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2023-04 - Cleaning Verification Fill Good

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58 views4 pages

2023-04 - Cleaning Verification Fill Good

Uploaded by

ndat26834
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Suggestions for cleaning effectiveness verification

Scope/goal: Performing, before starting the production, a hygienic verification of Galdi


machine after a Cip/Sip cycle and manual cleaning, using ATP swabs to verify the
cleaning effectiveness of the most critical machine surfaces.

Material needed: Kikkoman PD-30 instrument, LuciPac Pen surface swabs.

Introduction:
ATP is an energy molecule found in all organic matter (living or once-living), including plants, animals and
microbial cells. It fuels metabolic processes such as cellular reproduction, muscle contraction, plant
photosynthesis, and respiration in fungi and fermentation in yeast. The detection of ATP on a surface
indicates the presence of biological matter that may not otherwise be visible. ATP Testing Device contains
a natural enzyme, called luciferase, that produces a simple bioluminescence (light-producing) reaction
when it comes into contact with ATP.
Measuring the amount of bioluminescence from an ATP reaction provides a good indication of surface
cleanliness or water quality because the quantity of light generated by the reaction is directly proportional
to the amount of ATP present in the sample. Results are then expressed numerically on the luminometer’s
screen as Relative Light Units (RLU). The relationship between the amount of ATP collected in a sample
and the RLU result displayed on the luminometer is linear, which makes understanding the technology
very easy.

To verify the machine hygienic condition before starting the production, Galdi suggests to verify the ATP
presence in the most critical machine surfaces, using LuciPac Pen surface swabs and Kikkoman PD-30
instrument.

Test preparation:
Before starting the test, the machine has to be properly cleaned performing both CIP and SIP cycles and
manual cleaning. In particular the following steps should be done before starting the test:
• a complete CIP cycle including alkali and acid phases (see machine manual for details);
• a SIP sanification cycle (see machine manual for details);
• a manual cleaning of all machine parts that contact indirectly the food product (see machine
manual for details).

FILL GOOD S.R.L. a socio unico


Via Cal Trevisana 26/B 31044 Montebelluna (TV) Pag. 1 | 4
P.IVA: 04935240269 – www.fill-good.it
Test procedure:
Before collecting a sample for testing, the surface should be visibly clean. If any soiling or residue is
apparent, re-clean the work area before testing. By doing it, you can remove any residue detected by the
device with proper cleaning; the sanification will be more effective when surfaces are free of all residue.
1. Wear clean, powder-free gloves.
2. Remove swabs from cold storage and leave for 10 minutes at room temperature before use.
3. Remove the swab from the outer tube. Do not touch the swab tip or shaft with fingers or anything
else, as this will contaminate the test.

4. Moisten the tip of the swab with tap water.


5. Pressing firmly down on the swab tip, collect a sample from a 5 x 5 cm area making a criss-cross
pattern. Use a side-to-side and up-and-down motion while rotating the swab tip, these
movements help breaking through any biofilm. Where 5 x 5 cm square sampling is not feasible,
swab as much of the surface as possible.

6. Return the sampling stick to the main body (casing) and push it all the way into the main body. In
order to open the reaction chamber, push firmly onto LuciPac Pen casing.

7. Identify the sample in a precise way, indicating the sample location, the date and time, the
operator and as much information as possible on the swab tube.

FILL GOOD S.R.L. a socio unico


Via Cal Trevisana 26/B 31044 Montebelluna (TV) Pag. 2 | 4
P.IVA: 04935240269 – www.fill-good.it
8. Shake the LuciPac Pen repeatedly so that all liquid in the capsule falls into the reaction tube. For
optimal results, the test should be run on the luminometer as soon as possible, and within 60
seconds of activation.

9. Open the lid on the Luminometer and insert the activated device into the reading chamber. Close
the lid, making sure to keep the machine upright and press ‘ENTER’ to initiate reading. The test
result will appear on the screen in 10 seconds.

10. Register the results in the form (see pag.5) and verify the compliance of the obtained values.

Points to be checked during the test:


It is important to identify areas that are at risk for contamination from product residue or microbe growth;
these areas should be designated as “control points” in the hygiene monitoring plan. A control point
should provide reliable feedback on the cleanliness of a particular piece of equipment or area during
testing. It is important that ATP testing is routinely performed on all control points, and that results are
stored and periodically evaluated.
Setting correct Pass, Caution and Fail levels is a fundamental part of running a successful ATP hygiene
monitoring program.
Galdi provides a list of suggested points to be checked.
All points analysed with surface swabs should result compliant to the cleanliness Galdi criteria, that is they
should have ATP values comprised in the established acceptance range.
Therefore, we can assume that the cleaning and sanification procedures applied on the machine are
successful, and the Galdi filling machine is ready to start production.

Results interpretation and corrective actions:


Implementing a corrective action plan is an essential part of the ATP Detection Program. Corrective action
procedures provide clear instructions for what steps should be taken following Pass/Caution/Fail results.

- Pass result: the control point surface has been adequately cleaned. Proceed with production.

FILL GOOD S.R.L. a socio unico


Via Cal Trevisana 26/B 31044 Montebelluna (TV) Pag. 3 | 4
P.IVA: 04935240269 – www.fill-good.it
- Caution result: the control point surface may not have been adequately cleaned. You can proceed with
production but it is suggested to monitor this control point in future tests.

- Fail result: the control point is dirty or contaminated, it must be re-cleaned and re-tested until a Pass or
Caution result is obtained. Monitor this control point in future tests. it may be decided that control points
which produce Fail results should be re-tested until 3 consecutive days of Pass results are achieved.

Analysis of ATP test result trends is necessary for evaluating the ongoing improvements of the cleaning
procedures: it is possible to monitor and assess ATP test results, perform trend analysis, identify trouble
zones, correct improper cleaning procedures, and eliminate risk. If trends show high numbers of Caution
and Fail results in ATP testing, procedures should be reviewed to improve protocols. If low numbers of
Caution and Fail results are obtained, Pass/Fail thresholds should be reviewed and lowered to maintain
high standards and ensure continuous improvement.

Pass, Caution and Fail thresholds should also be re-evaluated whenever procedural changes are made. As
sanitation procedures become more efficient and effective, thresholds should be adjusted to ensure your
facility is operating to its maximum potential.

FILL GOOD S.R.L. a socio unico


Via Cal Trevisana 26/B 31044 Montebelluna (TV) Pag. 4 | 4
P.IVA: 04935240269 – www.fill-good.it

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