Bioprocess Syllabus
Bioprocess Syllabus
Course Outcome
Students will be able to assess nutritional status and apply the knowledge in understanding the
metabolism and nutrient functions.
UNIT I
Introduction to Bioprocess Technology: History and Scope- Bioreactor: Design, parts and
accessories, functions- Modes of Operation of fermenter – Batch & continuous - Types of
reactors - Bubble column, Fluidized bed reactor, plug flow reactor.
UNIT II
Fermentation media design, sterilization and media requirement for industrial fermentation, Main
parameters to be monitored and controlled in fermentation processes, aerobic and anaerobic
fermentation processes. Development and scale up of bioreactors for production of biological
products. Immobilization – Types of immobilization, various methods - Applications of
immobilized enzyme technology.
UNIT III
Downstream processing: Cell disruption methods for intracellular products, removal of
insolubles, biomass (and particulate debris) separation techniques, flocculation and
sedimentation, centrifugation and filtration methods. Enrichment operations: Membrane – based
separations. Product finishing: precipitation/crystallization, mixing, dialysis, distillation and
drying.
UNIT IV
Production of microbial enzymes (Amylase, protease and pectinase) applications, production of
organic solvents (Ethanol, Methanol) – production of organic acids (Citric acid, Acetic acid) -
Single cell protein production – Spirulina, Yeast, Actinomycetes protein. Beverages production –
Beer and Wine.
UNIT V
Processing of Milk – Pasteurization and homogenization - Modifying milk composition –
Production of milk products – Curd, cheese, yogurt, and flavoured milk. Bakery products –
Bread making. Probiotics and Role of Food technology in bio-defense programs.
References:
1. Shuler, M.L. and Kargi, F. 2008. Bioprocess engineering – Basic concepts. Pearson Education.
2. M.L. Srivastava., 2010. Fermentation Technology, Narosa Publications.
3. Pauline M. Doran., 2009.Bioprocess Engineering Principles. Academic Press Inc.,
4. El-Mansi& Bryce C.F.A., 2007. Fermentation Microbiology and Biotechnology., 2nd edition,
Taylor and Francis Publishing.