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Lactase Enzyme Activity With Data Analysis 2021 Temperature

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Chunting Emmanuel
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89 views5 pages

Lactase Enzyme Activity With Data Analysis 2021 Temperature

Uploaded by

Chunting Emmanuel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Name: ____________________________________________ Date: ___________ Period: ______

Lactase Enzyme Activity


with Data Analysis
Simulation at https://exchange.iseesystems.com/public/jon-
darkow/lactase-enzyme-simulation-with-data-analysis/
index.html#page1

https://sites.google.com/site/biologydarkow/enzymes/lactase-
enzyme-simulation

Pre-Lab

Read the “Home” tab.

1. What type of macromolecule is an enzyme?

2. What is the main function of an enzyme?

3. What 3 factors will you be able to manipulate in this model?

4. How many times should you repeat each test?

5. How many variables should you manipulate at a time?

Read the “Background” tab.

6. What is the enzyme in this lab? What suffix generally indicates that a molecule is an enzyme?

7. What is the substrate of this enzyme? What type of macromolecule is it? What suffix generally
indicates this type of molecule?

8. What is the consequence of not having lactase?

9. Define reaction rate. How do enzymes affect the rate of a reaction?


10. Why is it important for cells to have chemical reactions happen quickly?

11. How many substrates does on enzyme usually bind to at a time?

12. Why does the rate of reaction plateau at some point for every enzyme-catalyzed reaction?

13. Look at the diagram of the reaction. Is lactose a monosaccharide, a disaccharide, or a polysaccharide?
How can you tell?

14. Are glucose and galactose monosaccharides, disaccharides, or polysaccharides? How can you tell?

15. Explain how the structure of an enzyme relates to its functional ability to catalyze chemical reactions?

16. Why would being able to produce lactase as an adult be an evolutionary advantage?

Read the “Explore Model” tab.

17. What are the units that we will measure to find the rate of reaction?

18. As this reaction proceeds, what will happen to the concentration of lactose? (Increase/Decrease/Stay
the same)?

19. As this reaction proceeds, what will happen to the concentration of glucose? (Increase/Decrease/Stay
the same)?

20. As this reaction proceeds, what will happen to the concentration of lactase? (Increase/Decrease/Stay
the same)?

21. What factors in this simulation will determine the speed at which the reaction occurs?

Simulation Option 1: Temperature


Start with the initial lactose at 500 mg/dL and the pH at 7. Do not vary these parameters while testing for
temperature; they are environmental controls. The temperature is in degrees Celsius. Vary the temperature
by 20oC. Run each experiment 5 times (for reliability in the results.)

In order to see how much glucose has been produced in 3 minutes in the simulation, click and hold on the
graph. The number of mg/dL of glucose produced will appear underneath the graph under each Run number.
Record these amounts in your Table 1.

Table 1: Amount of glucose produced at each temperature 3 minutes after addition of lactase (mg/dL)
Temperature 0oC 20oC 40oC 60oC 80oC
Trial 1
Trial 2
Trial 3
Trial 4
Trial 5

To calculate the rate of reaction between 0 and 3 minutes, divide the amount of glucose produced by 3, and
record in Table 2. The units will be mg/dL of glucose per minute. Calculate the mean, the standard deviation,
and SEM (standard error of the mean).

Table 2: Rate of Reaction at each temperature (mg/dL of glucose/min)


Temperature 0oC 20oC 40oC 60oC 80oC

Trial 1

Trial 2

Trial 3

Trial 4

Trial 5

Mean
Standard
Deviation
Standard Error of
the Mean (SEM)

2. Graph the mean rate of reaction at each temperature. Connect the dots to make a curve. Make sure your
axes are labeled correctly and you have an appropriate title. Include error bars to illustrate 95% confidence
(i.e., sample mean ± 2 SEM).
3. Write a sentence summarizing the effect of temperature on enzyme activity.

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