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Experiment - 2

m.pharm practical

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63 views3 pages

Experiment - 2

m.pharm practical

Uploaded by

pandeykriti9
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXPERIMENT:-02

AIM: To Report the amount of Paracetamol present in sample by single point method.

REFERENCE: Chatwal GR. Anand S 9(2002) Instrumental Methods of Chemical Analysis (5th
edition), Himalaya Publishing House, New Delhi; Second edition of I.P. published in 1996.

REQUIREMENTS: Paracetamol, NaOH, Distilled Water, Volumetric flask, Beaker, Measuring


cylinder, Pipette

THEORY:

UV spectroscopy or UV–visible spectrophotometry (UV–Vis or UV/Vis) refers to absorption


spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible
regions of the electromagnetic spectrum. This means it uses light in the visible and adjacent
ranges. The absorption or reflectance in the visible range directly affects the perceived color of
the chemicals involved. In this region of the spectrum, atoms and molecules undergo electronic
transitions. Absorption spectroscopy is complementary to fluorescence spectroscopy, in that
fluorescence deals with transitions of electrons from the excited state to the ground state, while
absorption measures transitions from the ground state to the excited state.

DISCUSSION:

Paracetamol is 4-hydroxy acetanilide. It is in analgesic and antipyretic. Paracetamol, also known


as acetaminophen, is a medication used to treat fever and mild to moderate pain. At a standard
dose, paracetamol only slightly decreases body temperature; it is inferior to ibuprofen in that
respect, and the benefits of its use for fever are unclear. Paracetamol may relieve pain in acute
mild migraine but only slightly in episodic tension headache. However, the
aspirin/paracetamol/caffeine combination helps with both conditions where the pain is mild and
is recommended as a first-line treatment for them. Paracetamol is effective for post-surgical pain,
but it is inferior to ibuprofen. The paracetamol/ibuprofen combination provides further increase
in potency and is superior to either drug alone. The pain relief paracetamol provides in
osteoarthritis is small and clinically insignificant. The evidence in its favor for the use in low
back pain, cancer pain and neuropathic pain is insufficient.
• Dose: 500mg to 1g every 4 to 6 hours, up to 4g daily in divided doses.

• Paracetamol can be assayed by measuring absorbance of its solution in 0.1 N


NaOH at 257nm (U.V Spectrometry).

PROCEDURE:

PREPRATION OF STANDARD SOLUTION

1. Weigh 10mg paracetamol and transfer to 100ml volumetric flask containing 20ml
of 0.1N NaOH.
2. Dilute up to the mark with distilled water

3. Pipette out 1ml of above solution in 10ml volumetric flask and dilute up to 10ml with
distilled water.
PREPRATION OF TEST SOLUTION

1. Weigh 5 tablets of paracetamol.

2. Weigh tablet powder equivalent to 10mg paracetamol and transfer to 100ml volumetric
flask.

3. Dissolve it in 20ml 0.1 N NaOH and dilute up to mark with distilled water.

4. Pipette out 1ml of prepared solution in 10ml volumetric flask and dilute 10ml with
distilled water.
5. Measure absorbance of standard and test solutions at 240nm.

CALCULATION:

To make 0.1N NaOH


W=NEV/1000
W=0.1*40*100/1000
W=0.4 gm
Calculation for test solution.
Average weight of 5 tablet tablet:- 600+610+610+620+620 / 5 = 612 mg
Thus, 612 mg of tablet powder contain 500 mg pcm
X gm of tablet powder = 10 mg of pcm
X = 650 * 10 / 500
X=12.24mg
 Concentration of diluted standard solution.
Concentration of standard: -
 10mg-------100ml
 1mg--------10ml
 1000µg-----10ml
 100 µg-----1ml

Concentration of dilution standard: -


1ml -------10ml
C1V1=C2V2
100*1ml=C2*10
C2= 100/10
dil.C2=10µg/ml
 Concentration of dilution test solution.
 Astd / Atest = Cdil.std/ Cdil.test
 0.8308/0.7667=10/Cdil.test
 Cdil.test = 9.2340 µg /ml
 Concentration of test solution = Dilution factor *concentration of dilution test
= 1000 * 9.234
= 9234µg/ml

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