Scribd
Upload
23 views

Lab Week 6 Lab Challenge

Academic

Uploaded by

kevvndaniels
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
23 views

Lab Week 6 Lab Challenge

Academic

Uploaded by

kevvndaniels
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 3

Lab Week 6 Lab Challenge

Transcription and Translation:


From a DNA code to a protein message
For this lab challenge you will work in a group to decode a message written
as a DNA sequence, but first transcribing it to RNA, and then translating it to
an amino acid sequence where the single-letter amino acid abbreviations will
spell out a kind-of humous message. Each member of the group will then
suggest a random base substitution mutation in the DNA sequence and you
can see who disrupted the message the most. Finally, you can discuss with
the class how you found the correct translation start site, and the impact of
your mutations.

Your group will work on either the eukaryotic DNA sequence or the
prokaryotic sequence.

Be sure to read the Important Reminders associated with each sequence.

PROKARYOTIC DNA SEQUENCE


Sequence Provided: Coding strand of the Operon
Promoter sequence in DNA: 5’TATAAT (precedes transcriptional
start site)
Ribosomal Binding Sequence in mRNA: 5’ AGGAGG

5’-
GACAATATAATGCGATTTACGATTGCCGTATGTAAGGAGGTACCATGTATAGC
ATGGCGCTGCTGTGCCATATTCTGGATCGCGAAGCGGATAGCGCGACCCATA
TTAACCCGGCGCCGGAACGCTGGATTACCCATACCCATATTTGCAAAGGCCT
GGCGAGCAGCGAAAGCTGAGATTTACGATTGCCGTATGTAAGGAGGGCATA
TGGCGCGCAGCATTAGCTTTGCGCGCGCGTGGGCGTATTGGCATATTCTGGA
AGAAGCGCGCACCCATATTAGCGTGGAACGCTATAACGAAGCGCGCTAAGTA
GGCCATGC – 3’

Important Information about the Prokaryotic DNA sequence:


1) This is the coding strand in DNA, so you don’t need the reverse
complement of this sequence for transcription.
2) First: transcribe this DNA to mRNA starting with the first nucleotide
immediately after the Promoter sequence provided (near the 5’ end of
the DNA) and transcribe to the end of the DNA sequence provided.

3) This is an Operon, so there will be more than one gene (translation


start sites).
4) In the mRNA: find the first AUG after each Ribosomal Binding Sequence
(this is the Shine-Delgarno sequence)… these are the start codons for
the proteins, and translate using the genetic code.

Step 1

5’- GACAATATAATGC…-3’
promoter sequence (TATAAT), is highlighted and at position 6 -11

mRNA Sequence: 5'-


GCGAUUUACGAUUGCCGUAUGUAAGGAGGUACCAUGUAUAGCAUGGCGC
UGCUGUGCCAUAUUCUGGAUCGCGAAGCGGAUAGCGCGACCCAUAUUAAC
CCGGCGCCGGAACGCUGGAUUACCCAUACCCAUAUUUGCAAAGGCCUGGC
GAGCAGCGAAAGCUGAGAUUUACGAUUGCCGUAUGUAAGGAGGGCAUAU
GGCGCGCAGCAUUAGCUUUGCGCGCGCGUGGGCGUAUUGGCAUAUUCUG
GAAGAAGCGCGCACCCAUAUUAGCGUGGAACGCUAUAACGAAGCGCGCUA
AGUAGGCCAUGC - 3'

Step 2

AGGAGG found at position 24-29


The AUG codon after AGGAGG at position 34-36

Step 3:

…GACAATATAATGCGATTTACGA… original at position 15


…GACAATATAATGCGGTTACGA…. mutated changed A to G

Step 4
GCGAUUUACGA. . -3 mRNA

Step 5

start codon remains the same AUG.

Class discussion topics:


Did you find the message(s)? I was able to find the mRNA message as well as
translation start sites
Did you splice correctly, did you try alternative splicing? I chose prokaryotic
DNA, so splicing was not perfomed
Where did your mutations hit? at position 15 of the DNA sequence, changing
an A to a G.
How did your mutations in the DNA impact the protein message? It did not
alter the AUG start codon

You might also like