Chamestry Lab PDF
Chamestry Lab PDF
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Mixture: A solution is a particular type of mixture. Mixtures in chemistry are combinations of
different substances where each substance retains its chemical properties. Generally, mixtures can
be separated by non-chemical means such as filtration, heating, or centrifugation.
Solution: A solution is a homogeneous mixture of two or more substances on the molecular level.
The constituent of the mixture present in a smaller amount is called solute and the one present in a
larger amount is called the solvent.
A solution containing a relatively low concentration of solute is called a dilute solution and a high
concentration is called a concentrated solution.
Molarity (M)
Example: A 1 M NaCl solution contains 1 mole of NaCl dissolved in enough water to make 1 liter
of solution. The molecular weight of a sodium chloride molecule (NaCl) is 58.5, so one gram-
molecular mass (=1 mole) is 58.5 g. If you dissolve 58.5 g of NaCl in a final volume of 1 liter, you
have made a 1M NaCl solution, a 1 molar solution.
Molality (m)
Normality (N)
Definition: Normality is defined as the number of equivalents of solute per liter of the solution.
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Example: 40 g of NaOH (eq. wt.= 40) be dissolved in one liter of solution, the normality of the
solution is one and the solution is called 1N. A solution containing 4 g of NaOH is 1/10 N or 0.1 N
❖ Molarity, S = 1000W / MV
To make molar NaCl solutions of other concentrations dilute the mass of salt to 1000ml of
solution as follows:
0.1M NaCl solution requires 0.1 x 58.5 g of NaCl = 5.85g
0.5M NaCl solution requires 0.5 x 58.5 g of NaCl = 29.2g
2M NaCl solution requires 2.0 x 58.5 g of NaCl = 117 g
Titrimetric Analysis
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Primary Standard
A primary standard is a highly pure, stable substance used to prepare standard solutions or to
calibrate analytical instruments. Its key characteristics include:
Examples: Sodium carbonate (Na₂CO₃), Oxalic acid (C₂H₂O₄ · 2H₂O), Potassium dichromate
(K₂Cr₂O₇)
• Lower purity: May contain impurities or may be more reactive with the atmosphere.
• Stability: Less stable compared to primary standards, so they need periodic standardization.
Examples: Sodium hydroxide (NaOH), Hydrochloric acid (HCl), Sulfuric acid (H₂SO₄)
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Experiment no : 1
Introduction:
Definition of molarity:
The number of moles of solute (the material dissolved) per liter of solution. Used to express
the concentration of a solution.
From the definition of molarity, it’s clear that, to prepare 1 M molar solution of NaOH, we
need to take 40gm of NaOH in 1L of H2O.
Purpose:
Calculation:
As we need to prepare .5M 100 ml NaOH solution, we need to do some calculation about,
how much NaOH required to prepare the desired .5M NaOH solution.
= 2 gm
Materials:
1. NaOH
2. Weighing Machine
3. Watch Glass
4. Glass road
5. Distilled water
6. 100 ml Volumetric flask
7. Funnel
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Procedure:
1. Rinse watch glass, funnel and volumetric flask with distilled water.
2. Weight the required amount of NaOH in the weighing machine.
3. Transfer weighted NaOH to the 100ml volumetric flask.
4. Fill-up the volumetric flask up to the mark with distilled water.
5. Dissolve NaOH properly by shaking the volumetric flask.
Experiment no : 2
Introduction:
Definition of molarity:
The number of moles of solute (the material dissolved) per liter of solution. Used to express
the concentration of a solution.
From the definition of molarity it’s clear that, to prepare 1 M molar solution of HCl, we need
to take 36.5gm of HCl in 1L of H2O.
Purpose:
Calculation:
As we need to prepare .5M 100 ml HCl solution, we need to do some calculation about, how
much HCl required to prepare the desired .5M HCl solution.
= 31.44 ml/mole
The given HCl strength is 35%.
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31.44∗100
So the required amount of HCl should be = 𝑚𝑙/𝑚𝑜𝑙𝑒
89.83
= 89.85 ml/mole
= 4.5 ml
Materials:
1. HCl
2. Measuring cylinder
3. Pipette
4. hand gloves
5. safety glasses (goggles)
6. Distilled water
7. 100 ml Volumetric flask
Procedure:
1. Rinse measuring cylinder, pipette and volumetric flask with distilled water.
2. Ware safety glass and hand gloves.
3. Measure the required amount of HCl using measuring cylinder.
4. Transfer measured HCl to the 100ml volumetric flask.
5. Fill-up the volumetric flask up to the mark with distilled water.
6. Mix properly by shaking the volumetric flask.
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Experiment no : 3
Introduction:
Neutralization reactions involve the reaction of an acid and a base to produce a salt (ionic
compound) and water.
This process involves a solution of known concentration (the titrant or standard solution)
delivered from a burette into the unknown solution (the analyte) until the substance being
analyzed is just consumed.
The moles of H+ = moles of OH- at this point (called the equivalence point).
Information about the analyte (i.e. concentration) can be calculated at the equivalence
point.
The end point in a titration is often signalled by the colour change of an indicator and
occurs just slightly past the equivalence point.
An indicator is a substance (weak acid) that has distinctively different colours in acidic and
basic media.
Materials:
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Procedure:
1. Rinse and fill a burette with standardized 0.5mol/L NaOH. Open the stopcock briefly to allow
any air bubbles to pass through. Record the initial volume of NaOH in the burette.
2. Add 10.00 ml of unknown HCl solution into an 250 ml conical flask. Add 2 drops of
phenolphthalein indicator.
3. Record the initial colors of both the NaOH and HCl solutions. Also, draw diagrams of
the flask and burette, showing the molecules present in each solution prior to the
titration.
4. Predict what the pH of the unknown HCl solution will be. Explain your prediction.
Then, using the digital pH meter, record the initial pH of the HCl solution in the
Erlenmeyer flask.
5. Gradually dispense some of the NaOH solution drop-by-drop from the burette into the
solution in the conical flask. Swirl the flask constantly as the drops are added. Note
any color changes observed, and do so constantly as NaOH is added to the HCl
solution.
6. As the NaOH is being added to the HCl, what is happening at the molecular level?
Draw diagrams of the flask and burette at this point, showing the molecules present in
each solution. Also, symbolically write the equation for the chemical reaction that is
taking place.
7. As the NaOH is being added, you will notice that the pH is increasing gradually. At
the molecular level, describe why there is a change in pH. Also describe the change in
pH using a chemical equation.
8. As the equivalence point is approached, a pinkish color will appear and dissipate more
slowly as the titration proceeds. Why does this occur? Now add the NaOH drop-by
drop until the endpoint of the titration is reached (this is the point at which a very light
pink color is obtained after 20 seconds of swirling the flask
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Calculation:
1.
2.
3.
Here,
We know that, S1V1 = S2V2 V1 = volume of NaOH
S1 = Strength of NaOH (.5M)
S2 =
𝑆1𝑉1
= S2= Strength of HCl ??
𝑉2 V2=Volume of HCl (10ml)
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Experiment no:4
Introduction:
Neutralization reactions involve the reaction of an acid and a base to produce a salt (ionic
compound) and water.
This process involves a solution of known concentration (the titrant or standard solution)
delivered from a burette into the unknown solution (the analyte) until the substance being
analyzed is just consumed.
The moles of H+ = moles of OH- at this point (called the equivalence point).
Information about the analyte (i.e. concentration) can be calculated at the equivalence
point.
The end point in a titration is often signaled by the color change of an indicator and occurs
just slightly past the equivalence point.
An indicator is a substance (weak acid) that has distinctively different colors in acidic and
basic media.
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Materials:
Procedure:
0.63
(ii)Take 10 ml of supplied NaOH solution in a conical flask by means of a pipette & dilute it
to about 50ml.Add one drop of phenolphthalein indicator to the solution.
(iii) Then add standard oxalic acid solution drop by drop from a burette. Shake the flask
frequently while adding the acid solution.
(iv)Stop the addition of oxalic acid solution as soon as the pink color of the solution just
disappears. Note the burette reading.
(v)The burette reading should be taken carefully at the lower meniscus of the liquid.
Difference of the initial &final burette reading gives the volume of the acid added.
(vi)The process should be repeated at least thrice. These should agree within 0 .1ml. Calculate
the normality of the supplied NaOH solution using the following relation.
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Calculation:
1.
2.
3.
Here,
We know that, S1V1 = S2V2 V1 = volume of oxalic acid
S1 = Strength of oxalic acid
S2 =
𝑆1𝑉1
= S2= Strength of NaOH??
𝑉2 V2=Volume of NaOH(10ml)
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Experiment no:5
Introduction:
Neutralization reactions involve the reaction of an acid and a base to produce a salt (ionic
compound) and water.
Example: Neutralization reaction between Na2CO3 and HCl acid takes place into two
steps-
This process involves a solution of known concentration (the titrant or standard solution)
delivered from a burette into the unknown solution (the analyte) until the substance being
analyzed is just consumed.
The moles of H+ = moles of OH- at this point (called the equivalence point).
Information about the analyte (i.e. concentration) can be calculated at the equivalence
point.
The end point in a titration is often signaled by the color change of an indicator and occurs
just slightly past the equivalence point.
An indicator is a substance (weak acid) that has distinctively different colors in acidic and
basic media.
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Materials:
1. Standardized Na2CO3solution
2. Unknown HCl solution**
3. Phenolphthalein indicator solution
4. 2-50 mL Burettes
5. Universal Stand
6. Titration Clamp
7. 250 mL conical flask
8. Distilled water
Procedure:
Transfer .5 3g of anhydrous Na2CO3 in a100 ml volumetric flask then dissolve it with distilled
water up to the mark. Normality of the prepared acid solution will be calculated as
0.53
(i) Rinse and fill a burette with standardized 0.1 mol/HCl solution. Open the stopcock briefly
to allow any air bubbles to pass through. Record the initial volume of HCl in the burette.
(ii)Take 10 ml of supplied Na2CO3 solution in a conical flask by means of a pipette & dilute it
to about 50ml.Add 2-3 drops of phenolphthalein indicator &titrate against HCl contained in a
burette.
(iii) Now note the burette reading when just one drop of HCl discharges the pink color of the
solution. This the first end point.
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(iv)Then add 3-4drops of methyl orange inside the same conical flask &continue titration
against the same HCl acid. The end point reached when the color of the solution just changes
to faint pink. Note the burette reading.
(v)This the second end point. The difference of the burette reading from initial to Second end
point will be the volume of the acid required in the titration.
(vi )Repeat the whole experiment twice & these should agree within 0.1ml initial to second
end point .Calculate the strength of dil.HCl acid & then find out the normality of commercial
conc. HCl acid.
Calculation:
1.
2.
3.
Here,
We know that, S1V1 = S2V2 V1 = volume of HCl
S1 = Strength of HCl
S2 =
𝑆1𝑉1
= S2= Strength of Na2CO3??
𝑉2 V2=Volume of Na2CO3 (10ml)
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Experiment 2 Volumee of Burette reading Difference Average
Na2CO3 ml Initial volume Final volume volumee (final volume ml (V3)
(V2) ml ml –
initial
) ml
4.
5.
6.
Here,
We know that, S3V3 = S2V2 V3 = volume of HCl
S3 = Strength of HCl??
S3= S2V2 = S2= Strength of Na2CO3
V2=Volume of Na2CO3
V3
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Practice multiple choice answers
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9) How many decimeters are there in 15 centimeters?
A) 150 dm
B) 1.5 dm
C) 0.15 dm
D) None of these
10) How many kilograms are there in 4.21 pounds? There are 2.2 pounds in 1 kilogram.
A) 9.26 kg
B) 1.91 kg
C) 0.523 kg
D) None of these
11) A homogenous material is defined as being:
A) An element
B) Any material with uniform composition
C) Synonymous with “solution”
D) More than one of these
12) An example of a chemical property is:
A) Density
B) Mass
C) Acidity
D) Solubility
13) “Exothermic” processes:
A) Absorb energy
B) Give off energy
C) Have no energy change
D) It is impossible to predict the energy change of an exothermic process.
14) Intrinsic properties are properties that:
A) Don’t depend on the amount of material present.
B) Depend on the amount of material present.
C) Cannot be measured without performing a chemical reaction.
D) None of the above is correct.
15) What is the density of an object with a volume of 15 mL and a mass of 42 grams?
A) 0.352 g/ml
B) 2.80 g/ml
C) 630 g/ml
D) None of the above is correct.
16) How many electrons does Iron have?
A) 26
B) 17
C) 11
D) It depends on the isotope of iron
17) How many electrons does Sodium have?
A) 26
B) 17
C) 11
D) It depends on the isotope of sodium
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18) How many electrons does Chlorine have?
A) 26
B) 17
C) 11
D) It depends on the isotope of Chlorine
19) Orbitals hold:
A) A maximum of one electron each
B) A maximum of two electrons each
C) A number of electrons that depends on the energy level.
D) A number of electrons that depends on the type of orbital.
20) Which of the following typically has a low melting point?
A) Metals
B) Nonmetals
C) Metalloids
D) Transition metals
21) Cations have:
A) Positive charge
B) Negative charge
C) No charge
D) It is impossible to predict the charge on a cation.
22) Anions have:
A) Positive charge
B) Negative charge
C) No charge
D) It is impossible to predict the charge on a anion.
23) Which one is Cation
A) Na+
B) Cl-
C) NaOH
D) HCl
24) Which one is Anion
A) Na+
B) Cl-
C) NaOH
D) HCl
25) Which one is Acid
A) Na+
B) Cl-
C) NaOH
D) HCl
26) Which one is Base
A) Na+
B) Cl-
C) NaOH
D) HCl
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27) How many grams are there in 2.1 moles of Sodium? 1mole of Na ≡ 23 gm.
A) 48.3 grams
B) 0.0913 grams
C) 11.0 grams
D) None of these is correct
28) The symbol (s) after a chemical compound lets you know that it is:
A) Soluble in water
B) Insoluble in water
C) A solid
D) More than one of the above
29) When water and carbon dioxide are formed during an exothermic reaction, it’s probably a:
A) Synthesis reaction
B) Combustion reaction
C) Single displacement reaction
D) Double displacement reaction
30) How many grams of carbon dioxide will be formed when 100 grams of CH4 is burned in oxygen?
A) 122 grams
B) 275 grams
C) 488 grams
D) None of these
31) If 100 grams of carbon dioxide are reacted with 50 grams of oxygen, what will the limiting
reagent be?
A) Carbon disulfide
B) Carbon dioxide
C) Oxygen
D) Sulfur
32) If I dilute 5 mL of 0.15 M NaCl to a final volume of 5 L, what’s the final concentration of NaCl?
A) 0.00015 M
B) 0.0015 M
C) 15000 M
D) None of these
33) Which of the following is not an acid?
A) HNO3
B) CH3COOH
C) H2SO4
D) All of these are acids
34) If a solution conducts electricity, it is probably:
A) An acid
B) A base
C) Neutral
D) It is impossible to guess.
35) If a compound has a pH of 6.5, it has a pOH of:
A) 6.5
B) 7.5
C) 3.16 x 10-7
D) 3.16 x 10-8
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36) What is the difference between the endpoint and equivalence point in a titration?
A) The endpoint is when the pH is exactly 7
B) The equivalence point is when the pH is exactly 7
C) The endpoint and the equivalence point are the same thing.
D) None of these answers is correct.
37) What is the purpose of an indicator in the solution with the unknown concentration?
A) It tells when there is enough acid in the solution
B) It tells when the equivalence point is obtained
C) It tells when there is enough base in the solution
D) None of these
38) If it takes 5 mL of 1.4 M NaOH to neutralize 150 mL of HCl with an unknown concentration,
what was the original concentration of the acid?
A) 0.47 M
B) 0.047 M
C) 0.014 M
D) None of these
39) True or false : Bases are sometimes called alkalies
A) True
B) False
40) Neutral solution have pH of
A)0
B)7
C) 14
D) none of these
41) Which is true?
A) pH of less than 7 is basic; pH of more than 7 is acidic
B) pH of less than 7 is acidic; pH of more than 7 is basic
42) Blood have a pH of
A) 6.4
B) 7
C) 7.4
D) 9.8
43) Molecular weight of NaOH
A) 40
B) 38
C) 48
D) 44
44) Which one is strong Acid
A) NaOH
B) H2SO4
C) Oxalic acid
D) HCl
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45) Which one is strong base
A) NaOH
B) H2SO4
C) NH3
D) HCl
46) What is the pH range?
A) 1 – 14
B) 0 – 14
C) 2 – 14
D) none of these
47) Which is primary standard substance
A) HCl
B) NaOH
C) NaCl
D) H2O
48) Which is Secondary standard substance
A) HCl
B) NaOH
C) NaCl
D) H2O
49) Which reaction is correct
A) HCl + 2NaOH = NaCl + H2O
B) 2HCl + NaOH = NaCl + H2O
C) HCl + NaOH = NaCl + H2O
D) HCl + 2NaOH = 2NaCl + H2O
50) Molecular weight of HCl is
A) 36.5
B) 35.5
C) 47
D) 35
Solution:
1.A 2.A 3.C 4.B 5.C 6.B 7.B 8.C 9.B 10.B
11.B 12.C 13.B 14.A 15.B 16.A 17.C 18.B 19.B 20.B
21.A 22.B 23.A 24.B 25.D 26.C 27.A 28.C 29.B 30.B
31.C 32.A 33.D 34.D 35.B 36.B 37.B 38.B 39.A 40.B
41.B 42.C 43.A 44.B 45.A 46.B 47.A 48.B 49.C 50.A
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