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Glucose Pap

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50 views2 pages

Glucose Pap

Uploaded by

James
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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GLUCOSE PAP

Mono liquid

SAMPLES
Cat.No Package Size Serum free of hemolysis. Plasma collected on
GL450S98 4 x 50 mL + Standard (3 mL) fluorure or heparin + inhibitors of glycolysis.
GL4100S27 4 x 100 mL+ Standard (3 mL) Cerebral spinal fluid.
GL2125S136 2 x 125 mL + Standard (3 mL)
GL2250S73 2 x 250 mL+ Standard (3 mL) REFERENCE VALUES
GL2500S74 2 x 500 mL+ Standard (3 mL) [mg/dl] [mmol/l]
GL200H126 Hit I 4 x 50 ml Newborn
Umbilical cord blood 63 - 158 3,5 – 8,8
GL400H226 Hit II 4 x 100 ml 1h 36 - 99 2,0 – 5,5
2h 36 – 89 2,2 – 4,9
5 – 14 h 34 – 77 1,9 – 4,3
10 – 28 h 46 – 81 2,6 – 4,5
METHOD 44 – 52 h 48 – 79 2,7 – 4,4
Enzymatic – colorimetric. Trinder. End point. Children
1 – 6 Jahre 74 – 127 4,1 – 7,0
PRINCIPLE 7 – 19 Jahre 70 – 106 3,9 – 5,9
Enzymatic colorimetric determination of glucose Adults
Venous Plasma 70 – 115 3,9 – 6,4
according to the following reactions:
Note: It is recommended for each laboratory to establish and maintain its
Glucose oxidase own reference values. The given data here are only an indication.
Glucose + O2 Gluconic acid + H2O2

Peroxidase
2H2O2 + Phenol + 4-Aminoantipyrine Red quinone + 4H20 PROCEDURE
This reagent can be used manually (see method
below) and on most analyzers. Applications are
REAGENTS COMPOSITION available on request

Reagent: Wavelength : 500 nm (492-550 nm)


Phosphate Buffer (pH 7.5) 0,1 mol/l Temperature : 37° C
Phenol 0,75 mmol/l Cuvette : 1 cm light path
4-Aminoantipyrin 0,25 mmol/l
Glucoseoxidase (GOD) ≥ 15 kU/l BLANK STANDARD SAMPLE
Peroxidase(POD) ≥ 1,5U/l Reagent R1 1 mL 1 mL 1 mL
Standard: 100 mg/dl (5,55 mmol/l) Distilled water 10 µL - -
Standard -
Sample - 10 µL
PRECAUTIONS - - 10 µL
- For in vitro diagnostic use only. Mix and measure the absorbance (A) after a
- The reagent contains less than 0,95 g/l sodium 5 minute incubation, up to 60 min .
azide : Avoid contact with skin and mucous
membranes!
- Use clean or single use glass material only to CALCULATION
avoid contaminations. A Sample mg/dL c = 100
A Standard x c g/L c = 1
STABILITY OF REAGENTS
mmol/L c = 5.56
When stored at 2-8° C and protected from light, the
reagent is stable until the expiry date printed on the
label. c = standard concentration

PREPARATION AND STABILITY


OF WORKING REAGENT CALIBRATORS & CONTROLS
The reagent is ready for use. For the calibration of automated analyzers Inmesco
Multicalibrator Introcal is recommended, for quality
control use Inmesco normal and abnormal control,
Intronorm I and Intronorm II.

L-S 04/2009 page 1/2


PERFORMANCE DATA BIBLIOGRAPHY
st
-Linearity 1. Thomas L. Clinical Laboratory Diagnostics. 1 ed.
The method is linear up to 400 mg / dL Frankfurt: TH-Books Verlagsgesellschaft; 1998. p.
131-7.
- Detection limit 2. Sacks DB. Carbohydrates. In: Burtis CA, Ashwood
rd
ER, editors. Tietz Textbook of Clinical Chemistry. 3
The detection limit is equal 0.5 mg/dl ed. Philadelphia: W.B Saunders Company; 1999.
p. 750-808.
- Precision 3. Barham D, Trinder P. An improved color reagent for
Within-run reproducibility on serum the determination of blood glucose by the oxidase
N = 11 system. Analyst 1972;97:142-5.
Mean SD CV
mg/dl mg/dl %
Control 1 104,1 1,38 1,32%
Control 2 242,4 2,16 0,89% SYMBOLS USED
Patient 108,0 1,48 1,37%

For in vitro diagnostic medical use

Between-run reproducibility on serum


N = 11 Batch Code
Mean SD CV
mg/dl mg/dl %
Control 1 101,9 1,81 1,78% Use by
Control 2 240,4 1,63 0,68%
Patient 105,5 1,44 1,36%
Temperature limitation
- Correlation
A comparative study has been performed
between the Inmesco method and another
commercial reagent on 33 human serum
samples.The parameters of linear regression
are as follows :

y = 1.002 x + 0.305 mg/dl r = 1.000

INTERFERENCES
INTERFERENCES

- Ascorbic acid: no interference until 20 mg/dL

- Bilirubin: no interference until 40 mg/dL

- Hemoglobin: no interference until 500 mg/dL

- Triglycerides: no interference up to
2000 mg/dL

L-S 04/2009 page 2/2

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