3334 Problem Set 2B Buffers Last Name__________________________

Ll

Fall 2024 Jessica

First Name__________________________

5. How many grams of solid tris-(hydroxymethyl)-aminomethane (FW 121) and how

PH = DRA +
109
[A-2 many milliliters of 1.0 N HCl must one use to prepare 500 ml of “tris” buffer, 0.05 M,
CHAS pH 7.6? pka of tris = 8.4. Crystalline tris exists as: weigh out 3 0259 of tris and
8 4 1099A-3 9 of tris
.

.6
7 = + : 0 SMOl 0 51 0 025 MO1 ·
1219
measure out
. .
:
21 6 MC of All then
.
-
.

CHA] L Mol
.

0 8 [A CHAS = 3 0259 [A -] add Heo to get to sooms of total

109
«
.
-

= + +
1 M H (1
(HOCH2)3CN H3 (HOCH2)3CNH2 + H
.

: IN H(1
[HA] Volume
0 05 MHC1 0 S NHC

, so
=
A-]
. .

0 . 158 = 2SM) : 21 . OML

0. S1 = 0 02S MN Of HCl
[HA] .

ISCHL Of HCl
& to 100 % dissociate from CAS to CHAS

PH =
pa +
106. Prepare 1.0 L of 0.1 M citrate buffer, pH 4.95 from monosodium citrate (FW 214)
4 95
. = 4 75. + 1099A-S (9.) and disodium citrate (FW 236). Citric acid pka1 = 3.10, pka2 = 4.75, pka3 = 5.40.
1 58
weigh out 8 299 of Monona citrate
CHA]

CHAS:ISO= On
CA -] CA-s =
.

x0 7 M = 0 0672M
0 2 =
109
. . .

. 58
2
.

CHA]
0 0612 M
and 14 449 of PiN citrate then add
1 01 0 0012 MOL
.
-

(A ] =
- .

1. 58
.
.

water to get to 11
=

CHA] m 0 0612MOl 40 839m0 2149 8 299

2309
·

14 449
=
·
: .

.
. .

7. Prepare 1.0 L of 0.1 M citrate buffer, pH 4.95, from crystalline citric acid (FW 210)
(10.) and 1.0 M NaOH.
weigh out 219 citoic acid crystalline
work on next pages and me a sure out 161 24m1 of NaOH .

then add water to 1

8. Prepare 1.0 L of 0.2 M citrate buffer, pH 5.3. Assume the availability of crystalline
(11.) citric acid (FW 210), monosodium citrate (FW 214), disodium citrate (FW 236),
trisodium citrate (FW 258), and 1.0 M NaOH. Use pk values in problem 9. Structure:

HOOC-CH2-COH-CH2-COOH
½
weigh out 429 of crystalline citric acid.

COOH Then Measure out 488 SeML of NaOH.

·

add water to get to 11.

work on next pages

-3
9. Calculate the plasma concentration of PO4 when plasma pH = 7.4 and
-2
concentration of plasma HPO4 = 0.001 M. Phosphoric acid pKa1 = 2.2, pKa2 = 7.2,
pKa3 = 12.4.
[P043] 1 58 10-3 M = .
.

Nork on next pages

10. Describe three (A., B., and C.) different ways to prepare 100.ml of 50.0 mM CHES
 buffer at a pH of 8.8 using CHES acid (HA, pKa = 9.39, FW 207), Na salt of CHES
(A-, FW 229), 2.5 M HCl, and 0.125 M NaOH.

A .
Using CHES dcid ,
Method I would be used .
Henderson- hasselbach equation would be used for
the ratio ,
then a can be determined by multiplying ratio by concentration to get moses of
CHAS Or CA's
present ,
then multiply by Mw to get total of CHAS or <A) present

B Using Hel method would be used bic it is a strong acid henderson-hasselback

,

find
.

Use to the
ratio of CHAS and
.

<As in the solution . We then multiply the

# 7 . 1) of 0 . Incitrate buffer ,
DH = 4 95.

,
from crystalline citoic Acid (FW210) $1 OM NOOH
.

need 100m) to fully deprotonate

Citrate-NaOH) :
MOMONacitrate :
He DRA = 3 .
10

Monona citrate :
NaOH dina citrate +
#28 DRA =
4 75.

DiNacitrate + NOOH 1 triNacitrate + H20 DRA = 3 40 .

[A ]
-

DH = DKd +
109 10 1M((11)
.
= 0 .
1 mole of citric acid
CHA]

n 10 1 . (12109) : 21 09 of.
citric acid

4
[A -]
. 95 = 4 75 .
+
109
CHA]

3 DKA 3H deprotonate
+
* =
to

0 2 .
=
logA s given 1 O M
. NaOH

1 0 M
.
= 1 ON
.
= 1000 MN = 1000M/

[A J
-

1 58
.
=
3H
+
to deprotonate at 0 I M
. concentration
[HA]

20 I M NAOH per
+
. H

CA-S = 1 . 58 Gratio : in buffer 4 0 / M = 0 /N = 100MN : 100 M/

, . .

2 . 58 1 . 50 iS < A

* NaOH deprotonate Monona citrate to dia citrate

1 58
CAS =
.

100M) = 61 .
24M) of NaOH to deprotonate Monona to did
2 .
50

Total NaOH = 161 24 ML

. =

total crystalline :
119
# 8 .
11 of 0 2M . citrate Duffer
,
DH = S 3 .
.
citric Acid crystalline =
2109 .

MonoNacitrate =
2149 .
DiNa citrate =
2369 .
Trina citrate :
2509 .
WIL OMNAOH
.

Citrate +
NAOH: :
Monona citrate :
He DRA ,
= 3 .
10

Monona citrate :
NaOH dina citrate +
#28 DRAc : 4 75
.

DiNacitrate + NaOH 1 triNacitrate + 120 PK & 3

= 5 48
.

[A ]
-

DH = DKA +
109 10 .
2 M1 c 1 .
01) = 0 2 moles
.

CHA]

20 . [ MOles((2109) = 429

5 . 3 = 5 . 40 +
189[A]
[HA]
>
IM NACH = IN NaOH

-0 1 .
: 10g[A-S >
-
0 2 M NaOH
.
= 0 2 NNAOH
.

[HAS

>
-
total : 0 . N NaOH

[A-J
0 794
.
= 0 2N per reaction
CHA]
.

200 MNNACH : 200 ML NACH

0 794
< A- 3
.

1 794
.

0. 794 . LOOMI NAOH to partially dissociate CHAS to < As

1 . 794

88 51ML NaOH needed

.
for DKA : S 48
.

↑ 0 + 91 : 488 52 ML of NaOH
.
9. Calculate plasma concentration of poys- When plasma PH = 7 4
.
.
concentration

of Plasma HPO42 = 0 .
001 M .
PRA ,
=
2 .
2 . DK&c = 1 2 .
, PRA = 12 4 .

Is PO4 +
NADH""HePOp :
H18 PKD = 1 .
1

HePO4" + N9OH 1 :
HPOy" +
He8 PRA = 7 2 .

H POp" + NOOH 11 1043 + H2O DRA : 12 .

4

Dkdc : Closest 10 DH PRA3 : Dissociation of HPO4"10 POp3-

[A] CA S
-

DH = DRA +

109CHA] DH =
pKa +
109
CHA]

A S
109CA-s
-

. 4
7 = 7 2
.
+ 109 1. 4 = (2 4 .
+

20 001] . 50 001] .

"As
0 2 .
=
10g 5 = 109[A
20 . 0015 20 001] .

[A'S 5 [A S
18
-

1 58 .
= 1 .

0 08 .
< 0 0013
.

3
10-oM
-

C A S
-

= 1 5 % 10
.
M
<
A- ) = 1 .
0 .