample collection
assembly

Debug: See the autoloader debug procedure for details.

Structure of the sample collection assembly

The sample collection assembly aspirates, dispenses and mixes sample, and drives the sample
probe to move on two directions.

Figure 1-6 Structure of the sample collection assembly

The X mechanism consists of the horizontal bracket, X guide rod, guide rod clamp, synchronous belt

1-6
Mechanical System

and belt pulley, X initial-position sensor and motor. There are detecting notches in the horizontal
bracket, which correspond to the X direction sensors for position detection.

Figure 1-7 X mechanism of the sample collection assembly

1-7
Mechanical System

Figure 1-8 Y mechanism of the sample collection assembly

The sample probe is driven by the pierce slide; rotation motion of the motor is transferred to
rectilinear motion of the pierce slide through the driving of the screw-nut pairs, and the pierce slide
then drives the Y direction motion of the sample probe, directed by the guiding shaft. The Y initial
position sensor is installed in the pierce bracket; the baffle structure of the pierce slide serves as
sensor baffle to realize Y direction positioning of the sample probe.

1-8
Mechanical System

1.2.2 CT Probe Wipe

 Tools
Tweezers
 Removal
1） Rise the piercing probe to the top until the probe wipe is almost away from the sample
probe.
2） Remove the circlip above the probe wipe manually.
3） Move the probe wipe down to take it off the sample probe.
4） Take off the waste and diluent pipes from the pipe fixing seat, and pull out the pipes co
nnecting to the probe wipe with tweezers.
5） Replace the CT probe wipe, connect the waste and diluent pipes and install them back to the
pipe fixing seat, and then mount the probe wipe back to the piercing bracket and install the
probe wipe circlip.

No. Name No. Name

1 Piercing probe 4 Piercing bracket
2 CT probe wipe 5 Pipe fixing seat
3 Probe wipe circlip

1.2.3 Piercing Probe

 Tools
NO.107 cross-head screwdriver
 Removal
1） Rise the piercing probe to the top until the probe wipe is almost away from the sample probe.
2） Remove the circlip above the probe wipe manually, and move the probe wipe down to take it
off the sample probe.
3） Remove the M3*8 screws in the fixing plate of the sample probe and take of the fixing plate.
4） Take out the piercing probe from the opening.

1-9
Mechanical System

5） Replace the piercing probe, put the probe through the hole of the bracket, make sure the flat
part of the probe is align with the plane of the piercing slide, and then install the fixing plate
back.
6） Install the CT probe wipe and its circlip back.

1 Top side M3 screw 3 Spring tube pincher(5380)
2 Front side M3 screw

 Validation
1） Enter the "Service"→"Debug"→"Autoloader" screen. Put a tube rack onto the autoloader, tap
"Init" and "X Load" in turn to push the tube rack forward, and tap "Y Feed" 3 times to send the
second tube position of the rack to the pinch position of the manipulator.
2） Select "Mix Mechanism", and then tap "Init→X Forward". The manipulator moves out to be on
top of the tube rack. Check visually that the pincher is aligned with the center of the tube
position.

1-14
Mechanical System

1.2.8 Plunger Pump Assembly

 Tools
NO.107 cross-head screwdriver
 Procedure
1） Right revolve to open the top right cover assembly.
2） Remove the 2 M4*8 screws in the bottom right cover with NO.107 cross-head screwdriver to
take off the bottom right cover.
3） Remove the 2 M3 screws by the front side of the plunger pump assembly with NO.107
cross-head screwdriver, and pull the assembly forward to take it off.
4） Replace and install a new plunger pump assembly.

No. Name No. Name

1 Top right cover assembly 3 M4*8 screw
2 Plunger pump assembly 4 Bottom right cover assembly

1.2.9 Plunger Pump

 Tools
NO.107 cross-head screwdriver
 Procedure
1) Take out the plunger pump assembly.
2) Loosen the 8 M3*8 screws under the plunger pump assembly with NO.107 cross-head
screwdriver, and take off the plunger pumps.
3) Replace the plunger pumps and install the assembly back.
 Fluidic volume setup

1-15
Mechanical System

1） Enter the software screen, tap “Service→Fluidic Volume” to enter the plunger pump fluidic volume
calibration screen.
2） Enter the “Flow Rate by Step” and the “Return Difference” for both plunger pumps of Eluent A and
Eluent B.

 Prime
1) After replacement, enter the software screen, tap “Setup→Empty/Prime” to prime the tubes.
2) If pump A was replaced, tap “Eluent A Channel” to perform the priming; if pump B was replaced,
tap ”Eluent B Channel” to perform the priming.

No. Name No. Name

1 Top right cover assembly 3 M4*8 screw
2 Plunger pump assembly 4 Bottom right cover assembly

1-16
Mechanical System

1.2.10 Waste Pump Assembly

No. Name No. Name

1 M4 screw 2 Waste pump assembly

No. Name No. Name

1 M4 screw 2 The entire FRU

The waste pump assembly is consisted of the following 3 FRUs, which shall be replaced together.

1-17
Mechanical System

3001-10-07252 Rotation diaphragm pump

047-012739-00 Pump assembly label (P1)
3101-20-68592 Pump connecting line

1.2.11 Degasser Assembly

No. Name No. Name

1 M4 screw 2 Degasser assembly

 Tools
NO.107 cross-head screwdriver
 Procedure
1) Take off the degasser assembly.
2) Remove the 2 M3 screws that fix the degasser cover with NO.107 cross-head screwdriver and
take the cover off.
3) Loosen the 2 M3*8 screws under the degasser with NO.107 screwdriver.
4) Remove the degasser.
 Prime
1) After replacement, enter the software screen, tap “Setup→Empty/Prime” to prime the tubes.

1-18
Mechanical System

2) Tap “Eluent A Channel” and “Eluent B Channel” to perform the priming.

082-001943-00 Degasser, 2 channels, 285ul/channel
009-004752-00 Degasser connecting line

No. Name No. Name

1 Degasser pinboard 4 M3 screw
2 M3*8 screw 5 Degasser cover
3 Degasser

1.2.12 Pulse Damper

 Tools
NO.107 cross-head screwdriver
 Procedure
1) Remove the right door.
2) Remove the 2 M4 screws that fix the bath integrating assembly, revolve and open the bath
integrating assembly.
3) Open the top right cover.
4) Remove the 2 M3*8 screws at the back of the pulse damper with the NO.107 cross-hea
d screwdriver.
5) Take out the pulse damper from the front.

1-19
Mechanical System

No. Name No. Name

1 Front of the pulse damper 3 M3 screw
2 Top right cover 4 Back of the pulse damper

 Pressure setup
1) On the software, tap “Service→Pressure” to enter the column pressure calibration screen.
2) Enter the pressure value displayed on the pulse damper to the edit box of “K”.
3) Before you perform this procedure, make sure there is no residue pressure from previous
pressure build-up procedure.

1-20
Mechanical System

Enter the pressure value

displayed on the pulse
damper to the edit box
of “K”.
Then adjust the value of
b to make sure the
“ Column Pressure” is
zero.

1 Bottom left plate 4 M4 screw
2 Autoloader 5 Right door
3 Autoloader cover assembly

Autoloader assembly structure

Figure 1-9 Autoloader

1 --- Reflection sensor 2 --- Unload unit

3 --- Loading counter 4 --- Tube detection assembly
5 --- Microswitch 6 --- Vertical feed unit(X load)
7 --- Horizontal load unit(Y feed) 8 --- Sample compartment assembly

1-24
Mechanical System

Figure 1-10 Autoloader (hide the cover to reveal sensor position)

1 --- X load initial sensor 2 --- X load end sensor

3 --- Tube detection position counting sensor 4 --- Tube pierce position counting sensor
5 --- Y feed initial sensor 6 --- Unload sensor

Note: The sample compartment assembly sensor is not marked in the figure above.

Debug
 Autoloader forward/backward and left/right adjustment (mechanical approach)
 Sample probe forward/backward adjustment (software approach)
 Sample probe up/down adjustment (software approach)

The first 2 steps affect centering of the sample probe, and the third step affects minimum aspiration
volume.
The detailed procedure is as follows:
1) Put a 12X75 evacuated collection tube to a tube rack, load the rack and make sure the tube is
at the autoload pierce position, meanwhile the 2 rack rollers of the autoloader are locked to the
tube rack.
2) Tap “Check” button of the “AL Piercing Pos.” in the “Debug” screen of the IPU, the sample probe
will pierce the evacuated collection tube.

Check if the pierce position is in the center of the tube (you may cover the tube with adhesive tape
for easy observation), if there is clear deflection, tap “Return” to withdraw the sample probe and
continue the adjustment as instructed by the following procedure:
① If the pierce position is not in the center from left to right, loosen the 3 screws that fix the
autoloader, make sure the autoloader is appressed to the front plate, and then move the
autoloader laterally before tightening the screws.

1-25
Mechanical System

Left screw Center screw Right screw

② If the pierce position is not in the center from front to back, make sure the autoloader is
appressed to the front plate (mechanical approach), and adjustment the forward/backward
position of the sample probe by tapping “Forward” and “Backward” buttons in the “Debug”
screen (software approach).
③ After the adjustment, tap “Check” again to make sure the sample probe pierce position is in the
center.
3) Remove the cap of the evacuated collection tube and then put it back to the tube rack. Tap
“Check” button of the “AL Piercing Pos.” in the “Debug” screen of the IPU, the sample probe will
pierce the evacuated collection tube. Lift the tube so that the tube bottom touches the probe tip,
check if the lifting height is 3～5mm (you may check the height by drawing a line on the tube).

1.2.16 Sample Compartment Assembly

 Tools
NO.107 cross-head screwdriver
 Procedure
1） Take out the autoloader.
2） Remove the 3 M4*8 screws in the bottom plate of the sample compartment assembly wi
th the NO.107 cross-head screwdriver.
3） Lift the sample compartment assembly (3106) up to take it out.

1-26
Mechanical System

No. Name No. Name

1 Sample compartment assembly 3 Bottom plate of the assembly
2 M4*8 screw
Inner structure of the sample compartment

Figure 1-11 Sample compartment assembly

1 --- Bracket 2 --- Tension spring

3 --- Sensor 4 --- Sensor baffle
5 --- Torsion spring 6 ---Damper gear

1-27
Mechanical System

 Debug
See the autoloader adjustment procedure for details.

1.2.17 Unload Unit

 Tools
NO.107 cross-head screwdriver
 Procedure
1） Take out the autoloader.
2） Remove the 2 M4*8 screws in the bottom plate of the sample compartment assembly wi
th the NO.107 cross-head screwdriver.
3） Take the unload unit up.

Tube detection assembly

 Tools
NO.107 cross-head screwdriver
 Procedure
1） Open the top right cover.
2） Remove the 2 M3*8 screws in the tube detecting and scanning bracket with NO.107 cro
ss-head screwdriver.
3） Lift the tube detection assembly up to take it out.

1-28
Mechanical System

No. Name No. Name

1 Tube detection assembly 3 M3*8 screw
2 Tube detecting and scanning bracket

1.2.18 Temperature Control Assembly

 Tools
M3 inner hexagon spanner
 Procedure
1) Open the top right cover.
2) Disconnect the tubes and wires connected to the temperature control assembly.
3) Remove the 4 M4 screws in the front cover with M3 inner hexagon spanner.
4) Take out the temperature control assembly.
 Prime
1) After replacement, enter the software screen, tap “Setup→Empty/Prime” to prime the tubes.
2) Tap “Eluent A Channel” to perform the priming.

1 Top left cover 3 Screen bracket
2 Touchscreen 4 M3 screw

List of FRUs for Replacement

Serial No. Name FRU Code

1 115-024960-00 Sampling assembly
2 041-001013-00 CT probe wipe
3 115-014677-00 Piercing probe
4 115-025076-00 Mix bath assembly
2-way miniature electromagnetic valve (stub, new
5 115-010089-00
terminal)
6 115-025078-00 Syringe assembly
7 115-011898-00 100ul lead screw driving syringe assembly
8 115-012523-00 100ul syringe (lateral)
9 115-031800-00 2.5ml syringe assembly
10 0033-30-74613 2.5ml Mindray syringe (fluororubber)
11 115-013056-00 Fluidic valve. 3-way -75~250kPa DC12V
12 115-031984-00 Auto mix assembly
13 115-006303-00 Spring tube pincher(5380)
14 115-025077-00 Plunger pump assembly
15 082-002128-00 Plunger pump 5Mpa 4.5mL/min 0.169uL/step
16 115-031854-00 Burkert valve
17 115-024961-00 Waste pump assembly
18 3001-10-07252 Rotation diaphragm pump
19 047-012739-00 Pump assembly label (P1)
20 3101-20-68592 Pump connecting line
21 115-027228-00 Degasser assembly

1-32
Mechanical System

22 082-001943-00 Degasser, 2 channels, 285ul/channel

23 009-004752-00 Degasser connecting line
24 082-002127-00 Pulse damper 2Mpa, volume 0.2mL
Rotation feeding valve, 2-position, 6-way, 41MPa
25 082-001931-00
24VDC
26 115-027283-00 HWS cistern
27 115-027284-00 Eluent A cistern
28 115-027285-00 Eluent B cistern
29 115-018614-00 Short cistern
Isolation chamber (mold MR34949-01/MR34949-0
30 3003-20-34949
2)
31 082-001963-00 Air filter 0.1um 98mmX32mm 0.1Mpa
32 115-031985-00 Autoloader
33 115-012506-00 Sample compartment assembly (3106)
34 3102-30-69247 Unload unit
35 115-005375-00 Tube detection assembly
36 115-024958-00 Power supply assembly
37 115-027265-00 Temperature control assembly
38 115-031986-00 Filter assembly
39 115-025527-00 Top left cover assembly (H50)
40 021-000058-00 Touchscreen, resistive, 8.4", 4-wire
41 115-025528-00 Screen assembly

1-33
Chapter 2 Fluidic System

2.1 Introduction
The fluidic system of H50 Automated Glycohemoglobin Analyzer mainly consists of 4 parts, which
are reagent management, sample preparation, chromatography and temperature control. Their
functions are:
Reagent management: priming, replacing and detection of the hemolysis solution, eluent A and
eluent B.
Sample preparation: aspirating sample and sending sample to the sample loop.
Chromatography: sending sample to the column, eluting sample with the 2 types of eluent; the
eluted sample then goes onto the flow cell for concentration monitoring.
Temperature control: adjusting and controlling temperature of the column and flow cell.

2.1.1 Sample Preparation Channel

1 2
6 3
5 4

HWS
cistern Injection Valve1

Sample Loop 4 uL

SV18 SV19

SV20

SV01
Syringe 100uL

SV04
SV03 SV02 Probe
wipe

Sample
probe

J10-T63 Mix Probe wipe

bath filter

C53 C54

SV06 SV05 T87

Syringe 2.5mL
C55

Mix bath filter

GP1
Waste
T73 container
SV15
Waste pump

Figure 2-1 Sample preparation channel

Reagent used: Hemolysis solution

The sample preparation channel collects, mixes and prepares sample; the channel consists of the
following 3 portions:
1) Whole blood sample collection: this channel consists of the 100ul syringe and sample probe; it
2-1
Fluidic System

collects sample of the whole blood mode, aspirated 16ul of whole blood and dispensing 4ul of blood
sample.
2) Predilute sample collection: this channel consists of the 2.5ml syringe, SV04, SV19, injection
valve, sample loop and sample probe; its major function is collecting samples of the predilute mode,
aspirating 160ul of the sample.
3) Mix bath: this channel consists of the 2.5ml syringe, SV04, SV02, mix bath, mix bath filter, SV15
and the waste pump; its major function is dispensing fluid into the mix bath, mixing sample, cleaning
the mix bath, and preparing 400ul of sample at the ratio of 1:100.
2) Sample preparation: this channel consists of the 2.5ml syringe, SV04, SV19, injection valve,
sample loop and sample probe; its major function is preparing sample, aspirating 160ul of sample.
3) Sample probe cleaning: this channel consists of the 2.5ml syringe, SV04, SV03, SV19, SV01,
probe wipe, injection valve, sample loop, probe wipe filter, SV06, waste pump, sample probe and the
probe wipe; its major function is cleaning sample preparation tubing, and the exterior and interior
walls of the sample probe, aspirating reagent from the HWS cistern (driven by the 2.5ml syringe) to
clean the components together with the valves, and collecting waste generated from cleaning the
sample probe.

2.1.2 Chromatographic Channel

The chromatographic channel mainly consists of the plunger pump A, plunger pump B, pulse
pumper, injection valve, rotation valve, column, flow cell and the waste cistern. See the following
diagram for its connection.

T98
Steel
tube 3
C41
C42
1 2 1 2

Detecor
Preheating Ta3
steel tube 2 C40
6 3 6 3
5 4 5 4 C39
C60 C59
Inject（POSA） T97 Thermostatic Chamber
Load（POSB）
C67 1 2
T77 T80 6 3
INJ_PUMPB C26 C61 C64
5 4
T85 Column
C62 C63 C38 C37
Injection Valve2 T84
T96
T79
C25
T82
C27 1 2 C32 C36
Eluent B T40 T43 Damper C35 C66
C74 C75 6 3 Preheating C65
T78 C28 C31 steel tube 1
SV17 C33 C34 5 4
INJ_PUMPA T100
C24 C29 C30 T99
Pressure Transduser Injection Valve1 OPEN
Ta1 T102
J7-T57
Ta2 GV13

Sample Loop 4 uL
C23 Waste cistern
Sample Needle

T92
Syringe

Eluent A T41 T42 C52

C76 C77
SV16 Waste
T76
cistern

Figure 2-2 Chromatographic channel

The plunger pump A (INJ_Pump A) provides feeding power of eluent A. The plunger pump B
(INJ_Pump B) provides feeding power of eluent B. SV17 and SV18 controls the on/off of the tubes of
plunger pump A and B respectively. When the plunger pump works, the corresponding valve opens,
and vice versa.
The pulse damper is a passive fluid device, it is used to buffer the pressure pulsation generated by

2-2
Fluidic System

the plunger pump. It also acts as a pressure sensor to record pressure over the tubes.
Injection valve 1 connects the sample preparation channel and chromatographic channel. When it is
at the load position, the sample preparation channel loads diluted blood sample to the sample loop;
and when it is at the inject position, the chromatographic channel sends sample to the column for
elution and separation.
The column is a critical component for chromatography. When blood sample is loaded to the column,
it is processed by eluent of the pre-defined low ionic strength and pH conditions. Due to different
charge strength and adsorption capacity with the stationary phase, the various types of hemoglobin
are eluted and separated.
Injection valve 2 is at position B during analysis, it shifts to position A only in certain maintenance
sequences.
The separated sample then goes through the flow cell to the waste cistern, and discharged from the
analyzer by the waste pump.

2.1.3 Temperature Control Unit

The temperature control unit consists of the column temperature-control box and the flow cell
temperature-control box. See the following figure for its fluidic connection.

Flow cell C41

temperature- Steel tube 3 C42
control
Preheating
box Detecor Ta3
steel tube 2 C40
T97
C39
Thermostatic Chamber
C60 C59

1 2
6 3
temperature-

C61 C64
control box

5 4
T85 Column
C62 C63 C38 C37
Column

T84

T82
C35 C36
Preheating
steel tube 1

Figure 2-3 Connection of the temperature control unit

The column temperature-control box keeps constant temperature of the column and preheats blood
sample and reagent that enters the column. The heating membrane heats the thermal storage base
plate, its conductive base then heats the column to target temperature and preserves the
temperature, and its preheating steel tube heats the reagent to target temperature to ensure
chromatographic effect.
The flow cell temperature-control box keeps constant temperature of the LED, light absorption
detector, and flow cell, and preheats blood sample that enters the flow cell. The heating membrane
heats the thermal storage base plate, its conductive base then heats the LED, light absorption

2-3
Fluidic System

detector and flow cell to target temperature and preserves the temperature, and its preheating steel
tube heats the reagent to target temperature to ensure stability of the chromatographic analysis.

2.1.4 Reagent Management

VAC

SV09
GV10

SV08 GV11

SV07
GV12

GP1 Isolation
chamber
T91
EL_A EL_B
cistern C15 cistern OPEN
HWS Germproof
Reagent Reagent Reagent cistern filter
container container container
filter 3 filter 2 filter 1
Hemolysis & Wash
Eluent B Eluent A
Solution

SV17

Degasser Unit

SV16

Figure 2-4 Connection of reagent tubes

Vent channel
Plunger pump cleaning
Optical system waste
OPEN

GV13
Waste cistern

Probe wipe
waste

C53 C54

SV06 SV05 T87

C55

GP1
Waste
container
SV15
Mix bath
Waste pump
waste

Figure 2-5 Connection of waste tubes

Reagent management involves the HWS hemolysis solution, Eluent A cistern, Eluent B cistern,
waste cistern and waste channel. See the figure below for the fluidic connection.

2-4
Fluidic System

HWS cistern priming: the HWS cistern connects to atmospheric pressure through GV10 and the
germproof filter. To prime the HWS cistern with reagent, the waste pump (GP1), GV10 and SV07 are
open to generate vacuum for the priming; when the priming finishes, the waste pump, GV10 and
SV07 are closed. When GV 10 is closed, pressure of the cistern restores to normal, during which air
enters the cistern from the germproof filter.
The priming of EL_A and Eluent B cistern is similar to the priming of the HWS cistern.
Emptying the HWS cistern: the 2.5mL syringe aspirates reagent from the HWS cistern, and dispense
it to the mix bath, and then the waste pump discharges the reagent from the mix bath to the waste
container. Each time 2mL of reagent is discharged, 60 discharges are required to empty the HWS
cistern. See the figure below.

HWS
cistern

SV04
SV02
J10-T63 Mix
bath

Mix bath filter

T73
SV15 Waste
container
Syringe 2.5mL Waste pump

Figure 2-6 HWS cistern emptying channel

Emptying the Eluent A cistern: reagent in the cistern is driven through the rapid relief channel to the
waste cistern by plunger pump A, and then discharged to the waste container by the waste pump.
Emptying the Eluent B cistern: reagent in the cistern is driven through the rapid relief channel to the
waste cistern by plunger pump B, and then discharged to the waste container by the waste pump.
The waste cistern connects to atmospheric pressure through GV13. When performing certain
maintenance operations, the waste cistern forms a vacuum, for example, when the plunger pump is
exhausting air, SV13 closes to form a vacuum in the waste cistern together with SV05 and the waste
pump.
The waste pump turns on to collect waste together with SV05, SV06 and SV15.

2.2 Reagent System

The reagent system of H50 Automated Glycohemoglobin Analyzer consists of the hemolysis solution,
eluent A and eluent B. Their major functions are listed in the table below:

Reagent Function
Used for the pre-process of blood sample before the
Hemolysis solution
HbA1c analysis. The hemolysis solution mixes with whole

2-5
Fluidic System

blood sample to process blood cells, eliminating

interference of cell particles to the ion-exchange-based
high-performance liquid chromatography (HPLC)
method, the processed sample is used for HbA1c
analysis.
Used with the HbA1c analysis system to determine the
HbA1c percentage and substance content in the blood
samples. The eluent A and B works together for elution of
Eluent A for HbA1c
hemoglobin. The HbA1c in the sample, which hardly has
analysis
any positive charge, is the first being eluted with eluent of
Eluent B for HbA1c
the pre-defined low ionic strength and pH conditions; and
analysis
the positive charged HbA0 is eluted with the eluent of
high ionic strength. In this way, the analyzer builds up the
Hb chromatogram.

2.3 Fluidic Components and Functions

2.3.1 Degasser
 Symbol

Degasser Unit

Figure 2-7 Degasser symbol

 Appearance:

2-6
Fluidic System

Figure 2-8 Degasser

 Function:
Remove gas dissolved in reagent and remove part of the bubbles.
 Note:
1. The connector on the top left of the degasser is sealed by a white plug, while the connector on the
top right is connected to the atmosphere as shown in the figure below. Do not misplace the plug.
2. Keep the board at the back of the degasser away from water.

2.3.2 Plunger Pump

Symbol

Figure 2-9 Plunger pump symbol

Appearance

Figure 2-10 Plunger pump

Function
The plunger pump is a flow drive component. With capacity change of the sealed cavity resulted
from the reciprocating motion of the plunger in the cylinder, it realizes aspirating and discharging of
liquid. A check valve is installed on both the inlet and outlet of the pump, when aspirating liquid, the
check valve of the outlet is closed, and when discharging liquid, the check valve of the inlet is closed.
If the fluid transmitted by the plunger pump is saline reagent, to avoid creep of the seal ring caused
by crystallization of reagent, the seal ring must be washed.

2.3.3 Pulse Damper

Symbol

2-7
Fluidic System

Figure 2-11 Pulse damper symbol

Appearance

Figure 2-12 Pulse damper

Functions
The pulse damper is a pressure vessel that is used to eliminate fluid pressure pulsation or flow
pulsation in tube. It can stabilize fluid pressure and flow, thus eliminating vibration of the tube. Its
cavity is filled with a special elastic material. When the pressure increases, the material is contracted
and fluid is stored in the cavity; and when the pressure decreases, the material restores with the fluid
released.
Besides, a hydraulic sensor is integrated to the pulse damper to measure pressure of the tube.

2.3.4 Feeding Valve/Rotation Valve

 Symbol

2 1 2 1

3 6 3 6

4 5 4 5
POSA POSB

Figure 2-13 Rotation valve symbol

 Appearance:

2-8
Fluidic System

Figure 2-14 Rotation valve

 Function:
The feeding valve and rotation valve share the same material. They are only different in
function.
The feeding valve quantifies and feeds sample; the rotation valve mainly realizes
maintenance functions. By switching of the 2 valves, different channels are connected and
the functions are realized.
 Note:
1. The 6 ports shall be correctly connected with their corresponding tubes.
2. The board behind the valve shall be kept away from water, and the wire shall be correctly
connected.

2.3.5 Analytical Column

 Symbol

Column

Figure 2-15 Analytical column symbol

 Appearance

2-9
Fluidic System

Figure 2-16 Analytical column

 Function
The analytical column consists of the column jacket and stationary phase; it is the key component for
chromatography. When blood sample is loaded to the column, it is processed by eluent of the
pre-defined low ionic strength and pH conditions. Due to different charge strength and adsorption
capacity with the stationary phase, the various types of hemoglobin are eluted and separated.
 Note:
Make sure the inlet and outlet of the analytical column are correctly connected during installation.

2.3.6 Filter and Filter Element

 Symbol:

Filter

Figure 2-17 Filter symbol

 Appearance:

Filter Filter element

Figure 2-18 Filter and filter element

 Function:
The major function of the filter is to keep particles in the processed sample blood from entering the
analytical column, so as to slow down the pressure increase of the column to increase its service life.
The filter mainly consists of the cover and filter element.

2.3.7 Back Pressure Sensor

 Symbol:

2-10
Fluidic System

PS1

Figure 2-19 Back pressure sensor symbol

 Appearance:

Figure 2-20 Back pressure sensor

 Function
The back pressure sensor is a relative pressure sensor, with steel tube at its back connecting to the
atmosphere. Its major function is to measure fluid pressure at the outlet of the flow cell. The pressure
signal is transferred to flow signal of the tubes, which is directly used to calculate the
chromatography area of the various types of hemoglobin.
Pay attention to direction of the sensor during installation (put the side with the marking hole
downward, it is the inlet) to avoid accumulation of bubbles in the cavity of the pressure sensor.
Otherwise, the measured pressure may be incorrect.

2.3.8 Temperature Control of the Analytical Column

 Symbol:
temperature-
control box

Column
C38 C37
Column

T84

C35 C36
Preheating
steel tube 1

Figure 2-21 Symbol of temperature control box of the analytical column

 Appearance:

2-11
Fluidic System

Figure 2-22 Temperature control box of the analytical column

 Function:
1. Preheat blood sample and reagent that enter the analytical column; 2. Heat the analytical column
and keep constant temperature.
 Note:
The column shall be firmly installed inside the black heat-conducting base. Make the arc surface in
the middle of the column fits into that of the heat-conducting base to ensure the heat-conducting
effect.
After installing the analytical column, lock up the box to ensure the heating preserving effect.
Do not bend the steel tube of the box with heavy force to avoid block of the tube and the unexpected
change in flow and pressure.

2.3.9 Temperature Control of the Optical System

 Symbol:

Flow cell C41

temperature- Steel tube 3

control box
Detecor
Preheating steel
tube 2
C40

C39
Thermostatic Chamber

Figure 2-23 Symbol of flow cell temperature control box

 Appearance:

2-12
Fluidic System

Figure 2-24 Flow cell temperature control box

 Function:
1. Preheat blood sample and reagent that enter the optical flow cell; 2. Heat the LED, light
absorption detector and flow cell, and keep constant temperature.
 Note:
After installing optical system components, lock up the box to ensure the heating preserving effect of
the optical system.
Do not bend the steel tube of the optical system with heavy force to avoid block of the tube and the
unexpected change in flow and pressure.

2.3.10 Other Fluidic Components

2.3.10.1 Mix Bath Filter
 Symbol:

Mix bath filter

Figure 2-25 Mix bath filter symbol

 Appearance:

Figure 2-26 Mix bath filter

2-13
Fluidic System

 Function:
Filter the waste discharged from the mix bath to protect downstream fluidic components.
 Note:
Pay attention to direction of the coupling during installation, the side with a gap is its outlet.

2.3.10.2 Joint
 Symbol:
/
 Appearance:

Figure 2-27 Joints

 Function:
Connect tubes and components.
 Note:
Pay attention to the direction of the coupling.
2.3.10.3 Germproof Filter
 Symbol:

Germproof
filter

Figure 2-28 Germproof filter symbol

2-14
Fluidic System

 Appearance:

Figure 2-29 Germproof filter

 Function:
Prevent germs from entering the cistern.
 Note:
Pay attention to the direction of the filter, its inlet is connected to the atmosphere.
2.3.10.4 Probe Wipe Filter
 Symbol:

Probe wipe
filter
Figure 2-30 Probe wipe filter symbol

 Appearance:

Figure 2-31 Probe wipe filter

 Function:
Filter the impurities in waste in the probe wipe to protect downstream fluidic components.
 Note:
Pay attention to the direction of the filter.

2.3.10.5 Valves
 Symbol:

2-15
Fluidic System

Figure 2-32 Electromagnetic valve symbol

 Appearance:

SMC 3-way valve SMC 2-way valve

Mindray valve Burkert valve

Figure 2-33 Electromagnetic valves

 Function:
2-way valve: connect and disconnect the channel. When the valve is not electrified, the tube
between its inlet and outlet is disconnected, and vice versa.
3-way valve: switching of channels. When the valve is not electrified, its COM is connected to the
NO; when the valve is electrified, its COM is connected to the NC.
 Note:
The operating voltage of Mindray valve is DC 12V, and its maximum pressure is 200KPa. The
Mindray valve works via the electromagnet, and restores using spring; it should not be electrified for
long. When the electromagnetic valve is electrified, the ejector rod of the valve presses down; when
it is not electrified, the ejector rod restores. By touching the rod with your hand, you can feel its
movement easily so as to judge its status.

2.3.10.6 Syringes
 Symbol:

2-16
Fluidic System

Syringe 2.5mL Syringe 100uL

Figure 2-34 Syringe symbols

 Appearance:

Figure 2-35 100uL syringe and 2.5mL syringe

 Function:
The 2.5mL syringe is used to aspirate and prepare sample, and clean the system in predilute mode.
The 100uL syringe is used to aspirate sample in whole blood mode.
 Note: /
2.3.10.7 Sample Probe
 Symbol:
/
 Appearance:

2-17
Fluidic System

Figure 2-36 Sample probe

 Function:
The sample probe provides a corrosion resistant cavity for aspiration and dispensing of blood and
probe cleanser.
 Note:
The sample probe tip is designed tapered for its piercing function.

2.3.10.8 Probe Wipe

 Symbol:

Probe
wipe

Figure 2-37 Probe wipe symbol

 Appearance:

Figure 2-38 Probe wipe

 Function:
The probe wipe provides a cavity for cleaning of the sample probe with the effect of liquid flow, and
gathering of the waste produced.
 Note: /

2-18
Fluidic System

2.3.10.9 Waste Pump

 Symbol:

Waste pump

Figure 2-39 Waste pump symbol

 Appearance:

Figure 2-40 Waste pump

 Function:
Emptying the probe wipe, waste cistern and mix bath, generating vacuum of the waste cistern and
priming reagent to the cisterns.
 Note:
Pay attention to the direction of the waste pump.

2.3.10.10 Bath and Cisterns

 HWS cistern: stores hemolysis solution;

2-19
Fluidic System

Figure 2-41 HWS cistern

 Eluent A cistern: stores eluent A;

Figure 2-42 EL_A cistern

 Eluent B cistern: stores eluent B;

2-20
Fluidic System

Figure 2-43 EL_B cistern

 HbA1c mix bath: provides the site for sample mixing and reaction, and feeds processed
sample.

Figure 2-44 Mix bath

 Waste cistern: stores waste and generates vacuum.

Figure 2-45 Waste cistern

2.4 Fluidic Sequences

2.4.1 Startup Sequence

The general startup sequence applies to starting up the analyzer after normal shutdown.
Definition of normal shutdown: shut down the analyzer with normal analyzing function (without error)
according to the procedure stated in the Operator's Manual.
The analyzer is to be started up after normal shut down for a period not exceeding the specified
period. If the analyzer is to be idle for a longer period, the prepare-to-ship procedure shall be
performed, and the startup sequence after prepare-to-ship shutdown will be invoked.
Note:
After normal shutdown, the analyzer stops working, crystals may be produced in certain area due to

2-21
Fluidic System

reagent evaporation, bubbles may be produced due to evolution of gas dissolved in reagent or air
permeability of tubes, and cross contamination may occur due to exposure to reagent, which all
bring negative impact to the analyzer.
2.4.1.1 Components Involved
All sensors, plunger pumps, waste pumps, electromagnetic valves, rotation valves, filters, waste
cisterns, other cisterns, the sample probe assembly, probe wipe, temperature-control assembly, the
analytical column, and flow cell.
2.4.1.2 Working Order
The general startup sequence consists of 3 phases:
1. Initialization
In this phase, the actions done include hardware initialization, software initialization, user login,
electromechanical system initialization and fluidic system initialization. Its procedure is shown in the
following diagram.

Start

Turn on
temperature
control

User login

Manufature/RD/
Skip
Yes Service access
initialization?
level?
Yes No
No
Electromechanical
System initialization

Startup finishes Fluidic Succeed? No

initialization
Troubleshooting
Yes

Next phase ERROR or

ATTENTION

Figure 2-46 Startup initialization process

2. Priming and cleaning

In this phase, cleaning operations of different strength are done according to the system idle period.
PUMP CLEAN mainly cleans the seal ring of the plunger pump to prevent the seal ring from being
worn out by reagent crystals; BUFFER PRIME replaces reagent in tubes of plunger pump A and B,
and empties bubbles in the tubes to maintain flow rate. WARMING UP cleans the sample
preparation channel (including the sample probe assembly and probe wipe), it also cleans the
chromatography channel to balance the analytical column, and waits until the temperature of the
column and flow cell meets requirement.

2-22
Fluidic System

The previous phase

Cistern fluid
No Idle period≤72hr? No Idle period≤72hr?
discharge

Yes Yes

PUMP CLEAN-1
PUMP CLEAN-2
PUMP CLEAN-2
BUFFER PRIME-1

BUFFER PRIME-2
BUFFER PRIME-2
Temperature meets
requirement in specified No
period?
Temperature meets
requirement in specified No
period?
Temperature meets
Yes Yes
requirement in specified No
period?
Yes
WARMING UP-2
WARMING UP-1
WARMING UP-2

Temperature meets
No
requirement?

Yes Troubleshooting

ERROR or
Next phase
ATTENTION

Figure 2-47 Startup cleaning process

3. Ending startup
If the user enables the auto analysis after startup function, and there is tube rack on the autoloader
desk, the auto analysis procedure will be started after cleaning and priming. If not, the plunger pump
will be turned off, the startup sequence ends and the analyzer stands by.

The previous phase

Is there any
Analysis
Yes sample&&Auto
flow
analysis enabled?

Pump Stop sequence

End

Figure 2-48 Startup ending process

2.4.2 Whole Blood Analysis Mode

2.4.2.1 Sample Preparation Channel
1. 0~17s:

2-23
Fluidic System

1) Sample probe pre-piercing and cleaning: the 2.5ml syringe works together with SV04, SV01,
SV03, waste pump and SV06;
2) Sample aspiration: the 100ul syringe aspirates 16ul of whole blood;
3) Cleaning after sample aspiration: the 2.5ml syringe, SV04, SV03, waste pump and SV06.

1 2
6 3
5 4

HWS
Cistern Injection Valve1

Sample Loop4 uL

SV18 SV19

SV20

SV01
Syringe100uL

SV04
SV03 SV02 Probe
wipe

Sample
probe

J10-T63 Probe wipe

Mix bath filter

C53 C54

SV06 SV05 T87

Syringe2.5mL
C55

Mix bath filter

GP1
Waste
container
SV15
Waste
pump

Figure 2-49 Work flow of sample preparation channel

2. 17~24s:
1) Sample probe cleaning in mix bath: the sample probe moves to mix bath lower position, V15
opens to empty the mix bath;
2) Sample probe exterior wall cleaning: the 2.5ml syringe, SV04, SV03, waste pump and SV06
3) Adding hemolysis solution: the 2.5ml syringe, V02 opens to add 100ul hemolysis solution to the
mix bath;
4) Sample blood discarding by probe wipe: the 100ul syringe dispenses 6ul of blood to probe wipe;
3. 24~34s:
1) Mix bath sample blood dispensing: the 100ul syringe dispenses 4ul of blood to mix bath;
2) Sample dilution and mixing: the 2.5ml syringe dispenses 300ul of hemolysis solution to the mix
bath to dilute and process sample blood, meanwhile the 2.5ml syringe mixes the sample by
aspirating and dispensing it 2 times, 100ul each time.
3) Sample probe exterior and interior wall cleaning: the 2.5ml syringe, SV04, SV03, SV01, waste
pump and SV06

4. 34~52s:
1) Sample preparation: the 2.5ml syringe works together with SV04 and SV19 to aspirate 160ul of
sample to the inlet of the feeding valve, which by then switches to send the sample loop to sample
preparation channel. The 2.5ml syringe continues to aspirate 80~100ul of sample through the
feeding valve. The sample loop quantifies 4ul of sample. By the 52s, the feeding valve switches to

2-24
Fluidic System

finish sample feeding.

1 2
6 3
5 4

Injection Valve
1

Sample Loop4 uL

SV19

Figure 2-50 Sample preparation process

5. 52~72s:
1) Tube cleaning and restoration: After sample feeding (the feeding valve switches after sample
feeding), the 2.5ml syringe works with SV04, SV01, SV19, waste pump, SV06, SV02 and SV15 to
clean the sample preparation tube and mix bath.
2.4.2.2 Chromatography Channel
1． 0~99s
Plunger pump A works with stable flow, when the feeding valve moves to the load position, it sends
sample to the analytical column for elution.
2． 99~104s
Plunger pump A stops working, and plunger pump B starts to work. It feeds eluent B with stable flow
to further elute sample in the analytical column.
3． 104~124s
Plunger pump B stops working, and plunger pump A starts to work. It feeds eluent A with stable flow
to wash the analytical column and restore its balance.

2.4.3 Predilute Analysis Mode

2.4.3.1 Sample Preparation Channel
4． 30~52s
1) Sample preparation: the 2.5ml syringe works together with SV04 and SV19 to aspirate 160ul of
sample to the inlet of the feeding valve, which by then switches to send the sample loop to sample
preparation channel. The 2.5ml syringe continues to aspirate 80~100ul of sample through the
feeding valve. The sample loop quantifies 4ul of sample. By the 22s, the feeding valve switches to
finish sample feeding.
5． 52~72s
1) Tube cleaning and restoration: After sample feeding (the feeding valve switches after sample
feeding), the 2.5ml syringe works with SV04, SV01, SV19, waste pump, SV06, SV02 and SV15 to
clean the sample preparation tube and mix bath.
2.4.3.2 Chromatography Channel
1 0~99s
Plunger pump A works with stable flow, when the feeding valve moves to the load position, it sends

2-25
Fluidic System

sample to the analytical column for elution.

2 99~104s
Plunger pump A stops working, and plunger pump B starts to work. It feeds eluent B with stable flow
to further elute sample in the analytical column.
3 104~124s
Plunger pump B stops working, and plunger pump A starts to work. It feeds eluent A with stable flow
to wash the analytical column and restore its balance.

2.4.4 Probe Cleanser Maintenance Sequence

After long-term use, particles in blood sample may block the filter before the analytical column, which
causes pressure rise in tubing. The pressure may even rise to the maximum operating pressure.
Therefore probe cleanser maintenance shall be performed to the filter based on the quantity of
samples analyzed.
The maintenance flow is as follows:
Start

Software prompt asks the user to

put probe cleanser into the sample compartment

Open the
compartment door
Tap “Start”

Is the compartment
door closed?

Yes
Preparation before
maintenance
Probe Cleanser
maintenance
Reset after
maintenance
No

Reset times

Maintenance
performance self-test
Prompt for
calibration

End

Figure 2-51 Probe cleanser maintenance flow

Preparation before maintenance

① Turn on the degasser; ② Prime all cisterns to make sure there will be enough reagent to
support the probe cleanser maintenance; ③ Switch the rotation valve to fast relief position to
prevent probe cleanser from entering the analytical column.
Note:
The analytical column may be damaged if probe cleanser enters the column. Should there be
any error during the preparation phase; the probe cleanser maintenance process will end instantly.

2-26
Fluidic System

Probe cleanser maintenance

The maintenance flow consists of 2 phases:
① Probe cleanser soaking
Plunger pump A turns on to keep the pressure and flow of the tubing of eluent A stable;
The sample probe moves to the closed sample aspiration position, the 2.5uL syringe aspirates 60uL
of probe cleanser and cleans the outside of the sample probe;
The syringe keeps on aspirating probe cleanser, which goes through the sample loop first. With
continuous switching of the feeding valve and driven by the plunger pump, the probe cleanser in the
sample loop goes to the filter and is discharged to the waste cistern then through the fast relief
channel.
When the probe cleanser goes to the filter, plunger pump A stops to reduce its flow rate so that it can
thoroughly soak the filter.
The steps above are performed twice in a row.

T98 Waste cistern

T97 Flow cell

C39

Plunger pump T80 C60 C59

1 2
6 3 T85 Column
C61 C64 C38 C37
5 4
T96
C62 C63 T82 T84
T110 ROT-V
Filter

C35 C36
C27 1 2 C32
Preheating
6 3 steel tube 1
C28 C31
5 4 Ta1
C29 C30
INJ-V C47

Ta2
J7-T57-J13-T111 Sample Loop 4 uL T60(P1)

C86
J9
T56 SV19

2.5mL syringe T93

C58

Probe
wipe

Sample
probe

Figure 2-52 Probe cleanser soaking

② Probe cleanser cleaning

The purpose of probe cleanser cleaning is to remove residual probe cleanser in tubing to eliminate
its impact to blood sample and the chromatography system. The sample preparation channel and
chromatography channel are cleaned.
The 2.5mL syringe aspirates hemolysis solution from the HWS cistern, pulls and pushes the
hemolysis solution back and forth in the sample preparation channel to clean it, and then discharges
the solution to waste cistern through the sample probe and probe wipe. During the process, the

2-27
Fluidic System

feeding valve switches between POS A and POS B repeatedly for thorough cleaning.
Cleaning of the chromatography channel is accomplished by the continuous and intermittent liquid
feeding of Plunger pump A, which soaks and cleans the downstream tubing so that it can restore its
normal state.
Restoration after maintenance
After probe cleanser cleaning, the state of the fluidic system shall be restored to normal. The
operations to be done include switching rotation valve, priming cisterns and turning off the degasser.
Maintenance performance self-test
After probe cleanser maintenance, self-test of the filter pressure and column pressure shall be
performed to see if the maintenance effect and filter performance are ok.

2-28
Chapter 3 Optical System

3.1 Overview of Optical System Principle

The H50 optical system uses double wavelength colorimetric principle to measure the hemoglobin
solution eluted from the chromatography column real time, by measuring the absorbance curve
(chromatogram), concentration of the hemoglobin solution can be obtained. The measuring
wavelength is 415nm, while the reference wavelength is 500nm, which is used to offset temperature
drift and background interference to increase measuring accuracy.

3.2 Optical System Operating Principle

The following figure is the optical path top view of the H50 optical system.

9
OUT

1 2 3 4 6 7 8
IN

Figure 3-1 Optical path top view

1. Blue LED
2. Convergent lens
3. Encapsulated lens
4. Flow cell
5. Encapsulated lens
6. Dichroscope
7. 500nm interference filter
8. Detecting PD
9. 415nm interference filter
10. Detecting PD
The H50 optical system uses the blue LED (1) as optical source, which emits blue light beam whose
dominant wavelength is 460nm. The light beam is then converged by the convergent lens (2), and
then enters the flow cell (4) which is encapsulated by the two planoconvex quartz lenses (3, 5). The
emergent light is split into two beams by the dichroscope (6), the one whose wavelength is 500nm is
transmitted through the interference filter (7) and the received by the detecting PD (8), while the
other one whose wavelength is 415nm is reflected through the interference filter (9) and the received
by the detecting PD (10). Since hemoglobin has intensive absorption to the 415nm light beam, its
concentration can be calculated by measuring the absorbance. To increase the measuring accuracy
and reduce interference, the 500nm light beam to which hemoglobin has no absorption is used as a

3-1
Optical System

reference.

3.3 Optical System Composition

The following figure shows the assemblies of the H50 optical system.

8
1 2 3 4 5
7

Figure 3-2 Optical system composition

1. Detecting assembly 2. Flow cell assembly

3. Front optical assembly 4. Cover
5. Outlet pipe 6. Inlet pipe
7. Base plate assembly 8. Shielding case
The detecting assembly (1) is consisted of the dichroscope, 415nm and 500nm interference filters,
and detecting PDs. The flow cell assembly (2) is consisted the flow cell and encapsulated quartz
lenses. The flow cell assembly (2) is fixed to the detecting assembly (1). The front optical assembly
(3) includes the blue LED and aspheric focusing lens.
Part of the inlet pipe (6) is embedded in the base plate assembly (7) which is fitted with heating
membrane to adjust temperature of the sample that goes to the flow cell. The entire optical system is
encapsulated by the shielding case (8) to shield the impact of outside light, heat, vibration and
electromagnetic field.

3.4 Optical System Status Determination

The status of the H50 optical system can be determined by voltage and gain of the two optical

3-2
Optical System

channels in the Setup -> Gains screen.

First, run a background count under CT-WB mode. After the analysis,, check if the voltages of the
415nm and 500nm optical channels are within the range 3.800V～4.100V, and the gains are below
255 in the Setup -> Gains screen. See the following figure.

Figure 3-3 "Gains" screen

3.5 Maintenance and Replacement of the Optical System

If the optical system is not in its normal state, maintenance or replacement of the system is needed.
Based on the status determination, the possible causes and maintenance methods of the optical
system anomaly are:
 Cause 1: Optical intensity attenuation due to aging of the LED
Optical intensity of the LED attenuates due to its aging, which results in voltage drop of the PDs of
the 415nm and 500nm channels.
 Tool: No. 107 cross-head screwdriver
 Solution:
a) Adjust the gains of the 415nm and 500nm channels in the Setup -> Gains screen until
the voltages fall within the range 3.800-4.100V.
b) If the gains have been adjusted to the max, but the background voltages are still lower
than the required range, the H50 optical system must be replaced according to the
following procedure.
i. Shut down the analyzer, and then disconnect the sockets that connect the 3
wires of the optical system to the analyzer system (see the following figure). The
signal wire is connected to socket A42 and A45 of the drive board of the analyzer.

3-3
Optical System

Wire connecting the

Optical system temperature switch and
signal wire heating membrane

Temperature
sensor wire

Figure 3-4 Wires of the optical system

ii. Remove the 4 M3 cross-recessed panhead screws (2 in the back and 2 in the
front) that fix the optical system to the support plate of the analyzer, and unscrew
the joints of the inlet and outlet pipes. See the following figure.

Inlet pipe joint

Outlet pipe
joint

M3 screws on the
front

Figure 3-5 Connection of the optical system to the analyzer

iii. Replace the optical system and connect it back as shown in the 2 figures above.
Be noted that the temperature sensor wire of the base plate shall be connect to
terminal T_OPT_HT, and the temperature sensor wire of the flow cell shall be
connected to terminal T_OPT_AIR; and the inlet and outlet pipes shall be
tightened to avoid leakage.
 Note:
 Before the adjustment, run a background count in the "Sample Analysis" screen to
make sure the flow cell is filled with liquid;
 After replacing the optical system, make sure the inlet and outlet pipes are
tightened to avoid leakage.
 Cause 2: Failure of the LED drive board or the detecting assembly
 Tool: No. 107 cross-head screwdriver

3-4
Optical System

 Solution:
a) If no change in voltage is observed when adjusting the gains of the optical channels,
the LED drive board or detecting assembly may be broken. As the LED drive board is
fixed inside the optical system shielding case, and the detecting assembly is fixed to
the base plate and connected to the inlet and outlet pipes as well, they can be difficult
to be replaced, the workable solution is to replace the entire H50 optical system. The
replacing procedure has already been given in Cause 1, step b).

3.6 Optical System Status Verification after Maintenance

or Replacement
The following items shall be verified after maintaining or replacing the optical system.
 Adjusting gains of the channels
 See Cause 1, step a) of section 5.
 Verification with samples
 After adjusting the gains, run analysis of a normal fresh blood sample. It is required that
chromatogram peak pattern of the sample is normal, without abnormal peak, as shown in
the following figure.

Figure 3-6 Chromatogram of a normal sample

3-5
Chapter 4 Hardware System

4.1 Structure of Hardware System

The hardware system mainly consists of 3 boards (namely main control board, drive board and
autoloader board) and 4 modules (namely autoloader module, driving sub-module, optical signal
detection module and main control module). The connection diagram is shown as follows:
Optical
Optical pre-
pre- Optical
Optical pre-
pre- Optical heating Optical system
Front amplification
amplification amplification
amplification membrane and temperature
cover board
board board
board protection switch sensor

LED
LED drive
drive board
board

009-004812-00 Optical
system Heating cable
009-005459-00
Cover sensor cable 009-004814-00 Optical system
temperature sensor cable

051-000218-00
051-000218-00 Liquid
Liquid pressure
pressure
detection
detection board(I)
board(I) 009-004813-00 Optical system
J4 signal cable
Column thermostatic
009-004754-00 Column
chamber heating
A38 A17 A5 A18 thermostatic chamber heating
A41，A45 membrane and
J69 J80 control cable
Touch screen protection switch
009-004817-00 009-004753-00 Column
control board J67 Column thermostatic
Touch screen touch thermostatic chamber
051-000881-00 A4 chamber
control cable
（F) temperature cable
Ambient
temperature
009-004818-00 009-004742-00
J4， A20 009-004748-00 Plunger
LED screen signal Communication M
J16 pump motor cable
cable J81 cable between A27
main control Plunger pump
A37 009-004749-00 Plunger pump
board and drive Plunger
Keyboard 051- sensor cable
009-004816-00 board pump
J78
001568-00（H) indicator board cable 009-004750-00 Rotary feedingsensor
051-001159-00
051-001159-00 Main
Main control
control board
board (B)
(B) A16
valve power cord 21
Indicator board 009-004751-00 Rotary feeding 34 56
A26
051-000675-00 (G) valve signal cable
009-004743-00
RFID reagent detection A12 Cable to the right
009-005582-00 J14
module door valve
Reagent RFID cable
(009-005588-00 RFID
009-004744-00
lead wire) *3
A13 Front horizontal
RFID reagent detection 051-001159-00
051-001159-00 Drive
Drive analog
analog board
board PCBA(B)
PCBA(B) plate valve cable
009-005583-00
module J9
Tube rack RFID cable
(009-005588-00 RFID A7 009-004745-00
wire) J68 Pump cable
J8
009-004746-00
A11 Cable to the middle
Connecting line of plate valve
data board and
autoloader board 009-004755-00 Fluid
serial port A6 pressure sensor cable

J12 J11 Fluid pressure sensor

A15
009-004752-00 Degasser
009-004756-00 Autoloading Degasser cable pump
Autoloader
mechanism and sensor extension J3,J14
board
cables ，J7,J8 009-004747-00 Floater sensor
A30
cable Floater
051-000393-00
051-000393-00 Autoloader
Autoloader board
board (B)
(B) A1
009-004811-00
Mix mechanism 009-004763-00 Mix mechanism motor A32 009-
J15,J16 Syringe sensor cable
and sensor connecting line 002604-00
,J6
Syringe
J5
M ground
（6P） A14 A33 A21 009-004810-00 Syringe
A22 motor cable cable
100uL syringe
2.5mL syringe
009-004739-00 Main
009-004740-00
control board cables 009-004741-00 Drive
Autoloader board 009-004809-00
to digital and drive board cables to digital
power cord Sample
board analog power and power circuit
circuit collection
assembly
sensor
connecting
J5 J7 J6 cable 009-002463-00
J3 M_SAMPLE_Y
（6P）（6P）（12P）（14P） Sample collection SEN_XPOS
220V/110V AC 009-004808-00 Joint assembly motor and SEN_YUP
Sample sensor patching line
Power
Power supply
supply module
module J2 collection
（12P）
assembly M
motor
connecting SAMPLE_X
051-001345-00
051-001345-00 Power
Power patching
patching board
board PCBA(C)
PCBA(C) cable

Figure 4-1 Hardware system connection diagram

1. Main Control Board

The main control board provides the following functions:
1. FPGA configuration interface: use the SPI bus line for chip configuration and update;
2. USB port: the communication port supports 4 USB 2.0 devices;
3. UART interface: supports 7 channels of serial communication at most;
4. Network interface: 10M/100M Ethernet communication interface;
2
5. Touch screen control board uses I C communication protocol to communicate with main
control board;

4-1
Hardware System

6. RFID interface: the RFID interface uses the RS232 voltage level standard, and send signals
to control the electrifying and de-electrifying of the RFID.
7. Indicator board: the indicator board acts as the prompter to the analyzer and communicates
with the main control board through common IO voltage level;
8. Autoloader communication: the serial port of autoloader board uses the RS422 voltage level;
9. Display interface: supports 18 bit display mode; and its backlight socket is controlled by the
switching between high and low voltage levels.

2. Drive Board
The drive board controls the movement of major mechanisms, performs pressure detection and
drives various components and parts. It mainly provides the following functions:
1. Driving stepping motors: drives 6 stepping motors
2. Sensor signal detection: detects the signals of 8 sensors;
3. Driving electromagnetic valves: drives 21 electromagnetic valves;
4. Air pressure detection: supports vacuum detection;
5. Fluid detection sensor: supports the floater sensor;
6. Analytical column pressure detection: detects the column pressure;
7. Drive of degasser: control the voltage switch of the degasser;
8. Temperature detection and control: use temperature sensors to detect temperature and
controls the heating of optical system and column.

3. Autoloader Board
The autoloader board controls the movement of the autoloader the mix mechanism. It mainly
provides the following functions:
1. Driving the stepping motors;
2. Sensor signal detection;
3. Mix mechanism tube detection;
4. Scanner control;
5. Control of the electromagnet valve to the sample compartment door.

4. Optical Signal Detection Module

The optical signal detection module consists of two pre-amplification boards and an LED drive board.
While the LED drive board provides constant current to drive the LED, the pre-amplification boards
support the optical signal detection of 415nm and 500 nm channels.
1. LED drive board: supports the constant drive of LED; the adjustment of the current is
supported by an adjustable potentiometer;
2. Optical pre-amplification board: the optical signal detection of both 415nm and 500nm
channels.

4-2
Hardware System

4.2 Hardware System Signal Flow Chart

The main control board is the control center of the whole hardware system. Almost all the signals will
finally converge on the main control board.
1. Analog drive board components—>Analog drive board--Main control board
2. Autoloader board components-Autoloader board-Main control board
3. Main control board components- Main control board

Hardware system Components

Indicator board LED drive board

Optical system
Optical pre- LED，415nm optical channel
Display screen
amplification board 1 PD: 500nm optical channel PD
assembly

Optical pre-
amplification board 2
Touch screen
control board Temperature detection: detection
of inside-analyzer temperature,
optical system temperature and
column temperature
Analog drive
USB interface board Heating control: temperature
control of the optical system and
the column
Main control board
Network interface
Sample collection assembly
motor control, sensor detection
Column pressure detection
Back pressure sensor
detection board AM335X Valves, pumps and floater
module Rotary feeding Rotary feeding valve
valve control board
RFID card reader1
Rotary feeding Feeding valve
valve control board
RFID card reader2

RFID card reader3

Autoloader (stepping motors,
Autoloader detection sensors, micro-switch,
RFID card reader4
board electromagnet)
Mix mechanism (stepping
motors, sensors)

Figure 4-2 H50/H50P hardware subsystem signal flow diagram

4.3 List of Hardware Major PCBA and Cables

4.3.1 Components of main boards

051-001062-00 Indicator board PCBA
LCD screen TFT 8.4＂800＊600 3.3v 450cd/m2 LED
021-000060-00
backlight
021-000058-00 Touch screen, electric resistance, 8.4＂, 4-lead
051-000881-00 6301 touch screen control board
051-001568-00 Printing module keypad PCBA
051-000393-00 Autoloader board PCBA
051-001345-00 Power patching board PCBA
051-001765-00 System driver board PCBA
051-001944-00 3008 main control board PCBA

4-3
Hardware System

051-001983-00 System LED drive board PCBA

051-001982-00 Pre-amplification board PCBA
115-031818-00 RFID card reader
051-001277-00 Power board PCBA
051-000218-00 Clog detection board

4.3.2 Cables
No. Part No. Material description
1 009-004739-00 Main control board cables to digital and
drive board analog power circuit
2 009-004740-00 Autoloader board power cord
3 009-004741-00 Drive board cables to digital and power
circuit
4 009-004815-00 Communication cable of data board and
autoloader board serial port
5 009-004742-00 Communication cable of main control board
and drive board
6 009-004743-00 Cable to the right door valve
7 009-004744-00 Cable to the front plate valve
8 009-004745-00 Pump connecting cable
9 009-004746-00 Cable to the middle plate valve
10 009-004747-00 Floater sensor cable
11 009-004748-00 Plunger pump motor cable
12 009-004749-00 Plunger pump motor sensor cable
13 009-004750-00 Power cable to the rotary feeding valve
14 009-004751-00 Valve cable to the rotary feeding valve
15 009-004753-00 Column thermostatic chamber temperature
control cable
16 009-004754-00 Column thermostatic chamber heating
control cable
17 009-004755-00 Fluid pressure sensor cable
18 009-004756-00 Autoloading mechanism and sensor
extension cables
19 009-004808-00 Sample collection assembly motor
connecting cable
20 009-004809-00 Sample collection assembly sensor
connecting cable
21 009-004810-00 Syringe motor cable
22 009-004811-00 Syringe sensor cable
23 009-004812-00 Optical system heating module cable
24 009-004814-00 Optical system temperature sensor cable
25 009-005459-00 Cover sensor cable

4-4
Hardware System

27 009-005582-00 Reagent RFID cable

28 009-005583-00 Tube rack RFID cable
29 009-002604-00 Syringe earth wire
30 009-005259-00 LED cables
31 009-004813-00 Optical system signal line
32 009-004759-00 Autoloading mechanism motor and sensor
cables
33 009-002463-00 Sample collection assembly motor and
sensor patching line (CT models)
34 009-004763-00 Mix mechanism motor and sensor
connecting line
35 009-004752-00 Degasser connecting line
36 0020-20-12522 Power cord (GB/10A250V/2.5M)
37 009-004056-00 LIS system network connecting cable
38 009-004817-00 Touch screen control cable
39 009-004818-00 LCD screen signal cable
40 009-001198-00 Front cover protective earth wire
41 009-004816-00 Key board cable

4.4 Distribution Diagram of Power Supplies

The power supply module outputs 3 types of power circuit: digital power circuit, analog power circuit
and power circuit. The circuits are input through the J1 socket on the power patching board and then
distributed to various boards through the rest sockets on the power patching board. The power
distribution diagram is shown as following:

051-001159-00 Main control board

A1(A12V,
AN12V)
D5V D12V(J68)
J2
（12P） 051-001159-00 Analog drive board
PCBA(B)
J6 A14（D5V D12V
J5 J7 （12P） P24V)
（6P）（6P） J3
051-001345-00 Power patching board PCBA(C)
（14P）
J5
（D5V
051-000393-00 Autoloader board (B)
P12V
P24V)

Figure 4-3 System power distribution diagram

4-5
Hardware System

4.5 Introduction to the Hardware Boards

4.5.1 Main Control Board

Function overview
As the control center of the analyzer, the main control sub-module regulates the running of the whole
system. It is the "central nervous system" of the analyzer. On the other hand, it also controls the
man-machine interaction functions (for example, the screen display, response to touch actions, and
the network interfaces). In this respect, it also can be seen as the "brain" of the analyzer.

Autoloader board
Display screen communication
board

Touch screen
Indicator board
control board
AM335X

Liquid pressure Main control

USB*4
detection board sub-module

Drive board FPGA

communication Network interface
socket

RFID*4

Figure 4-4 Main control sub-module structure

The main functions of the main control platform are as follows:

 support the TFT screen with display resolution of 800*600;
 provide touch screen interfaces;
 provide serial/SPI communication interface to the drive control platform;
 provide 4 USB 2.0 ports;
 provide 10M/100M network interface;
 support autoloader board communication;
 provide RFID communication interface.

4-6
Hardware System

Socket Description

Figure 4-5 Main control board structure

Table 4-1 Sockets on the main control board

Position Description
No. Function
no.
1 J78 Indicator board socket /
2 J81 Drive board communication socket /

3 J8 Autoloader board communication /

socket
4 J80 Liquid pressure detection board /
communication socket
5 J69 Liquid pressure detection board /
power supply socket
6 J68 5V and 12V power sockets /

7 J9 Tube rack RFID communication /

socket
8 J16 Screen LVDS socket /

4-7
Hardware System

9 J61 SD card socket Located at the back of the board.

Pay attention to the card insert
direction
10 J4 Display screen backlight socket /

11 J67 Touch screen socket /

12 J14 Reagent RFID communication /

socket
13 B1 Button cell port Insert the cell in accordance with the
"Top and Bottom" side marks at the
metal position of the socket.
14 J1 Network socket /

15 J2 USB ports /
16 J3 USB ports /

Indicator Description
Table 4-2 Main board indicator

Position Description
No. Function
no.
1 D2 FPGA status indicator Blue LED, flicker at the frequency
of 1Hz
2 D6 Ethernet connection status The indicator flickers when
indicator connected to the Ethernet
3 D7 10/100M Ethernet connection The indicator flickers when
status indicator connected to the 100M Ethernet
4 D40 USB status indicator
5 D42 USB status indicator
6 D44 Power conversion chip status Stay on under normal conditions
indicator
7 D1 Digital 3.3V voltage indicator
8 D5 (CPU Power conversion chip status Stay on under normal conditions
sub-module) indicator

Test points
Table 4-3 Main board test points

Position Normal voltage range (V) Description

No. Function
no.
1 TP36 5V test point 4.75 ~ 5.25 Digital circuit ground TP39

4-8
Hardware System

2 TP37 12V test point 11.4~ 12.6 (GND)

3 TP2 3.3V test point 3.13 ~ 3.47

4 TP1 1.8V test point 1.71~ 1.89
5 TP3 1.2V test point 1.14~ 1.26
Note: connect one end of the multimeter to the voltage test point, the other end to the corresponding
ground test point.
Troubleshooting
Table 4-4 Troubleshooting of main control board

No. Error description Troubleshooting

1 Display screen backlight does not light 1. Check whether the two ends of cable J4
are properly inserted
2. Measure the TP37 and TP2 voltages, and
see whether they are normal
3. If voltages measured at TP37 and TP2
are normal, and cable J4 is properly
connected and not damaged, then the
cause for the error may be that the boot
application is not burned into the CPU
sub-module, or the CPU sub-module is
damaged and need to be replaced.
2 The display screen backlight works properly, Check whether the two ends of cable J16 are
but the logon screen will not display properly inserted.
3 The screen display stops on the logon Reconnect the SD card.
screen, the progress bar won't display
4 Software upgrade screen is prompted when 1. Check whether the two connectors of J81
the instrument is turned on are properly connected;
2. Check whether the two connectors of J8
are properly connected;
3. Check whether the D2 indicator (blue) on
the main board is flickering at the
frequency of 1Hz;
When all above steps are performed but the
error remains, then the cables or the main
board is damaged.
5 No report when error occurs 1. Check whether the status indicator board
is properly inserted (J78); try
reconnecting it;
2. Check whether the cables are damaged.
6 Touch screen does not respond 1. Check whether the touch screen control
cables are properly connected (A12); try

4-9
Hardware System

reconnecting the cables;

2. Check whether the cables are damaged.
3. Check whether the touch screen control
board has errors.
7 The touch screen does not response at the Log in at the service access level, re-calibrate
touched position the touch screen

4.5.2 Analog Drive Board

Function Overview
The analog drive board controls the motive power components (e.g. motors and valves) and
monitors the states of the sensors, fluid level and micro-switches. It also monitors the temperature,
humidity and voltages as required. Besides, as an important part of the hardware system, the analog
drive board also supports some communication functions.

Sensor *8 21 valves

Stepping Air pressure

motor*6 sensor
MCU

Liquid
Rotary pressure
feeding valve sensor

Main control FPGA EPCS16

board SPI Floater*5
communicatio
n socket UART

Temperature
Degasser
sensor

Figure 4-6 Drive sub-module structure

Above figure shows the analog drive board structure. The board can be divided into the following
functional units by different types of controlled components:
 Main control unit: converts the control signals from the host system to corresponding action
commands; and executes the commands by controlling the moving parts movements and
monitoring sensor states.
 Control unit: controls the movements of motors, valves and fans.
 Monitoring unit: monitors the status of position sensors, analog sensors, micro-switches,
voltages and temperatures.
 Power supply: provides constant and table power to the 24V, 5V and 3.3V power circuits, as
well as the other digital power circuit and reference power circuit.

4-10
Hardware System

Socket Description
Table 4-5 Sockets on the main control board

Position Description
No. Function
no.
1 A7 Waste pump socket /
2 A41 Optical pre-amplification board signal socket /

3 A45 Optical pre-amplification board and LED drive board /

socket
4 A5 Optical flow cell temperature sensor socket /

5 A4 Column temperature sensor socket /

6 A1 Analog +12V/-12V power socket /

7 A6 Liquid pressure detection sensor socket /

8 A37 Plunger pump sensor socket /

9 A33 Sampling mechanism sensor socket

10 A32 Syringe sensor socket /

11 A31 Floater sensor socket /

12 A30 Floater sensor socket /

13 A27 Main control board communication socket /

14 A26 Rotary feeding valve drive socket /

15 A22 Sampling mechanism motor drive socket /

16 A21 Syringe motor drive socket /

17 A20 Plunger pump motor drive socket /

18 A18 Column thermostatic chamber heating socket /

19 A17 Optical system heating socket /

20 A16 Rotary feeding valve power socket /

21 A15 Degasser socket /

22 A14 Digital 5V, 12V/24V power sockets /

23 A13 Front horizontal plate valve drive socket /

24 A12 Right plate valve drive socket /

25 A11 Middle plate valve drive socket /

4-11
Hardware System

Indicator Description
Table 4-6 Analog drive board indicator

Position Description
No. Function
no.
1 D94 FPGA operation indicator Flickers at the frequency of 1Hz after
analyzer initialization
2 D65 MCU operation indicator 1 Flickers at the frequency of 1Hz after
analyzer initialization
3 D66 MCU operation indicator 3 Stay on under normal conditions
4 D16 12V power supply indicator Stay on under normal conditions
5 D17 24V power supply indicator Stay on under normal conditions
6 D96 Digital 5V power indicator Stay on under normal conditions
7 D99 Digital 3.3V power indicator Stay on under normal conditions
8 D19 Analog -12V power supply indicator Stay on under normal conditions
9 D18 Analog +12V power supply indicator Stay on under normal conditions
10 D22 Analog +5V power supply indicator Stay on under normal conditions
11 D23 Analog -12V power supply indicator Stay on under normal conditions
12 D47 Motor channel1 indicator Stays on when not working; flickers
when working
13 D48 Motor channel2 indicator Stays on when not working; flickers
when working
14 D49 Motor channel3 indicator Stays on when not working; flickers
when working
15 D50 Motor channel4 indicator Stays on when not working; flickers
when working
16 D51 Motor channel5 indicator Lights off when the motor is not
working, flickers when working
17 D52 Motor channel6 indicator Lights off when the motor is not
working, flickers when working
18 D36 Column thermostatic chamber Lights on when heating, lights off when
heating indicator not heating
19 D37 Optical system heating indicator Lights on when heating, lights off when
not heating

4-12
Hardware System

Test points
Table 4-7 Analog drive board test points

Position Normal voltage Description

No. Function
no. range (V)
1 TP7 12V power test point 11.4~12.6 Power circuit ground
2 TP14 24V power test point 22.8~25.2 TP8/TP13

3 TP1 Digital 5V power test 4.75~5.25

point
4 TP3 Digital 3.3V power test 3.135~3.465
point Digital circuit ground
5 TP6 Digital 2.5V power test 2.375~2.625 TP2/TP4
point
6 TP5 Digital 1.2V power test 1.17~1.23
point
7 TP18 Digital +12V power test 11.4~12.6
point
8 TP19 Analog -12V power test -11.4~-12.6
point Analog circuit ground
9 TP21 Digital +5V power test 4.75~5.25 TP20
point
10 TP25 Analog -5V power test -4.75~-5.25
point
Note: connect one end of the multimeter to the voltage test point, the other end to the corresponding
ground test point.

Troubleshooting
Table 4-8 Troubleshooting of analog drive board

No. Error description Troubleshooting

1 Lots of motors and sensors show 1. Burned board: check whether the components
abnormal status on the board are burned or damaged; if yes,
investigate for possible causes and replace the
damaged board if necessary.
2. Power problem: check the power supply
indicators one by one and confirm whether the
supply voltages fall in rated ranges. If any indicator
works abnormally, then the board may be
damaged.
3. Cable problem: the cables of the analyzer
generally run in bundles. Check whether there is

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Hardware System

damage or short circuit problem between cables. If

yes, replace the cables. In addition, pay attention if
the cables get loose from where they are inserted
into the boards. If so, re-insert the cables tightly.
2 One of the motor channels is abnormal 1. Check the powers: check the powers one by
one and see whether their indicators and voltages
work normally;
2. MCU and FPGA status: check whether the 3.3V
power works properly, and whether the MCU and
FPGA indicators normally flicker;
3. Logic run: check whether the indicators of the
motor channels work as expected;
4.Cables: check whether the cables are damaged,
broken or short circuited; check whether the
cables are tightly inserted into the boards and the
sockets of the motors;
5. Motors: when all the above checking points are
OK, replace the motor.
3 One of the sensors work abnormally 1. Check the powers: check the powers one by
one and see whether their indicators and voltages
work normally;
2. MCU and FPGA status: check whether the 3.3V
power works properly, and whether the MCU and
FPGA indicators normally flicker;
3. Logic run: check whether the indicators of the
motor channels work as expected;
4.Cables: check whether the cables are damaged,
broken or short circuited; check whether the
cables are tightly inserted into the boards and the
sockets of the motors;
5. Sensor problem: when all the above checking
points are OK, replace the sensor and check
again.
4 Abnormal communication to main When the power supply to the board is OK, and
control board the MCU and FPGA work properly, first check
whether the cables are damaged and if are tightly
inserted into the boards.
5 Temperature abnormal Check the temperature cables are properly
connected, whether they are damaged. If there is
no such problem, check whether the temperature
sensor gets short-circuited.
6 ADC reads abnormal sensor values Check the temperature cables are properly

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Hardware System

connected, whether they are damaged. If there is

no such problem, you may need to replace the
sensor.

4.5.3 Autoloader Board

Introduction
The autoloader board is mainly responsible for blood sample presentation, mixing and the reading of
the tube barcode.

Function
Driving the stepping motors

The autoloader board controls the driving of the following 5 stepping motors:
Mix mechanism X direction motor
Mix mechanism Z direction motor
Mix motor
X direction loading motor
Y direction feeding motor
The stepping motors use the bipolar constant current half step driving method, with each stepping
motor having its own position sensor.

Control function of electromagnets

The electromagnets control the open of sample compartment door (the door need to be closed
manually).

Control of built-in scanner

The built-in scanner reads the barcodes of the tubes and tube racks. The autoloader board controls
the built-in scanner through the MCU. MCU realizes asynchronous serial communication (UART)
with built-in scanner through serial port 1.

Status detection of position sensors and micro-switch

On the autoloader board seat 13 position sensors and a micro-switch. The FPGA detects the status
of position sensors and the micro-switch from time to time; and the MCU inquires the status data
stored in FPGA as needed.

Parameter storage

You may need to adjust the start positions of the mix mechanism X direction motor and the mix
motor to remove processing and installment deviations. The position parameters after adjustment
will be saved to the autoloader.

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Hardware System

Structure

Connections of the Autoloader board

Sockets
The sockets on the autoloader board
Sockets Functions Pins Description
J3 Electromagnet socket 2 /
J5 Power socket 6
J6 Mix mechanism sensor socket 20
/
J7 Feeding mechanism sensor socket1 20
J8 Feeding mechanism sensor socket2 20
J11 MCU configuration socket 10 /
J12 Communication socket 4 /

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Hardware System

J14 Feeding mechanism motor socket 8 /

Socket to mix mechanism X motor /
J15 8
and Z motor
J16 Mix mechanism socket 4 /
P1 Built-in scanner socket 15 /

Indicators and test points

Indicators on the autoloader board
Indicators Description
D3 12V power indicator, lights when the 12V power supply works properly
D4 5V power indicator, lights when the 5V power supply works properly
D5 3.3V power indicator, lights when the 3.3V power supply works properly
D25 MCU working indicator, flickers at the frequency of 0.5s when MCU works
properly
D26 FPGA working status indicator, flickers at the frequency of 0.5s when FPGA
works properly

Autoloader board test points

No. Name Pins Description
1 TP1 Pin3 of X1 The output signal of 24.4545M crystal oscillator
clock, FPGA work clock
2 TP2 PIN 47 of U2 RD signal from MCU
3 TP3 PIN 62 of U2 WR signal from MCU
4 TP4 PIN 0 of U2 ALE signal from MCU
5 TP5 PIN 28 of U2 FPGA reset signal
6 TP6 PIN 5 of J1 FPGA configuration start signal
7 TP7 VCC +5VDC voltage (5±0.25V)
8 TP8 12V0 +12VDC voltage (+12±1.2V)
9 TP9 24V0 +24VDC voltage (+24±1.2V)
10 TP10 VDD +3.3VDC voltage (+3.3±0.15V)
11 TP11 GND
12 TP12 GND
13 TP13 1V5 +1.5VDC (1.5±0.05V)
14 TP15 TUBE Tube detection sensor output signal (> 0.4V when
blocked, < 0.2V when not blocked)
15 TP18 PIN 3 of X2 The output signal of 24.4545M crystal oscillator
clock, MCU work clock
16 TP21 PIN 12 of Feeding mechanism Y motor stepping pulse signal
U16
17 TP23 PIN 12 of Feeding mechanism X motor stepping pulse signal
U18
18 TP24 PIN 12 of Feeding mechanism Z motor stepping pulse signal
U22

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Hardware System

19 PIN 12 of
TP25 Mix mechanism X motor stepping pulse signal
U23
20 PIN 12 of
TP26 Mix mechanism stepping pulse signal
U24

Troubleshooting
The autoloader board uses mature design and is very unlikely to break down. For the errors of the
board itself, refer to the troubleshooting of the drive board for solutions. Here we only list some
common errors occurred during the autoloading process and their corresponding troubleshooting
information.
Troubleshooting for Autoloading Module

No. Error type Cause Troubleshooting Note

1. Use a multimeter to measure the

voltage between the black and white
wires at the sensor ends. When it is
powered on and the compartment
When the
The position door is closed, the voltage should be
compartment
sensor for the larger than 3V; open the door
door cannot
door manually and measure the voltage
open, it is
compartment again, the value should be less than
most
door failed, or 0.2V. If the voltages are out of such
Door open probably
1 the ranges, the sensor is broken, please
error caused by the
electromagnet replace it.
failure of the
encounters too 2. When the sensor works properly,
door
much resistance press the [OPEN] key again, and
mechanism
and cannot be check whether the electromagnet
and the
drawn out. moves. If the electromagnet moves
spring.
but still the compartment door won't
open, then the door mechanism has
failed. Remove and re-install the
door spring, or replace the spring.
Make sure current setting for the scanner
Wrong scanner (code system and digits) is in consistent
settings; with the used barcode; check whether
unqualified the barcode is stuck at the proper
Failed to
barcode sticking; position (at least 16mm from the bottom
2 scan the /
poor barcode of the tube); check whether the mirror on
barcode
quality; scanner the scanner is blocked by dust or other
installed at objects; check the barcode quality,
wrong position. whether there is any stain or notch; or
whether the contrast ratio is sufficient.

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Hardware System

Y-direction left or
right sensor fails; 1. Check whether the sensor works
the Y-direction properly;
Y-direction
sensor block 2. When the sensor works OK, then the
3 left or right /
sheet does not error must be caused by the sensor block
sensor error
move correctly sheet. Adjust or replace the sensor block
with the tube sheet.
feeding.
The error is
reported when
1. Check whether the micro-switch is
the X-direction
pressed down; Pay special
loading should
2. When it is pressed down, check attention to
have been in
X- direction whether the switch works effectively. the
position but the
4 loading When the micro-switch is pressed down, micro-switch
system cannot
failed the voltage between its two ends should and its
detect the
be 0V; when it is not pressed down, the connection
loading in-
voltage measured should be larger than wires.
position signal
3V.
from the
micro-switch.

4.5.4 Preamplification Board

Function overview
The preamplification board amplifies and quantifies the weak photoelectric signals produced by the
photodiode, and transfers them into digital signals for output. In this way, it realizes the
measurement of weak analog signals. The board is connected with the drive board, from which it
was supplied with power and to which it outputs the measurement results.
Structure
The preamplification board consists of the analog part and the digital part. Its structure is shown in
Figure 4-7:

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Hardware System

Figure 4-7 Preamplification board

Only one of the metallic oxide apertures (the one marked MH4 on the up left of Figure 4-7) is
connected to AGND.

Socket Description
The preamplification board has only one socket, whose pins are defined as below:
Table 4-9 Socket pins on the preamplification board
Position no. No. Functions Description
Digital circuit
J1 Enter
1 ground
Digital power
J1 2 Output 5V
circuit
J1 3 ADC output Output
J1 4 ADC clock Input
Digital circuit
J1
5 ground Input
ADC chip
J1 6
select Input
J1 7 Analog circuit Output 5V
Analog
J1 8
ground Input
J1 9 Not used NC
Analog
J1 10
ground Input
J1 11 Analog power Output -12V
J1 12 Analog power Output 12V

Indicator Description
None

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Hardware System

Test Points
Table 4-10 Test points on the preamplification board
Test
Definition Description
Points
ADC reference Reference range [4.9V, 5.1V]. Voltage out of that range
TP1
voltage indicates that the power chip AD586 may be damaged.
Square wave signal from the drive board, normally with a
frequency higher than 800KHz. When the signal
TP2 ADC clock signal frequency is too low, or there is no such signal received,
then the drive board or the drive chip
CY74FCT2245CTQCT may be damaged.
Analog signal test Reference range [4.9V, 5.1V]. Voltage out of that range
TP3
point indicates that the OPA chip may be damaged.
Analog signal test Reference range [-1V, 5V]. Voltage out of that range
TP4
point indicates that the OPA chip may be damaged.
Analog signal test Reference range [-1V, 5V]. Voltage out of that range
TP5
point indicates that the OPA chip may be damaged.
Analog signal test Reference range [-1V, 5V]. Voltage out of that range
TP6
point indicates that the OPA chip may be damaged.
Reference range [0.020V, 0.035V]. Voltage out of that
TP7 Reference voltage range indicates that the regulator chip
REF3033AIDBZTmay be damaged.
Square wave signal output from the preamplification
TP8 ADC output board. If no such output is detected, then the ADC may
be damaged.
Square wave signal from the drive board, normally with a
frequency higher than 300Hz. When the signal frequency
ADC chip select
TP9 is too low, or there is no such signal received, then the
input signal
drive board or the drive chip CY74FCT2245CTQCT may
be damaged.
Reference range [4.5V, 5.5V]. Voltage out of the range
TP10 ADC power indicates ADC damage or the short circuit of
preamplification board.
Reference range [-0.05V, 0.05V]. Voltage out of the
TP11 Analog ground
range indicates the short circuit of preamplification board.
Reference range [10V, 14V]. Voltage out of the range
TP12 12V analog circuit
indicates the short circuit of preamplification board.
-12V analog Reference range [-10V, 14V]. Voltage out of the range
TP13
circuit indicates the short circuit of preamplification board.
Digital circuit Reference range [-0.05V, 0.05V]. Voltage out of the
TP14
ground range indicates the short circuit of preamplification board.
Digital power Reference range [4.5V, 5.5V]. Voltage out of the range
TP15
circuit indicates the short circuit of preamplification board.

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Troubleshooting
Abnormal voltage
values on the "Gain"
screen

Yes
If the drive board
output voltage Drive board error
abnomral?

Perform background
or aging tests for 3
times

Yes
Error removed? Record

Yes
Check if the
Weld the MH6
welding spot MH6
spot
fell out?

Other errors

Figure 4-8 Troubleshooting of the preamplification board

The preamplification board rarely finds errors due to its simplified design; so before you start the
preamplification board troubleshooting procedure, check the analog circuits output by the drive
board (including ±12V, analog 5V and digital 5V) one by one against the information stated in above
Table 1, and make sure there is no error with the drive board voltages.
While the drive board output voltages are all normal, but the "Gain" screen displays abnormally low
voltage (<1V), run background (or aging) tests for 3 times to remove any bubble in the flow cell, and
then check the voltage values on the "Gain" screen again to see if they are normal.
If the error remains after all above steps (the voltages displayed on the "Gain" screen are
continuously lower than 1V or keep jumping), check if the welding spot MH6 between the
preamplification board and the PD has fallen out by pressing the area near the spot. If the gain
voltages shows sudden and large change (>2V), then the MH6 spot needs to be welded again.
If the possibility of all above errors is excluded during the troubleshooting procedure, you need to
find the error elsewhere.

4.5.5 LED Drive Board

Function Overview
The LED drive board transfers voltages to constant current and drives the LED to light. The board is

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Hardware System

connected with the drive board. The drive board supplies power to the LED board and controls its
switching.
Structure

Figure 4-9 LED drive board

The metallic oxide aperture (see Figure 4-9) is connected to AGND.

Socket Description
The LED drive board has two sockets: J1 connects to the drive board, and J2 connects to the LED.
Table 4-11 J1 socket pins on the LED drive board
Position
No. Functions Description
no.
J1 1 Analog power Output 12V
Switch control
J1 2 Input [0V-12V]
socket
J1 3 Analog ground Input

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Hardware System

Table 4-12 J2 socket pins on the LED drive board

Position
No. Functions Description
no.
LED drive Negative
J2
1 socket terminal
LED drive Positive
J2 2
socket terminal

Indicator Description
N/A

Test Points
Table 4-13 LED drive board test points
Test
Definition Description
Points
When the LED is connected and the LED light is on,
TP1 Switch test point the voltage should be lower than 0.5V. Otherwise the
switch tube IRLML2502PBF may have error.
When the LED is switched on, the voltage detected at
Switch control this test point should be higher than 3V; and when the
TP2
test point LED is switched off, the voltage should be <0.5V.
Otherwise the drive board may be with error.
When the LED is switched on, the voltage should be
Reference within the range of [0V, 2.5V]. When it is out of the
TP3
voltage test point range, then the power chip ZR431F01TA may be
damaged.
Reference range [0V, 0.05V]. Voltage out of the range
TP4 Analog ground
indicates the short circuit of LED drive board.
When the LED is switched on, the voltage should be
Reference within the range of [0V, 2.5V]. When it is out of the
TP5
voltage test point range, then the power chip ZR431F01TA may be
damaged.
Reference range [11V, 13V]. Voltage out of the range
TP6 Analog circuit indicates the damage of the drive board or the short
circuit of LED drive board.
When the LED is switched on, the voltage should be
Digital power within the range of [2.5V, 12V]. When it is out of the
TP7
test point range, then the power chip ZR431F01TA may be
damaged.

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Hardware System

Troubleshooting

LED does not light

Yes
If drive board
Drive board
output voltage
error
abnormal

Short circuit TP1

and TP4

Drive board does

not send out
Yes
signals to switch
Error removed? on the LED, or the
switch tube
IRLML2502PBF
No is damaged

Adjust the
potentiometer to
its two extreme
values

Yes
Error removed? Record

Other errors

Table 4-14 Troubleshooting of the LED drive board

The LED drive board rarely finds errors due to its simplified design; so before you start the LED drive
board troubleshooting procedure, check the analog circuits output by the drive board one by one
against the information stated in above Table 4-11, and make sure the drive board voltages are all
normal.
If all drive board output voltages are normal, short circuit TP1 and TP4. If doing this may make the
LED work properly, then either the drive board did not send out signals to switch on the LED, or the
switch tube is damaged. If the LED still does not light on, there is the possibility that the current
supporting the LED is too weak, and the LED light is too feeble to be recognized. Try adjusting the
potentiometer to its two extreme values.
If the possibility of all above errors is excluded during the troubleshooting procedure, find the error
elsewhere.

4.5.6 Liquid Pressure Detection Board

Function Overview
Liquid pressure detection board (clog detection board) detects the pressure around the flow cell,

4-25
Hardware System

which is then transmitted in the form of the liquid pressure signals to ADC through the liquid pressure
sensor. The signals are then transferred to digital signals and transmitted to data board through
serial port transmission.

Figure 4-10 Liquid pressure detection board structure

Socket Description
Position Description
No. Functions
no.
1 J1 The socket connects the liquid pressure /
sensor which receives liquid pressure
signals
2 J4 The interface connects data board. It
supplies power and works as the serial
port to transmit the digital signals of
liquid pressure to data board.

Indicator Description
Indicator Definition functions
s
The LED indicator stays on under normal conditions
D6 5V voltage indicator When the indicator is off, the 5V voltage is abnormal, and
you need to check if the 12V voltage is OK.
D7 12V power supply The LED indicator stays on under normal conditions

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Hardware System

indicator When the indicator lights off, then the digital 12V power
supply to the liquid pressure detection board is with error.
Check whether the cables connecting the liquid pressure
detection board and the data board are properly plugged,
or the digital 12V voltage of the data board is normal.

4.5.7 Touch Screen Control Board

Function Overview
The touch screen control board, by connecting to the digital main control board on one end, and to
the 4-wire resistive touch screen on the other end, can be seen as the bridge between the touch
screen and main control board. It converts the touch screen output signals to digital signals
recognizable by the main control board, and the digital signals later are transferred to position
signals by main control board CPU.
Structure

Table 4-15 Touch screen control board

Sockets and indicators

Table 4-16 Sockets and indicators on the touch screen control board

No. Position no. Functions Description

1 J1 Touch screen socket Receive touch screen signals
2 J3 Communication socket to main Enable the communication between
control board the touch screen control board
power circuit and the main control
board
3 D5 3.3V power indicator \

4-27
Hardware System

Troubleshooting
Table 4-17 Troubleshooting of the touch screen control board

No. Error description Troubleshooting

1 The touch screen is damaged 1. Check whether the J1 and J4 cables are
properly inserted;
2. Check whether the D5 indicator stays on;
3. When the above two points are OK, unplug
and reconnect the J1 cable, and switch off and
re-electrify the touch screen control board.
4. If the error remains, replace the touch screen
or the touch screen control board.

4.5.8 Indicator Board

Introduction
The indicator board connects to the digital main control board. It receives the control signals output
by the digital control board, indicates the whole device status and sends out sound alarms.
Functions
The indicator board has two major functions:
Status indication function: the indicator board uses the 3-color indication system (red, yellow and
green) to indicate different status of the whole device.
Sound alarm function: the buzzer on the indicator board will sound alarm when errors are detected.
Structure

Indicator board insert diagram (front)

Indicator board insert diagram (back)

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Hardware System

Sockets
There is one socket (J1) on the indicator board. It is defined as below:
Definition of J1 socket
PIN Signals Description
It sends out signals to control the green indicator, which will
1 GREEN_LED light on when high level signals are received, and light off when
low level signals are received.
It sends out signals to control the yellow indicator, which will
2 YELLOW_LED light on when high level signals are received, and light off when
low level signals are received.
It sends out signals to control the red indicator, which will light
3 RED_LED on when high level signals are received, and light off when low
level signals are received.
4 GND Digital circuit ground
It sends out signals for the buzzer, which will sound when high
5 BUZ_N level signals are received, and remain silent when low level
signals are received.
6 GND Digital circuit ground
7 VCC 5V power circuit, supply power to the buzzer and the indicators
8 \ Reserved design

Indicators and test points

The test points on the indicator board are defined as below:
Indicator board test points
Test
Silk-screen Function Description Note
Points

TP1 VCC Test point of 5V power Normal voltage range 4.75V-5.25V

TP2 GND Ground test point Normal voltage range 4.75V-5.25V

Green indicator control signal

TP3 / 3.3V TTL level
test point
Yellow indicator control signal
TP4 / 3.3V TTL level
test point
Red indicator control signal
TP5 / 3.3V TTL level
test point
When the indicator lights on, a low
Green indicator working electrical level should be detected,
TP6 /
status test point and when it lights off, a high level
of 5V should be detected.
Yellow indicator working When the indicator lights on, a low
TP7 /
status test point electrical level should be detected,

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Hardware System

and when it lights off, a high level

of 5V should be detected.
When the indicator lights on, a low
Yellow indicator working electrical level should be detected,
TP8 /
status test point and when it lights off, a high level
of 5V should be detected.

Troubleshooting
There are two common errors often found with the indicator board:
 Indicators does not light properly
Possible causes: poor connection of the J1 cable to the J1 socket; or damage of the indicator;
To remove the error: unplug and re-connect the very cable; replace the cable; or replace the LED
indicator
 Buzzer does not work properly
Possible causes: poor connection of the J1 cable to the J1 socket; or damage of the buzzer;
To remove the error: unplug and re-connect the very cable; replace the cable; or replace the buzzer

4.5.9 Power Patching Board

Function Overview
051-001345-00 power patching board distributes and monitors the power board-output-voltages to
various boards, and indicates the power board status through indicators.

Figure 4-11 Power patching board

4-30
Hardware System

Socket description

Position no. Functions

J1 Not used
J2 Output 5V and 12V digital power circuit for the main control board
Output ±12V analog power circuit for the analog drive board

J3 Supply power to the analog drive board

J4 Not used
J5 Supply power to the autoloader board
J6 Supply power to the analog drive board
J7 Supply power to the autoloader board

Indicator Description

No. Indicator position number Description

1 D1(D5V) Digital 5V indicator: when it lights on, the digital 5V
power circuit output by the power supply module
works OK; otherwise, it is with error.
2 D2(D12V) Digital 12V indicator: when it lights on, the digital 12V
power circuit output by the power supply module
works OK; otherwise, it is with error.
3 D3(P12V) Power 12V indicator: when it lights on, the power 12V
power circuit output by the power supply module
works OK; otherwise, it is with error.
4 D4(P24V) Power 24V indicator: when it lights on, the power 24V
power circuit output by the power supply module
works OK; otherwise, it is with error.
5 D5(A12V) Analog 12V indicator: when it lights on, the analog 12V
power circuit output by the power supply module
works OK; otherwise, it is with error.
6 D6(A12V_N) Analog -12V indicator: when it lights on, the analog
-12V power circuit output by the power supply module
works OK; otherwise, it is with error.
7 D7(A5V7) Analog 5.7V indicator: when it lights on, the analog
5.7V power circuit output by the power supply module
works OK; otherwise, it is with error.

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Hardware System

4.5.10 RFID Card Reader

Function Overview
023-000836-00 RFID card reader is the interface to the RFID label. It reads and writes into the RFID
label at the bottom level.

Figure 4-12 RFID card reader

Socket Description
Table 4-18 Sockets to the RFID card reader

Sockets Functions Note

J6 Communication socket to main control board /
J3 Power enable socket /

Indicator Description
There is only one red running indicator on the RFID card reader (as marked on above figure).
1. The indicator flickers only once when the card reader completes POST;
2. and stays on when the card is read or written into.

4.5.11 Display Screen Assembly

Function Overview
The display screen assembly mainly consists of the LCD display screen and the cables. It is the user
interactive interface.

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Hardware System

Structure

Figure 4-13 Display screen assembly

Troubleshooting of common errors

Table 4-19 Troubleshooting of display screen assembly

No. Error description Troubleshooting

1 The display screen does not light on Check whether the connection between the
backlight socket and the screen signal cable
fells loose.
2 Touch screen displays poor color Check whether the connection between the
signal socket and the screen signal cable
fells loose.
3 The touch screen is damaged /
4 Compartment door cannot be opened Check whether the keyboard signal cable is
properly connected, or any of the keys is
damaged
5 Buzzer does not sound or indicator does /
not light

4.5.12 Key Board

Function Overview
When the key is pressed down, the switch is coupled; and when the main control board detects the
action, it sends out a command to open the compartment door. We re-use the same SM&S printing

4-33
Hardware System

module key board here. And the PCB is only for installation and for lead wires.
Structure

When the key board is pressed down, the two pins of J1 are connected.
Socket Description
Socket Function Note
J1 The socket connects to the main control board. When /
the key board is pressed down, the two pins of J1 are
connected.

4.5.13 Power Supply Assembly

Function Overview
The power supply assembly transfers AC circuit to DC circuit, and supplies power to the boards
inside the analyzer.
Structure
The power supply assembly mainly consists of the power input socket, the power switch, the power
supply and the radiator fan. The power input socket inputs and filters the power. The connecting and
disconnecting of power are completed through the power switch. Make sure you have unplugged all
power cords before you service the power supply.

Power
input Power
socket patching
board joint

GND

Power
switch

4-34
Hardware System

Service Instructions
The power supply assembly is always serviced and replaced as a whole.
1. When power supply assembly error is suspected, unplug all the cables to the power patching
board first. Check the indicators on the power patching board, or use a multimeter to measure
the voltages. If no voltage or abnormal voltage is detected, then the power patching board is
damaged. Replace the power supply assembly.
2. When the board is powered on, but the fan does not work, and there is no voltage output,
dissemble the power supply assembly and check whether the fan is connected correctly. Use a
multimeter to measure the voltage on power input socket and see if normal AC voltage can be
detected. If there is no such problem, replace the power supply assembly.

4.6 Service Instructions for Other Parts Closely-Related

To Hardware
Such parts include sensors, air pressure sensor, liquid pressure sensor, temperature sensor,
degasser and rotary feeding valves etc.

4.6.1 Sensors
The H50/H50P analyzer uses two outsourced transmissive sensors: the 4-wire transmissive sensor
(ID: 801-3003-00015-00) and the 3-wire sensor. The difference between the 4-wire sensor and
3-wire sensor is that the power supply wire and signal wire of the 3-wire sensor share one earth wire,
while those of the 4-wire sensor have their own earth wires.

Figure 4-14 4-wire transmissive sensor 3-wire transmissive sensor

The 3-wire sensor has 3 wires: red, blue and black, while the red one is the power supply wire, black
the earth wire, and the blue one signal output wire. The voltage between the red wire and black wire
is fixed to be (4.75-5.25)V. When the optical path is blocked, the voltage between the blue wire and
black wire is about 3.3V, and when not blocked, the voltage will be less than 0.2V.
The 4-wire sensor has 4 wires: red, black, green and white, while the red one is the power supply
wire, the black one and green one are earth wires, and the white one signal output wire. The voltage
between the red wire and black wire is fixed to be (4.75-5.25)V. When the optical path is blocked, the
voltage between the white wire and green wire is about 3.3V, and when not blocked, the voltage will
be less than 0.2V.

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Hardware System

Common errors related to digital output sensors include:

1) External light interference
Avoid direct exposure to sunlight; otherwise, the sensor may not work properly.
2) Connecting cable damaged
The connecting cable of the sensor may be damaged in the assembly process on the manufacture
site or in the first installation and adjustment. If the power supply wire or signal wire is damaged and
the earth wire is short-circuited, the sensor will lose efficacy. To remove this type of error, bind up the
breakage or replace with a new sensor cable.

4.6.2 Hydraulic Sensor

The H50/H50P analyzer has two hydraulic sensors, one of which is directly connected to the analog
drive board for liquid pressure detection; and the other is connected to the clog detection board,
which is then connected to the main control board, for back pressure detection.
Frequent errors found in liquid pressure detection:
1. Check whether the connecting cables of the liquid pressure detection board and the drive board
are damaged or broken. If yes, replace the cables;
2. The connection between the cables and the board end gets loose. Secure the connector;
3. Check whether the drive board is burnt;
If the error still remains after all above steps, the sensor may have error, replace the liquid pressure
sensor.
Frequent errors found in back pressure detection
1. Check whether the connecting cables of clog detection board and the main drive board are
damaged or broken. If yes, replace the cables.
2. The connection between the cables and the boards end gets loose. Secure the connector;
3. The connector on the clog detection board end gets corrosive by liquid leakage. Replace the
connector.
If the error still remains after all above steps, the sensor may have error, replace the liquid pressure
sensor.

4.6.3 Temperature Sensors

There are in all five temperature sensors. One is for ambient temperature detection, two for the
column thermostatic chamber temperature detection, and two for optical flow cell temperature
detection.
Abnormal ambient temperature: check whether the ambient temperature sensor or the cables are
broken;
Abnormal temperature of column thermostatic chamber and optical flow cell: when the temperature
screen displays 50℃, first check whether the cables are properly connected, and then check
whether the temperature sensor is short circuited; when the temperature screen displays 0℃, or the
value keeps jump violently, check whether the temperature sensor gets short-circuited, or the sensor
and the cables are broken.

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Hardware System

4.6.4 Degasser
The degasser errors can be detected by degasser status register. When the degasser reports
abnormal status, first check whether the degasser status cable is properly connected. If yes, check if
the degasser is working, and if it is not working, check whether the power cord of the degasser is
connected. If the degasser is working but cannot reach the target pressure, check whether there is
any leakage on the degasser and the tubing. If the error remains after all above steps, replace the
degasser.

4.6.5 Rotary Feeding Valve

There are two major errors relating to rotary feeding valve.
1. The rotary feeding valve does not move: check whether the power and control cables are
properly connected. Unplug the power cord of the valve and reconnect it to see if it may move. If
not, then the valve is damaged.
2. The rotary feeding valve cannot rotate to target position: check whether the control cable of the
rotary feeding valve gets loose. Then unplug the power cord of the valve and reconnect it. If the
valve just clicks, it is OK; if it rotates for 1 or 2 seconds, then it is damaged.

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Chapter 5 Software

5.1 Introduction
This chapter describes the operation methods of those software functions possibly used by the
service engineer when installing and maintaining the analyzer. (Those operations contained in
the user manual are excluded from this chapter.) This software should be operated with
reference to the service manual or production process of other technical teams (like hardware,
fluidics).
This chapter contains the following contents:
Part 1: Introduction
Part2: Password
Part3: Functions of “Service” access level
Part4: Analyzer Update
Part5: LIS Setup

5.2 Password

User Access Level Definition

There are 5 access levels: Operator, administrator, service engineer, manufacturer and R&D
engineer. Users in different levels are only allowed to access some particular functions.

Table 5-1 Functions of the Access Levels

Functions of Level 1 Functions of Level 2 Minimum

Authority
User Management
Add user administrator
Change Password Operator
Delete user administrator
Startup & Shutdown
Login Operator
Logout Operator
Shut Down the analyzer Operator
Packup & Shutdown administrator
Sample Analysis Operator
Results Review
Table Review Operator
Graph Review Operator
Software

View Results Operator

Validate/Cancel Val. administrator
Delete sample administrator
Print Result Operator
Communicate Result Operator
Export Result Operator
Review special info. administrator
View patient information Operator
Edit patient information Operator
QC Analysis
View QC files Operator
Modify QC files administrator
Clear QC files administrator
View QC graph Operator
View QC table Operator
Delete QC sample administrator
Print QC result Operator
Communicate QC result Operator
Export QC result Operator
Analyzer Calibration
AL count calibration administrator
Adjust calibration factor administrator
View the calibration history administrator
Export the calibration history administrator
Touch screen calibration administrator
Performance test
Repeatability Test Service engineer
Carryover Test Service engineer
Aging test Manufacturer
Setup
Set up conditions of time-based administrator
maintenance

Set up System Time administrator

Set up Barcode Types administrator
Select Parameter Unit administrator
Set up Reference Ranges administrator

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Select report parameter administrator

Set up Department and Shortcut administrator
code
Set up print parameters administrator
Set up communication administrator
parameters
Set up fluidic volume parameters Service engineer
(Fluidic Volume Calibration)
Set up temperature parameters Service engineer
(Temperature Calibration)
Set up Pressure parameters Service engineer
(Pressure Calibration)
Set Optical Channel Gains administrator
Set Analyzer Serial No. Service engineer
Set analyzer configuration Service engineer
Set up language Service engineer
Maintenance
View Logs administrator
Export Logs administrator
View Errors Operator
Remove Error Operator
Load column information administrator
Replace the reagents Operator
Wake up the analyzer Operator
Empty Service engineer
Prime Service engineer
Probe Cleanser maintenance Operator
Analyzer Debug
Self-test Service engineer
Adjustment Service engineer
Sequences debug R&D engineer
Export Debug Data Service engineer
View Status
View Analyzer Status during Operator
Sample Analysis
View Analyzer Run Status Service engineer
View vulnerable-parts statistics Service engineer

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View version information Operator

Analyzer Update Service engineer

Preset User Information

Table 5-2 Preset User Information
User name Name Authority Password
Admin Administrator administrator Admin
service Null Service engineer Se s700
Pro*（Note 1） Null Manufacturer /
RD Null R&D engineer /
Note1: * is the manufacturer code which should be input with the prefix Pro together. It consists
of 1~9 numbers.

Add user
This function allows you to add users. You may add up to 30 operators and 20 administrators.
The following user information can be edited:

Table 5-3 User Information

Inspection Type Specification

Item
User name character 1 to 12 characters in length, including letters,
string numbers and the following characters:
~@#%-_+={}[], not case sensitive
Name character 0 to 20 characters in length, including letters,
string numbers and the following characters: ：
~`!@#$%^&*()-_+={}[]\|:;”’,./<>?, case sensitive
Password character 0 to 12 characters in length, including letters,
string numbers and the following characters: ：
~`!@#$%^&*()-_+={}[]\|:;”’,./<>?, case sensitive
Access level options Operator/Administrator

Table 5-4 Add user

Access level User types allowed to add

Operator N/A
administrator administrator, Operator
Service engineer administrator, Operator
Manufacturer administrator, Operator
R&D engineer administrator, Operator

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Software

Delete user
This function allows you to delete users. Particular types of users can be deleted except the
current user logged in.

Table 5-5 Delete User

Access level User types allowed to delete

Operator N/A
administrator administrator, Operator
Service engineer administrator, Operator
Manufacturer administrator, Operator
R&D engineer administrator, Operator

5.3 Functions of Service Access Level

5.3.1 Menu Structure Overview

Table 5-6 Menu Structure

Level 1 menu Level 2 menu Level 3 menu

Sample Analysis
Table Review
QC Setup
Graph
Table
Calibrate Manual
Calibrator
Touch Screen Calibration
Performance Aging
Repeatability
Carryover
Setup Date/time
User Management
Parameter
Shortcut Code
Auxiliary
Maintenance
Barcode Setup

5-5
Software

Print Setup
Communication
Gains
Advanced Setup
Maintenance Consumables Reagents
Analytical Column
Replacing Reagents
Empty and Prime
Fluidics
Sequences debug
Log
Service Temperature
Pressure
Fluidic Volume
Self-test
HPLC Self-Test
Debug Sample Probe
Barcode scanner
Debug data export
Status Statistics
Sensor Floater
Sensors
Others
Temperature and
Pressure
Voltage
Version information
Logout
Shutdown

5.3.2 Repeatability test

Function Entry
Performance -> Repeatability

5-6
Software

Screen

Instruction for Use

1. Load the PD Tube Rack and tap [Start].
2. The analyzer loads the PD Tube Rack and detects the tubes. When a sample is found, it will
begin to test.
3. When a single sample test is finished, the analyzer will show the result of the repeatability
test sample. The latest test result will be showed preferentially.
4. The analyzer will automatically select those samples that meet the requirement of the
sample group of repeatability test, and update the number of the selected samples. When
the number of the selected samples reaches the minimum number required by the sample
group, the analyzer will show the repeatability index and test result. When the test result is
"FALL", the analyzer will highlight the result in red.
5. Use the navigation buttons to browse the samples of repeatability test in the list. You may
browse the previous/next sample, previous/next page or the first/last sample by tapping the
arrow buttons on the left of the screen.
6. Change the selected status of the samples.
7. The analyzer updates the number of the selected samples, the repeatability index and the
test result.
Note
2a. When the analyzer detects non-AL PD Tube Rack, the prompt "Rack Type Error" will show
up and the test will be stopped.
4a. When the number of the selected samples does not reach the minimum number required,
the analyzer will not perform the calculation of repeatability index, nor give the test result.
4b. When the sample does not meet the sample group requirement of repeatability test, it

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cannot be selected.

5.3.3 Carryover test

Function Entry
Performance -> Carryover
Screen

Instruction for Use

1. Load the PD Tube Rack and tap [Start].
2. The Analyzer loads the PD Tube Rack and detects the tubes. When a sample is found, it will
begin to test.
3. When a single sample test is finished, the analyzer will show the results of the carryover test
samples according to the sample match sequence.
4. When the test of the carryover samples is finished, the analyzer will show the carryover
index and the test result. When the test result is "FALL", the analyzer will highlight the result
in red to remind the user.
Note
2a. When the analyzer detects non-AL PD Tube Rack, the prompt "Rack Type Error" will show
up and the test will be stopped.
3a. When the sample result cannot meet requirements, the analyzer will remind the user that
the result is invalid, and it will empty the test results of the carryover samples, and stop
testing.

5-8
Software

5-18
Software

Screen

Instruction for Use

1. Select a certain item under the Status menu.
2. The analyzer will show the current status of the selected item.
Note
N/A

5.3.15 View vulnerable parts info.

Function Entry
Status->Statistics

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Software

Screen

Instruction for Use

1. Enter the Statistics screen to view the Used Times of the vulnerable parts; you may initialize
the Used Times.
2. When the Used Times of the vulnerable parts has reached the Max. Uses, the analyzer will
remind you the error of "Uses out of limit".
Note
N/A

5.4 Analyzer Update

5.4.1 Notes before Update

Update Times
It will take about 5~8 minutes to finish the complete software update.
It will take about 1~3 minutes to finish the complete hardware MCU and FGPA update.
Please be patient to wait for the updating result. Although the progress bar may remain
immobile in a short time, do not turn off the power supply, otherwise the update may fail or the
analyzer cannot be started.

Troubleshooting in the Update Process

If the analyzer prompts the information of mount failure and the prompt “Connect the USB
flash drive", please insert the USB flash drive again and wait 10 seconds before restart the

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update. You may try multiple times. If the prompt "Connect the USB flash drive" keeps showing,
please change another USB flash drive.
If the update package includes boot software, hardware MCU and FPGA, when the analyzer
prompts update failure, please do not restart the analyzer or turn off the power supply.
Ensure the correctness of the USB flash drive and update package before restart the update.
If unexpected power outage happens when updating other items, please power on the
analyzer again, and the updating module will run automatically when the analyzer is restarted.
Note: Under the circumstance of update unfinished or failed, the analyzer will automatically run
the update program after being restarted. Please do not tap the "Cancel" button to give up
update. Otherwise, even if the previous update operation has made the analyzer software in
an uncertain situation, the update program will not run again, and the analyzer cannot be
started.

Do not pull off the USB flash drive during the update process

5.4.2 Update Steps

Step 1: Update package preparation
1. Run the Windows operating system;
2. Insert the installation disk of the analyzer into the DVD ROM of the PC, and open the disk in
"My Computer";
3. Find "update.rar" in the disk, extract "update.rar" to the update folder, copy update folder to
the USB flash drive, and then insert the USB flash drive to the USB interface on the right
side of the analyzer.

Step 2: Updating
1. Insert the SD card to the card slot of the analyzer, and then turn on the analyzer. When the
touch screen calibration is finished, you will enter the software screen, as the following
figure shows:

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Software

Figure 5-1 Application Software Screen

2. Tap "Status->Version Info." in the menu bar to enter the update screen, as the following
figure shows:

Figure 5-2 Version Information Screen

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Software

3. Tap "Start Update" button on the Version Information Screen to enter the Version Update
screen, as the following figure shows:

Figure 5-3 Update Screen

4. Tap "Update" button to start the update process. Firstly the prompt box "Preparing for
Update" pops up, as the following figure shows:

Figure 5-4 Prompt Box of Preparing for Update

5. The preparing process for update generally lasts for 10 seconds or, at most 1 minute. On
the screen, “……”will keep showing up, please be patient. When the preparation is done,
the prompt box of update confirmation pops up, as the following figure shows:

Figure 5-5 Prompt Box of project information and disk version (The actual version information

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Software

shall prevail.)

6. Tap "Yes" button to start the update process. Firstly, the prompt box of File Verification
pops up, as the following figure shows:

Figure 5-6 Prompt Box of File Verification

7. When the file verification is finished, the File Update Screen pops up. After the file update
is finished, the update of application software will get to start. After that, the update of
hardware driver will follow closely. The following figure shows the prompt box of
application software update.

Figure 5-7 Prompt Box of application software update

Note: When updating the hardware drive, the prompt "Updating the drive board" will pop up. If
some item is not updated, the corresponding update prompt will not be displayed.

8. Data update will be performed then, as the following figure shows:

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Software

Figure 5-8 Prompt Box of Updating data

9. When the data update is finished, the prompt “Update succeeded!" pops up, as the
following figure shows:

Figure 5-9 Prompt Box of Updating success

10. Tap "OK" button, and the prompt "Please turn off the analyzer and restart" will pop up, as
the following figure shows:

Figure 5-10 Prompt Box of Restarting the analyzer

11. Turn off the analyzer, pull off the USB flash driver and wait for a while, like 20 seconds, and

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Software

then turn on the analyzer again.

12. When the startup process is over, tap the Menu button on the upper left corner of the main
screen to display the submenus, and then tap "Status"--"Version Information" to check if
the updated version is correct.

5.5 LIS Setup

Function Entry
Setup -> Communication

Screen

5.6 Error Code

0x01000101 Waste container full The waste container is full. Replace it with a new
one. Connect a floater even when the waste is
discharged directly.
0x01000102 No Eluent A When the error is reported, the user will be prompted
to install a new one; and the analyzer will stop
analysis if the user does not do so. If the analyzer
still does not respond after a new reagent is
installed, try to move the reagent bag a little.

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Software

0x01000103 No Eluent B When the error is reported, the user will be prompted
to install a new one; and the analyzer will stop
analysis if the user does not do so. If the analyzer
still does not respond after a new reagent is
installed, try to move the reagent bag a little.
0x01000104 No Hemolysis When the error is reported, the user will be prompted
Solution to install a new one; and the analyzer will stop
analysis if the user does not do so. If the analyzer
still does not respond after a new reagent is
installed, try to move the reagent container a little.
0x01000105 Please replace If the user has not installed a new reagent in 5
Eluent A minutes after the message appeared, the analyzer
will stop analysis.
0x01000106 Please replace If the user has not installed a new reagent in 5
Eluent B minutes after the message appeared, the analyzer
will stop analysis.
0x01000107 Please replace lyse If the user has not installed a new reagent in 5
minutes after the message appeared, the analyzer
will stop analysis.
0x01000108 Eluent A expired Eluent A is expired. The problem will not forbid the
user operation for the moment.
0x01000109 Eluent B expired Eluent B is expired. The problem will not forbid the
user operation for the moment.
0x0100010A Hemolysis Solution Hemolysis Solution is expired. The problem will not
expired forbid the user operation for the moment.
0x0100010B Hemolysis Solution Never appear
cistern is empty
0x0100010C Eluent A cistern is Never appear
empty
0x01000112 Replacing Eluent A The error will be reported when a valid RFID label is
failed detected, but the aspiration of the reagent fails due
to poor tube connections.
0x01000113 Replacing Eluent B The error will be reported when a valid RFID label is
failed detected, but the aspiration of the reagent fails due
to poor tube connections.
0x01000114 Failed to replace The error will be reported when a valid RFID card is
Hemolysis Solution detected, but the aspiration of the reagent fails due
to poor tube connections.
0x01000150 Column information The error may occur during the installation. Install a
loading failed new column as instructed, and the error will be
removed.

0x01000151 Column expired The RFID card of the column bears the expiration
date and the "Use before" date of the column. The

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Software

problem will not forbid user operation for the

moment.
0x01000152 Column uses out of The uses of the column exceeds the upper limit of
limit 3000 times, and further operation will be forbidden.
The use limit information is stored in the RFID card.

0x01000201 Column pressure If the analyzer keeps detecting high column

exceeds upper limit pressure, it will stop the analysis. When the error
appears, perform "Probe Cleanser Maintenance". If
the error still remains after that, replace the column.
0x01000202 Pressure out of When the error is reported, the analyzer will stop
upper limit analysis immediately. Check if there is any tubing
getting clogged.
0x01000203 Pressure out of When the error is reported, the analyzer will stop
lower limit analysis immediately. Check if any tube, especially
the fittings and tubes connected to the column get
loose.
0x01000204 Pressure No need to address it for now
building-up overtime
error
0x01000210 Optical flow cell No need to address it for now
temperature out of
upper limit
0x01000211 Optical flow cell Check if the cover of the optical system is closed
temp. out of lower
limit
0x01000212 Optical flow cell The temperature sensor of the optical system is
temperature reading damaged or not connected properly. The analyzer
error will stop the heating of the optical system.
0x01000220 Column No need to address it for now
temperature out of
upper limit
0x01000221 Column No need to address it for now
temperature out of
lower limit
0x01000222 Column The temperature sensor of the column is damaged
temperature reading or not connected properly. The analyzer will stop the
abnormal heating of the column.
0x01000230 Front cover illegally The analysis will be stopped immediately. It takes a
opened long time and lots of reagent to remove the error.
0x01000240 415nm blank The 415nm blank voltage signal out of upper limit.
voltage signal out of Check if there is any bubble in the flow cell. If there is
upper limit not, reset the gains of the optical system.
0x01000241 415nm blank The 415nm blank voltage signal out of lower limit.

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Software

voltage signal out of Check if there is any bubble in the flow cell. If there is
lower limit not, reset the gains of the optical system.
0x01000250 500nm blank The 500nm blank voltage signal out of upper limit.
voltage signal out of Check if there is any bubble in the flow cell. If there is
upper limit not, reset the gains of the optical system.
0x01000251 500nm blank The 500nm blank voltage signal out of upper limit.
voltage signal out of Check if there is any bubble in the flow cell. If there is
upper limit not, reset the gains of the optical system.
0x01000260 Buffer cistern No need to address it for now
pressure abnormal

0x01000301 Tube rack info. The card-reader cable under the bottom cover on
communication the right side of the analyzer is not connected
abnormal properly; or the board card is damaged.
0x01000302 Tube rack info. The label on the tube rack is damaged
reading abnormal
0x01000303 Eluent A info. The Eluent A card-reader cable is not connected
communication properly; or the board card is damaged.
abnormal
0x01000304 Eluent A info. The Eluent A RFID card is damaged.
reading abnormal
0x01000305 Eluent B info. The Eluent B card-reader cable is not connected
communication properly; or the board card is damaged.
abnormal
0x01000306 Eluent B info. The Eluent B RFID card is damaged.
reading abnormal
0x01000307 Hemolysis Solution The Hemolysis Solution card-reader cable is not
info. communication connected properly; or the board card is damaged.
abnormal
0x01000308 Hemolysis Solution The Hemolysis Solution RFID card is damaged.
info. reading
abnormal

0x01000401 Hemolysis Solution Syringe error. Check if the sensors are damaged,
syringe failed to and the motor may move smoothly.
leave home position
0x01000402 Lyse syringe failed Syringe error. Check if the sensors are damaged,
to return to home and the motor may move smoothly.
position
0x01000403 Hemolysis Solution Syringe error. Check if the sensors are damaged,
syringe failed to and the motor may move smoothly.
return to
sensor-blocked
area as expected

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Software

0x01000404 Hemolysis Solution Syringe error. Check if the sensors are damaged,
syringe is in blocked and the motor may move smoothly.
area
0x01000405 Lyse syringe No need to address it for now
command error

0x01000501 Sampling syringe Sampling syringe error. Check if the sensors are
failed to leave home damaged, and the motor may move smoothly.
position
0x01000502 Sampling syringe Sampling syringe error. Check if the sensors are
failed to return to damaged, and the motor may move smoothly.
home position
0x01000503 Sampling syringe is Sampling syringe error. Check if the sensors are
not in blocked area damaged, and the motor may move smoothly.
0x01000504 Sampling syringe is Sampling syringe error. Check if the sensors are
in blocked area damaged, and the motor may move smoothly.
0x01000505 Sampling syringe No need to address it
command error

0x01000601 Sample probe failed /

to leave home
position in vertical
direction
0x01000602 Sample probe failed /
to return to home
position in vertical
direction
0x01000603 Sample probe failed /
to leave home
position in
horizontal direction
0x01000604 Sample probe failed /
to return to home
position in
horizontal direction
0x01000605 Sample probe failed /
to find notch
0x01000606 Sample probe /
arrived at wrong
notch

0x01000701 Right edge of lyse /

position notch

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Software

overrun
0x01000702 Left edge of lyse /
position notch
overrun
0x01000703 Right edge of /
autoloading position
notch overrun
0x01000704 Left edge of /
autoloading position
notch overrun
0x01000705 Right edge of /
closed-tube position
notch overrun
0x01000706 Left edge of /
closed-tube position
notch overrun

0x01000801 Plunger pump A Check if the cable of the plunger pump on the right
initialization failed side is not connected properly. Check if there is
liquid in the sensor connector? (see silkscreen for
plunger pump type)
0x01000802 Plunger pump B Check if the cable of the plunger pump on the left
initialization failed side is not connected properly. Check if there is
liquid in the sensor connector? (see silkscreen for
plunger pump type)
0x01000803 Plunger pump A Same as above
movement failed
0x01000804 Plunger pump B Same as above
movement failed

0x01000901 Feeding valve No need to address it for now

switching failed
0x01000902 Rotation valve No need to address it for now
switching failed

0x01000A01 Degasser off /

0x01000A02 Degasser pressure /
not in control range

0x01000B01 EEPROM write EEPROM write error

error
0x01000B02 Action overtime Action overtime

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Performing Hemolysis Solution Floater is damaged

sequence floater status
overtime abnormal
0x01000C02 Eluent A floater Floater is damaged
status abnormal
0x01000C03 Fluidic initialization Some error has occurred during startup
not performed

0x01000D01 Sample Probe uses Sample Probe uses out of the upper limit of 60,000
out of limit times
0x01000D02 Filter uses out of Filter uses out of limit (the upper limit has not been
limit decided yet)
0x01000D03 Time for Probe 600 analyses have been performed, time for Probe
Cleanser Cleanser maintenance
maintenance
0x01000D04 Probe Cleanser The Probe Cleanser maintenance has not
maintenance failed completed. Some functions including analysis will be
forbidden
0x01000D05 Filter failed The error is reported when the column pressure
rises too fast. The old filter must be replaced, or the
column life span will drop

0x01000E01 Data length No need to address it

abnormal
0x01000E02 Optical signal No need to address it
abnormal

0x01005200 Loading motor busy Mix mechanism X direction motor action error
0x01005201 Manipulator pinch Mix Mechanism is working
motor busy
0x01005202 Manipulator pinch Mix mechanism X direction motor action failed1
motor failed to leave
home position
0x01005203 Manipulator pinch Mix mechanism X direction motor action failed2
motor failed to
return to home
position
0x01005204 Manipulator pinch Mix mechanism X direction motor action failed3
motor action
forbidden
0x01005205 Manipulator pinch Mix mechanism X direction motor is not initiated
motor initialization
failed

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0x01005206 Manipulator pinch Mix mechanism X direction motor action overtime

motor action
overtime
0x01005207 Manipulator pinch Mix mechanism X direction motor action failed1
motor failed to leave
end position
0x01005208 Manipulator pinch Mix mechanism X direction motor action failed2
motor failed to
return to end
position
0x01005209 Manipulator pinch Mix mechanism X direction sensor error
motor sensor
abnormal

0x01005400 Manipulator pinch Mix motor initialization error

motor initialization
forbidden
0x01005401 Manipulator Mix Mechanism is working
elevation motor
busy
0x01005402 Manipulator Mix mechanism Z direction motor action failed1
elevation motor
failed to leave home
position
0x01005403 Manipulator Mix mechanism Z direction motor action failed2
elevation motor
failed to return to
home position
0x01005404 Manipulator Mix motor Z direction motor current action forbidden
elevation motor
action forbidden
0x01005405 Manipulator Mix mechanism Z direction motor is not initiated
elevation motor
initialization failed
0x01005406 Manipulator Mix mechanism Z direction motor action overtime
elevation motor
action overtime
0x01005407 Manipulator Mix mechanism Z direction motor action failed1
elevation motor
failed to leave end
position
0x01005408 Manipulator Mix mechanism Z direction motor action failed2
elevation motor
failed to return to

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end position
0x01005409 Manipulator Mix mechanism Z direction sensor error
elevation motor
sensor abnormal

0x01005500 Manipulator Mix motor current action not allowed (no way to
elevation motor report this yet)
forbids mix motor
action
0x01005501 Mix motor busy Mix motor in action
0x01005502 Mix motor failed to Mix motor action error
leave home position
0x01005503 Mix motor failed to Mix motor action error
return to home
position
0x01005504 Mix motor action Mix motor action not allowed
forbidden
0x01005505 Mix motor Mix motor not initialized
initialization failed
0x01005506 Mix motor action Mix motor action overtime
overtime
0x01005507 Mix motor failed to Motor not supposed to be at the end position
leave end position
0x01005508 Mix motor failed to Motor supposed to be at the end position
return to end
position

0x01005601 Mix mechanism Mix Mechanism is working

busy
0x01005603 Mix mechanism Mix mechanism action overtime
action overtime
0x01005604 Mix time setting out Mix time setting out of limit
of limit
0x01005605 Mix mechanism Mix mechanism is not initiated
initialization failed
0x01005606 Mix mechanism Mix mechanism current action is forbidden
current action
forbidden

0x01005700 Feeding motor busy X-direction loading motor in action

0x01005701 Feeding motor X-direction loading motor action error 1
home position
sensor error

5-34
Software

0x01005702 Feeding position X-direction loading motor current action forbidden

tube rack error
0x01005703 Feeding motor end X-direction loading motor action error 2
position sensor
error
0x01005704 Feeding motor X-direction loading motor sensor error
sensor error
0x01005705 Feeding motor X-direction loading motor loading error
loading error
0x01005706 Feeding motor X-direction loading motor action overtime
action overtime
0x01005708 Feeding position Loading micro-switch error
micro switch error

0x01005801 Autoloader board Functional code error

functional code
error
0x01005802 Autoloader board Data length error
data length error
0x01005803 Autoloader board Command identifier error
command identifier
error
0x01005804 Autoloader board Check code error
check code error
0x01005805 Autoloader board End code error
end code error
0x01005806 Autoloader board Identification code error
identifier error

0x01005900 Loading motor Y-direction loading motor in action

receiving command
error
0x01005901 Loading motor Y-direction motor initialization forbidden
initialization
forbidden
0x01005902 Unloading tray full, Unloading action error
unloading forbidden
0x01005903 Loading motor Y-direction loading motor action error
home position
sensor error
0x01005904 Loading motor Unloading position sensor error
unloading position
sensor error
0x01005905 Loading position Y-direction right sensor error

5-35
Software

right sensor error

0x01005906 Loading position left Y-direction left sensor error
sensor error
0x01005907 Loading motor not Y-direction loading motor is not initialized
initialized
0x01005908 Loading motor Y-direction loading motor feeding error
feeding not at home
position
0x01005909 Loading motor Y-direction loading motor unloading error
unloading not at
home position
0x0100590A Loading motor Y-direction loading motor action overtime (no way to
action overtime report this yet)
0x0100590B Autoloader Command cannot be executed
receiving command
error (not in
sampling mode)
0x0100590C Autoloader Command cannot be executed
receiving command
error (not in stop
status)
0x0100590D Autoloader Y-direction left and right sensors cooperation error
horizontal loading
sensor match error
0x0100590E Unloading tray full Too many tubes are fed to the unloading tray during
when autoloader is autoloader initialization
initializing
0x0100590F Sensor blocked Tube detection sensor is blocked after initialization.
after autoloader
initialization
0x01005910 Autoloader Right sensor damaged, or unable to drag the tube
horizontal loading rack
sensor abnormal

0x01005a00 Autoloader board EEPROM data invalid

EEPROM chip error
0x01005a01 Failed to opening Opening sample compartment door failed
sample
compartment door
0x01005a02 Autoloader action All autoloader actions are currently forbidden when
forbidden when the autoloader is paused
autoloader is
paused
0x01005a03 Autoloader adjust The autoloader is not at adjusting status, so all

5-36
Software

forbidden when adjustment actions are forbidden

autoloader is not at
adjusting status
0x01005a04 Autoloader Adjustment parameter out of range
adjustment
parameter setting
out of range
0x01005a05 Scanner read error Read scanner error

5-37
Appendix
A Fluidic Diagrams

1 2 3 4 5 6 7 8
REV ECN DESCRIPTION DESIGN
MRSZ/R05N01.291.01（2.0） 2.0 EHK004 Modify BOM bug, add back pressure sensor Niu Zihua

1 2 1 2
A
VAC
6 3 6 3
C68 C69
（P5）T3（P4） T4-J3 5 4 5 4
SV09 T12 Load（POSB） Inject（POSA）
T11 GV10 C3
C70 C71 T17 C9 J6-T89-J11
（P6）T2（P3） T5-J4 T18 T98
T21 T24
SV08 C2 GV11 T13 C4
T10 C41
C72 C73 T16 C8 Steel tube 3 C42
C1 T19
（P7）T1（P2） T6-J5
T7 T20
SV07 T8 T14 C5 Detecor
T9 Preheating
T30 T15 C7 T109
GV12 steel tube 2 C40
T29 T28 T25 C82
C6 T26 T97
T22
B T31 T32 T33 GP1 T27
Isolation
C39
Thermostatic Chamber C84
C11 chamber T50
C13 T91
EL_A EL_B C67
cistern C15 cistern C10 T77 T80 C60 C59 PS1
T23 T107 OPEN
C81 C26 1 2
HWS Germproof 6 3 T85 Column Ta3
C85
cistern
试剂桶过试剂桶过试剂桶过 T34 filter C61 C64 C38 C37
滤器3 滤器2 滤器1 5 4
T90 T96
C16 C62 C63 T84
Eluate Eluate Hemolysis & Wash T79 T82
C14 T35 INJ_PUMPB T110
B A Solution Filter ROT-V
J2-T39 C25
T40 T43 J12-T95
C20 C21 C74 C75 C36
T49 Damper C27 C32 C35
1 2 Preheating
C17 SV17 T78
6 3 steel tube 1
C24 C33 C34 C28 C31
T36 Degasser Unit 5 4 Ta1
C29 C30
Pressure Transduser C66
INJ-V C65 C57
C18
INJ_PUMPA T48 T101
C C23 Ta2 T100
C56 T99
J1-T38 T41 T42 J7-T57-J13-T111 Sample Loop 4 uL OPEN
T37 C19 C22 C76 C77 T102
SV16 GV13
T47 J8-T59 C47

T46 T86 T88 名称: SV18

T56 SV19 Waste cistern
T45 C43 C90 C91 T92
T60(P1)
T94 C52
C58
SV20
C86 T76
C89
T93 J9
T82 C44 C46 C51
C45
T51 T52 T53
C83
Syringe 100uL
T108 T54
T61 T62

D SV03 SV02
T58 SV01
T81 SV04 Probe
T55
C87 T65 wipe T75
T44 T64
C88
Sample
probe

J10-T63 Probe wipe

混匀池 T66 filter
T67
C53 C54
C79
T74
T103 SV06 T70 SV05 T87
Syringe 2.5mL T69 C48 C55
T71
Mix bath filter
E C49 GP1
T104
T72 Waste
C80 C50 T73 container
SV15
T105 T68 Waste pump

保密密级：机密
CONFIDENTIAL
TITLE 3300 Fluidic diagram
DOC No. A1-115-027230-00
F 保密:此图及其全部知识产权(含著作权)归深圳迈瑞生物医疗电子股份有限公司所有。未经深圳迈瑞生物医疗电子股份有限公司预先书面许可,严禁出于任何目的,对此图的全部或部分内容(包括但不限于图中信息、数据、运算结果等)泄露、使用、拷贝或复制。 DESIGN Niu Zihua REV. 2.0
Classified documents, This set of drawing(s) and all it's intellectual property rights (including copyright) subsisting herein are property of Shenzhen Mindray Bio-medical Electronics Co.,Ltd. No disclosure,use,copies or reproductions
should be made of this drawing or any part(s) thereof for whatever purpose nor shall any information, data, calculations, or other contents contained in this drawing be disseminated without prior written permission of Shenzhen Mindray P.CODE 3300 SIZE A3
Bio-medical Electronics Co.,Ltd
Software & Rev:
SHEET 1 OF 12
Microsoft visio 2003

A-1
B List of Tubes and Connectors
Bill of Materials in 3300 Fluidic Diagram
No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Tube T1 082-002199-00 Spiral FEP tube, inner diameter 1.6mm Spiral tube FEP 1/16"IDX1/8"OD mm 1950
2 Tube T2 082-002199-00 Spiral FEP tube, inner diameter 1.6mm Spiral tube FEP 1/16"IDX1/8"OD mm 2080
3 Tube T3 082-002199-00 Spiral FEP tube, inner diameter 1.6mm Spiral tube FEP 1/16"IDX1/8"OD mm 2000
4 Tube T4 082-000036-00 PFA tube, inner diameter 1.6mm Tube.1/16＂X1/8＂,TSPF35-0125-031-50,PFA mm 200 46-200
5 Tube T5 082-000036-00 PFA tube, inner diameter 1.6mm Tube.1/16＂X1/8＂,TSPF35-0125-031-50,PFA mm 200 46-200
6 Tube T6 082-000036-00 PFA tube, inner diameter 1.6mm Tube.1/16＂X1/8＂,TSPF35-0125-031-50,PFA mm 200 46-200
7 Tube T7 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 50 6-50
8 Tube T8 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 50 6-50
9 Tube T9 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 110 30-110
10 Tube T10 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 130 30-130
11 Tube T11 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 170 30-170
12 Tube T12 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
13 Tube T13 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
14 Tube T14 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
15 Tube T15 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
16 Tube T16 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
17 Tube T17 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 20 30-20
18 Tube T18 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
19 Tube T19 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
20 Tube T20 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
21 Tube T21 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
22 Tube T22 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
23 Tube T23 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 180 30-180
24 Tube T24 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 690 30-690
25 Tube T25 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 13 30-13
26 Tube T26 082-000108-00 TPU tube, inner diameter 2mm Tube.M-87-D3,2mmX3.5mm,AV31X2103 mm 265 30-265

27 Tube T27 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 30 29-30
28 Tube T28 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 55 10-55
29 Tube T29 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 55 10-55
30 Tube T30 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 55 10-55
31 Tube T31 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 25 40-25
32 Tube T32 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 25 40-25
33 Tube T33 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 25 10-25
34 Tube T34 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 50 40-50
35 Tube T35 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 50 40-50
36 Tube T36 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 60 40-60
37 Tube T37 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 180 29-180
38 Tube T38 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 620 13-620
39 Tube T39 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 680 13-680
40 Tube T40 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 110 13-110
41 Tube T41 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 110 13-110
42 Tube T42 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 140 13-140
43 Tube T43 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 180 13-180
44 Tube T44 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 50 29-50
45 Tube T45 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 300 29-300
46 Tube T46 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 500 29-500
47 Tube T47 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 90 29-90
48 Tube T48 M90-000022--- Silicone tube,inner diameter 1.0 Tube.Silicone,0.04"X0.085",508-005 mm 40 47-40

B-1
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
49 Tube T49 M90-000022--- Silicone tube, inner diameter 1.0 Tube.Silicone,0.04"X0.085",508-005 mm 150 47-150
50 Tube T50 M90-000022--- Silicone tube, inner diameter 1.0 Tube.Silicone,0.04"X0.085",508-005 mm 100 47-100
51 Tube T51 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 15 29-15
52 Tube T52 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 15 29-15
53 Tube T53 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 15 29-15
54 Tube T54 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 20 29-20
55 Tube T55 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 150 29-150
56 Tube T56 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 20 2-20
57 Tube T57 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 600 13-600
58 Tube T58 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon 20 2-20
59 Tube T59 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 280 13-280
60 Tube T60 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 640 13-640 quantification
61 Tube T61 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 20 29-20
62 Tube T62 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 20 29-20
63 Tube T63 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 700 13-700
64 Tube T64 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 730 2-730
65 Tube T65 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 1420 1-1420
66 Tube T66 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 220 1-220
67 Tube T67 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 25 2-25
68 Tube T68 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 430 1-430
69 Tube T69 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 30 1-30
70 Tube T70 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 50 1-50
71 Tube T71 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 20 1-20
72 Tube T72 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 600 1-600
73 Tube T73 M90-000025--- Thick 3603 Flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 80 6-80
74 Tube T74 M90-000025--- Thick 3603 Flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 220 6-220
75 Tube T75 M90-100071--- Thick 50 Flexible tube Tube.3/32"X5/32",ND-100-65,Tygon mm 40 1-40
76 Tube T76 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 50 40-50
77 Tube T77 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 150 45-150
78 Tube T78 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 130 45-130
79 Tube T79 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 450 45-450
80 Tube T80 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 250 45-250
81 Tube T81 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon 20 2-20
82 Tube T82 082-001941-00 0.25PEEK tube Tub PEEK Nat 1/16x.010x5ft mm 160 44-160
83 Tube T83 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon 20 2-20
84 Tube T84 082-001941-00 0.25PEEK tube Tub PEEK Nat 1/16x.010x5ft mm 90 44-90
85 Tube T85 082-001941-00 0.25PEEK tube Tub PEEK Nat 1/16x.010x5ft mm 180 44-180
86 Tube T86 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon 20 2-20
87 Tube T87 M6G-020055--- TPU tube, inner diameter 3.2mm Tube.TPU,ID：1/8＂,OD：1/4＂,clear mm 1500 22-1500
88 Tube T88 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon 20 2-20
89 Tube T89 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 780 13-780
90 Tube T90 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 20 40-20
91 Tube T91 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 20 40-20
92 Tube T92 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 20 40-20
93 Tube T93 082-000432-00 Double layer tube, inner diameter 2mm Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 880 29-880
94 Tube T94 3001-10-07069 Thin 50 Flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 30 2-30
95 Tube T95 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 630 13-630
96 Tube T96 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 110 45-110

B-2
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
97 Tube T97 082-001941-00 0.25PEEK tube Tub PEEK Nat 1/16x.010x5ft mm 150 44-150
98 Tube T98 M6G-020008--- FEP tube, inner diameter 0.78 Tube.ChemfluorFEP,0.062"ODX0.031"ID mm 1230 13-1230
99 Tube T99 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 30 40-30
100 Tube T100 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 30 40-30
101 Tube T101 082-001276-00 Pharmed tube 3.2 Tube.1/8"X1/4",Pharmed mm 30 40-30
Double layer tube, inner diameter
102 Tube T102 082-000432-00 Tube.2X3.5mm PVDF(inner layer) TPU(outer layer) mm 300 29-300
2mm
103 Tube T103 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 20 6-20
104 Tube T104 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 20 6-20
105 Tube T105 3001-10-07069 Thin 50 flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 80 2-80
106 Tube
107 Tube T107 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 50 6-50
108 Tube T108 3001-10-07069 Thin 50 flexible tube Tube.1/16"X1/8",S-50-HLAAX02002,Tygon mm 20 29-20
109 Tube T109 082-001941-00 0.25PEEK tube Tub PEEK Nat 1/16x.010x5ft mm 250 44-250
110 Tube T110 082-001942-00 0.5PEEK tube Tub PEEK ora 1/16x.020x5ft mm 120 45-120
111 Tube T111 082-002207-00 0.75PEEK tube Tube.PEEK Gre,1/16X.030X5ft mm 170 48-170
112 Tube T112
113 Tube T113
114 Tube T114
115 Tube T115
116 Tube T116
117 Tube T117
118 Tube T118
119 Tube T119
120 Tube T120
121 Tube T121
122 Tube T122
123 Tube T123
124 Tube T124
125 Tube T125
126 Tube T126
127 Tube T127
128 Tube T128
129 Tube T129
130 Tube T130
131 Tube T131
132 Tube T132
133 Tube T133
134 Tube T134
135 Tube T135
136 Tube T136
137 Tube T137
138 Tube T138
139 Tube T139
140 Tube T140
141 Tube T141
142 Tube T142
143 Tube T143
144 Tube T144

B-3
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Adapter tube J1 0030-20-13339 Adapter tube Adapter tube(1.5mm-3mm)(mold MR13339) EA 1 28-20
2 Adapter tube J2 0030-20-13339 Adapter tube Adapter tube(1.5mm-3mm)(mold MR13339) EA 1 28-20
3 Adapter tube J3 M6G-020006--- Medium silicone tube, thick adapter tube
Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
4 Adapter tube J4 M6G-020006--- Medium silicone tube, thick adapter tube
Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
5 Adapter tube J5 M6G-020006--- Medium silicone tube, thick adapter tube
Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
6 Adapter tube J6 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
7 Adapter tube J7 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
8 Adapter tube J8 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
9 Adapter tube J9 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 25 37-25
10 Adapter tube J10 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
11 Adapter tube J11 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
12 Adapter tube J12 082-000710-00 Thin 3350 silicone tube Tube.silicone，0.031"X0.197",TYGON 3350 mm 20 37-20
13 Adapter tube J13 082-000710-00 Thin 3350 silicone tube
14 Adapter tube J14
15 Adapter tube J15
16 Adapter tube J16
17 Adapter tube J17
18 Adapter tube J18
19 Adapter tube J19
20 Adapter tube J20
21 Adapter tube J21
22 Adapter tube J22
23 Adapter tube J23
24 Adapter tube J24
25 Adapter tube J25
26 Adapter tube J26
27 Adapter tube J27
28 Adapter tube J28
29 Adapter tube J29
30 Adapter tube J30
31 Adapter tube J31
32 Adapter tube J32
33 Adapter tube J33
34 Adapter tube J34
35 Adapter tube J35
36 Adapter tube J36
37 Adapter tube J37
38 Adapter tube J38
39 Adapter tube J39
40 Adapter tube J40
41 Adapter tube J41
42 Adapter tube J42
43 Adapter tube J43
44 Adapter tube J44
45 Adapter tube J45
46 Adapter tube J46
47 Adapter tube J47
48 Adapter tube J48

B-4
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Annular tube P1 M90-100071--- Thick 50 flexible tube Tube.3/32"X5/32",S-50-HLAAX02004,Tygon mm 250 1-250
2 Annular tube P2 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 15 6-15
3 Annular tube P3 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 15 6-15
4 Annular tube P4 M90-000025--- Thick 3603 flexible tube Tube.1/8"X1/4",ACF02007(E-3603) mm 15 6-15
5 Annular tube P5 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
6 Annular tube P6 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
7 Annular tube P7 M6G-020006--- Medium silicone tube, thick adapter tube Tube.Silicone,1/16"X3/16",TYGON 3350 mm 20 10-20
8 Annular tube P8
9 Annular tube P9
10 Annular tube P10
11 Annular tube P11
12 Annular tube P12
13 Annular tube P13
14 Annular tube P14
15 Annular tube P15
16 Annular tube P16
17 Annular tube P17
18 Annular tube P18
19 Annular tube P19
20 Annular tube P20
21 Annular tube P21
22 Annular tube P22
23 Annular tube P23
24 Annular tube P24
25 Annular tube P25
26 Annular tube P26
27 Annular tube P27
28 Annular tube P28
29 Annular tube P29
30 Annular tube P30
31 Annular tube P31
32 Annular tube P32
33 Annular tube P33
34 Annular tube P34
35 Annular tube P35
36 Annular tube P36
37 Annular tube P37
38 Annular tube P38
39 Annular tube P39
40 Annular tube P40
41 Annular tube P41
42 Annular tube P42
43 Annular tube P43
44 Annular tube P44
45 Annular tube P45
46 Annular tube P46
47 Annular tube P47
48 Annular tube P48

B-5
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Connector C1 M90-100066--- L connector -big—medium-nylon L430/420-1 Connector.Elbow Reduction,1/8"&3/32"ID,White EA 1
2 Connector C2 M90-100027--- L connector -big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
3 Connector C3 082-001105-00 L connector—medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
4 Connector C4 082-001281-00 T connector—medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
5 Connector C5 082-001281-00 T connector—medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
6 Connector C6 082-001105-00 L connector—medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
7 Connector C7 082-001281-00 T connector—medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
8 Connector C8 082-001281-00 T connector—medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
9 Connector C9 082-001105-00 L connector—medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
10 Connector C10 082-001105-00 L connector—medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
11 Connector C11 043-000880-00 T connector-PEI 8.5mm 3-way connector EA 1
12 Connector
13 Connector C13 3001-10-07066 T connector-big-2 small Connector.Tee,200Barb,3/32"&1/8"ID,White Nylo EA 1
14 Connector C14 082-001960-00 Plug-big-PVDF-PIP230-J1A Plug for 1/8"ID tube, PVDF EA 1
15 Connector C15 082-001960-00 Plug-big-PVDF-PIP230-J1A Plug for 1/8"ID tube, PVDF EA 1
16 Connector C16 M90-100027--- T connector -big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
17 Connector C17 M90-100027--- I connector -big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
18 Connector C18 M90-100027--- I connector -big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
Connector 082-000525-00 Click-N-Seal connector Connector.CNS, 1/4-28 EA 1
19 C19
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
20 C20
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
21 C21
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
22 C22
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
23 Connector C23 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
24 Connector C24 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
25 Connector C25 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
26 Connector C26 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
27 Connector C27 082-001954-00 Rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
28 Connector C28 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
29 Connector C29 082-001954-00 Rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
30 Connector C30 082-001954-00 Rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
31 Connector C31 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
32 Connector C32 082-001954-00 Rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
33 Connector C33 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
34 Connector C34 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
35 Connector C35 082-001949-00 2-way PEEK connector Connector, PEEK, for 1/16"OD tube EA 1
36 Connector C36 082-001949-00 2-way PEEK connector Connector, PEEK, for 1/16"OD tube EA 1
37 Connector C37 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
38 Connector C38 082-001952-00 Integrated connector Connector, PEEK, matural color, for 1/16"OD tube EA 1
39 Connector C39 082-001949-00 2-way PEEK connector Connector, PEEK, for 1/16"OD tube EA 1
Connector 082-001947-00 Steel connector Connector, 316, for 1/16"OD tube EA 1
40 C40
Connector 082-001948-00 Steel connector coupling Coupling,316,for 1/16"OD tube EA 1
Connector 082-001947-00 Steel connector Connector, 316, for 1/16"OD tube EA 1
41 C41
Connector 082-001948-00 Steel connector coupling Coupling, 316, for 1/16"OD tube EA 1
42 Connector C42 082-001949-00 2-way PEEK connector Connector, PEEK, for 1/16"OD tube EA 1

B-6
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
43 Connector C43 043-000880-00 T Connector-PEI 8.5mm 3-way connector EA 1
44 Connector C44 043-000880-00 T Connector-PEI 8.5mm 3-way connector EA 1
45 Connector C45 043-000880-00 T Connector-PEI 8.5mm 3-way connector EA 1
46 Connector C46 043-000880-00 T Connector-PEI 8.5mm 3-way connector EA 1
47 Connector C47 082-001951-00 3-way PEEK connector 3-way connector,PEEK, for 1/16"OD tube EA 1
48 Connector C48 082-001281-00 T connector-medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
49 Connector C49 082-001281-00 T connector-medium-PVDF-T420-J1A Connector.T type 400barb 3/32" EA 1
50 Connector C50 3001-10-07066 T connector-big-2 small Connector.Tee,200Barb,3/32"&1/8"ID,White Nylo EA 1
51 Connector C51 M90-100027--- I connector-big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
52 Connector C52 082-001960-00 Plug-big-PVDF-PIP230-J1A Plug,for 1/8"ID tube,PVDF EA 1
53 Connector C53 M90-100009--- FTLB230-1 Connector.FemaleLuer,1/4-28UNF,1/8"ID EA 1
54 Connector C54 M90-100025--- MTL230-1 Connector.Male Luer,1/8"Barb,White Nylon EA 1
55 Connector C55 3102-20-69219 Waste tube connector Waste tube connector EA 1
56 Connector C56 M90-100027-00 I connector-big—medium-nylon-N420/420-1 Connector.StraightReduction,3/32"&1/16"ID EA 1
57 Connector C57 M90-100027--- I connector-big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
58 Connector C58 043-000880-00 T connector-PEI 8.5mm 3-way connector EA 1
59 Connector C59 082-001954-00 Long rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
60 Connector C60 082-001952-00 Integrated connector Connector,PEEK, natural color, for 1/19"OD tube EA 1
61 Connector C61 082-001954-00 Long rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
62 Connector C62 082-001952-00 Integrated connector Connector,PEEK, natural color, for 1/19"OD tube EA 1
63 Connector C63 082-001954-00 Long rotary integrated connector Connector, PEEK, for 1/16"OD tube EA 1
64 Connector C64 082-001952-00 Integrated connector Connector,PEEK, natural color, for 1/19"OD tube EA 1
65 Connector C65 M90-100027--- I connector-big—medium Connector.StraightReduction,1/8*&3/32*ID EA 1
66 Connector C66 M90-100027--- I connector-big—medium Connector.StraightReduction,1/8*&3/32*ID EA 1
67 Connector C67 082-001951-00 3-way PEEK connector 3-way connector,PEEK, for 1/16"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
68 C68
Connector 082-001956-00 1/4-28 coupling -1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
69 C69
Connector 082-001956-00 1/4-28 coupling-1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
70 C70
Connector 082-001956-00 1/4-28 coupling-1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
71 C71
Connector 082-001956-00 1/4-28 coupling-1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
72 C72
Connector 082-001956-00 1/4-28 coupling-1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector M6Q-030065--- 1/4-28 connector Connector.flangeless nut,black delrin,1/4-28UNF EA 1
73 C73
Connector 082-001956-00 1/4-28 coupling-1/8 Coupling, ETFE, for 1/8"OD tube EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
74 C74
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
75 C75
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
76 C76
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
77 C77
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
78 Connector
79 Connector C79 M90-100027--- I connector-big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
80 Connector C80 M90-100027--- I connector-big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1

B-7
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
81 Connector C81 M90-100027--- I connector-big—medium-nylon-N430/420-1 Connector.StraightReduction,1/8*&3/32*ID EA 1
82 Connector C82 M90-100027-00 I connector-big—medium-nylon-N420/410-1 Connector.StraightReduction,3/32"&1/16"ID EA 1
83 Connector C83 082-001105-00 L connector-medium-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
84 C84
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
Connector 082-000525-00 Click-N-Seal connector Connector. CNS, 1/4-28 EA 1
85 C85
Connector 082-000526-00 Click-N-Seal connector coupling Coupling.0.16"x0.19" EA 1
86 Connector C86 043-000892-00 L connector-medium-PVDF Straight tube connector EA 1
87 Connector C87 082-001105-00 L connector-medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
88 Connector C88 082-001105-00 L connector-medium-PVDF-L420-J1A Connector.Elbow,400Barb 3/32"ID natural PVDF EA 1
89 Connector C89 043-000892-00 I connector-medium-PVDF Straight tube connector EA 1
90 Connector C90 043-000892-00 I connector-medium-PVDF Straight tube connector EA 1
91 Connector C91 043-000892-00 I connector-medium-PVDF Straight tube connector EA 1
92 Connector
93 Connector
94 Connector
95 Connector
96 Connector
97 Connector
98 Connector
99 Connector
100 Connector
101 Connector
102 Connector
103 Connector
104 Connector
105 Connector
106 Connector
107 Connector
108 Connector
109 Connector
110 Connector
111 Connector
112 Connector
113 Connector
114 Connector
115 Connector
116 Connector
117 Connector
118 Connector
119 Connector
120 Connector
121 Connector
122 Connector
123 Connector
124 Connector
125 Connector
126 Connector
127 Connector

B-8
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Valve SV01 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC12V EA 1
2 Valve SV02 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC12V EA 1
3 Valve SV03 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC12V EA 1
4 Valve SV04 115-013056-00 3-way SMC valve Fluidic valve, 2-way, -75~250kPa DC12V EA 1
5 Valve SV05 115-010089-00 2-way Mindray valve 2-way miniature valve(short line, new terminal) EA 1
6 Valve SV06 115-010089-00 2-way Mindray valve 2-way miniature valve(short line, new terminal) EA 1
7 Valve SV07 082-000050-00 2-way Burkert valve Valve. 2-position, 2-way, 0－2bar 12VDC EA 1
8 Valve SV08 082-000050-00 2-way Burkert valve Valve. 2-position, 2-way, 0－2bar 13VDC EA 1
9 Valve SV09 082-000050-00 2-way Burkert valve Valve. 2-position, 2-way,0－2bar 14VDC EA 1
10 Valve GV10 115-013056-00 3-way SMC valve Fluidic valve, 3-way, -75~250kPa DC12V EA 1
11 Valve GV11 115-013056-00 3-way SMC valve Fluidic valve, 3-way, -75~250kPa DC12V EA 1
12 Valve GV12 115-013056-00 3-way SMC valve Fluidic valve, 3-way, -75~250kPa DC12V EA 1
13 Valve GV13 115-013056-00 3-way SMC valve Fluidic valve, 3-way, -75~250kPa DC13V EA 1
14 Valve SV14
15 Valve SV15 115-010089-00 2-way Mindray valve 2-way miniature valve(short line, new terminal) EA 1
16 Valve SV16 082-000050-00 2-way Burkert valve Valve. 2-position, 2-way, 0－2bar 13VDC EA 1
17 Valve SV17 082-000050-00 2-way Burkert valve Valve. 2-position, 2-way, 0－2bar 13VDC EA 1
18 Valve SV18 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC12V EA 1
19 Valve SV19 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC13V EA 1
20 Valve SV20 115-011404-00 2-way SMC valve Fluidic valve, 2-way, -75~250kPa DC14V EA 1
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48

B-9
List of Tubes and Connectors

Bill of Materials in 3300 Fluidic Diagram

No. Type Name in Diagram P/N Introduction Material Description Position Unit Quantity Use Remark
1 Cistern HWS cistern 115-027283-00 HWS cistern HWS cistern EA 1
2 Cistern EL_A cistern 115-027284-00 EL_A cistern EL_A cistern EA 1
3 Cistern EL_B cistern 115-027285-00 EL_B cistern EL_B cistern EA 1
4 Cistern Waste cistern 115-018614-00 Waste cistern Short cistern EA 1
5 Syringe Syringe 2.5mL 115-002531-00 2.5ml syringe 2.5ml syringe assembly EA 1
6 Syringe Syringe 100uL 115-011898-00 100ul syringe 100ul screwdrive syringe assembly EA 1
7 Mix bath Mix bath 041-017444-00 HbA1c mix bath HbA1c mix bath EA 1
8 Probe wipe Probe wipe 041-001013-00 Probe wipe Probe wipe EA 1
9 Sample probe Sample probe 115-014677-00 Sample probe Sample probe EA 1
10 Filter Probe wipe filter 115-011660-00 Probe wipe filter Filter assembly(injection molding) EA 1
11 Filter Mix bath filter BA40-10-62025 Mix bath filter Filter, HDPE4903/PP 1/8"45-90um EA 1
12 Filter Reagent container filter 1 082-001944-00 Reagent inlet filter Reagent inlet filter, A-302A PCTFE/SST 10um EA 1
13 Filter Reagent container filter 2 082-001944-00 Reagent inlet filter Reagent inlet filter, A-302A PCTFE/SST 10um EA 1
14 Filter Reagent container filter 3 082-001944-00 Reagent inlet filter Reagent inlet filter, A-302A PCTFE/SST 10um EA 1
15 Degasser Degasser 082-001943-002 2-channel degasser degasser, 2-channel, 285ul/channel EA 1
16 Plunger pump INJ_PUMPA 082-002128-00 Plunger pump Plunger pump, 5Mpa 4.5mL/min 0.169uL/step EA 1
17 Plunger pump INJ_PUMPB 082-002128-00 Plunger pump Plunger pump, 5Mpa 4.5mL/min 0.169uL/step EA 1
18 Pulse damper Damper 082-002127-00 Pulse damper Pulse damper, 2Mpa, 0.2mL EA 1
19
20 Feeding valve Injection Valve1 082-001931-00 Rotation valve Rotary feeding valve, 2-position, 6-way, 41MPa 24VDC EA 1
21 Rotation valve Injection Valve2 082-001931-00 Rotation valve Rotary feeding valve, 2-position, 6-way, 41MPa 24VDC EA 1
22 Column Column
23 Flow cell Detecor 115-027756-00 Flow cell assembly Flow cell assembly EA 1
3101-20-68325 Isolation chamber clamp Isolation chamber clamp EA 1
24 Isolation chamber Isolation chamber
3003-20-34949 Isolation chamber Isolation chamber EA 1
25 Steel tube Preheating steel tube 1 082-001966-00 300mm steel tube Steel tube,316L,Φ1.6*Φ0.25*300mm EA 1
26 Steel tube Preheating steel tube 1 082-001965-00 200mm steel tube Steel tube,316L,Φ1.6*Φ0.25*200mm EA 1
27 Steel tube Steel tube 3 082-001965-00 200mm steel tube Steel tube,316L,Φ1.6*Φ0.25*200mm EA 1
28 Waste pump Waste pump 3001-10-07252 Waste pump Rotary diaphragm pump EA 1
Include length of T60,
29 Sample loop Ta1 115-031856-00 Sample preparation channel Sample preparation channel mm 1290
theoretical central value
30 Sample loop Ta2 115-031857-00 Sample feeding tubing Sample feeding tubing mm 81 Theoretical central value
31 Sample loop Ta3 115-031858-00 Back pressure tubing Back pressure tubing mm 400 Theoretical central value
32 Sensor PS1 BA40-30-61616 Hydraulic sensor 3-way needle block detection assembly EA 1
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47

B-10
PN: 046-008090-00-1.0

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