Santosh Dubey@bhu Ac in
Santosh Dubey@bhu Ac in
Santosh Dubey@bhu Ac in
1. Professor, Centre of Advanced Study in Botany, Banaras Hindu Oct. 10, Till Date
University, Varanasi, U. P. India 2017
Teaching Interest: General Microbiology, Molecular Biology,
Environmental Microbiology, Microbial Genomics, Agricultural
Microbiology
Research Interest: Molecular Microbiology, Nanobiotechnology (Green
synthesis of MNPs mediated by cyanobacteria and bacteria), Genetic
Engineering, Environmental Microbiology & Biotechnology
Honours / Awards
Academic Expertise:
1. Study the resistance mechanisms in heavy metal (Pb, Cd, Hg) metalloid (As, Te,
Se) and organotin (TBT, DBT) resistant bacteria.
2. Study bioremediative potential of heavy metal/ metalloid/ organometal resistant
bacteria.
3. Development and characterization of luminescent bacterial sensors for toxic
metals/metalloids/ organometals using recombinant DNA technology.
4. Bioprospecting of terrestrial and marine bacteria for industrially important
enzymes viz. protease, cellulase, lipase, amylase (culture dependent and metagenomic
approach), EPS and antimicrobial organic metabolites.
5. Biosynthesis of Selenium, Tellurium, Copper, Zinc and Silver nanoparticles using
marine bacteria and cyanobacteria, their biological characterization and
applications.
F. Edited book : 01
G. Chapters in Indian/International Edited Books :07
H. Research paper in conference proceeding 01
Publications
1. Dubey, S. K. and L. C. Rai 1987. Effect of chromium and tin on survival, growth, carbon
fixation, heterocyst differentiation, nitrogenase, nitrate reductase and glutamine synthetase
activities of Anabaena doliolum. Journal of Plant Physiology 130: 165-172. (IF : 3.549, C:
31)
2. Rai, L.C. and S. K. Dubey. 1989. Chromium toxicity in cyanobacteria: Possible role of carbon
sources in toxicity amelioration. Microbios 55: 193-203.(IF:0.8; C:07)
3. Rai, L. C. and S. K. Dubey. 1989. Impact of chromium and tin on a nitrogen fixing
cyanobacterium, Anabaena doliolum : Interaction with bivalent Cations. Ecotoxicology and
Environmental Safety 17: 94-104. (IF:6.291; C:19)
4. Dubey, S.K. and L. C. Rai 1989a.Toxicity of chromium and tin to Anabaena doliolum:
interaction with sulphur containing aminoacids and thiols. Biology of metals 2: 55-60. (IF:
2.949; C: 11)
5. Dubey, S.K. and L. C. Rai 1989b. Protective effects of certain natural and synthetic
complexans on the toxicity of chromium and tin to nitrogen fixing cyanobacterium, Anabaena
doliolum. Biomedical and Environmental Sciences 2: 271-281.(IF:3.118 ; C:02).
6. Dubey, S. K. and L. C. Rai 1990. Impact of chromium and tin on a nitrogen fixing
Cyanobacterium, Anabaena doliolum: Regulation of toxicity by certain environmental factors.
Biomedical and Environmental Sciences 3: 240-249. (IF: 3.118 ; C: 13)
7. Rai, L.C., M. Raizada, N. Mallick, Y. Husainy, A. K. Singh and S. K. Dubey 1990. Effect of
four heavy metals on the biology of Nostoc muscorum. Biology of Metals 2(4) : 229-234.
(IF: 2.949; C: 42)
8. Dubey, S. K., and L. C. Rai 1990. Toxicity of chromium and tin to Anabaena doliolum:
interaction with bivalent cations. Biology of Metals 3: 8-13. (IF: 2.949 ;C: 04)
9. Rai, L.C., S. K. Dubey and N. Mallick 1992. Influence of chromium on some physiological
variables of Anabaena doliolum : interaction with metabolic inhibitors. Biometals 5: 13-16.
(IF: 2.949 ; C:15)
10. Dubey, S. K., Gangolli, S. S. and D. S. Holmes 1993. Development of biosensors to measure
metal ion bioavailability in environmental samples. Journal of North Eastern Geology 15:
88-94.(C:08)
11. Holmes, David S. , Dubey, Santosh K. and Seema Gangolli 1993. Development of
biosensors to measure metal ion bioavailabililty in mining and metal wastes. In:
Biohydrometallurgical Technologies Vol II (Torma, A. E., Apel, M. L. and Brierley, C.
L.).The minerals, metals and materials society, Philadelphia, USA, pp. 659 – 666. (C: 10)
12. Holmes, D. S., S. K. Dubey, and S. S. Gangolli. 1994. Development of biosensors for the
detection of mercury and copper ions. Environmental Geochemistry and Health 16: 229-233.
(IF: 4.609 ; C:31)
13. Dubey, S. K., Holmes, D. S. 1995. Review - Biological cyanide destruction mediated by
microorganism. World Journal of Microbiology and Biotechnology 11: 257-265. (IF:3.312;
C:159)
14. Roy, U., Dubey, S. K. and Bhosle, S. 2001. Characterization of exopolymers produced by
Sphingomonas sp. utilizing tributyltin chloride(TBTC). In: Proceedings of International
conference on industrial pollution and control technologies (ICIPACT-2001) held in
Hyderabad, A.P., India, pp. 639-642.
15. Dubey, S. K., Holmes, D. S., Rao, G. R. and Gangolli, S. 2002. Detection and measurement
of ultra-trace levels of mercury and copper by genetically engineered microbial sensors. In:
Recent Advances in Environmental Science (Ed. Hiremath, K. G.), Discovery Publishing
House, New Delhi, India pp. 245-255.
18. Dubey, S. K. and P.V. Bramhachari 2004. Cyanobacteria in heavy metal polluted
environment: Environmental Biotechnology perspective. In: Biotechnological Applications
of Cyanobacteria (Ed. Singh, K.), pp. 225-244.
19. P.V. Bramhachari and Santosh Kumar Dubey 2006. Rapid and specific detection of
luminous and non-luminous Vibrio harveyi isolates by PCR amplification. Current Science
90 (8): 1105- 1108. (IF: 0.9; C: 25)
20. Santosh K. Dubey, Tsutomu Tokashiki, and S. Suzuki 2006. Microarray mediated
transcriptome analysis of the tributyltin (TBT) resistant bacterium, Pseudomonas aeruginosa
25W in the presence of TBT. Journal of Microbiology 44:200-205. (IF: 3.422 ; C: 26)
22. P. V. Bramhachari, P.B. Kavikishor, Ranadheer Kumar, B. Rama Rao and S. K. Dubey
2007. Isolation and characterization of Mucus exopolysaccharide produced by Vibrio
furnissii strain VB053. Journal of Microbiology and Biotechnology 17 (1): 44-51. (IF:
2.351; C: 126)
23. R. Krishnamurthy, L. Cabral, Vidya, R. and Santosh Kumar Dubey 2007. Isolation and
biological characterization of a TBTC degrading marine bacterium, Vibrio sp. from Bombay
high oil field, India. Current Science 93(8): 1073-1074. (IF:0.9)
25. Fukushima, K., Dubey, Santosh K., and S. Suzuki 2009. Quantitative analysis of expression
of Tributyltin (TBT)-regulated genes in TBT resistant Pseudomonas aeruginosa 25W. In:
Interdisciplinary Studies on Environmental Chemistry-Environmental Research in Asia
(Eds. Obayashi, Y., Isobe, T., Subramaniam, A., Suzuki, S. and Tanabe, S.) pp. 163-166,
TERRAPUB Press, Japan. (C: 05)
26. P.V. Bramhachari and S. K. Dubey 2010. Biochemistry, Molecular Biology of Bacterial
Bioluminescence and Applications of Lux genes. In : Cellular and Biochemical Sciences
(Ed.Tripathi, G.), International Publishers, New Delhi, India.
27. Pandey, Anju, Naik, Milind M. and S. K. Dubey 2010. Organic metabolites produced by
Vibrio parahaemolyticus strain An3 isolated from Goan mullet inhibit bacterial fish
pathogens. African Journal of Biotechnology 9(42): 7134-7140. (IF:0.8; C: 33)
28. Pandey, Anju, Naik, Milind and Santosh Kumar Dubey 2010. Hemolysin,
protease and EPS producing pathogenic Aeromonas hydrophila strain An4 shows
antibacterial activity against marine bacterial fish pathogens. Journal of Marine
Biology 40: 1-7. (Impact Score : 0.75 ; C: 54)
29. Milind Mohan Naik and S. K. Dubey 2011. Lead – enhanced siderophore
production and alteration in cell morphology in a Pb- resistant Pseudomonas
aeruginosa strain 4EA. Current Microbiology 62: 409-414.(IF: 2.188; C: 94)
30. Pandey, Anju, Naik, Milind and Santosh Kumar Dubey 2011.Biological
characterization of marine fish pathogen, Acinetobacter sp. strain An2 producing
antimicrobial metabolites. Journal of Scientific and Industrial Research 70 : 135-
141. (IF: 1.056 ; C: 09)
31. Naik, Milind Mohan, Pandey, Anju and S. K. Dubey 2011. Bioremediation of metals
mediated by marine bacteria. In: Microorganisms in Environmental Management
-Microbes and Environment (Eds. Prakash, A., Satyanarayana, T. and B. N.
Johri), Springer. DOI :10.1007/978-94-007-2228-6. (C: 19)
32. Naik, Milind, Anju Pandey and Santosh Kumar Dubey 2012. Pseudomonas
aeruginosa strain WI-1 from Mandovi estuary possesses metallothionein to alleviate
lead toxicity and promotes plant growth. Ecotoxicology and Environmental Safety
79: 129-133. (IF:6.291; C:92)
33. Naik, Milind Mohan, Pandey, Anju and Santosh Kumar Dubey 2012. Biological
characterization of lead – enhanced exopolysacharide produced by a lead resistant
Enterobacter cloacae strain P2B. Biodegradation 23(5): 775-783.(IF:3.909; C:79)
34. Fukushima, K., Dubey, Santosh Kumar and Satoru Suzuki 2012. YgiW homo-
logous gene from Pseudomonas aeruginosa 25 W is responsible for tributyltin
resistance.Journal of General and Applied Microbiology 58:283-289.(IF:1.442;C:21)
35. Naik, Milind Mohan, Kashif Shamim and Dubey, Santosh Kumar 2012.
Biological characterization of lead resistant bacteria to explore role of bacterial
metallothionein in lead resistance. Current Science 103 (4):426-429. (IF:0.9; C:54)
36. Kunkalekar, R. K., Naik, M.M., Dubey, S. K. and A.V. Salkar 2012. Antibacterial
activity of Silver-doped manganese dioxide nanoparticles on multi-drug resistant
bacteria. Journal of Chemical Technology and Biotechnology 88 : 873-877.
(IF:3.174 ; C: 31)
37. Shamim, K., Naik, M.M. , Pandey, Anju and Santosh Kumar Dubey 2012.Isolation
and identification of Aeromonas caviae strain KS-1 as TBTC and Lead resistant
estuarine bacteria. Environmental Monitoring and Assessment 185: 5243-5249.
(IF: 2.513; C: 29)
38. Naik, Milind, Khanolkar, Dnyanada, and Santosh Kumar Dubey 2012. Lead
resistant Providencia alcalifaciens strain 2EA bioprecipitates Pb 2+ as Lead
phosphate. Letters in Applied Microbiology 56: 99-104. (IF: 2.858 ; C :50)
40. D’Costa, Brenda, Dnyanada Khanolkar and Santosh Kumar Dubey 2013. Partial
purification and characterization of metalloprotease of halotolerant alkaliphilic
bacterium, Bacillus cereus from coastal sediment of Goa, India. African Journal of
Biotechnology 12(31): 4905-4914. (IF: 0.8; C: 08)
41. Naik, Milind, Dnyanada, Khanolkar, Brenda D’ Costa and Santosh Kumar Dubey
2013. P-type ATPase and MdrL efflux pump mediated lead and multi-drug resistance in
estuarine bacterial isolates. Current Science 105(10) :1366-1372.(IF: 0.9; C: 13)
42. Milind Mohan Naik and Santosh Kumar Dubey 2014. Review - Lead Resistant
Bacteria:Lead resistance mechanisms, their applications in lead bioremediation and
biomonitoring. Ecotoxicology and Environmental Safety 98:1-7.(IF:6.291; C:200)
44. Dnyanada Khanolkar, Milind Mohan Naik and Santosh Kumar Dubey 2015.
Biotransformation of Tributyltin Chloride by Pseudomonas stutzeri strain DN2.
Brazilian Journal of Microbiology 45(4) : 1239-1245. (IF: 2.857; C: 07)
46. Dnyanada S. Khanolkar, Santosh Kumar Dubey and Milind Mohan Naik 2015.
Biotransformation of tributyltin chloride to less toxic dibutyltin dichloride and
monobutyltin trichloride by Klebsiella pneumoniae strain SD9. International
Biodeterioration & Biodegradation 104 : 212-218. (IF: 4.320 ; C: 15)
47. Naik, Milind M. and S. K. Dubey 2016. Lead and mercury resistant marine bacteria
and their application in lead and mercury bioremediation. In: Marine Pollution and
Microbial Remediation (Eds. Naik, M. M. and Dubey, S. K.), pp.29-40,Springer,
Singapore. (C: 15)
48. Naik, M.M. and Dubey, S. K. 2016. Marine Pollution and Microbial Remediation
(Edited book), Springer, Singapore.(C:58)
49. Sharma, Jaya , Kashif Shamim, Santosh Kumar Dubey, Ram Murti Meena 2017.
Metallothionein assisted periplasmic lead sequestration as lead sulfite by Providencia
vermicola strain SJ2A. Science of the Total Environment 579:359-365.(IF:7.963;C:36)
50. Praveen Kumar, M.K., Shyama, S.K., Shamim K., Dubey, S. K., D’ Costa, A.,
Sonaye, B. H., Kadam, S. and R. C. Chaubey 2017. Effects of gamma radiation on the
early developmental stages of Zebra fish (Danio rerio). Ecotoxicology and
Environmental Safety 142 : 95–101. (IF:6.291;C:20)
51.Shamim, K, Sharma, J. and S.K. Dubey 2017. Rapid and efficient method to extract
metagenomic DNA from estuarine sediments. 3 Biotech 7 :182-190.(IF:2.406;C:03)
52. Sharma, J., Shamim, K. and Santosh Kumar Dubey 2018. Phosphatase mediated
bioprecipitation of lead as pyromorphite by Achromobacter xylosoxidans. Journal
of Environmental Management 217: 754-761. (IF:6.789; C:09)
53. Naik, M.M., Naik, S.P., Dubey, S. K., Bhat, C. and L.S. Charya 2018. Enhanced
exopolysaccharide production and biofilm forming ability in methicillin resistant
Staphylococcus sciuri isolated from dairy in response to acyl homoserine lactone(AHL).
Journal of Food Science and Technology 55:2087-2094. (IF:2.701; C: 07).
54. Shamim, K.,Sharma, J., Mutnale, M., Dubey, S. K. and S. Mujawar 2018.
Characterization of a metagenomic serine metalloprotease and molecular
docking studies. Process Biochemistry 71: 69-75. (IF:3.757; C: 03)
55. Salkar, K., Gadgil, V., S. K. Dubey, Naik, Milind Mohan and R. R. Pandey 2018.
Largest freshwater lake ‘ Loktak’ in Manipur needs urgent conservation. Current
Science 114(12): 2436-37. (IF:0.9; C: 03)
58. Mujawar, Sajiya Yusuf, Vaigankar, Diviya Chandrakant and Santosh Kumar Dubey
2021. Biological characterization of Bacillus flexus strain SSAI1 transforming highly
toxic arsenite to less toxic arsenate mediated by periplasmic arsenite oxidase enzyme
encoded by aioAB genes. BioMetals 34: 895-907. DOI: 10.1007/s10534-021-00316-x.(
(IF:2.949)
59. Shraddha Pandey, Nikee Awasthee, Anusmita Shekher, Lal Chand Rai, Subash Chandra
Gupta and Santosh Kumar Dubey 2021. Biogenic synthesis and characterization of
selenium nanoparticles and their applications with special reference to antibacterial,
antioxidant, anticancer and photocatalytic activity. Bioprocess and Biosystems
Engineering 44: 2679–2696. DOI : 10.1007/s00449-021-02637-0 (IF:3.221)
2. Shraddha Pandey, Santosh Kumar Dubey and Lal Chand Rai 2021. Cyanobacteria:
miniature factory for green synthesis of nanomaterials. Biometals.
3. L. C. Rai, Shilpi Singh, Ruchi Rai, Krishna Kumar Rai, Alka Shankar, Alok Shrivastava and
Santosh Kumar Dubey 2021. Comparative analysis of stress tolerance of four Dps proteins
of Anabaena sp. PCC 7120 and molecular characterization of All4145. Algal Research.
Additional Information:
VISITS ABROAD
TECHNICAL EXPERTISE
Gene cloning, Vector Construction, Genomic and cDNA library construction and
screening, PCR, RT–PCR, DNA Sequencing (wet method)
Southern Blot, Northern Blot, SDS–PAGE, Anionic PAGE & Zymography, Western Blot,
Recombinant protein production using E. coli BL21
Tissue Culture of insect cells (sf9) and mammalian cell lines
Recombinant protein production using sf 9 insect cells (Baculovirus expression vector
system).
Recombinant protein purification by Column chromatography and FPLC
Gas chromatography (Acetylene reduction assay for nitrogenase)
PROGRAMME CO-ORDINATOR
Training of UG teachers in ‘Industrial Microbiology’, Sponsored by UGC, Govt. of India
(1997).
7th Refresher course in ‘Marine Microbiology’ at Goa University, Sponsored by UGC,
Govt. of India (1999).
UGC sponsored Innovative - M.Sc programme in Marine Microbiology in Department
of Microbiology, Goa University (June 2012 - May 2017).