Rheodyne Manual Sample Injector Valves

Shaft End
Rotor Seal Rotor Seal Groove

Stator Face
Stator Face Seal
Stator

MBB Passage
Pump Column
MBB Passage

Position A
Rotor Seal Grooves Rotor Seal Grooves

MBB Passage MBB Passage

Position B Position C

Flow paths showing the patented Rheodyne MBB design

Waste Waste
6 6
1 Waste 1 Waste
5 5
Needle Port
2 2
4 Needle Port 4
7725 and 9725 Sample Injectors Pump 3 Pump 3
HPLC Accessories

• Continuous flow during switching Column Column

• A front-end pressure screw – for easy seal adjustment PositionAA(LOAD)

Position (LOAD) PositionBB(INJECT)
Position (INJECT)
• Wide port angles – for improved access to fittings Flow paths of the load and inject positions of the anayltical, micro-scale
• Internal position sensing switch – (“i” version) and preparative sample injectors, 7725, 9725, 8125 and 3725.
• Capability of reproducible 2µL sample injection with a
2µL internal sample loop
7725 and 9725 Sample Injectors continued
These HPLC sample injectors can inject from 1µL to 5mL with high
The needle port and syringe are never exposed to high pressure.
accuracy and precision. Easy to use and versatile, they allow both
They are automatically isolated when you turn to the INJECT
partial-filling and complete-filling methods of loading the loop.
position.
Rugged design and easy maintenance ensure long-term reliability.
In the Model 7725i and 9725i, turning to INJECT closes a built-in
Easy to use position sensing switch (reed switch), which remains closed until you
Insert a syringe into the needle port, dispense the desired amount of return to LOAD. When the wires are attached to a chromatograph,
sample, then turn the handle to the INJECT position. After removing the switch provides the system with a reproducible start signal.
the syringe and returning to LOAD, you are ready for the next
The 7725 and 7725i are set for 5000 psi (345 bar, 34 MPa). They are
injection. Flushing after every injection to prevent cross
adjustable to 7000 psi (483 bar, 48 MPa) by using the pressure-
contamination is not necessary; a patented direct-connection port
adjusting screw behind the handle.
design connects the tip of the needle directly to the end of the sample
loop so there is no connecting passage to trap sample. Valve accessories can be found on pages 180-183.

Eleven interchangeable loops from 2µL to 5mL are available. A 20µL Sample Injector Ordering Information
loop is supplied. These loops are not interchangeable with those used Cat. No. Description
on the Model 7125 injector. 7725 Sample injector, SS
7725I Sample injector, SS, position sensing switch
Reliable design, simple maintenance
9725 Sample injector, PEEK
High-pressure switching takes place between a polymeric rotor seal
9725I Sample injector, PEEK, pos. sensing switch
and ceramic stator face. The ceramic surface allows these injectors to
make 30,000 injections (in a clean system) before the rotor seal
needs replacing, a task requiring only twenty minutes. You can
tighten the seal from the front of the injector by turning the easily 7125 Syringe Loading Sample Injector
accessible pressure screw.
The Model 7125 can use both partial and
“Make-Before-Break”
complete loop filling. Valve accessories
The flow is not interrupted when the injector is switched between
can be found on pages 180-183.
LOAD and INJECT, a benefit when using flow-sensitive detectors,
Syringes used with the 7125 must have
fragile columns, or pumps that are disturbed by flow or pressure
the 22 gauge blunt tip needle.
transients. A passage in the stator face makes new connections before
old ones break. This patented make-before-break or “MBB” design Model 7725 replaces model 7125.
is an improvement over injectors which use a bypass; it is easy to Cat. No. Description
troubleshoot and does not dilute sample. 7125 Sample injector, includes 20µL sample loop

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 Rheodyne Manual Micro-Scale Sample Injector Valves
Model 7410
Model 8125 Micro-Scale Injector Low-Dispersion Micro-Scale Injector
The 8125 is designed for micro-scale The Model 7410 is a single mode
analysis which can partial fill 0.1µL injector (uses complete-filling
with zero sample loss, high accuracy, method) with an interchangeable
and low dispersion, yet can inject internal sample loop ideal for micro-
larger volumes by both partial and scale analysis. Loop discs of 0.5, 1,
complete filling. 2, and 5µL volume are available; lµL
is standard.
The 8125 has special small-bore 5,
10, and 20µL loops. To save loading The loop is filled by flowing sample
time in to the 8125's small flow into either port 1 or 4 while in the
passages, the largest loop LOAD position. The Model 7012
recommended for the complete- Loop Filler Port or 9013 Needle Port
filling method is 200µL. The is recommended for this.
standard 8125 has a 5µL loop but it Cat. No. Description
can be ordered with any loop 7410 Sample injection valve
attached. A 2.5µL full scale syringe is available for high accuracy and 7410-070 0.5µL Loop disc
precision in partial loading. 7410-071 1µL Loop disc
Valve accessories can be found on pages 180-183. 7410-072 2µL Loop disc

HPLC Accessories
7410-073 5µL Loop disc
Cat. No. Description
7012 Loop filler port
8125 Micro-scale injector with 5µL loop
9013 Needle port
87942 2.5µL RN syringe
7770-01 Replacement needles, 6/pk

2 1 2 1
Model 7520 Internal Pump Sample Pump Sample
Sample Loop Sample Loop
Sample Chamber Micro-Scale Injector
The Model 7520 uses a small hole Waste Column Waste Column
drilled in a flat rotor as an internal 4 5 4 5
sample chamber for precise sample
injection. The rotor, which is Position
PositionAA(LOAD)
(LOAD) Position
PositionBB(INJECT)
(INJECT)
sandwiched between two stators, is Flow paths of the load and inject positions of model 7410.
available in 0.2, 0.5, and 1µL
volumes; 0.5µL is standard. The
7520 requires the complete-filling
method of loading the internal
sample chamber. Excess sample is
required to completely flush mobile
phase from the chamber.
The schematic diagram shows the rotor (drawn as if it were a slider) Sample Needle Port
Sample Needle Port
in the LOAD position. The sample chamber is loaded by inserting a
syringe needle into the built-in needle port. The space between the Inlet Stator Sample Chamber Sample Chamber

needle tip and rotor surface contains only 0.3µL so there is very little
sample wastage. Rotating the handle to the INJECT position places Pump Pump

the sample chamber into the mobile phase stream. Rotor

The outlet stator passage is 0.13mm (.005 inch) diameter. A column Waste Waste

connecting tube of matching I.D. is supplied installed in the outlet Outlet Stator
port. The 1/16 inch O.D., 5cm long tube makes it easy to connect Column
Column
microbore columns of various designs.
Valve accessories can be found on pages 180-183.
Position
PositionAA(LOAD)
(LOAD) Position B B(INJECT)
Position (LOAD)
Cat. No. Description
7520 Internal sample chamber micro-scale injector Flow path of model 7520 internal sample chamber micro-scale injector.
7520-011 0.2µL Rotor
7520-012 0.5µL Rotor
7520-013 1.0µL Rotor

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 Rheodyne Manual Injector and Switching Valves
3725 Preparative Scale Injectors Two-Position Switching Valves
• 1/8" tubing ports; 1mm flow passages • Typical applications: column switching, sample
• Flow rates: 10 to 800 mL/min enrichment, sample clean-up
• Continuous-flow design; prevents pump shutdown The versatile two-position, six-port and ten-
• 100µL to 20mL volume range port valves are available in 1/16" and 1/8"
Rheodyne prep injectors for port sizes, and 316 SS and PEEK versions.
HPLC put big samples into big These valves redirect flow among columns
columns. They offer all the during the chromatographic run. They are
versatility and convenience of also useful for selecting between two
our analytical injectors, plus the columns. Each end of the off-line column is
ability to use columns with independently sealed instead of connected
diameters up to 10cm. together head-to-tail. Independent seals
produce less shock to the column if the valve
For biotech work, we provide a
switches before all the pressure leaves the
model with non-metal passages
column. A ten-port valve can often
of PEEK polymer. For general
accomplish the same operation that requires two six-port valves.
work, we offer a model that has
stainless steel passages to Model 7010 and 9010 sample injectors can convert to a six-port
accommodate all solvents. switching valve functionally identical to model 7000 by removing
the loop. Models 7010 and 9010 come standard with a 20µL sample
You can inject any volume from 100µL to 20mL by loading sample
loop. The loop is filled by flowing sample into either port 5 or 6
HPLC Accessories

from a syringe into a needle port in the handle. Flow rates can be as
while in the LOAD position. At least two loop volumes should be
high as 800mL per minute because the 1/8 inch tubing has low
used. The model 7012 loop filler port or 9013 needle port is
resistance and because the flow continues uninterrupted when you
recommended for this.
switch to INJECT thereby protecting the pump from over-pressure.
Valve accessories can be found on pages 180-183.
High pressure ratings permit you to use columns with very small
particles. The rating of the non-metal model is 276 bar (4,000 psi) Two Position, Six Port Switching Valves
and that of the stainless steel model is 345 bar (5,000 psi). The Cat. No. Description
needle port and syringe are never exposed to high pressure. They are 7000 Switching valve, SS, 1/16"
automatically isolated when you turn to INJECT. 7000L Switching large bore valve, SS, 1/16"
Both models are available in an “i” version with a built-in position 3000-038 Switching valve, SS, 1/8"
sensing switch (reed switch). Both models are supplied with a 10mL 3000 Switching valve, PEEK, 1/8"
loop and 1/8 inch fittings for all ports. RheFlex PEEK fittings must Two Position, Ten Port Switching Valves
be used with the PEEK injector. A port adapter (optional accessory) 7610-400 Switching valve, SS, 1/16"
can be used to connect 1/16 inch tubing. 7610-600 Switching valve, PEEK, 1/16"
Valve accessories can be found on pages 180-183. Sample Injector Valves
Cat. No. Description 7010 SS injector (complete loop filling only)
3725 PEEK Preparative injector 9010 PEEK injector (complete loop filling only)
3725i PEEK Preparative injector w/sensing switch 7012 Loop filler port
3725-038 SS Preparative injector 9013 Needle port
3725i-038 SS Preparative injector w/sensing switch

Waste Waste Two Column Selection Flow Diagram

6 6 Column A Column A

1 Waste 1 Waste
5 5 Sample Injector Sample Injector
1 6 1 6
Needle Port
2 2
2 5 2 5
4 Needle Port 4
Pump 3 Pump 3
3 4 3 4

Column Column Detector Detector

Column B
Column B
PositionAA(LOAD)
Position (LOAD) PositionBB(INJECT)
Position (INJECT)
Position A Position B
Flow paths of the load and inject positions of the anayltical, micro-scale
and preparative sample injectors, 7725, 9725, 8125 and 3725.

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 Rheodyne Manual Switching Valves

Column selection Sample clean-up

Three-Way Switching Valves 6-Position Selection Valves

Two-position, six-port valves with a double three-way switching The Model 7060 is a manually operated selector valve. This seven-
pattern are available in 1/16" and 1/8" port sizes, and 316 SS and port valve has a common (center) port that connects with one of six
PEEK versions. positions. A spring loaded detent mechanism allows continuous
Cat. No. Description rotation in either direction and insures that the shaft “falls into” each
7030 3-way SS switching valve, 1/16" position at the precise 60° spacing.
7030L 3-way large bore SS switching valve, 1/16" For column selection, an injector outlet is connected to the common
9030 3-way PEEK switching valve, 1/16" port of the 7060 which directs the flow to one of up to six columns.

HPLC Accessories
3030-038 3-way SS switching valve, 1/8" However, it is useful to use the sixth port for a bypass/flush-out tube.
3030 3-way PEEK switching valve, 1/8" A second 7060 is placed at the column outlets to direct the effluent to
the detector. Note that two separate 7060 valves are required.
The internal passages of the 7060 are 0.41mm I.D., with a total
internal volume less than 2µL.
Cat. No. Description
7060 6 position selection valve, SS, 1/16"
7060L 6 position L-bore selection valve, SS, 1/16"
9060 6 position selection valve, PEEK, 1/16"
3060-038 6 position selection valve, SS, 1/8"
3060 6 position selection valve, PEEK, 1/8"
Electrically Actuated Valves
Four-line BCD code for random access to any position
6 6 EV700-105 6 position selection valve, SS, 1/16"
1 1
EV750-105 6 position selection valve, PEEK, 1/16"
5 5

2 2 Six Column Selection Flow Diagram

4 4
3 3 Pump
1.00
Sample
Position A Position B Injector

1
2
Model 7040 4-way Valve 6
7 5
The Model 7040 is a six-port two-position valve. The internal flow 3
4
passages are identical to the Model 7000 but the external loop across
ports 1 and 5 converts it functionally to a 4-way valve. It exchanges
two streams: 2 -3 and 4-6 to 2-6 and 4-3. 1 2 3 4 5 6

Cat. No. Description

7040 4-way switching valve, SS, 1/16"
1
7040L 4-way large bore switching valve, SS, 1/16" 2 6

5
3
4

Detector
Six column selection using two model 7060 switching valves

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 Rheodyne Accessories: Rotor Seals, Stators and Kits
Stators
Cat. No. Description
3725-006 for 3725,3710-038,3000-038,3030-038, PEEK
3725-085 for 3725-038,3710-038,3000-038,3030-038
7010-040 for 7125, 7010, 7000, 7030, 7040
7010-040R Resurfaced stator*
7010-066 for 7125-081, 7010-087
7060-039 for 7060, 7066
7410-041 for 7410, 7413
7610-048 for 7610-600
7725-010 for 7725
8125-098 for 8125, 8126
9125-043 for 9125, 9010, 9030, 9725
9060-016 for 9060
Stator Face Assemblies:
Rotor Seals and Stators 7125-067 for 7125, 7126
7725-026 for 7725
The rotor seal is the polymeric disc that makes a high pressure seal 8125-074 for 8125, 8126
against the stator. The seal wears with use and is one of the only 8125-094 for 9125, 9010, 9030
parts that may need routine replacement. Stators need replacement 9060-015 for 9060
*
only if the ports or sealing surfaces become damaged. With Stator Exchange
Miscellanious Replacement Parts
HPLC Accessories

Vespel blend rotor seals have an operating range from 0 to 10. Tefzel
blend and PEEK blend rotor seals have a pH range from 0 to 14. 7125-008 Needle guide (for analytical front loading injectors)
Strong oxidizing acids such as concentrated nitric and sulfuric are not 7010-015 Isolation seal
compatible with PEEK.
Stators are available in 316 SS and PEEK. Our materials of
construction have been researched and selected for their physical and
mechanical strength.

Vespel Rotor Seals (pH 0-10)

Cat. No. Description
7010-039 for 7010, 7000, 7040
7030-003 for 7030
RheBuild® Kits
7060-070 for 7060, 7066
7125-047 for 7125, 7725 Cat. No. Description
7410-038 for 7410 3725-999 for 3725, 3725i, 3725-038, 3725i-038
7413-013 for 7413 7010-997 Kit to include stator for 7010
8125-038 for 8125, 8126 7010-999 for 7010, 7000
Tefzel Rotor Seals (pH 0-14) 7125-999 for 7125, 7126
7010-071 for 7010, 7010-087, 7000, 7040 7125Ti-999 for 7125-081
7030-015 for 7030 7410-999 for 7410
7060-074 for 7060, 7066, 9060 7520-999 for 7520, 7526
7125-079 for 7125, 7725 7725-999 for 7725, 7725i, 7726
7410-075 for 7410 7750-999 for 7750TPMV series
7413-045 for 7413 8125-999 for 8125, 8126
8125-097 for 8125 9010-999 for 9010, 9040
9010-051 for 9010 9125-999 for 9125, 9126
9125-082 for 9125, 9725 9725-999 for 9725, 9725i, 9726
9750-999 for 9750TPMV series
Tefzel Rotor Seal pH Upgrade Kits (pH 0-14) Contents of kit for front-loading injection valves: rotor seals, stator face
Kit includes:Tefzel rotor seal and 2 hex keys. assembly, isolation seal, needle guide, needle port cleaner, 2 hex keys,
7010-998 for 7010, 7000, 7040 operating instructions, and mini manual. The 7520-999 contains an inlet
7125-998 for 7125, 7126 stator, rotor, needle guide, needle port cleaner, hex key, rotor removal tool,
7410-998 for 7410 and operating instructions.
8125-998 for 8125, 8126 7010-Stator Face Upgrade
PEEK Rotor Seals (pH 0-14) Recommended for Thermo/Spectra Physics and Agilent instruments.
3710-008 for 3000, 3000-038, 3710, 3710-038 With this conversion, the rotor seal contacts the ceramic stator face
3030-005 for 3030, 3030-038 assembly providing for indefinite stator life.
3060-001 for 3060, 3060-038 Cat. No. Description
3725-018 for 3725, 3725-038 7010-996 7010-Stator face upgrade
7610-011 for 7610-400, 7610-600 Stator face upgrade kit includes: Rotor, stator face assembly, isolation
9125-095 for 7125, 7725, 9125, 9725 seal, stator ring, stator screws, 2 hex keys and manual.

180 1-800-822-5242 • in MN 952-431-6000 • www.chromtech.com • [email protected]

 Sample Loops for Rheodyne Valves
Upchurch SS Loops for 7125 and 7010
Cat. No. Sample Loop Volume
1850 5µL sample loop
1851 10µL sample loop
1853 15µL sample loop
1852 20µL sample loop
1856 25µL sample loop
1857 30µL sample loop
1858 35µL sample loop
1859 40µL sample loop
1855 50µL sample loop
1860 100µL sample loop
1861 150µL sample loop
1862 200µL sample loop
1864 250µL sample loop
Rheodyne Sample Loops 1867 300µL sample loop
1865 500µL sample loop
1871 1mL sample loop
External loops are supplied with unattached fittings so the tube can be completely bottomed in 1872 2mL sample loop
the injector port before the ferrule is swagged on. This allows for different port depths. The 1875 5mL sample loop
depth of the tubing holes may vary slightly from port to port and from valve to valve. It is 1876 10mL sample loop
good practice to label loops so that they will be replaced in the same orientation and the same 1877 20mL sample loop

HPLC Accessories
valve. PEEK loops do not require this precaution because the ferrule can slide and reposition
itself along the tube when the fitting is reinserted into a port. Upchurch SS Loops for 7725 and 7725i
1950 5µL sample loop
Size designations are nominal. Sizes vary from nominal values usually less than 15%. 1951 10µL sample loop
Accuracy and precision in partial filling require that no more than half the loop volume be 1952 20µL sample loop
loaded. Precision in complete filling requires that at least two loop volumes be loaded. 1955 50µL sample loop
1960 100µL sample loop
SS Loops for 7125 and 7010 Injectors PEEK Loops for 9725 and 9010 Injectors 1962 200µL sample loop
Cat. No. Sample Loop Volume Cat. No. Sample Loop Volume 1965 500µL sample loop
7020 5µL sample loop 7755-015 2µL (9725 only) 1971 1mL sample loop
7021 10µL sample loop 9055-020 5µL sample loop 1972 2mL sample loop
7022 20µL sample loop 9055-021 10µL sample loop 1875 5mL sample loop
7023 50µL sample loop 9055-022 20µL sample loop 1876 10mL sample loop
7024 100µL sample loop 9055-023 50µL sample loop 1877 20mL sample loop
7025 200µL sample loop 9055-024 100µL sample loop
7026 500µL sample loop 9055-025 200µL sample loop Upchurch PEEK Sample Loops
7027 1mL sample loop 9055-026 500µL sample loop 1800 2µL sample loop
7028 2mL sample loop 9055-027 1mL sample loop 1801 5µL sample loop
7029 5mL sample loop 9055-028 2mL sample loop 1802 10µL sample loop
9055-029 5mL sample loop 1803 20µL sample loop
SS Loops for 7725 and 7750 Injectors 1805 50µL sample loop
7755-020 5µL sample loop 9055-033 10mL sample loop
1810 100µL sample loop
7755-021 10µL sample loop PEEK Loops for 3725 Injectors: 1811 150µL sample loop
7755-022 20µL sample loop 3055-018 2mL sample loop 1812 200µL sample loop
7755-023 50µL sample loop 3055-019 5mL sample loop 1814 250µL sample loop
7755-024 100µL sample loop 3055-023 10mL sample loop 1815 500µL sample loop
7755-025 200µL sample loop 3055-025 20mL sample loop 1820 1mL sample loop
7755-026 500µL sample loop Titanium Loops 1821 2mL sample loop
7755-027 1mL sample loop 7055-021 10µL sample loop 1822 5mL sample loop
7755-028 2mL sample loop 7055-022 20µL sample loop 1825 10mL sample loop
7755-029 5mL sample loop 7055-023 50µL sample loop 1826 20mL sample loop
SS Loops for 8125 Injector 7055-024 l00µL sample loop
8020 5µL sample loop 7055-025 200µL sample loop
8021 10µL sample loop 7055-026 500µL sample loop
8022 20µL sample loop 7055-017 lmL sample loop
8023 50µL sample loop 7055-028 2mL sample loop
SS Loops for 3725-038 Injectors 7055-029 5mL sample loop
3065-018 2mL sample loop
3065-019 5mL sample loop
3065-023 10mL sample loop
3065-025 20mL sample loop

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