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    Answers To Questions Related To 1st Year Biochemistry Course

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    Answers To Questions Related To 1st Year Biochemistry Course

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    1 views7 pages

    Answers To Questions Related To 1st Year Biochemistry Course

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    baggie.baggie.bags

    Copyright:

    © All Rights Reserved
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    Download as pdf or txt
    of 7

    Question 1

    Use the Michaelis Menten equation.


    The following values have been found for an enzym:
    -kcat = 30 s-1
    -Km = 0.005 M
    -Vmax = 10 mmol ml-1 min-1
    a. At what substrate concentration will the enzyme operate at 25% of its maximal rate
    b. What will the rate by at [S]= 0.0025 M (=0.5*Km); [S]= 0.01 M (=2*Km); [S]= 0.02
    M (=4*Km)?
    Question 2
    Two reactions take place in cells that allow the transfer of amino groups from glutamate to a-
    keto acids:

    Glutamate + pyruvate ⇆ a-ketoglutarate + alanine DG°’ = -1.0 kJ/mol


    Glutamate + oxaloacetate ⇆ a-ketoglutarate + aspartate DG°’ = -4.8 kJ/mol

    In cells the molar concentrations of the reactants are:

    [Pyruvate] = 10-2 M [Aspartate] = 10-2 M


    [Alanine] = 10-4 M [Oxaloacetate] = 10-5 M

    Does net synthesis of alanine and oxaloacetate from pyruvate and aspartate occur in these
    conditions?

    Gas constant, R = 8.315 x 10-3 kJ mol-1 K-1; temperatuur, T = 310 K


    Question 3
    The amino acid sequence of a small protein (a peptide) is:
    EHWSGLRPG

    What is the net charge of the peptide dissolved in water at pH 6 and pH 8? Use the
    information on amino acids at the end of this document.

    The amino group at the N-terminus (pKa 9, so one positive charge at pH 6 and pH8)
    Side chain carboxylate of glutamate (pKa 4, so one negative charge at pH 6 and pH8)
    Side chain imidazol of histidine (pKa 6, so uncharged at pH8; at pH 6 there are two
    populations: 50% of the molecules is uncharged and 50% has one positive charge at
    the imidazole group)
    The side chain guanidine um group of arginine (pKa 12.5, so one positive charge at
    pH 6 and pH8)
    The C-terminal carboxylate (pKa 3, so one negative charge at pH 6 and pH8)
    Question 4
    (G)
    (A)

    (F)

    (N) (Q) (V) (Y, pKa=11) (W)

    (S)

    (T) (C, pKa=8)

    (L) (I) (M)

    (D, pKa=4) (E, pKa=4)) (P)


    (K, pKa=11) (R, pKa=12) (H, pKa=6)
    (pKa N-terminal amino group = 9; pKa C-terminal carboxylate =3)

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